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Synthetic and Systems Biotechnology 2019年第1期4卷 57-66页

作者： Lorna Suckling ciaran mcfarlane Chelsea Sawyer Stephen P.Chambers Richard I.Kitney David W.McClymont Paul S.Freemont The London DNA Foundry SynbiCITEImperial College LondonLondonSW72AZUK Department of Life Sciences Imperial College LondonLondonSW72AZUK Department of Bioengineering Imperial College LondonLondonSW72AZUK Department of Medicine Imperial College LondonLondonSW72AZUK

High-throughput preparation of plasmid DNA libraries for next-generation sequencing(NGS)is an important capability for molecular biology *** particular,it is an essential quality control(QC)check when large numbers of plasmid variants are being ***,we describe the use of the Design of Experiments(DOE)methodology to optimise the miniaturised preparation of plasmid DNA libraries for NGS,using the Illumina®Nextera XT technology and the Labcyte Echo®acoustic liquid dispensing ***,we describe methods which can be implemented as a QC check for identifying the presence of genomic DNA(gDNA)in plasmid DNA samples and the subsequent shearing of the gDNA,which otherwise prevents the acoustic transfer of plasmid *** workflow enables the preparation of plasmid DNA libraries which yield high-quality sequencing data.

关键词： preparation otherwise shearing

Emergence of the Wallerian degeneration pathway as a mechanism of secondary brain injury

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Neural Regeneration Research 2021年第5期16卷 980-981页

作者： ciaran Scott Hill Andrea Loreto National Hospital for Neurology and Neurosurgery University College London HospitalsUCL Cancer InstituteUniversity College LondonLondonUK Jon Van Geest Centre for Brain Repair University of CambridgeCambridgeUK

Augustus Volney Wal ler was a renowned British neurophysiologist who birthed the axon degeneration field in 1850 by describing curdling and fragmentation of the glossopharyngeal and hypoglossal cranial nerves of a frog following a transection *** degeneration of axons after a transection injury is now known as Wallerian degeneration(WD).Waller’s work was expanded by Santiago Ramón y Cajal who described in detail the morphological stages of WD from monitory fragmentation of the axon and the granular disintegration of the neurofibrils to the final resorption of the axon.

关键词： degeneration nerves Wallerian

Fabrication of Nanoscale Active Plasmonic Elements Using Atomic Force Microscope Tip-Based Nanomachining

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Nanomanufacturing and Metrology 2022年第1期5卷 50-59页

作者： ciaran Barron Silas O'Toole Dominic Zerulla School of Physics University College DublinDublinIreland

Atomic force microscopy(AFM)is a widely adopted imaging and surface analysis technique that provides resolutions on the nanometer *** tip-based nanomachining has recently been adopted for the fabrication of arbitrarily shaped nanoscale structures.A major challenge of using AFM tip-based machining for the sculpting of nanoscale plasmonic structures is the build-up of displaced material along the sides of the *** we apply this nanomechanical machining method to create active plasmonic elements and present the strategy we have been using to avoid the formation of such ***,a number of post-manufacturing treatments that can potentially be used to reduce the amount of debris surrounding the fabricated structures are discussed.

关键词： Nanomechanical machining Nanoscale fabrication Plasmonics Atomic force microscope AFM

Seasonal exports of phosphorus from intensively fertilised nested grassland catchments

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Journal of Environmental Sciences 2013年第9期25卷 1847-1857页

作者： ciaran Lewis Rashad Rafique Nelius Foley Paul Leahy Gerard Morgan John Albertson Sandeep Kumar Gerard Kiely Department of Civil and Environmental Engineering University College Cork Department of Microbiology and Plant Biology University of Oklahoma Environmental Research Institute University College Cork Aquatic Services Unit Environmental Research Institute University College Cork Department of Civil and Environmental Engineering Duke University Department of Plant Science South Dakota State University

We carried out a one year （2002） study of phosphorus （P） loss from soil to water in three nested grassland catchments with known P input in chemical fertilizer and animal liquid slurry applications. Chemical fertilizer was applied to the grasslands between March and September and animal slurry was applied over the twelve months. The annual chemical P fertilizer applications for the 17 and 211 ha catchments were 16.4 and 23.7 kg P/ha respectively and the annual slurry applications were 10.7 and 14.0 kg P/ha, respectively. The annual total phosphorus （TP） export in stream-flow was 2.61, 2.48 and 1.61 kg P/ha for the 17, 211 and 1524 ha catchments, respectively, compared with a maximum permissible （by regulation） annual export of ca. 0.35 kg P/ha. The export rate （ratio of P export to P in land applications） was 9.6% and 6.6% from the 17 and 211 ha catchments, respectively. On average, 70% of stream flow and 85% of the P export occurred during the five wet months （October to February） indicating that when precipitation is much greater than evaporation, the hydrological conditions are most favourable for P export. However the soil quality and land use history may vary the results. Particulate P made up 22%, 43% and 37% of the TP export at the 17, 211 and 1524 ha catchment areas, respectively. As the chemical fertilizer was spread during the grass growth months （March to September）, it has less immediate impact on stream water quality than the slurry applications. We also show that as the catchment scale increases, the P concentrations and P export decrease, confirming dilution due to increasing rural catchment size. In the longer term, the excess P from fertilizer maintains high soil P levels, an antecedent condition favourable to P loss from soil to water. This study confirms the significant negative water quality impact of excess P applications, particularly liquid animal slurry applications in wet winter months. The findings suggest that restricted P application in w

关键词： phosphorus fertilizer slurry exports grassland land management water quality eutrophication

Bibliometric analysis of research on the effects of human immunodeficiency virus in orthopaedic and trauma surgery

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World Journal of Orthopedics 2021年第3期12卷 169-177页

作者： ciaran Brennan Maritz Laubscher Sithombo Maqungo Simon Matthew Graham Orthopaedic Research Unit University of Cape TownCape Town 7925South Africa Division of Orthopaedic Surgery Groote Schuur HospitalCape Town 7925South Africa Institute of Population Health Sciences University of LiverpoolLiverpool L97ALUnited Kingdom Department of Orthopaedic and Trauma Surgery Liverpool University Teaching Hospitals TrustLiverpool L97ALUnited Kingdom Department of Clinical Sciences Liverpool School of Tropical MedicineLiverpool L97ALUnited Kingdom

BACKGROUND There is little research investigating how human immunodeficiency virus(HIV)affects outcomes in orthopaedic *** advances in treatment,HIV has become a chronic health problem and the chance of orthopaedic surgeons encountering it in clinical practice is *** To ascertain the quantity and quality of peer-reviewed publications in orthopaedic journals about *** A search of the Web of Science database was carried out,identifying any articles relating to HIV published in orthopaedic *** were assessed for geographic origin and level of *** Of 48.7%of orthopaedic journals listed on the Web of Science database had published articles relating to *** were 168 articles about HIV in orthopaedic journals with only 40.5%(n=68)published in the time frame we analysed(January 2007 to September 2017).Very few articles came from lowincome countries and any articles published from that setting were *** of the articles were low level of *** There is a need for more high level orthopaedic and trauma research investigating the effects of HIV,particularly research from low-income countries,where higher level research will help to guide improvements in their treatment of its musculoskeletal manifestations and complications.

关键词： Human immunodeficiency virus Research Bibliometric analysis Analysis Orthopaedic

Prediction of voltage distribution using deep learning and identified key smart meter locations

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Energy and AI 2021年第4期6卷 31-40页

作者： Maizura Mokhtar Valentin Robu David Flynn ciaran Higgins Jim Whyte Caroline Loughran Fiona Fulton Smart System Research Group Heriot-Watt UniversityRiccarton CampusEH144ASEdinburghUK CWI National Research Institute for Mathematics and Computer Science1098XG AmsterdamThe Netherlands Algorithmics Group EEMCSDelft University of Technology2628XE DelftThe Netherlands Derryherk Ltd. Renfrewshire PA113BEUK Smart Meter Systems SP Energy NetworksGlasgow G25ADUK

The energy landscape for the Low-Voltage(LV)networks is undergoing rapid *** changes are driven by the increased penetration of distributed Low Carbon Technologies,both on the generation side(*** of micro-renewables)and demand side(*** vehicle charging).The previously passive‘fit-and-forget’approach to LV network management is becoming increasing inefficient to ensure its effective operation.A more agile approach to operation and planning is needed,that includes pro-active prediction and mitigation of risks to local sub-networks(such as risk of voltage deviations out of legal limits).The mass rollout of smart meters(SMs)and advances in metering infrastructure holds the promise for smarter network ***,many of the proposed methods require full observability,yet the expectation of being able to collect complete,error free data from every smart meter is unrealistic in operational ***,the smart meter(SM)roll-out has encountered significant issues,with the current voluntary nature of installation in the UK and in many other countries resulting in low-likelihood of full SM coverage for all LV *** with a comprehensive SM roll-out privacy restrictions,constrain data availability from *** address these issues,this paper proposes the use of a Deep Learning Neural Network architecture to predict the voltage distribution with partial SM coverage on actual network operator LV *** results show that SM measurements from key locations are sufficient for effective prediction of the voltage distribution,even without the use of the high granularity personal power demand data from individual customers.

关键词： Voltage prediction Smart meters Deep neural learning Distribution network operation Big Data Analytics Analytic methods in power networks Privacy-preserving data analysis

Women and exercise in aging

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Journal of Sport and Health Science 2014年第3期3卷 170-178页

作者： Kristina L.Kendall ciaran M.Fairman Department of Health and Kinesiology Georgia Southern University

Aging is associated with physiological declines, notably a decrease in bone mineral density （BMD） and lean body mass, with a concurrent increase in body fat and central adiposity. Interest in women and aging is of particular interest partly as a result of gender specific responses to aging, particularly as a result of menopause. It is possible that the onset of menopause may augment the physiological decline associated with aging and inactivity. More so, a higher incidence of metabolic syndrome （an accumulation of cardiovascular disease risk factors including obesity, low-density lipoprotein cholesterol, high blood pressure, and high fasting glucose） has been shown in middle-aged women during the postmenopausal period. This is due in part to the drastic changes in body composition, as previously discussed, but also a change in physical activity （PA） levels. Sarcopenia is an age related decrease in the cross-sectional area of skeletal muscle fibers that consequently leads to a decline in physical function, gait speed, balance, coordination, decreased BMD, and quality of life. PA plays an essential role in combating physiological decline associated with aging. Maintenance of adequate levels of PA can result in increased longevity and a reduced risk for metabolic disease along with other chronic diseases. The aim of this paper is to review relevant literature, examine current PA guidelines, and provide recom- mendations specific to women based on current research.

关键词： Aerobic Exercise prescription Flexibility Older adults Strength training

Cyber Babies： The Impact of Emerging Technology on the Developing Infant

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Psychology Research 2015年第9期5卷 504-518页

作者： ciaran Haughton Mary Aiken Carly Cheevers RCSI CyberPsychology Research Centre RCSI Institute of Leadership Dublin Ireland

可持续发展背景下的全球特种纸行业——专访Smithers美洲及亚太区副总裁ciaran Little先生和Smithers亚太区项目总监陆尉斌先生

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中华纸业 2024年第4期45卷 13-17页

作者：李玉峰 ciaran Little 陆尉斌不详 Smithers美洲及亚太区 Smithers亚太区

中华纸业:请介绍一下目前全球特种纸行业的发展状况?Smithers:首先,“特种纸”通常被认为更多的是商业分类而不是造纸行业的技术术语。基于史密瑟斯Smithers在造纸行业有广泛的历史背景和专业知识,我们很早推出了特种纸市场研究报告。... 详细信息

中华纸业:请介绍一下目前全球特种纸行业的发展状况?Smithers:首先,“特种纸”通常被认为更多的是商业分类而不是造纸行业的技术术语。基于史密瑟斯Smithers在造纸行业有广泛的历史背景和专业知识,我们很早推出了特种纸市场研究报告。与其他纸品不同,特种纸主要目的是增强产品的功能、加工或装饰用途,其特点包括其独特的涂层、添加剂和纸张结构。它们通常生产批量较小,成本效益更高,并且利润也相对更好。

关键词：成本效益市场研究报告特种纸造纸行业项目总监技术术语生产批量可持续发展

Non-responsive celiac disease in children on a gluten free diet

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World Journal of Gastroenterology 2021年第13期27卷 1311-1320页

作者： Gopal Veeraraghavan Amelie Therrien Maya Degroote Allison McKeown Paul D Mitchell Jocelyn A Silvester Daniel A Leffler Alan M Leichtner ciaran P Kelly Dascha C Weir Division of Gastroenterology Hepatology and NutritionBoston Children's HospitalBostonMA 02115United States The Celiac Center Department of Medicine and Division of GastroenterologyBeth Israel Deaconess Medical CenterBostonMA 02215United States Celiac Research Program Harvard Medical SchoolBostonMA 02115United States Institutional Centers for Clinical and Translational Research Boston Children's HospitalBostonMA 02115United States Gastrointestinal Therapeutics Takeda Pharmaceutical International CoCambridgeMA 02139United States

BACKGROUND Non-responsive celiac disease(NRCD) is defined as the persistence of symptoms in individuals with celiac disease(CeD) despite being on a gluten-free diet(GFD). There is scant literature about NRCD in the pediatric *** To determine the incidence, clinical characteristics and underlying causes of NRCD in *** Retrospective cohort study performed at Boston Children’s Hospital(BCH). Children < 18 years diagnosed with CeD by positive serology and duodenal biopsies compatible with Marsh Ⅲ histology between 2008 and 2012 were identified in the BCH’s Celiac Disease Program database. Medical records were longitudinally reviewed from the time of diagnosis through September 2015. NRCD was defined as persistent symptoms at 6 mo after the initiation of a GFD and causes of NRCD as well as symptom evolution were detailed. The children without symptoms at 6 mo(responders) were compared with the NRCD group. Additionally, presenting signs and symptoms at the time of diagnosis of CeD among the responders and NRCD patients were collected and compared to identify any potential predictors for NRCD at 6 mo of GFD *** Six hundred and sixteen children were included. Ninety-one(15%) met criteria for NRCD. Most were female(77%). Abdominal pain [odds ratio(OR) 1.8 95% confidence interval(CI) 1.1-2.9], constipation(OR 3.1 95%CI 1.9-4.9) and absence of abdominal distension(OR for abdominal distension 0.4 95%CI 0.1-0.98) at diagnosis were associated with NRCD. NRCD was attributed to a wide variety of diagnoses with gluten exposure(30%) and constipation(20%) being the most common causes. Other causes for NRCD included lactose intolerance(9%), gastroesophageal reflux(8%), functional abdominal pain(7%), irritable bowel syndrome(3%), depression/anxiety(3%), eosinophilic esophagitis(2%), food allergy(1%), eating disorder(1%), gastric ulcer with Helicobacter pylori(1%), lymphocytic colitis(1%), aerophagia(1%) and undetermined(13%). 64% of children with NRCD

关键词： Celiac disease Non-responsive celiac disease Children Gluten-free diet Constipation Abdominal pain