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Identification of differential metabolites in liquid diet fermented with Bacillus subtilis using gas chromatography time of flight mass spectrometry

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Animal Nutrition 2016年第4期2卷 351-356页

作者： Yuyong He chunxia mao Zhiyu Chen Hong Wen Wei Lu Huadong Wu Jiangxi Province Key Laboratory of Animal Nutrition Jiangxi Agricultural University Jiangxi Provincial Institute of Veterinary Drugs and Feed Control College of Animal Science and Technology Jiangxi Agricultural University

Growth and health responses of pigs fed fermented liquid diet are not always consistent and causes for this issue are still not very clear. Metabolites produced at different fermentation time points should be one of the most important contributors. However, currently no literatures about differential metabolites of fermented liquid diet are reported. The aim of this experiment was to explore the difference of metabolites in a fermented liquid diet between different fermentation time intervals. A total of eighteen samples that collected from Bacillus subtilis fermented liquid diet on days 7, 21 and 35 respectively were used for the identification of metabolites by gas chromatography time of flight mass spectrometry(GCTOF-MS). Fifteen differential metabolites including melibiose, sortitol, ribose, cellobiose, maltotriose,sorbose, iso maltose, maltose, fructose, D-glycerol-1-phosphate, 4-aminobutyric acid, beta-alanine, tyrosine, pyruvic acid and pantothenic acid were identified between 7-d samples and 21-d samples. The relative level of melibiose, ribose, maltotriose, D-glycerol-1-phosphate, tyrosine and pyruvic acid in samples collected on day 21 was significantly higher than that in samples collected on day 7(P < 0.01),respectively. Eight differential metabolites including ribose, sorbose, galactinol, cellobiose, pyruvic acid,galactonic acid, pantothenic acid and guanosine were found between 21-d samples and 35-d samples.Samples collected on day 35 had a higher relative level of ribose than that in samples collected on day 21(P < 0.01), In conclusion, many differential metabolites which have important effects on the growth and health of pigs are identified and findings contribute to explain the difference in feeding response of fermented liquid diet.

关键词： Bacillus subtilis Fermented liquid diet Differential metabolites GC-TOF-MS

妇女产后1年体重滞留列线图预测模型的构建与评估

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卫生研究 2024年第3期53卷 368-374,395页

作者：全春霞唐薇张喆庆毛丽梅南方医科大学公共卫生学院广州510515

目的分析妇女产后1年体重滞留的影响因素,构建并评估产后1年体重滞留的列线图预测模型。方法于2010年9月—2011年2月在广州市越秀区和白云区妇幼保健院招募468名孕晚期孕妇,追踪至产后1年,采用自制问卷收集孕妇的基本人口学信息,3天24... 详细信息

目的分析妇女产后1年体重滞留的影响因素,构建并评估产后1年体重滞留的列线图预测模型。方法于2010年9月—2011年2月在广州市越秀区和白云区妇幼保健院招募468名孕晚期孕妇,追踪至产后1年,采用自制问卷收集孕妇的基本人口学信息,3天24小时膳食回顾法调查孕晚期膳食摄入,测量妇女分娩前及产后1年体重。根据产后1年滞留体重是否>0 kg,将研究对象分为产后1年体重滞留组和体重恢复组,运用Logistic回归筛选产后1年体重滞留的影响因素。应用R 4.2.3软件构建列线图预测模型,并采用受试者工作特征曲线、校准曲线及Hosmer-Lemeshow拟合优度检验、临床决策曲线评估模型的区分度、准确度及临床适用性。结果模型训练集329名研究对象产后1年体重滞留率为68.09%,滞留体重的四分位数水平为5.0(3.0,10.0)kg。经Logistic回归分析,以孕前体质指数、孕期增重、产次、孕次、产后0~6月喂养方式构建列线图预测模型,模型区分度(AUC_(训练)=0.778,AUC_(验证)=0.767)和准确度(Hosmer-Lemeshow检验:P训练=0.946,P_(验证)=0.891)较好。结论基于妇女孕前体质指数、孕期增重、产次、孕次、产后0~6月喂养方式构建的产后1年体重滞留列线图模型具有良好的区分度、准确度和临床适用度。

关键词：体重滞留列线图产后

孕期盆底门诊干预在足月初产妇阴道分娩中的应用效果

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实用妇产科杂志 2024年第5期40卷 405-408页

作者：周隽毛胜艳林春霞何国琳苗娅莉唐佳成都市龙泉驿区第一人民医院(四川大学华西医院龙泉医院)产科四川成都610100 四川大学华西第二医院妇产科四川成都610000

目的:探讨孕期实施盆底门诊干预在足月初产妇阴道分娩中的应用效果。方法:将成都市龙泉驿区第一人民医院2021年1月至12月建卡有顺产意愿的单胎初产妇按照建档顺序单双数分组:单数组进行产科门诊常规管理及盆底门诊干预(凯格尔运动联合... 详细信息

目的:探讨孕期实施盆底门诊干预在足月初产妇阴道分娩中的应用效果。方法:将成都市龙泉驿区第一人民医院2021年1月至12月建卡有顺产意愿的单胎初产妇按照建档顺序单双数分组:单数组进行产科门诊常规管理及盆底门诊干预(凯格尔运动联合会阴按摩)200例(研究组),双数组进行常规产科门诊管理200例(对照组),比较两组阴道炎发生率、剖宫产率、阴道分娩率、产科裂伤情况、阴道助产率、会阴切开情况、分娩结局及分娩前及产后42天会阴体、生殖裂孔长度、阴道宽度及其他阴道分娩并发症(尿潴留和产褥期痔)等情况。结果:最终研究组纳入87例,对照组104例。研究组会阴切开率、会阴完整率、阴道助产率、会阴Ⅰ度和Ⅱ度裂伤率、产后出血量均优于对照组(P0.05)。结论:孕期实施盆底门诊干预可在一定程度上减轻足月初产妇经阴道分娩造成的伤害,并且未增加新生儿分娩并发症,值得临床推广。

关键词：盆底门诊足月初产妇阴道分娩凯格尔运动会阴按摩

Chinese Society of Cardiology guidelines on the diagnosis and treatment of adult fulminant myocarditis

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Science China(Life Sciences) 2024年第5期67卷 913-939页

作者： Chinese Society of Cardiology,Chinese Medical Association,Writing Group Jiangang Jiang Hongyang Shu Dao Wen Wang Rutai Hui Chenze Li Xiao Ran Hong Wang Jing Zhang Shaoping Nie Guanglin Cui Dingcheng Xiang Qun Shao Shengyong Xu Discussion experts:Ning Zhou Yuming Li Wei Gao Yuguo Chen Yuan Bian Guoping Wang Liming Xia Yan Wang chunxia Zhao Zhiren Zhang Yuhua Zhao Jianan Wang Shaoliang Chen Hong Jiang Jing Chen Xianjin Du mao Chen Yinxian Sun Sheng Li Hu Ding Xueping Ma Hesong Zeng Li Lin Shenghua Zhou Likun Ma Ling Tao Juan Chen Yiwu Zhou Xiaomei Guo 不详 Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Fuwai Hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Zhongnan Hospital of Wuhan University Wuhan 430062China Fuwai Huazhong Cardiovascular Hospital Zhengzhou 450003China Beijing Anzhen Hospital Capital Medical UniversityBeijing 100029China Guangzhou General Hospital of Guangzhou Military Command Guangzhou 510010China Harbin Medical University Cancer Hospital Harbin 150081China Union Hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Taida Hospital Tianjin 300457China Peking University Third Hospital Beijing 100191China Qilu Hospital of Shandong University Jinan 250012China Kanghua Hospital DongguanGuangzhou 523080China Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003China Nanjing First Hospital Nanjing Medical UniversityNanjing 210006China Renmin Hospital of Wuhan University Wuhan 430060Wuhan West China Hospital Sichuan UniversityChengdu 610044China First Hospital of China Medical University Shenyang 110002China General Hospital of Ningxia Medical University Yinchuan 750003China The Second Xiangya Hospital Central South UniversityChangsha 410012China The First Affiliated Hospital of USTC University of Science and Technology of ChinaHefei 230002China The First Affiliated Hospital of Air Force Medical University Xi’an 710032China Central Hospital of Wuhan City Wuhan 430014China Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

Fulminant myocarditis is an acute diffuse inflammatory disease of myocardium.It is characterized by acute onset,rapid progress and high risk of death.Its pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory storm.According to China’s practical experience,the adoption of the“life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core)can significantly improve the survival rate and long-term prognosis.Special emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.

关键词： fulminant myocarditis guidelines life support-based comprehensive treatment regimen diagnosis treatment cytokine storm overactivation of immunity

从“效应-靶标”互作关联视角探讨消肿止痛贴的功效机制

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中国实验方剂学杂志 2024年第13期30卷 106-113页

作者：毛鹏李玮婕李春霞马旺明张彦琼许海玉陕西摩美得气血和制药有限公司陕西咸阳712000 中国中医科学院中药研究所北京100700 国家药品监督管理局中医药研究与评价重点实验室北京100700

目的:从“效应-靶标”互作关联视角探讨消肿止痛贴的功效机理。方法:基于中国学术期刊全文数据库(CNKI)和PubMed数据库收集消肿止痛贴组方药材沙蒿子、水蔓菁、薄荷脑中所含的化学成分信息,利用中医药百科全书(ETCM 2.0,http://***/ETCM... 详细信息

目的:从“效应-靶标”互作关联视角探讨消肿止痛贴的功效机理。方法:基于中国学术期刊全文数据库(CNKI)和PubMed数据库收集消肿止痛贴组方药材沙蒿子、水蔓菁、薄荷脑中所含的化学成分信息,利用中医药百科全书(ETCM 2.0,http://***/ETCM2/front/#/)收集其透皮吸收特性,筛选Golden Triangle为Accepted的成分预测候选靶标谱预测。利用中医药整合药理学研究平台(TCMIP v2.0),依据化合物结构相似性原理,预测其候选靶标谱。同时,利用SoFDA数据平台收集消肿止痛贴功效相关的症状与其相关基因信息。进一步,利用上述方药候选靶标与其功效相关基因之间的相互作用信息,构建消肿止痛贴的“效应-靶标”互作关联网络,通过计算网络节点拓扑特征值,筛选网络核心靶标,并开展功能挖掘,阐释消肿止痛贴的功效机制。结果:基于ETCM 2.0及TCMIP v2.0数据库共得到165个方药候选靶标,基于SoFDA数据库收集到清热、消肿、止痛功效相关症状及其1744个相关基因。网络构建与分析发现,消肿止痛贴的核心效应靶标主要参与调节机体的“免疫-炎症”平衡、维持物质与能量代谢稳态、血液循环和神经系统功能,与该方清热、消肿、止痛功效紧密关联。其中,消肿止痛贴-清热功效组具有清热解毒、抗菌消炎之功,其核心效应靶标群的生物学功能与矫正病原体诱导机体“免疫-炎症”失衡有关;消肿止痛贴-消肿功效组具有利水消肿、软坚散结之功,其核心效应靶标群的生物学功能与改善微循环障碍有关;消肿止痛贴-止痛功效组具有化瘀解滞、活血止痛之功,其核心效应靶标群与纠正神经系统和物质及能量代谢紊乱有关。结论:消肿止痛贴清热、消肿和止痛的功效可能与其作用于相关候选靶标,从而矫正“神经-免疫-血管轴”失衡,调节神经元兴奋性和炎症反应,干预物质和能量代谢密切相关。相关研究结果为明确消肿止痛贴的优势疗效环节和辅助其临床精准定位奠定理论基础。

关键词： “效应-靶标”关联网络消肿止痛贴清热消肿止痛

环泊酚复合地佐辛用于人工流产术麻醉半数有效剂量的测定

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河南医学研究 2024年第9期33卷 1566-1570页

作者：茅晓玉李梦倩苏春侠郑州大学第二附属医院麻醉与围术期医学部河南郑州450014

目的测定环泊酚复合地佐辛用于人工流产术的半数有效剂量。方法将2023年11月于郑州大学第二附属医院拟行无痛人工流产术的25例患者纳入研究,体重指数为18~25 kg·m^(-2),采用静脉全身麻醉。静脉注射地佐辛2.5 mg后,根据序贯法给予环泊酚... 详细信息

目的测定环泊酚复合地佐辛用于人工流产术的半数有效剂量。方法将2023年11月于郑州大学第二附属医院拟行无痛人工流产术的25例患者纳入研究,体重指数为18~25 kg·m^(-2),采用静脉全身麻醉。静脉注射地佐辛2.5 mg后,根据序贯法给予环泊酚,初始剂量0.33 mg·kg^(-1),浓度梯度为0.02 mg·kg^(-1),麻醉后患者眼睑反射消失且宫腔操作5 min内无体动反应,判定为有效(阴性),反之即为无效(阳性),如为阴性环泊酚降低1个浓度梯度,反之升高1个浓度梯度。用Probit概率回归法计算半数有效剂量。结果25例患者中12例阴性,13例阳性。环泊酚复合地佐辛用于无痛人流患者全麻诱导时环泊酚的半数有效剂量为0.346 mg·kg^(-1),95%置信区间为0.322~0.376 mg·kg^(-1)。结论环泊酚复合地佐辛用于无痛人流术患者环泊酚的半数有效剂量为0.346 mg·kg^(-1)。

关键词：环泊酚注射液地佐辛无痛人流术半数有效剂量

维普期刊数据库博看期刊

iCubate-iC血培养快速检测系统与血培养传统鉴定药敏一致性评估

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分子诊断与治疗杂志 2024年第5期16卷 949-952,957页

作者：刘振嘉范欣毛熠张丹杨春霞首都医科大学附属北京朝阳医院-北京市呼吸疾病研究所感染和临床微生物科北京100020

目的评估基于PCR扩增荧光探针捕获技术的iCubate-iC血培养快速检测系统与血培养传统鉴定药敏结果的一致性。方法本次评估入组了首都医科大学附属北京朝阳医院2017年11月26日至2018年4月18日的121例临床血液细菌培养阳性标本。以临床常... 详细信息

目的评估基于PCR扩增荧光探针捕获技术的iCubate-iC血培养快速检测系统与血培养传统鉴定药敏结果的一致性。方法本次评估入组了首都医科大学附属北京朝阳医院2017年11月26日至2018年4月18日的121例临床血液细菌培养阳性标本。以临床常规培养鉴定结果及体外药敏结果为参考方法,验证iCubate-iC系统鉴定结果及耐药位点检出的一致性。结果对比MALDI-TOF质谱鉴定结果,iCubate-iC系统鉴定符合率达到95.9%(116/121)。有4例假阴性结果,所有靶标显示“未检出”。iCubate-iC系统成功检出1例金黄色葡萄球菌和表皮葡萄球菌的混合样本,而1例大肠埃希菌和铜绿假单胞菌复合样本漏检了铜绿假单胞菌。iC-GPC检出的31例葡萄球菌中,有18例检出了mecA基因,其中有1例表皮葡萄球菌检出mecA,但其体外药敏结果显示对头孢西丁敏感。肠球菌中有1例检出vanA基因,但万古霉素体外药敏检测敏感。CTX-M group1基因检测与β内酰胺类药物的吻合性不理想,但革兰氏阴性菌碳青霉烯酶基因(KPC、NDM)检测结果与碳青霉烯类药物的药敏结果能够完全吻合。结论iCubate-iC检测系统对于临床常见引起血流感染细菌的检测结果可靠,且具有快速简便的优点,为引起血流感染细菌的快速诊断提供了有力工具。

关键词：血流感染血培养快速鉴定耐药基因

Chinese Society of Cardiology guidelines on the diagnosis and treatment of adult fulminant myocarditis

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Science China Life Sciences 2024年

作者： Jiangang Jiang Hongyang Shu Dao Wen Wang Rutai Hui Chenze Li Xiao Ran Hong Wang Jing Zhang Shaoping Nie Guanglin Cui Dingcheng Xiang Qun Shao Shengyong Xu Ning Zhou Yuming Li Wei Gao Yuguo Chen Yuan Bian Guoping Wang Liming Xia Yan Wang chunxia Zhao Zhiren Zhang Yuhua Zhao Jianan Wang Shaoliang Chen Hong Jiang Jing Chen Xianjin Du mao Chen Yinxian Sun Sheng Li Hu Ding Xueping Ma Hesong Zeng Li Lin Shenghua Zhou Likun Ma Ling Tao Juan Chen Yiwu Zhou Xiaomei Guo Peking University Third Hospital Qilu Hospital of Shandong University The First Affiliated Hospital of Air Force Medical University Zhongnan Hospital of Wuhan University First Hospital of China Medical University General Hospital of Ningxia Medical University The First Affiliated Hospital of USTC University of Science and Technology of China Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing Anzhen Hospital Capital Medical University Chinese Medical Association Harbin Medical University Cancer Hospital Union Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Renmin Hospital of Wuhan University Central Hospital of Wuhan City Taida Hospital Guangzhou General Hospital of Guangzhou Military Command West China Hospital Sichuan University Nanjing First Hospital Nanjing Medical University Chinese Society of Cardiology Kanghua Hospital Dongguan Fuwai Huazhong Cardiovascular Hospital Tongji Hospital Tongji Medical College Huazhong University of Science and Technology The Second Xiangya Hospital Central South University Second Affiliated Hospital Zhejiang University School of Medicine

Fulminant myocarditis is an acute diffuse inflammatory disease of myocardium. It is characterized by acute onset, rapid progress and high risk of death. Its pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory storm.According to China’s practical experience, the adoption of the “life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core) can significantly improve the survival rate and long-term prognosis. Special emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.

关键词：

木质素磺酸催化Biginelli反应合成3,4-二氢嘧啶-2(1H)-酮化合物

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化学与粘合 2024年第2期46卷 107-112,116页

作者：刘瑞岐胡旭颖毛渊渊陈春霞彭进松东北林业大学化学化工与资源利用学院黑龙江哈尔滨150040

为了绿色、高效地制备3,4-二氢嘧啶-2-酮衍生物,在无溶剂70℃条件下,以木质素磺酸为催化剂,单或多取代苯甲醛、尿素或硫脲与乙酰乙酸乙酯为原料,通过Biginelli反应“一锅法”合成了一系列3,4-二氢嘧啶-2(1H)-酮产物。产物结构经~1H和^(1... 详细信息

为了绿色、高效地制备3,4-二氢嘧啶-2-酮衍生物,在无溶剂70℃条件下,以木质素磺酸为催化剂,单或多取代苯甲醛、尿素或硫脲与乙酰乙酸乙酯为原料,通过Biginelli反应“一锅法”合成了一系列3,4-二氢嘧啶-2(1H)-酮产物。产物结构经~1H和^(13)C NMR表征。研究结果表明:与经典的Biginelli反应条件相比,该催化体系对官能团普适性良好、产率优良,最高产率可达94%。该方法是一种具有绿色、温和、合成操作简单、产物纯化后处理方便等优点的制备3,4-二氢嘧啶-2-酮衍生物的新方法。

关键词：木质素磺酸 Biginelli反应无溶剂合成 3,4-二氢嘧啶-2(1H)-酮