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Chemical, carbon and sulfur isotopic compositions constrain the origin of Upper Carboniferous-Lower Triassic gases in eastern Sichuan Basin, SW China

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Science China Earth Sciences 2024年第10期67卷 3169-3185页

作者： chunfang cai Ilya KUTUZOV Wenhua MEI Daowei WANG Bing LUO Shipeng HUANG Bing HE Alon AMRANI China University of Petroleum National Key Lab Petroleum Resource&EngineeringBeijing 102249China State Key Laboratory of Lithospheric and Environmental Coevolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Institute of Earth Sciences The Hebrew UniversityJerusalem 91904Israel College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China Yulin University Yulin 719000China Research Institute of Exploration and Development Southwest Oil&Gas Field CompanyPetroChinaChengdu 610051China PetroChina Research Institute of Petroleum Exploration and DevelopmentBeijing 100083China

Methane dominated gas is one of the cleanest energy resources;however, there is no direct method to determine its source rock. Natural gases produced from the eastern Sichuan Basin together with seismic data were studied for their sources and secondary alteration by thermochemical sulfate reduction(TSR). Our results demonstrate that Upper Permian to Lower Triassic(P_(3)ch-T_(1)f) gases in the surrounding of the Kaijiang-Liangping area show volatile organic sulfur compounds(VOSCs) δ^(34)S values close to those of the associated H_(2)S, and may have been altered by methane-dominated TSR, resulting in positive shift in methane δ^(13)C_(1)values with increasing TSR extents. Other(or group 2) gases produced from the P_(3)ch-T_(1)f reservoirs from the southern area and the Upper Carboniferous to Middle Permian(C_(2)h-P_(2)q) from the eastern Sichuan Basin are not significantly changed by TSR, show similar δ^(34)S values between the kerogens and some VOSCs, and may have been derived from the Lower Silurian and Middle Permian source rocks. This study demonstrates a case for the first time showing the δ^(34)S values of VOSCs can be used as a tool for direct correlation between non-TSR altered gas and source rocks. Methane-dominated gas pools can be found using gas and source rock geochemistry combined with seismic data.

关键词： Sulfur isotopes Volatile organic sulfur compounds Thermochemical sulfate reduction Sichuan Basin Gas-source rock correlation

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Filamentous cyanobacteria fossils and their signifi-cance in the Permian-Triassic boundary section at Laolongdong,Chongqing

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Chinese Science Bulletin 2008年第12期53卷 1871-1879页

作者： JIANG HongXia WU YaSheng cai chunfang Key Laboratory of Mineral Resources Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

The microbial communities blooming immediately after the end-Permian mass extinction represent abnormally extreme environments, and vary in different areas. In this study, filamentous cyanobacterial biota was found in the strata after the extinction in the famous Permian-Triassic boundary section at Laolongdong, Chongqing, southwest China. In thin sections, the filamentous cyanobacterial fossils are below 1 mm in length, and generally taper to one end, with the widest diameter up to 0.08 mm. Some of them are curved, indicating that they are soft in life. Their walls are composed of cryptocrystalline to microcrystalline calcites. The filaments have round cross section, and are internally filled with micrites and fine sparry calcites, which indicate that the filaments are originally empty. They are randomly dis-tributed in the rocks, but in some places, they tend to be distributed in radial pattern. The filamentous organisms are morphologically similar to Rivularia of Rivulariaceae, Cyanobacteria Phylum, but with calcified sheaths, and are tentatively regarded as an indeterminate new species in Rivularia: Rivularia sp. Cyanobacteria are photosynthetic autotrophic, and can survive in dysoxic condition. The blooming of this organism and the absence of other organisms may indicate that the environment was oxygen- deficient and shallow, since this photosynthetic autotrophic organism needed to live within photic zone.

关键词：蓝藻化石植物二叠纪古生物学

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饲料酵母水溶物具有促进草鱼原代肝细胞生长及修复过氧化氢损伤的作用

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中国粮油学报 2024年第1期39卷 159-166页

作者：马杰王卓君石瑶瑶叶元土蔡春芳吴萍陈鹏苏州大学基础医学与生物科学学院苏州215123 江苏省水产动物营养重点实验室苏州215123 北京英惠尔生物技术有限公司北京100081

研究以3种酵母水溶物为实验材料,探究饲料酵母对草鱼原代肝细胞生长的促进作用和过氧化氢(H_(2)O_(2))诱导草鱼原代肝细胞氧化损伤的缓解作用。实验使用酶消化法分离得到草鱼原代肝细胞,以200μmol/L H_(2)O_(2)处理细胞1 h建立氧化应... 详细信息

研究以3种酵母水溶物为实验材料,探究饲料酵母对草鱼原代肝细胞生长的促进作用和过氧化氢(H_(2)O_(2))诱导草鱼原代肝细胞氧化损伤的缓解作用。实验使用酶消化法分离得到草鱼原代肝细胞,以200μmol/L H_(2)O_(2)处理细胞1 h建立氧化应激模型;分别选取质量浓度为25、50、100、200、400 mg/L的饲料酵母水溶物处理细胞,比较3种饲料酵母水溶物对原代肝细胞的促生长效果;分别使用3种饲料酵母的最佳促生长质量浓度处理模型细胞,分析饲料酵母水溶物对H_(2)O_(2)诱导的氧化损伤的缓解作用。结果表明:3种饲料酵母水溶物均能促进原代肝细胞增殖,酵母培养物YC-A、YC-B、酵母细胞壁多糖YCP的最佳促生长质量浓度分别为100、200、100 mg/L,在该质量浓度下细胞活力较对照组极显著提高(P<0.001)。相比于H_(2)O_(2)模型组,3个饲料酵母水溶物组细胞活力和MMP极显著提高(P<0.001),培养液中LDH活性极显著降低(P<0.001),细胞凋亡率和胞内ROS水平极显著降低(P<0.01);电镜结果显示,模型组细胞线粒体肿胀、嵴扭曲或消失,细胞内出现大量空泡,而3个饲料酵母水溶物处理组观察到线粒体肿胀情况得以改善。转录组结果提示,YCP可能通过增加细胞内Ⅰ相和Ⅱ相代谢酶保护细胞免受氧化损伤,而YC对细胞的保护可能是通过调控细胞内铁稳态实现的。饲料酵母水溶物能够促进草鱼原代肝细胞生长,并对原代肝细胞H_(2)O_(2)氧化损伤具有一定的修复作用。

关键词：饲料酵母原代肝细胞过氧化氢氧化损伤草鱼

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膳食纤维过量摄入对鱼类的致病作用和机制

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水产学报 2024年第4期48卷 241-256页

作者：蔡春芳任胜杰万金娟姚仕彬吴萍曹霞敏叶元土苏州大学基础医学与生物科学学院江苏省水产动物营养重点实验室江苏省淡水水产研究所江苏省现代农业(大宗鱼类)产业技术体系

由于环境保护和成本控制的需要，植物性饲料原料在水产配合饲料中的用量越来越高。植物性原料中含有丰富的膳食纤维(DFs)，使许多商品饲料中的DFs达30%以上，但由此带来的对水产动物的生理影响仍鲜受关注。本文介绍了DFs的定义、分类、... 详细信息

由于环境保护和成本控制的需要，植物性饲料原料在水产配合饲料中的用量越来越高。植物性原料中含有丰富的膳食纤维(DFs)，使许多商品饲料中的DFs达30%以上，但由此带来的对水产动物的生理影响仍鲜受关注。本文介绍了DFs的定义、分类、理化特性、可发酵性及常见植物性饲料原料中DFs的含量，重点介绍了作者及团队在鱼类DFs营养生理方面的研究发现。研究表明，黄颡鱼摄食含20%～30%DFs的饲料后会出现出血、白便、肠炎、腐皮、烂鳃、肝脂肪变性和纤维化、绿肝、白肝、胆囊肿大等症状及暴发性死亡。大口黑鲈和草鱼摄食高DFs饲料后也有相似症状。DFs的致病作用不仅与饲料中DFs含量有关，还与DFs种类有关，果胶对黄颡鱼的致病性比纤维素强得多。DFs的致病机制与其对胆汁酸(BAs)稳态和肠道微生物稳态的干扰有关。因DFs具有结合BAs的特性，这会阻碍BAs对法尼醇X受体(FXR)等BAs受体的激活，而FXR负反馈调控BAs的合成并抑制炎症反应，因此，大量DFs进入肠道后就会引起BAs合成亢进，提高组织中BAs水平及其疏水性并干扰BAs循环节律，再由BAs的细胞毒性引起炎症反应和组织坏死。DFs还具有可发酵性，引起肠道微生物结构改变，或由BAs的抗菌作用引起肠道微生物结构改变，进而加剧包括BAs在内的代谢紊乱。DFs的致病性与其他胁迫作用具有叠加效应，加重疾病症状和进展。从DFs的致病机制出发，通过控制DFs摄入种类和摄入量、向饲料中添加BAs及牛磺酸、防止DFs与其他胁迫因子的叠加效应等方式可防控DFs诱导的疾病。本研究首次阐述了DFs过量摄入对鱼类的致病性及其潜在机制，为植物性饲料原料的高值化利用及饲料配制技术优化提供了新视角，为鱼类病害防控提供了新途径。研究结果也提示未来应进一步阐明不同植物性原料中DFs的种类、含量和特性以及水产动物对DFs的耐受范围。

关键词：鱼类膳食纤维非感染性炎症胆汁酸法尼醇X受体肠道微生物

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B, Sr, O and H Isotopic Compositions of Formation Waters from the Bachu Bulge in the Tarim Basin

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Acta Geologica Sinica(English Edition) 2006年第4期80卷 550-556页

作者： cai chunfang PENG Licai MEI Bowen XIAO Yingkai Key Laboratory of Mineral Resources Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 Department of Engineering Mechanics Tsinghua University Beijing 100084 Yangtze University Jingzhou Hubei 434023 Qinghai Institute of Salt Lake Chinese Academy of Sciences Xining Qinghai 810008

In order to elucidate the origin and migration of basinal brines in the Bachu Bulge, Tarim Basin, we have carried out analyses on chemical composition, and boron, hydrogen and oxygen isotopes of formation waters together with the XRD of clay minerals from the Paleozoic strata. The waters show Ca, B, Li and Sr enrichment and SO4 depletion in the Carboniferous and Ordovician and K enrichment in part of the Ordovician relative to seawater. The relationship between δD and δ^18O shows that all the data of the waters decline towards the Global Meteoric Water Line with the intersection of them close to the present-day local meteoric water, suggesting that modern meteoric water has mixed with evaporated seawater. The ^87Sr/^86Sr ratios range from 0.7090 to 0.7011, significantly higher than those of the contemporary seawater. The δ^11B values range from ＋19.7 to ＋32.3‰, showing a decrease with the depth and B concentrations. The results suggest that isotopically distinct B and Sr were derived from external sources. However, since the percentages of illite are shown to increase with depth among clay minerals in the study area, i.e., illite is due to precipitation rather than leaching during deeper burial, it is unlikely for illite to have contributed a significant amount of B to the waters. Thus, B with low δ^11B values is interpreted to have been added mainly from thermal degradation of kerogen or the basalts in the Cambrian and Lower Ordovician.

关键词： δ^11B formation water water-rock interaction Tarim Basin

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Lipid Evidence for Oil Depletion by Sulfate-Reducing Bacteria during U Mineralization in the Dongsheng Deposit

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Journal of Earth Science 2018年第3期29卷 556-563页

作者： Tiankai Wang Lei Jiang chunfang cai Yuyang Yuan Yongdong Zhang Lianqi Jia Shuangling Chen Key Laboratory of Petroleum Resources Research Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Organic Geochemistry Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China School of Earth Sciences and Resources China University of GeosciencesBeijing 100083China

Fatty acids were extracted from fluid inclusions, and analyzed for distribution and individual 613C values to determine if there exists sulfate-reducing bacteria （SRB） and what substrates SRB depleted during U mineralization in the Dongsheng deposit. The 10-methylhexadecanoic acid （10Mel6：0） has been detected from most of the samples using GC-MS based on relative retention time and co-injection of standard 10Me16：0 compound. Samples with higher 10Me16： 0 concentrations, show higher U contents. The presence of 10Mel6：0 along with iso-ω7-cis-heptadecenoic acid （i17：1ω7c） and ω7-cisoctadecenoic acid （18：1ω7c） in the fluid inclusions may indicate the occurrence of SRB during the U mineralization. Saturated fatty-acids and unsaturated fatty-acids detected in this study have individual δ^13C values from-30.3‰ to-28.5‰ and-30.5‰ to-27.9‰, respectively. These values are close to those of n-alkanes of the associated oils and the bulk oils, but significantly distinct from methane, thus the oils are concluded to have been used as substrate for the microorganisms to survive on. This proposal is supported by oil biodegradation and ore-stage calcite cement with δ^13C values from-1.4‰ to-17.2‰.

关键词： fatty acids sulfate-reducing bacteria(SRB) individual lipid carbon isotope sulfur isotope uranium deposit.

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Redox condition during Ediacaran–Cambrian transition in the Lower Yangtze deep water basin, South China: constraints from iron speciation and δ^(13) Corg in the Diben section, Zhejiang

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Chinese Science Bulletin 2014年第28期59卷 3638-3649页

作者： Yuyang Yuan chunfang cai Tiankai Wang Lei Xiang Lianqi Jia Yan Chen Key Laboratory of Petroleum Resource Research Institute of Geology and Geophysics Chinese Academy of Sciences College of Earth Science University of Chinese Academy of Sciences Nanjing Institute of Geological Paleontology Chinese Academy of Sciences

Cherts of the Piyuancun Formation and black mudstones of the Hetang Formation in Diben Town, Kaihua County, Zhejiang were deposited in a deep water basin of the Lower Yangtze area during the Ediacaran–Cambrian transition(E–C transition), offering an opportunity to reconstruct the ancient redox condition of the deep water basin. Total 53 samples were collected and analyzed for iron speciation, including pyrite iron(FePy), carbonate associated iron(FeCarb), iron oxide and hydroxide(FeOx),magnetite iron(FeMag) and total iron(FeT) as well as d13 Corgto gain a deeper understanding of specific redox condition during the period. The results show that(1) most samples have pyrite concentration \1 %, with the FePy/FeHR ratio ranging from \0.01 to 0.81(mostly \0.5), and the FeHR/FeT ratio between 0.39 and 0.93(mostly [0.5).(2) The E–C boundary in the Diben section occurred at the maximum negative excursion of organic carbon isotope within the Piyuancun Formation, which can be correlated with other sections by using d13 Corg. We thus concluded that the relatively high content of highly reactive iron and low content of pyrite iron in the Diben section indicate a minor occurrence of H2 S and a predominance of anoxic and ferruginous environment, which is similar to other reported sections such as the Longbizui and Yanwutan sections, western Hunan. In addition, oxygen concentration in sea water showed an increasing trend in the interval corresponding to the bottom and upper layer of the Hetang Formation as indicated by the FeHR/FeT ratio, which decreased from 0.7 to 0.38, however, it was not great enough to oxidize the whole deep water. Therefore, during the E–C transition deep water basin in South China was most likely an anoxic and ferruginous enrironment, which prevented metazoon from the evolution in the deep water basin. Our work offers an important reference to better understanding of the spatiotemporal occurrence and dynamic processes of the ‘‘Cambrian Explosion''.

关键词：氧化还原状态寒武纪大爆发长江下游地区深水盆地中国南方铁形态迪卡浙江

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The Ocean Redox State Evolution and Its Controls during the Cambrian Series 1–2: Evidence from Lijiatuo Section, South China

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Journal of Earth Science 2016年第2期27卷 255-270页

作者： Lei Xiang chunfang cai Xunyun He Lei Jiang Yuyang Yuan Tiankai Wang Lianqi Jia Lei Yu Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences Institute of Geology and Geophysics Chinese Academy of Sciences Hangzhou Research Institute of Geology Petro China Exploration and Development Research Institute of Petro China Changqing Oilfield Company Petro China

Well-exposed Lijiatuo Section was chosen to explore the temporal evolution and controls of the oceanic redox state, primary productivity and seawater sulfate levels during the Cambrian Series 1-2, South China. This section consists of Xiaoyanxi Formation（Fm.） mudstones and Liuchapo Fm. cherts that deposited in the slope and basin environment. Five oxic-anoxic cycles were identified based on V/Sc, Th/U and the enrichment factors of Mo, U, V, Ni and Cu. The Middle-Upper Liuchapo Fm. and the Middle Xiaoyanxi Fm. were deposited under oxic-suboxic conditions, and the rest of the strata were under anoxic conditions. The Re/Mo ratio demonstrated that the oxic-suboxic conditions in the Middle Xiaoyanxi Fm. were accompanied by transient sulfidic conditions, and the rest of the section was underanoxic and non-sulfidic conditions. All the TOC and the enrichment factors of Ba, Ni, Cu, Zn and Cd demonstrated that both the sinking and burial flux of organic matter（OM） in Liuchapo Fm. were lower than that in the overlying Xiaoyanxi Fm. The highest sinking and burial flux of OM in the Xiaoyanxi Fm. appeared at its lower parts; however, the lowest sinking and burial flux of OM in the Xiaoyanxi Fm. appeared in its middle parts. TOC/TS, TS and the vertical trend of δ^（34）S_（py） demonstrated that the seawater was dominated by low oceanic sulfate levels, which resulted in the absence of free H_2S. The rise of the atmospheric oxygen content may be the principal driver for the associated, transient suboxic-oxic and nearly sulfidic environment in the middle Xiaoyanxi Fm.

关键词： trace element redox state seawater sulfate level Cambrian Series 1-2 South China.

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用矿物元素指纹识别中华绒螯蟹产地及其养殖水体

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水产学杂志 2024年第1期37卷 9-15页

作者：宋学宏黄芳梁凤玲胡建国陆彬蔡春芳苏州大学基础医学与生物科学学院江苏苏州215123 苏州市农产品质量安全监测中心江苏苏州215104 苏州市阳澄湖大闸蟹行业协会江苏苏州215137

分析测定体质量为180~200 g左右、产自阳澄湖湖区(YCL)、阳澄湖周边池塘(YCP)、洪泽湖(HZL)、高邮湖(GYL)、固城湖(GCL)湖区的雄性中华绒螯蟹(Eriocheir sinensis)体内Ca、Mg、K、Na、Sr和Fe等35种元素的含量,以开发中华绒螯蟹产地及其... 详细信息

分析测定体质量为180~200 g左右、产自阳澄湖湖区(YCL)、阳澄湖周边池塘(YCP)、洪泽湖(HZL)、高邮湖(GYL)、固城湖(GCL)湖区的雄性中华绒螯蟹(Eriocheir sinensis)体内Ca、Mg、K、Na、Sr和Fe等35种元素的含量,以开发中华绒螯蟹产地及其养殖水体的识别技术。结果表明,32种元素组间差异显著(P<0.05)。基于35种元素对中华绒螯蟹来源进行判别分析,10种元素进入判别模型,测试集和交叉验证判别准确率分别为98.3%和98.3%,仅有11.1%的HZL蟹被判入地理距离较近的GYL。剔除Ca、Mg、K、Na、Fe、Mn、Zn、Cu这8种在中华绒螯蟹养殖过程中人为输入量较大的元素再行判别分析,则6种元素进入判别模型,测试集和交叉验证判别准确率分别为100.0%和96.7%,仍有11.1%的HZL蟹被判入GYL,还有11.1%的GCL蟹被判入GYL。上述结果表明,采用矿物元素指纹识别中华绒螯蟹产地和养殖水体具有较高的准确率,养殖过程中人为输入量较大的元素对中华绒螯蟹产地与养殖水体的识别无不利影响。

关键词：中华绒螯蟹元素指纹产地养殖水体判别分析

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Biological manipulation of eutrophication in West Yangchen Lake

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Aquaculture and Fisheries 2019年第5期4卷 190-197页

作者： chunfang cai Jie He Wen Chen Junbiao Zhang Qianqian Wang Xuehong Song Yuantu Ye Yongling Wang Ping Wu Xiamin Cao School of Biology and Basic Medical Sciences Soochow UniversitySuzhou215123China

This paper aims to share findings on nonclassical biomanipulation from a field study on eutrophic West Yangcheng(WY)*** the forty years before 2009,cyanobacteria frequently bloomed in WY Lake,an event which has not occurred since *** stocking density of filter-feeding carp was estimated to be more than 50 g/m^(3) in July after 2009,approximately double the amount observed before 2009,and the ratio of silver carp to bighead carp(SBR)increased from 0.8 to more than *** with the water quality between 2004 and 2007,the total nitrogen(TN)and the chlorophyll-a(Chl-a)concentration decreased while total phosphorus(TP)and transparency(SD)increased significantly after 2009(P0.05).A significant negative correlation was observed between fish yield and SD(P<0.05).The results also showed that filter-feeding carp more effectively restrained algae growth when SD was *** results indicated that a high density of filter-feeding fish with increased SBR interfered with the driving effects of nutrients and temperature on the growth of algal biomass and then restrained bloom of *** may be necessary to take SD into consideration for determining the appropriate fish stocking density for the biomanipulation of algae.

关键词： Biomanipulation West yangchen Lake Algal bloom Filter-feeding fish Stocking density Ratio of silver carp to bighead carp

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建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

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