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Dissemination of Resistance Integrons and Genes Coding for Blse and Cabapenemases in the Urban Drainage Network in Cote d’Ivoire

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Advances in Microbiology 2024年第5期14卷 268-286页

作者： Coulibaly Kalpy Julien Diaby Aboubakar Sidik Vakou N’dri Sabine M’bengue Gbonon Valérie Carole claon jean stephane Yao Kouamé Eric Gnali Gbohounou Fabrice Yéo Yéfougnini Bagré Issa Djaman Allico Joseph Dosso Mireille Pasteur Institute of Ivory Coast Abidjan C&#244te d&#8217Ivoire Fé lix Houphou&#235t-Boigny University Abidjan C&#244te d&#8217Ivoire

Antibiotic resistance has become a major threat to human health worldwide. Environment, particularly the water environment, has long been overlooked as a player in the antibiotic resistance cycle, although its role remains unclear. These can provide an ideal setting for the acquisition and dissemination of antibiotic resistance, as they are frequently affected by anthropogenic activities. The objective of this study was to establish a diffusion map of resistance integrons used as genetic markers of resistance associated with antibiotic resistance conferring genes (ARGs). Total DNA extracts from non-cultivable bacterial communities were used for the analyses. These communities were obtained from wastewater samples from 14 sites upstream and downstream of drainage channels or effluents in the cities of Abidjan, Bouaké, and Yamoussoukro. The results obtained correspond to the number of positives among the treated samples (n = 39). Among the genetic markers of dissemination, class 1 integrons were the most evident in 94.8% of samples in Abidjan (93.3%), Bouaké (100%) and Yamoussoukro (91.6%). Class 2 integrons and class 3 integrons were found respectively in 41% and 51% of all samples. Genes coding for β-lactamases and blaTEM was identified in almost all samples at a rate of 97.4%. A co-presence of the three genes blaTEM, blaSHV and blaCTX-M is also remarkable in the sites of the city of Yamoussoukro. Among the genes coding for carbapenemases, only blaKPC 17.94%, blaNDM 30.76% and blaOXA48 38.46% were detected in the samples.

关键词： Antibiotic Resistance Wastewater Resistance Integrons (RIs) Resistance Genes

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Distribution and Contamination of Arsenic in Fish, Gastropods and Bivalves in the Aby and Tendo Lagoons in East of Ivory Coast

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Journal of Environmental Protection 2024年第3期15卷 246-264页

作者： Kouakou Serge Kouassi Stéphane jean claon Kossonou Roland N’Guettia Séri Kipré Laurent Dakouo Guei Jokebed Djaman Allico Joseph Laboratory of Biology and Health University of F&eacutelix Houphou&eumlt-Boigny Abidjan C&ocircte d’Ivoire Department of Analytical Sciences and Public Health Faculty of Pharmaceutical and Biological Sciences University of F&eacutelix Houphou&eumlt-Boigny Abidjan C&ocircte d’Ivoire Laboratory of Environmental Sciences (LSE) University of Nangui Abrogoua Abidjan C&ocircte d’Ivoire Department of Medical and Fundamental Biochemistry Institut Pasteur of C&ocircte d’Ivoire Abidjan C&ocircte d’Ivoire

Lagoons are ecosystems for biodiversity and the livelihoods of coastal communities. The main objective of the study was to analyze the variability of arsenic concentrations in gastropods and bivalves in the Aby and Tendo lagoons, taking into account spatial, seasonal and hydrological variations. The study was carried out in four stages spread over two successive hydroclimatic cycles, including two seasons during the rainy season and two more during the dry season. The samples were taken in two areas of the Aby and Tendo lagoons. Arsenic levels were measured by ICP-MS. The results showed that mean arsenic concentrations in the muscles of organisms in Aby Lagoon ranged from 0.01 to 1.26 μg As/g, with a mean and median of 0.17 and 0.06 μg As/g, respectively. Fish had the highest levels of arsenic, followed by crustaceans, while molluscs and plants had lower and comparable concentrations of As. Arsenic concentrations in tilapia and jawbones varied significantly between sites and seasons, with higher concentrations at Tendo and during the rainy season. Arsenic concentrations in gastropods and bivalves were significantly higher than those of other species, with averages of 0.74 and 1.03 mg As/kg, respectively.

关键词： Arsenic Pollution Fish Gastropods Lagoons Health Effect

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Mercury Levels Assessment in the Population of Aby and Frambo Villages in the Vinicity of Aby Lagoon in Côte d’Ivoire (West Africa)

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Journal of Water Resource and Protection 2024年第3期16卷 219-232页

作者： Stéphane jean claon Serge Kouakou Kouassi Nina Laurette Ahouéfa Laurent Kipré Seri Arsène M’bassidjé Seka Joseph Allico Djaman Luc Kouakou Kouadio Analytical Sciences and Public Health Department University of Félix Houphou&eumlt-Boigny Abidjan C&ocircte d'Ivoire Water Quality Control and Analysis Laboratory (LACQUE) Office National de l’Eau Potable (ONEP) Abidjan C&ocircte d’Ivoire Biology and Health Laboratory University of Félix Houphou&eumlt-Boigny Abidjan C&ocircte d’Ivoire Laboratory of Nutrition and Food Safety Department of Science and Food Technology Nangui Abrogoua University Abidjan C&ocircte d'Ivoire Department of Medical and Fundamental Biochemistry Institut Pasteur of C&ocircte d'Ivoire Abidjan C&ocircte d’Ivoire Laboratory of Environmental Sciences (LSE) University of Nangui Abrogoua Abidjan C&ocircte d’Ivoire

The Minamata Convention in the aim to protect human life and the environment, seeks to reduce mercury (Hg) by monitoring it concentrations in the environment. Artisanal and Small-scale Gold Mining (ASGM) has been identified as the most important anthropogenic source of human exposure to Hg. In this context, the main goal of this study was to assess the level of mercury in hair of two (2) populations living along two lagoons respectively Aby and Tendo, in Ivory Coast. To reach this goal, hair samples of 138 residents were collected and analyzed by using Cold Vapor Atomic Fluorescence Spectrophotometry (CV-AFS) technique for mercury concentration. Results showed that for the entire population the Hg mean was 2.34 μg/g. Also, they were ranged between 0.15 to 8.53 μg/g and presented substantial differences amongst the villages. In Aby village, we observed the highest Hg concentrations (Mean = 2.62 μg/g). Our findings showed that almost the entire sample group (82%) exceed the USEPA recommended limit, furthermore 56% of them exceed the normal level of WHO and 2% of the respondent has the unhealthy levels of mercury (≥6 μg Hg/g) of hair by WHO standards. Gender differences in hair mercury varies greatly among reports. Lower levels in women’s hair compared to men were reported in the both village. Considering age, the lowest concentrations were observed with children. However, when we take in account the age groups, data suggested that the most exposed sub-population of [18-29] years old is from Aby village in opposite at Frambo village, the same case those who were ≥ 40 years old. It’s convenient to note that, the proportion of Mercury levels would not to be neglected among studied population especially with the resident from Aby village. So, some measures need to be taken at the political level to control mercury contamination.

关键词： Mercury Hair ASGM Population Exposure Côte d’Ivoire

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Assessment of Selenium Contamination in Sediments of the Aby and Tendo Lagoons in Côte d’Ivoire

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International Journal of Geosciences 2024年第4期15卷 334-349页

作者： Stéphane jean claon Kouakou Serge Kouassi Kossonou Roland N’Guettia M’bassidjé Arsène Seka Guei Jokebed Dakouo Allico Joseph Djaman Kouakou Luc Kouadio Analytical Sciences and Public Health Department University of F&#233lix Houphou&#235t-Boigny Abidjan C&#244te d&#8217Ivoire Water Quality Control and Analysis Laboratory (LACQUE) Office National de l&#8217Eau Potable (ONEP) Abidjan C&#244te d&#8217Ivoire Biology and Health Laboratory University of F&#233lix Houphou&#235t-Boigny Abidjan C&#244te d&#8217Ivoire Laboratory of Environmental Sciences (LSE) University of Nangui Abrogoua Abidjan C&#244te d&#8217Ivoire Department of Medical and Fundamental Biochemistry Institut Pasteur of C&#244te d&#8217Ivoire Abidjan C&#244te d&#8217Ivoire

Selenium is a trace element that can have both beneficial and harmful effects on aquatic life. The Aby Lagoon is a coastal environment in Côte d’Ivoire that receives selenium inputs from various natural and anthropogenic sources. The aim of this study was to assess the levels of selenium in the sediments of the Aby Lagoon and its tributaries, the Tanoe River and the Tendo Lagoon, and to examine the spatial and seasonal variations of selenium concentrations. Sediment samples were collected from different sites and seasons, and selenium concentrations were measured by atomic fluorescence spectrometry. The results showed that the average concentration of selenium in the sediments of the Aby Lagoon was 0.82 mg/kg, indicating moderate contamination. The concentration of selenium varied between sites and seasons, with higher values in the channel of the Tendo Lagoon and during the dry season. The study highlighted the complexity of selenium dynamics in aquatic ecosystems, and the need to take into account seasonal and spatial variability as well as interactions between environmental factors. The study also suggested potential ecotoxicological risks for some sensitive organisms in certain areas of the lagoon. This study contributes to the knowledge of the dynamics of selenium in lagoon ecosystems and to the assessment of the environmental risks associated with its presence.

关键词： Tropical Estuarine Selenium Sediments Aby Lagoon Seasonal Distribution

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Assessment of Nitrates and Nitrites in Borehole Water from the Southern and the Northern Region of Côte d’Ivoire (West Africa)

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Open Journal of Modern Hydrology 2024年第2期14卷 87-105页

作者： jean Stéphane claon Kouassi Kouakou Serge Sérikipré Laurent Seka M’Bassidjé Arsène N’Guettia Kossonou Roland Traoré Aïcha Djamanallico Joseph Kouadio Kouakou Luc Analytical Sciences and Public Health Department University of Félix Houphou&#235t-Boigny Abidjan C&#244te d’Ivoire Water Quality Control and Analysis Laboratory (LACQUE) Office National de l’Eau Potable (ONEP) Abidjan C&#244te d’Ivoire Biology and Health Laboratory University of Félix Houphou&#235t-Boigny Abidjan C&#244te d’Ivoire Department of Medical and Fundamental Biochemistry Institut Pasteur of C&#244te d’Ivoire Abidjan C&#244te d’Ivoire Laboratory of Environmental Sciences (LSE) University of Nangui Abrogoua Abidjan C&#244te d’Ivoire

This study aimed to evaluate the quality of water from village boreholes by measuring physicochemical parameters such as nitrates, nitrites, and total organic carbon (TOC). Forty-five (45) village pumps from the Southern (Basse Côte) and the Northern (Korhogo) region of Cte d’Ivoire (west Africa) were sampled. Physicochemical parameters such as temperature, pH, conductivity at 25˚C, and turbidity were determined in situ, while nitrite and nitrate were analyzed according to ISO 10304-1 (2007) standard and total organic carbon (TOC) by NF EN 1484 (1997) standard. The results showed that the borehole waters of the Basse Côte and Korhogo analyzed are acidic, with an average temperature of 27.51˚C ± 0.16˚C and 29.95˚C ± 0.51˚C respectively for the Basse Côte and Korhogo regions. The borehole waters of the Basse Côtedo not contain nitrites, while those of Korhogo have average nitrite contents of 0.32 mg/l. The average nitrate rate in the waters of the Basse Côte and Korhogo are 12.08 ± 2.11 mg/l and 11.03 ± 3.18 mg/l respectively. The average TOC concentration of the waters of the Basse Côte is 1.28 ± 0.32 mg/l and that of Korhogo is 0.56 ± 0.09 mg/L. The study showed that the borehole waters of the Basse Côte and Korhogo have average temperatures between 27.4˚C and 29.95˚C with a slightly acidic pH value and acceptable salinity. The TOC concentrations obtained at the different sampling points were all below the French standard (2 mg/L) except for certains pumps of the Basse Côte. The water samples from the Basse Côte were devoid of nitrite. On the other hand, those from Korhogo revealed the presence of nitrite. Also, the borehole waters of the regions of the Basse Côte and Korhogo contain relatively high nitrate contents, presumably due to anthropometric activity. Overall, our study on the quality of drinking water showed that the waters analyzed are in compliance with international standards and safe for consumption.

关键词： Drinking Water pH Turbidity TOC Nitrates Nitrites Health Effect

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Exposure of Mercury from Gold Ming Area: A Case Study in Tendo and Aby Lagoon in Côte d’Ivoire

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Journal of Environmental Protection 2022年第9期13卷 613-627页

作者： Stéphane jean claon Serge Kouakou Kouassi Séka Arsène M’bassidje Laurent Kipré Séri Luc Kouakou Kouadio Joseph Allico Djaman Public Health and Hydrology Laboratory Félix Houphou&euml t-Boigny University Abidjan C&ocirc te d’Ivoire Water Quality Control and Analysis Laboratory National Drinking Water Office Abidjan C&ocirc te d’Ivoire Biology and Health Laboratory Félix Houphou&euml t-Boigny University Abidjan C&ocirc te d’Ivoire Environmental Sciences Laboratory Nanguy Abrogoua University Abidjan C&ocirc te d’Ivoire Department of Medical and Fundamental Biochemistry Institut Pasteur of C&ocirc te d’Ivoire Abidjan C&ocirc te d’Ivoire

Lagoons of West African countries are seriously threatened by rapid artisanal and small-scale gold mining (ASGM) that exposes lagoons to mercury pollution. In this study, the mercury level in the sediments of the Tendo and Aby lagoons in Côte d’Ivoire had been evaluated. A total of 87 samples from 25 stations located on Tendo and Aby lagoons were analyzed by atomic fluorescence spectrometry. The mean Hg concentrations obtained in the sediments of Tendo and Aby lagoons were 0.89 ± 0.26 mg⋅kg-1 and 0.70 ± 0.18 mg⋅kg-1, respectively. Hg concentrations evaluated in the bays of Tendo and Aby during the dry season were 1.38 ± 0.45 mg⋅kg-1 and 1.07 ± 0.31 mg⋅kg-1, respectively. The minimum and maximum total Hg concentrations in the sediments from 25 stations were 0.04 and 3.56 mg⋅kg-1, respectively. Mercury contamination in lagoons during ASGM poses risks of pollution for the lagoon ecosystem and also poses health risks for the population living near these lagoons.

关键词： ASGM Mercury Sediments Côte d’Ivoire Pollution

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Assessment of left-ventricular diastolic function in pediatric intensive-care patients:a review of parameters and indications compared with those for adults

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World Journal of Pediatrics 2021年第1期17卷 21-30页

作者： Morgan Recher Astrid Botte Jerome Soquet jean-Benoit Baudelet François Godart stephane Leteurtre ULR 2694-METRICS:évaluation des Technologies de santéet des Pratiques Médicales Univ.LilleCHU Lille59000 LilleFrance Réanimation et Surveillance Continue Pédiatriques Hopital Jeanne de FlandreCHU de Lille59037 Lille CedexFrance Pediatric and Neonatal Intensive Care Unit CHU Bordeaux33076 BordeauxFrance Cardiac Surgery Unit CHU Lille59000 LilleFrance Congenital and Pediatric Cardiology Unit CHU Lille59000 LilleFrance

Background The incidence of diastolic heart failure has increased over *** evaluation of left-ventricular diastolic func-tion is complex,ongoing,and remains poorly performed in pediatric intensive-care *** study aimed to review the literature and to provide an update on the evaluation of left-ventricular diastolic function in adults and children in intensive *** sources We searched data from PubMed/***-two studies were *** pragmatic questions were identified:(1)What is the physiopathology of diastolic dysfunction?(2)Which tools are required to evaluate diastolic func-tion?(3)What are the echocardiographic criteria needed to evaluate diastolic function?(4)When should diastolic function be evaluated in pediatric intensive care?Results Early diastole allows characterization of relaxation,whereas compliance assessments and filling pressures are evalu-ated during late *** evolution of diastolic function differs between adults and *** in adults,decreased compliance occurs at the same time as delayed relaxation in *** function can be evaluated by Doppler *** echocardiographic criteria for ventricular relaxation include the E wave,E/A wave ratio,and isovolumic relaxation *** compliance can be assessed by the E/e'wave ratio,atrial volume,and Ap wave duration during pulmonary vein *** adult intensive-care patients,the E/e'ratio can be used as an index of tolerance for volume expan-sion in septic patients and to adjust the inotropic *** Clinical studies would allow some of these parameters to be validated for use in children in intensive care.

关键词： Diastole Echocardiography Heart failure Intensive-care unit Pediatric

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Investigation of dynamics of discrete framed structures by a numerical wave-based method and an analytical homogenization approach

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Chinese Journal of Aeronautics 2017年第1期30卷 66-74页

作者： Zhou Changwei Sun Xiangkun Mohamed Ichchou Abdel-Malek Zine jean-Pierre Lainé stephane Hans Claude Boutin Laboratory of Tribology and Systems Dynamics Ecole Centrale de Lyon Ecully 69134 France Institut Camille Jordan Ecole Centrale de Lyon Ecully 69134 France Laboratory of Tribology and Systems Dynamics Ecole Nationale des Travaux Publics de l'Etat Vaulx-en-Velinx 69518 France

In this article, the analytical homogenization method of periodic discrete media（HPDM）and the numerical condensed wave finite element method（CWFEM） are employed to study the longitudinal and transverse vibrations of framed structures. The valid frequency range of the HPDM is re-evaluated using the wave propagation feature identified by the CWFEM. The relative error of the wavenumber by the HPDM compared to that by the CWFEM is illustrated in functions of frequency and scale ratio. A parametric study on the thickness of the structure is carried out where the dispersion relation and the relative error are given for three different thicknesses. The dynamics of a finite structure such as natural frequency and forced response are also investigated using the HPDM and the CWFEM.

关键词： Framed structures Homogenization method Periodic structures Wave finite element method Wave propagation

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Impact of time to diagnosis on the occurrence of cardiogenic shock in MIS‑C post‑COVID-19 infection

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World Journal of Pediatrics 2023年第6期19卷 595-604页

作者： Said Bichali Mathilde Bonnet Marie‑Emilie Lampin jean‑Benoit Baudelet Heloise Reumaux Olivia Domanski Thameur Rakza Alexandre Delarue Morgan Recher Jerome Soquet Francois Dubos stephane Leteurtre Ali Houeijeh Francois Godart Pediatric Cardiology Univ.LilleCHU Lille2 Avenue Oscar Lambret59000 LilleFrance Pediatric Intensive Care Univ.LilleCHU Lille59000 LilleFrance ULR 2694‑Metrics:Evaluation des Technologies de Sante et des Pratiques Medicales Univ.Lille59000 LilleFrance Pediatric Rhumatology Univ.LilleCHU Lille59000 LilleFrance Cardiac Surgery Univ.LilleCHU Lille59000 LilleFrance Pediatric Emergency Univ.LilleCHU Lille59000 LilleFrance

Background In multisystem inflammatory syndrome in children(MIS-C),diagnostic delay could be associated with *** study aims to measure the time to diagnosis in MIS-C,assess its impact on the occurrence of cardiogenic shock,and specify its *** A single-center prospective cohort observational study was conducted between May 2020 and July 2022 at a tertiary care *** meeting the World Health Organization MIS-C criteria were included.A long time to diagnosis was defined as six days or *** on time to diagnosis were collected by two independent *** primary outcome was the occurrence of cardiogenic *** regression and receiver operating characteristic curve analysis were used for outcomes,and a Cox proportional hazards model was used for *** Totally 60 children were assessed for inclusion,and 31 were finally analyzed[52%males,median age 8.8(5.7-10.7)years].The median time to diagnosis was 5.3(4.2-6.2)*** univariable analysis,age above the median,time to diagnosis,high C-reactive protein,and high N-terminal pro-B-type natriuretic peptide(NT-proBNP)were associated with cardiogenic shock[odds ratio(OR)6.13(1.02-36.9),2.79(1.15-6.74),2.08(1.05-4.12),and 1.70(1.04-2.78),respectively].In multivariable analysis,time to diagnosis≥6 days was associated with cardiogenic shock[adjusted OR(aOR)21.2(1.98-227)].Time to diagnosis≥6 days had a sensitivity of 89% and a specificity of 77% in predicting cardiogenic shock;the addition of age>8 years and NT-proBNP at diagnosis≥11,254 ng/L increased the specificity to 91%.Independent determinants of short time to diagnosis were age<8.8 years[aHR 0.34(0.13-0.88)],short distance to tertiary care hospital[aHR 0.27(0.08-0.92)],and the late period of the COVID-19 pandemic[aHR 2.48(1.05-5.85)].Conclusions Time to diagnosis≥6 days was independently associated with cardiogenic shock in *** diagnosis and treatment are crucial to avoid the use of inotropes and

关键词： COVID-19 Delay in diagnosis Heart failure MIS-C SARS-CoV-2

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Anomalous moire pattern of graphene investigated by scanning tunneling microscopy： Evidence of graphene growth on oxidized Cu（111）

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Nano Research 2014年第1期7卷 154-162页

作者： Nicolas Reckinger Eloise Van Hooijdonk Frederic Joucken Anastasia V. Tyurnina stephane Lucas jean-Francois Colome~ Research Center in Physics of Matter and Radiation (PMR) University of Namur Rue de Bruxelles 6 1 B-5000 Namur Belgium

The growth of graphene on oriented (111) copper films has been achieved by atmospheric pressure chemical vapor deposition. The structural properties of as-produced graphene have been investigated by scanning tunneling microscopy. Anomalous moiré superstructures composed of well-defined linear periodic modulations have been observed. We report here on comprehensive and detailed studies of these particular moiré patterns present in the graphene topography revealing that, in certain conditions, the growth can occur on the oxygen-induced reconstructed copper surface and not directly on the oriented (111) copper film as expected.

关键词：扫描隧道显微镜氧化铜石墨生长常压化学气相沉积证据图案波纹

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