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Synergistic precipitant powered self-assembly of papain for cross-linked enzyme crystals preparation

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Particuology 2024年第2期85卷 102-112页

作者： Qi Hao Pan Guo Honghai Wang Min Su chunli li Yuqi Hu Weiyi Su School of Chemical Engineering and Technology Hebei University of TechnologyTianjin300130China National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization Tianjin300130China

In this study,we prepared cross-linked enzyme crystals(CLECs)of papain to further broaden the application of the enzyme with high activity in extreme ***,papain crystals were successfully obtained based on the micro-batch,batch,and expanded batch crystallization ***,ammonium sulfate and polyethylene glycol 6000(PEG6000)were synergistically used as the precipitants,while L-cysteine was applied to enhance the activity of ***,the interaction between L-cysteine and papain was modeled by molecular docking *** was found that L-cysteine could form a hydrogen bond with aspartic acid residue(Asp)at site 158,and the electrostatic attraction with lysine residue(Lys)at site 156 was also quite *** the enzyme crystals were cross-linked by glutaraldehyde at optimized *** papain CLECs were identified by various methods,and it was found that the thermal stability and enzymatic activity both increased compared to the raw *** importantly,it could be applied at more rigorous conditions,for example,pH of 4.

关键词： Papain Cross-linked enzyme crystals L-cysteine Molecular docking Crystallization

Stable Cycling of All-Solid-State lithium Metal Batteries Enabled by Salt Engineering of PEO-Based Polymer Electrolytes

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Energy & Environmental Materials 2024年第2期7卷 67-74页

作者： Lujuan liu Tong Wang li Sun Tinglu Song Hao Yan chunli li Daobin Mu Jincheng Zheng Yang Dai Department of Chemical Engineering Shanghai UniversityShangda Road 99Shanghai 200444China School of Material Science&Engineering Beijing Key Laboratory of Environmental Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Department of Physics Xiamen UniversityXiamen 361005China

Poly(ethylene oxide)(PEO)-based polymer electrolytes show the prospect in all-solid-state lithium metal batteries;however,they present limitations of low room-temperature ionic conductivity,and interfacial incompatibility with high voltage ***,a salt engineering of 1,1,2,2,3,3-hexafluoropropane-1,3-disulfonimide lithium salt(liHFDF)/liTFSI system was developed in PEO-based electrolyte,demonstrating to effectively regulate li ion transport and improve the interfacial stability under high *** show,by manipulating the interaction between PEO matrix and TFSI^(-)-HFDF^(-),the optimized solid-state polymer electrolyte achieves maximum li+conduction of 1.24×10^(-4)S cm^(-1)at 40℃,which is almost 3 times of the ***,the optimized polymer electrolyte demonstrates outstanding stable cycling in the liFePO_(4)/li and liNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)/li(3.0-4.4 V,200 cycles)based all-solid-state lithium batteries at 40℃.

关键词： all-solid-state battery high voltage li-ion conductivity molecular interaction poly(ethylene oxide)

Co-inoculation of Streptomyces salinarius and Bacillus halodurans Enhances the Biocontrol Effects Against Damping off of Pinus by Manipulating Rhizosphere Microbial Community

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Co-inoculation of Streptomyces salinarius and Bacillus halod...

中国植物病理学会2024年学术年会

作者： WANG Haixia li chunli WANG Siyang FU lin ZHU Chunyu School of life Sciences Liaoning University

Damping-off is a worldwide disease in forest nurseries caused by Fusarium *** genera of Bacillus and Streptomyces are popularly used as the biological control agents for damping-off diseases ***,there are few reports involving the combined use of these two *** study aimed to screen the compatible interactive Bacillus *** Streptomyces *** control damping-off of pine seedlings,and to investigate the colonization ability of strains in plant rhizosphere and the effect of single and co-inoculation on soil microbiome by fluorescence quantitative PCR and high-throughput sequencing,*** vitro experiments showed that Streptomyces salinarius Lnu-12 and Bacillus halodurans Lnu-20 strongly inhibit the growth of *** and they are compatible with each *** biofilm and motility assays,the results suggested that the biofilm of Lnu-20 was significantly increased when co-cultured with Lnu-12,while Lnu-20 contributed to the migration of *** pot experiment indicated that co-inoculation of Lnu-12 and Lnu-20(12+20)displayed an improved control efficacy against damping off of pine seedlings compared with the single-inoculation of Lnu-12(12) and Lnu-20(20),as well as significantly promoted the growth of pine *** abundance of Lnu-12 and Lnu-20 was obviously higher and the abundance of *** was significantly reduced in the 12+20 treatment than 12 and 20 treatment in pine seedlings rhizosphere soil,***,co-inoculated treatment altered the composition and structure of the microbial community and enriched beneficial bacteria in rhizosphere soil compared with the single-inoculated *** conclusion,co-inoculation of *** Lnu-12 and *** Lnu-20 could effectively control the damping-off disease and promoted the growth of pine seedlings by colonizing the plant rhizosphere and manipulating microbial *** may provide theoretical guidance and technical support for t

关键词： pine seedlings damping-off co-inoculation microbial community

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污泥陶粒的功能化改性及含磷废水处理研究

功能材料 2024年第4期55卷 4142-4151页

作者：郝禅光李春立李权邢丽静梁欢徐峥武汉工程大学资源与安全工程学院武汉430074 长江生态环保集团有限公司武汉430077

随着城市规模的高速发展,市政污水的处理量陡增,导致污泥固废的处置问题日益显著。以污泥为主要原料,添加粉煤灰、蒙脱石为辅料制备多孔陶粒(SC),采用共沉淀法进行功能化改性,制备得到镧改性污泥陶粒(L-SC)和镧铁改性污泥陶粒(LF-SC)。... 详细信息

随着城市规模的高速发展,市政污水的处理量陡增,导致污泥固废的处置问题日益显著。以污泥为主要原料,添加粉煤灰、蒙脱石为辅料制备多孔陶粒(SC),采用共沉淀法进行功能化改性,制备得到镧改性污泥陶粒(L-SC)和镧铁改性污泥陶粒(LF-SC)。以pH值、投加量以及干扰阴离子为实验因素考察对模拟含磷废水中磷吸附的性能,通过分析表征讨论对磷的吸附机理。结果表明,L-SC和LF-SC具有致密的孔结构,S_(BET)分别为17 148.7和17 439.2 cm^(2)·g^(-1),改性陶粒的吸附性能明显优于SC,在pH=5时L-SC对磷的去除率最高可达93.74%,而LF-SC对磷的去除率更高且吸附效果受pH影响较小,在5≤pH≤9时磷去除率均在97%以上,拟二级动力学模型和Freundlich模型能较好地描述改性陶粒对磷的吸附。FTIR和SEM-EDS结果显示,过渡金属Fe对稀土金属La具有明显的活化作用,极大提高了陶粒的吸附性能,吸附磷酸盐的主要机制为静电引力和配体交换形成稳定络合物。

关键词：污泥陶粒改性除磷

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磷酸在GO插层阶段的功能化调控及机理

高等学校化学学报 2024年第1期45卷 61-71页

作者：王佳瑞李春丽程佳豪郝亚玲周楠杨鹏内蒙古工业大学资源与环境工程学院环境污染控制与修复自治区高等学校重点实验室呼和浩特010000

针对氧化石墨烯(GO)制备过程中插层阶段的调控及机理研究对GO功能化应用于电极材料具有重要的研究意义.本文在改进Hummers法的基础上,向H_(2)SO_(4)插层剂中加入不同体积的H_(3)PO_(4),制备了不同氧化程度的GO.利用扫描电子显微镜(SEM)... 详细信息

针对氧化石墨烯(GO)制备过程中插层阶段的调控及机理研究对GO功能化应用于电极材料具有重要的研究意义.本文在改进Hummers法的基础上,向H_(2)SO_(4)插层剂中加入不同体积的H_(3)PO_(4),制备了不同氧化程度的GO.利用扫描电子显微镜(SEM)、X射线光电子能谱(XPS)和傅里叶变换红外光谱(FTIR)等表征手段,分析了不同氧化程度的GO的微观形貌、元素组成、氧化程度,以探究H_(3)PO_(4)在插层石墨过程中的作用机理;并采用循环伏安法(CV)和交流阻抗法(EIS)对不同H_(2)SO_(4)/H_(3)PO_(4)体积比下的GO进行电化学性能测试,分析了H_(3)PO_(4)对GO电化学性能的影响,以达到调控石墨的插层氧化从而提升GO导电性的目的.结果表明,单一的H_(2)SO_(4)使GO基面上的邻位二醇过度氧化造成孔洞,H_(3)PO_(4)的加入会扩大石墨层间距,使氧化剂更易进入石墨层间,并与1,2-二醇反应生成环状结构以起到保护基面的作用,从而提高GO的导电性.H_(3)PO_(4)作为辅助酸会协助H_(2)SO_(4)制备基面更加完整且氧化程度更高的GO,但其酸性较弱,不可完全代替H_(2)SO_(4)在氧化过程中的作用.

关键词：氧化石墨烯磷酸插层机理研究电化学化学反应部分氧化

后疫情时代居民焦虑、抑郁和失眠现状及疫情相关影响因素

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中华疾病控制杂志 2024年第6期28卷 709-713,735页

作者：刘小畅程桂荣李春利曾燕武汉科技大学医学院脑科学先进技术研究院武汉430081 武汉科技大学公共卫生学院流行病与卫生统计学系武汉430065

目的探究后疫情时代居民焦虑、抑郁和失眠等心理问题的现状及疫情相关因素对心理问题的影响。方法于2023年1―4月,通过在线横断面调查,对焦虑、抑郁和失眠进行标准化测量以及与不良心理健康结果相关的风险因素进行调查;心理评估量表通... 详细信息

目的探究后疫情时代居民焦虑、抑郁和失眠等心理问题的现状及疫情相关因素对心理问题的影响。方法于2023年1―4月,通过在线横断面调查,对焦虑、抑郁和失眠进行标准化测量以及与不良心理健康结果相关的风险因素进行调查;心理评估量表通过采用t检验、方差分析和χ^(2)检验的方法比较各组焦虑、抑郁和失眠的差异,Pearson相关分析用于探究后疫情时代相关因素与焦虑、抑郁和睡眠质量评分的相关性,线性回归模型确定后疫情时代相关因素与焦虑、抑郁和睡眠质量的定量关系。结果研究共纳入35213名≥16岁的居民,患焦虑症共8464人(24.0%),广泛性焦虑障碍(generalized anxiety disorder-7,GAD-7)评分为(6.30±5.06)分,患抑郁症共12216人(34.7%),抑郁症筛查量表(patient health questionnaire-9,PHQ-9)评分为(7.98±6.12)分,患失眠症共12416人(35.3%),匹兹堡睡眠质量指数(Pittsburgh sleep quality index,PSQI)评分为(8.75±3.94)分。焦虑、抑郁和睡眠质量得分均与疫情感染指数(infection index,INI)呈负相关(β=-0.083,-0.042,-0.069,均P<0.001),焦虑、抑郁和睡眠质量得分均与疫情影响指数(impact index,IMI)和疫情忧虑指数(concern index,COI)呈正相关(β=0.401,0.365,0.207,均P<0.001)。结论后疫情时代,居民焦虑、抑郁及失眠症状检出率较高,从改善疫情导致的生活、社交不便和担忧出发,帮助人们以良好的心理健康水平应对新型冠状病毒感染(corona virus disease 2019,COVID-19)的长期影响。

关键词：新型冠状病毒感染焦虑抑郁睡眠质量影响因素

青藏高原高寒草甸微地形对土壤胞外酶活性及生态化学计量比的影响

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应用与环境生物学报 2024年第2期30卷 212-219页

作者：李欣蔚肖元明李春丽高永恒周国英青海大学生态环境工程学院西宁810016 青海大学省部共建三江源生态与高原农牧业国家重点实验室西宁810016 中国科学院西北高原生物研究所西宁810008 中国科学院藏药研究重点实验室西宁810008 中国科学院成都生物研究所成都610213

高寒草甸的生态化学计量特征是当前生态学研究的热点问题之一.以青藏高原高寒草甸为研究对象,测定3种微地形(阴坡、阳坡和沟底)的土壤养分含量以及与碳、氮、磷循环相关的酶活性;同时,调查植物群落特征、土壤pH和含水量(SWC),计算植物... 详细信息

高寒草甸的生态化学计量特征是当前生态学研究的热点问题之一.以青藏高原高寒草甸为研究对象,测定3种微地形(阴坡、阳坡和沟底)的土壤养分含量以及与碳、氮、磷循环相关的酶活性;同时,调查植物群落特征、土壤pH和含水量(SWC),计算植物物种多样性指数、土壤关键元素及相应胞外酶的生态化学计量比,探究小尺度高寒草甸上微地形对土壤关键元素、胞外酶活性及相应生态化学计量比的影响因素.结果表明:(1)不同微地形条件下,植物群落组成和多样性差异极显著,阳坡植物多样性最高,阴坡最低(P<0.001).(2)在测定的所有指标中,土壤pH和酸性磷酸酶(AP)在阴坡最高,阳坡的土壤总磷(TP)含量最高,其余指标无显著变化.(3)微地形造成的植物多样性的改变是影响土壤C:N的重要原因,物种多样性与土壤C:N存在极显著的负相关性(P<0.01).(4)地下生物量与土壤C:N具有显著的正相关关系(P<0.05),说明生物量差异是主导土壤C:N变化的关键因素之一.(5)植被因素、土壤pH和土壤TP含量共同调控土壤关键元素和相应胞外酶的生态化学计量在微地形中的变化.本研究表明微地形导致的植物群落特征和土壤理化性质的差异对胞外酶活性及生态化学计量比变化具有重要影响,结果可为厘清未来气候变化背景下青藏高原高寒草甸关键元素循环过程提供重要的理论支持.(图4表5参50)

关键词：土壤酶活生态化学计量微地形高寒草甸物种多样性

Magic of hydrogen spillover: Understanding and application

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Green Energy & Environment 2022年第6期7卷 1161-1198页

作者： Haifang Shen Hao li Zhensheng Yang chunli li National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization Tianjin Key Laboratory of Chemical Process SafetySchool of Chemical Engineering and TechnologyHebei University of Technology8 Guangrong RoadTianjin300130China

The hydrogen spillover effect(HSPE)plays an important role in heterogeneous catalysis and hydrogen storage as an interfacial phenomenon,which facilitates the improvement of hydrogen storage properties of porous nanomaterials and indirectly or directly affects the reaction performance of multiphase catalytic *** setting-up of the word“hydrogen spillover”opened up a new area to gain insight into the dynamic behavior of migrating hydrogen atoms on a catalyst ***,a comprehensive understanding of the HSPE is still ***,the development of advanced characterization techniques provides increasingly valuable information to further our understanding of the *** on these considerations,in this review,we hope to provide some answers to the question“What is hydrogen spillover and how do we recognize it?”.To do this,we will rely on advanced characterization techniques as well as experimental and theoretical ***,we discuss in detail the influences of the HSPE on hydrogen storage performance and the important catalytic effects of the HSPE in *** effects will be reviewed by looking through the catalytic results obtained in many reactions in thermal catalysis,electrocatalysis,and ***,based on the application potential of hydrogen spillover,we present some preliminary research proposals and discuss the opportunities and challenges that remain to be faced in this research area.

关键词： Hydrogen spillover Characterization Hydrogen storage Catalysis Spillover mechanism

Process intensification and energy saving of reactive distillation for production of ester compounds

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Chinese Journal of Chemical Engineering 2019年第6期27卷 1307-1323页

作者： chunli li Cong Duan Jing Fang Hongshi li School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization Tianjin 300130China

Reactive distillation(RD) process is an innovative hybrid process combining reaction with distillation, which has recently come into sharp focus as a successful case of process intensification. Considered as the most representative case of process intensification, it has been applied for many productions, especially for production of ester compounds. However, such problems existing in the RD system for ester productions are still hard to solve,as the removal of the water which comes from the esterification, and the separation of the azeotropes of ester–alcohol(–water). Many methods have been studying on the process to solve the problems resulting in further intensification and energy saving. In this paper, azeotropic–reactive distillation or entrainer enhanced reactive distillation(ERD) process, reactive extractive distillation(RED) process, the method of co-production in RD process, pressure-swing reactive distillation(PSRD) process, reactive distillation–pervaporation coupled process(RD–PV), are introduced to solve the problems above, so the product(s) can be separated efficiently and the chemical equilibrium can be shifted. Dividing-wall column(DWC) structure and novel methods of loading catalyst are also introduced as the measures to intensify the process and save energy.

关键词： Reactive distillation Process intensification Process coupling Thermal coupling