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Transcriptional regulation of MdmiR285N microRNA in apple(Malus x domestica)and the heterologous plant system Arabidopsis thaliana

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Horticulture Research 2020年第1期7卷 1501-1514页

作者： Valerio Pompili Stefano Piazza Mingai Li claudio varotto Mickael Malnoy Department of Genomics and Biology of Fruit Crops Research and Innovation CentreFondazione Edmund MachVia E.Mach 1San Michele all’Adige 38010Italy Department of Agricultural FoodEnvironmental and Animal SciencesUniversitàdegli Studi di UdineVia delle Scienze 206Udine 33100Italy Department of Biodiversity and Molecular Ecology Research and Innovation CentreFondazione Edmund MachVia E.Mach 1San Michele all’Adige 38010Italy

Malus x domestica microRNA MdmiR285N is a potential key regulator of plant immunity,as it has been predicted to target 35 RNA transcripts coding for different disease resistance proteins involved in plant defense to *** this study,the promoter region of MdmiR285N was isolated from the apple genome and analyzed in silico to detect potential regulatory regions controlling its transcription.A complex network of putative regulatory elements involved in plant growth and development,and in response to different hormones and stress conditions,was *** of theβ-Glucoronidase(GUS)reporter gene driven by the promoter of MdmiR285N was examined in transgenic apple,demonstrating that MdmiR285N was expressed during the vegetative growth ***,in transgenic Arabidopsis thaliana,spatial and temporal patterns of GUS expression revealed that MdmiR285N was differentially regulated during seed germination,vegetative phase change,and reproductive *** elucidate the role of MdmiR285N in plant immunity,MdmiR285N expression in wild-type apple plants and GUS activity in transgenic apple and Arabidopsis thaliana plants were monitored in response to Erwinia amylovora and Pseudomonas syringae *** DC3000.A significant decrease of MdmiR285N levels and GUS expression was observed during host-pathogen ***,these data suggest that MdmiR285N is involved in the biotic stress response,plant growth,and reproductive development.

关键词： plant Arabidopsis immunity

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The Developmental and Physiological Traits of Rare and Threatened Moss Physcomitrium eurystomum Sendtn. (Funariaceae) Valuable for Its Conservation

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 2949-2961页

作者： Djordje P.Bozovic Anja Rimac Milorad M.Vujicic Pragya Singh Michal Goga Mingai Li claudio varotto Aneta D.Sabovljevic Marko S.Sabovljevic Institute of Botany and Botanical Garden Jevremovac Faculty of BiologyUniversity of BelgradeBelgrade11000Serbia Biodiversity Ecology and Environment AreaResearch and Innovation Centre of Fondazione Edmund MachSan Michele all’Adige(TN)38098Italy Division of Botany Department of BiologyFaculty of ScienceUniversity of ZagrebZagreb10000Croatia Center of Plant Biotechnology and Conservation(CPBC) Belgrade11000Serbia Institute of Biology and Ecology Department of Plant BiologyFaculty of SciencePavol Jozef Safarik University in KosiceKosice04001Slovakia NBFC National Biodiversity Future CenterPalermo90133Italy

Physcomitrium eurystomum *** a very rare European ephemeral funaroid *** entire European population of this species is considered threatened and it is red-listed in many regions and *** addition to being recognized as threatened and included in nature conservation legislation,it also requires active protec-tion *** study aims to contribute to effective conservation practices for *** con-servation physiology tests were carried out to propagate this species to achieve a reliable procedure for biomass production and the potential reintroduction of *** situ tests,both in vitro and ex vitro,were carried out to determine the optimal method for spore production under laboratory conditions,considering that the spores are the best propagules for the reintroduction of the *** important outcome of this research is a deeper understanding of the role of the spore bank of this species for its survival in a variable *** additional characterizations of the biology of this ephemeral moss species are also discussed,such as the functioning of the photosynthetic apparatus of the vegetative *** phases to clarify the transition to the reproductive *** is proposed that both types of sporophytes be used in conservation practices due to viable spore bank self-sustainability in rapidly changing environmental conditions.

关键词： Physcomitrium Spore germination photosynthesis ﬂuorescence in vitro ex situ

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Interleukin 1βreceptor and synaptic dysfunction in recurrent brain infection with Herpes simplex virus type-1

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Neural Regeneration Research 2025年第2期20卷 416-423页

作者： Roberto Piacentini claudio Grassi Department of Neuroscience UniversitàCattolica del Sacro CuoreRomeItaly Fondazione Policlinico Universitario A.Gemelli IRCCSRomeItaly

Several experimental evidence suggests a link between brain Herpes simplex virus type-1 infection and the occurrence of Alzheimer’s ***,the molecular mechanisms underlying this association are not completely *** the molecular mediators of synaptic and cognitive dysfunction occurring after Herpes simplex virus type-1 infection and reactivation in the brain neuroinflammatory cytokines seem to occupy a central ***,we specifically reviewed literature reports dealing with the impact of neuroinflammation on synaptic dysfunction observed after recurrent Herpes simplex virus type-1 reactivation in the brain,highlighting the role of interleukins and,in particular,interleukin 1βas a possible target against Herpes simplex virus type-1-induced neuronal dysfunctions.

关键词： herpes simplex virus type 1 interleukin 1β microglia neuroinflammation synaptic dysfunction

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Proteostasis failure:at the intersection between aging and Alzheimer's disease

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Neural Regeneration Research 2025年第11期20卷 3221-3222页

作者： Natalia Poblete claudio Hetz Center for Geroscience Brain Health and MetabolismSantiagoChile Biomedical Neuroscience Institute Faculty of MedicineUniversity of ChileSantiagoChile Program of Cellular and Molecular Biology Institute of Biomedical SciencesFaculty of MedicineUniversity of ChileSantiagoChile Buck Institute for Research on Aging NovatoCAUSA

Lifestyle and demographics of the world's population are causing serious health problems impacting the brain,increasing the incidence of Alzheimer's disease(AD)and other types of *** we have gained important insights into the pathogenic mechanisms of AD,only palliative care is available to *** is characterized by the abnormal deposition of protein aggregates in the brain formed by amyloid β and hyper-phosphorylated,Tau in addition to neuroinflammation.

关键词： Alzheimer amyloid stasis

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Localization and shock waves in curved manifolds

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Science Bulletin 2016年第7期61卷 570-575页

作者： claudio Conti Institute for Complex Systems National Research Council(ISC-CNR)Via dei Taurini 1900185 Rome ITItaly Department of Physics University SapienzaPiazzale AldoMoro 500185 Rome ITItaly

The investigation of the interplay between geometry and nonlinearity may open the road to the control of extreme waves. We study three-dimensional localization and dispersive shocks in a bent cigar shaped potential by the nonlinear Schro¨ dinger equation. At high bending and high nonlinearity, topological trapping is frustrated by the generation of curved wave-breaking. Four-dimensional parallel simulations confirm the theoretical model. This work may contribute to novel devices based on geometrically constrained highly nonlinear dynamics and tests and analogs of fundamental physical theories in curved space.

关键词： Nonlinear waves Shock wavesNonlinear optics Curvature Bose-Einsteincondensation

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Linear and Nonlinear Anderson Localization in a Curved Potential

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Chinese Physics Letters 2014年第3期31卷 27-29页

作者： claudio Conti Department of Physics University Sapienza Piazzale Aldo Moro 5 Rome 00185 Italy

Disorder induced localization in the presence of nonlinearity and curvature is investigated. The time-resolved three-dimensional expansion of a wave packet in a bent cigar shaped potential with a focusing Kerr-like interaction term and Gaussian disorder is numerically analyzed. A self-consistent analytical theory, in which randomness, nonlinearity and geometry are determined by a single scaling parameter, is reported, and it is shown that curvature enhances localization.

关键词： WAVE packets RESEARCH NONLINEAR theories CURVATURE LOCALIZATION (Mathematics) PHYSICS research

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Interaction between animal personality and animal cognition

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Current Zoology 2011年第4期57卷 491-498页

作者： claudio CARERE Charles LOCURTO Department of Ecology and Sustainable Economic Development Centro Ittiogenico Sperimentale Universith degli Studi della Tuscia Borgo Le Saline 01016 Tarquinia (Vt) Italy Department of Psychology College of The Holy Cross 1 College St PO Box 75A Worcester MA 01610 USA

The study of animal personality has attracted considerable attention, as it has revealed a number of similarities in personality between humans and several nonhuman species. At the same time the adaptive value and evolutionary maintenance of different personalities are the subject of debate. Since Pavlov＇s work on dogs, students of comparative cognition have been aware that animals display vast individual differences on cognitive tasks, and that these differences may not be entirely accounted for differences in cognitive abilities. Here, we argue that personality is an important source of variation that may affect cognitive performance and we hypothesise mutual influences between personality and cognition across an individual＇s lifespan. In particular, we suggest that： 1） personality profiles may be markers of different cognitive styles; 2） success or failure in cognitive tasks could affect different personalities differently; 3） ontogenetic changes of personality profiles could be reflected in changes in cognitive performance. The study of such interplay has implications in animal welfare as well as in neuroscience and in translational medicine [Current Zoology 57 （4）： 491-498, 2011].

关键词： Animal personality Coping style Cognition

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CO_(2) conversion to solar fuels and chemicals:Opening the new paths

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Journal of Energy Chemistry 2024年第4期91卷 680-683页

作者： Gabriele Centi claudio Ampelli Department of Chemical BiologicalPharmaceutical and Environmental Sciences(ChiBioFarAm)University of MessinaERIC aisbl and CASPE/INSTMV.le F.Stagno d'Alcontres 3198166 MessinaItaly

This future article discusses the new prospects and directions of CO_(2)conversion via the photo-electrocatalytic(PEC)*** second(2nd)generation solar fuels and chemicals(SFs)are generated directly in PEC systems via electrons/protons reactions without forming molecular H_(2)as an intermediate,overcoming the thermodynamics limitations and practical issues encountered for electro-fuels produced by multistep thermocatalytic processes(***_(2)conversion with H_(2)coming from water electrolysis).A distributed and decentralized production of SFs requires very compact,highly integrated,and intensified *** the existing reactors of advanced design(based on artificial leaves or photosynthesis),the integrated photovoltaic plus electrocatalytic(PV-EC)device is the only system(demonstrated at large scale)to produce SFs with high solar-to-fuel(STF)***,while the literature indicates STF efficiency as the main(and only)measure of process performance,we remark here the need to refer to productivity(in terms of current density)and make tests with reliable flow PEC systems(with electrodes of at least 5–10 cm^(2))to accelerate the scaling-up *** approaches that minimize downstream separation costs is also *** limitations exist in PEC systems,but most can be overcome by proper electrode and cell engineering,thus going beyond the properties of the *** examples of current developments,we present the progress of(i)artificial leaf/tree devices for green H_(2)distributed production and(ii)a PEC device producing the same chemicals at both cathode and anode parts without downstream operations for green solvent distributed *** on these developments,future directions,such as producing fertilizers and food components from the air,are *** aim is to provide new ideas and research directions from a personal perspective.

关键词： Solar fuels Artificial leaf PEC devices PV-EC devices Cell engineering green H_(2) Chemicals from theair

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Conjugate Heat Transfer Characterization in Cooling Channels

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Journal of Thermal Science 2012年第3期21卷 286-294页

作者： Beni Cukurel Tony Arts claudio Selcan von Karman Institute for Fluid Dynamics Turbomachinery Department Chaussée de Waterloo

Cooling technology of gas turbine blades,primarily ensured via internal forced convection,is aimed towards withdrawing thermal energy from the *** promote heat exchange,the walls of internal cooling passages are lined with repeated geometrical flow disturbance elements and surface *** the heat transfer at the expense of increased pressure loss;the goal is to obtain the highest possible cooling effectiveness at the lowest possible pressure drop *** cooling channel heat transfer problem involves convection in the fluid domain and conduction in the *** coupled behavior is known as conjugate heat *** experimental study models the effects of conduction coupling on convective heat transfer by applying iso-heat-flux boundary condition at the external side of a scaled serpentine *** involve local temperature measurements performed by Infrared Thermography over flat and ribbed slab *** number distributions along the wetted surface are obtained by means of heat flux distributions,computed from an energy balance within the metal *** the flat plate experiments,the effect of conjugate boundary condition on heat transfer is estimated to be in the order of 3%.In the ribbed channel case,the normalized Nusselt number distributions are compared with the basic flow *** the findings with other conjugate and convective iso-heat-flux literature,a high degree of overall correlation is evident.

关键词： conjugate heat transfer turbine cooling channel convection infrared thermography

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Amine-rich carbon nitride nanoparticles:Synthesis,covalent functionalization with proteins and application in a fluorescence quenching assay

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Nano Research 2019年第8期12卷 1862-1870页

作者： Gabriele Capilli Simone Cavalera Laura Anfossi Cristina Giovannoli Marco Minella claudio Baggiani claudio Minero Department of Chemistry and NIS Center of Excellence University of Torino10125 TorinoItaly

Carbon nitride nanoparticles (CNNPs) have been employed as fluorescent sensing tools owing to their unique features, e.g. low cost production, high stability in water and high photoluminescence quantum yield. Here, an easy and versatile synthetic approach was exploited to design fluorescent nanoparticles with surface functionalities suitable for covalent binding to bioligands. High hydrophilic, brightly fluorescent CNNPs, rich of superficial amines, were obtained from the thermal condensation of urea and lysine (CNNP^Lys) and by tuning the precursor ratio and the heating time. Structure and size of the functionalized nanoparticles were characterized through infrared (IR) spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS). Their optical properties were studied by ultraviolet-visible (UV-Vis) and fluorescence spectroscopy. The superficial primary amino groups, furnished by the lysine co-precursor, enabled for covalently linking CNNP^Lys to model proteins. The CNNP^Lys-protein conjugates excited under UV irradiation emit in the 400-450 nm visible range (quantum yield 24%). The applicability of CNNP^Lys as novel fluorescent probes was demonstrated by a fluorescence quenching assay, in which gold nanoparticles (GNPs) were attached to Staphylococcal protein A and employed to quench the CNNP^Lys fluorescence by Forster resonant energy transfer (FRET). The quenching occurred upon formation of the specific binding between the GNP-linked protein A and CNNP^Lys-tagged immunoglobulins, while the inhibition of the binding resulted in the recovery of CNNP^Lys luminescence. The synthetic strategy, based on combining a "conjugated polymer"-forming unit (urea) and a co-precursor able to provide the desired functional group (lysine), allows designing innovative materials for the development of new generation fluorescence biosensors in which easily functionalized fluorophores are needed.

关键词： fluorescent probe carbon nitride nanoparticles quenching assay fluorescent materials g-C3N4 Forster resonant energy transfer (FRET)

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