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Molecular Plant 2015年第3期8卷 399-411页

作者： chun-lin yang Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Plant Genomics Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

In this study, we identified a defense-related major latex protein （MLP） from upland cotton （designated GhMLP28） and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jas- monic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing re- sulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 （GhERF6） and facilitated the binding of GhERF6 to GCC-box element. Transient expression assay demonstrated that GhMLP28 enhanced the tran- scription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants.

关键词： major latex protein ethylene responsive factor interaction disease tolerance cotton

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教育叙事创新写作的实践路径

教学与管理 2024年第8期 22-25页

作者：林春阳江苏省连云港市灌南县张店镇中心小学

教育叙事是教师记录生活、反思教育、表达感悟的写作文体,是教师完善教育理念、提升写作水平、促进学术交流的重要途径。当前,许多教师虽教学经验丰富,却写不出有新意的教育叙事,而在选题、选材、策略、改法等方面寻求创新突破,能有效... 详细信息

教育叙事是教师记录生活、反思教育、表达感悟的写作文体,是教师完善教育理念、提升写作水平、促进学术交流的重要途径。当前,许多教师虽教学经验丰富,却写不出有新意的教育叙事,而在选题、选材、策略、改法等方面寻求创新突破,能有效提升教育叙事的写作质量。教育叙事的创新写作,可以进一步促使教师实现专业成长,建立职业自信,对于推动教育事业的发展具有深远意义。

关键词：教育叙事创新写作选题素材教师专业成长

Luminescence property and magnetic resonance imaging of Gd_(2)O_(3):Tb^(3+) nanocrystals doped with Zn^(2+),Li^(+)

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Rare Metals 2021年第8期40卷 2049-2058页

作者： Rong Fu Yan-Yan Hu Hui-Na Qiao chun-lin yang Hao Yin Mei-Gui Ou College of Materials and Metallurgy University of GuizhouGuiyang550025China National Local Co-construction Engineering Laboratory for High Performance Metal Structure Material and Manufacture Technology Guiyang550025China College of Chemistry and Materials Engineering Guiyang UniversityGuiyang550003China Department of Neurosurgery Guizhou Provincial People’s HospitalGuiyang550002China

Gd_(2)O_(3):Tb^(3+) nanocrystal co-doped with Zn^(2+)and Li^(+) was prepared by the combination of polyol and solid-phase methods.The synthesized nanoparticles were characterized by X-ray diffraction(XRD),field emission scanning electron microscopy(FESEM),transmission electron microscopy(TEM),photoluminescence(PL)spectra and magnetic resonance imaging(MRI).The results revealed that both Gd_(2)O_(3):Tb^(3+)/Zn^(2+) and Gd_(2)O_(3):Tb^(3+)/Li^(+) had cubic unit structures with a spherical shape,and the fluorescence intensity of the nanoparticles reached a maximum at a doping concentration of0.050 mmol%for Zn^(2+) and 2 mol%for Li^(+),respectively.In addition,the T_(1)/T_(2)(longitudinal/transverse relaxation time)-weighted MRI signals got enhanced due to the increased longitudinal relaxivity(r1)and transverse relaxivity(r2)after the doping by metal ions.Therefore,the materials reported here can be used for fluorescence and MR bimodal imaging,as tracers and for fluorescence detections.

关键词： Gd_(2)O_(3):Tb^(3+) Zn^(2+)/Li^(+)doping Luminescent property Magnetic resonance imaging Nanomaterials

The role of innate immunity in diabetic nephropathy and their therapeutic consequences

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Journal of Pharmaceutical Analysis 2024年第1期14卷 39-51页

作者： Min yang chun Zhang Department of Nephrology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China

Diabetic nephropathy (DN) is an enduring condition that leads to inflammation and affects a substantial number of individuals with diabetes worldwide. A gradual reduction in glomerular filtration and emergence of proteins in the urine are typical aspects of DN, ultimately resulting in renal failure. Mounting evidence suggests that immunological and inflammatory factors are crucial for the development of DN. Therefore, the activation of innate immunity by resident renal and immune cells is critical for initiating and perpetuating inflammation. Toll-like receptors (TLRs) are an important group of receptors that identify patterns and activate immune responses and inflammation. Meanwhile, inflammatory responses in the liver, pancreatic islets, and kidneys involve inflammasomes and chemokines that generate pro-inflammatory cytokines. Moreover, the activation of the complement cascade can be triggered by glycated proteins. This review highlights recent findings elucidating how the innate immune system contributes to tissue fibrosis and organ dysfunction, ultimately leading to renal failure. This review also discusses innovative approaches that can be utilized to modulate the innate immune responses in DN for therapeutic purposes.

关键词： Innate immunity Diabetic nephropathy Inflammation Toll-like receptor Inflammasomes

Mechanically driven assembly of biomimetic 2D-material microtextures with bioinspired multifunctionality

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Nano Research 2024年第2期17卷 663-678页

作者： yang Li lin Jing Joshua M.Little Haochen yang Tsai-chun chung Po-Yen Chen Department of Chemical and Biomolecular Engineering University of MarylandCollege ParkMD 20740USA Maryland Robotics Center University of MarylandCollege ParkMD 20742USA School of Materials Science and Engineering Nanyang Technological University50 Nanyang AvenueSingapore 639798Singapore

Nature provides a wealth of bio-inspiration for advanced material research.Assembling various nanomaterials into biomimetic microtextures with bioinspired functionalities has spurred increasing research interests and facilitated technological advances in various applications.In recent years,two-dimensional materials(2DMs)have emerged as important building block units in the biomimicry field due to their distinct chemical,physical,electrical,electrochemical,and catalytic properties.In this review article,various mechanically driven assembly approaches are summarized to fabricate various genealogies of biomimetic 2DM microtextures with bio-inspired multifunctionality.First,sequential deformation strategies are discussed to programmably construct higher dimensional 2DM microtextures,ranging from wrinkles/crumples(one-time deformation)to multiscale hierarchies(multiple deformations).Next,the current progress using higher dimensional 2DM microtextures to imitate different biological structures and/or induce bio-inspired multifunctionality is systematically summarized.Four showcases of bio-inspiration and biomimicry using different 2DM nanosheets are highlighted:(1)wrinkle patterns of an earthworm that spur the design of strain sensors with programmable working ranges and sensitivities,(2)wrinkle appearance of a Shar-Pei dog that motivates the fabrication of stretchable energy storage devices,(3)hierarchical scale textures of a desert lizard that inspire cation-induced gelation platforms for 2DM aerogels,and(4)wrinkle skin of an elephant that influences the development of 2DM protective skin for soft robots.Finally,challenges and future opportunities of adopting 2DM nanosheets to assemble biomimetic microstructures with synergistic functionalities are discussed.

关键词： two-dimensional(2D)materials mechanically driven assembly biomimetic microtextures functional nanocomposite bio-inspired multifunctionality

Assembly of highly efficient aqueous light-harvesting system from sequence-defined peptoids for cytosolic microRNA detection

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Nano Research 2024年第2期17卷 788-796页

作者： yang Song Xiaoli Cai Mingming Wang Dan Du Yuehe lin chun-Long Chen Department of Mechanical and Materials Engineering Washington State UniversityPullmanWA 99164USA Division of Physical Sciences Pacific Northwest National LaboratoryRichlandWA 99352USA Department of Chemical Engineering University of WashingtonSeattleWA 98195USA

Precisely controlled spatial distributions of artificial light-harvesting systems in aqueous media are of significant importance for mimicking natural light-harvesting systems;however,they are often restrained by the solubility and the aggregation-caused quenching effect of the hydrophobic chromophores.Herein,we report one highly efficient artificial light-harvesting system based on peptoid nanotubes that mimic the hierarchical cylindrical structure of natural systems.The high crystallinity of these nanotubes enabled the organization of arrays of donor chromophores with precisely controlled spatial distributions,favoring an efficient Förster resonance energy transfer(FRET)process in aqueous media.This FRET system exhibits an extremely high efficiency of 98.6%with a fluorescence quantum yield of 40%and an antenna effect of 29.9.We further demonstrated the use of this artificial light-harvesting system for quantifying miR-210 within cancer cells.The fluorescence intensity ratio of donor to acceptor is linearly related to the concentration of intercellular miR-210 in the range of 3.3–156 copies/cell.Such high sensitivity in intracellular detection of miR-210 using this artificial light-harvesting system offers a great opportunity and pathways for biological imaging and detection,and for the further creation of microRNA(miRNA)toolbox for quantitative epigenetics and personalized medicine.

关键词： light-harvesting peptoid nanotube Förster resonance energy transfer intracellular RNA detection

Construction of algal-bacterial consortia using green microalgae Chlorella vulgaris and As(Ⅲ)-oxidizing bacteria:As tolerance and metabolomic profiling

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Journal of Environmental Sciences 2024年第5期139卷 258-266页

作者： Xiaoman He Guobing lin Jiayuan Zeng Zhaoguang yang lin Wang College of Chemistry and Chemical Engineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety Central South UniversityChangsha 410083China

Bioremediation became a promising technology to resolve arsenic(As)contamination in aquatic environment.Since monoculture such as microalgae or bacteria was sensitive to environmental disturbance and vulnerable to contamination,green microalgae Chlorella vulgaris and arsenite(As(Ⅲ))-oxidizing bacteria Pseudomonas sp.SMS11 were co-cultured to construct algal-bacterial consortia in the current study.The effects of algae-bacteria(A:B)ratio and exposure As(Ⅲ)concentration on algal growth,As speciation and metabolomic profile were investigated.Algal growth arrested when treated with 100 mg/L As(Ⅲ)without the co-cultured bacteria.By contrast,co-cultured with strain SMS11 significantly enhanced As tolerance in C.vulgaris especially with A:B ratio of 1:10.All the As(Ⅲ)in culture media of the consortia were oxidized into As(Ⅴ)on day 7.Methylation of As was observed on day 14.Over 1% and 0.5% of total As were converted into dimethylarsinic acid(DMA)after 21days cultivation when the initial concentrations of As(Ⅲ)were 1 and 10 mg/L,respectively.Metabolomic analysis was further performed to reveal the response of consortia metabolites to external As(Ⅲ).The enriched metabolomic pathways were associated with carbohydrate,amino acid and energy metabolisms.Tricarboxylic acid cycle and glyoxylate and dicarboxylate metabolism were upregulated under As stress due to their biological functions on alleviating oxidative stress and protecting cells.Both carbohydrate and amino acid metabolisms provided precursors and potential substrates for energy production and cell protection under abiotic stress.Alterations of the pathways relevant to carbohydrate or amino acid metabolism were triggered by energy requirement.

关键词： Microalgae Algal-bacterial consortia As(Ⅲ)-oxidizing bacteria Metabolomics Energy metabolism

Luminescence and magnetic properties of bifunctional nanoparticles composited by nitrogen-doped graphene quantum dots and gadolinium

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Journal of Rare Earths 2024年第4期42卷 716-723页

作者： Wanli Zhang Hao Zhou Meigui Ou Donghao Sun chunlin yang College of Materials and Metallurgy University of GuizhouGuiyang550025China National Local Co-construction Engineering Laboratory for High Performance Metal Structure Material and Manufacture Technology Guiyang550025 China College of Chemistry and Materials Engineering Guiyang UniversityGuiyang550003China

In this paper,nitrogen-doped graphene quantum dots(N-GQDs)were combined with gadolinium ions(Gd^(3+))by a surface modification to obtain magneto-optical dual-functional N-GQDs/Gd^(3+)nanoparticles.The morphology of obtained composite was characterized by field emission scanning electron microscopy and transmission electron microscopy.Luminescence and magnetic properties were measured by a fluorescence spectrophotometer and a vibrating sample magnetometer,respectively.Results indicate that well-dispersed spherical N-GQDs/Gd^(3+)nanoparticles have an average diameter of 7 nm.N-doping significantly increases the luminesce nce of particles with an optimal luminescence intensity at 20℃and pH=9.X-ray photoelectron spectroscopy results indicate that the N-doping introduces pyrrolic N as an electron donor,enhancing fluorescence by increasing the surface electron cloud density of N-GQDs.In addition,density functional theory calculation results reveal that N-doping reduces the band gap of NGQDs/Gd^(3+),enabling electronic transitions to higher energy levels and generating more activation sites,thereby enhancing luminescence.Compared to N-GQDs/Gd^(3+)prepared at 20℃,the saturated magnetization of particles prepared at 40℃is 0.85 emu/g,indicating a better magnetic response.The above results suggest that bifunctional nanomaterials N-GQDs/Gd^(3+)with excellent optical properties and magnetism can be better used for fluorescence and magnetic resonance imaging.

关键词： Nitrogen-doped graphenequantum dots Gadolinium Luminescence Magnetism Rare earths

Thickness dependence of superconductivity in layered GeP_(5)

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Rare Metals 2024年第3期43卷 1323-1328页

作者： lin Wu Ke-Meng yang Wei Gan Rui-chun Xiao Ya-Jie Dai Xi Tang Hong lin Hui Han Hui Li Information Materials and Intelligent Sensing Laboratory of Anhui Province Institute of Physical Science and Information TechnologyAnhui UniversityHefei230601China

Understanding the relationship between dimensionality and superconductivity has attracted substantial research interest.In this work,we report the effective modulation of superconductivity in GeP_(5)through dimensionality.The superconducting phase transition temperature improves by~10%as the thickness of GeP_(5)decreases from bulk to~96 nm,behaving as a unique negative relationship between the superconducting phase transition temperatureand thickness.

关键词： superconductivity dimensionality

Magnitude and direction of temperature variability affect hospitalization for myocardial infarction and stroke:population-based evidence from Guangzhou,China

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Frontiers of Environmental Science & Engineering 2024年第3期18卷 1-11页

作者： Zhou yang Murui Zheng Ze-lin Yan Hui Liu Xiangyi Liu Jie-Qi Jin Jiagang Wu chun-Quan Ou State Key Laboratory of Organ Failure Research Department of BiostatisticsGuangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou 510515China Guangzhou Center for Disease Control and Prevention Guangzhou 510440China

Relationships between nonoptimal temperatures and cardiovascular disease(CVD)mortality have been well documented.However,evidence of the association between temperature variability(TV)and CVD morbidity is limited.This study aimed to quantify the risk and burden of CVD-related hospitalization associated with the magnitude and direction of TV.Data on meteorology and population-based hospitalizations for myocardial infarction(MI)and stroke were collected in Guangzhou,China,from 2013 to 2017.Hourly temperature variability(HTV)was measured as the standard deviation of hourly temperature records over specific exposure days.The direction(upward or downward)of HTV was defined as the average daily mean temperature change relative to that of the previous day during the exposure period.Quasi-Poisson regression was applied to assess the impact of HTV after adjusting for the daily mean temperature,and the hospitalization fractions attributable to HTV were calculated.A 1℃-increase in HTV was significantly associated with a 2.24%and 1.72%increase in hospitalizations for MI and hemorrhagic stroke(HS)at lag 0–1 d,respectively,and a 1.55%increase in hospitalizations for ischemic stroke(IS)at lag 0–3 d.During the study period,5.99%,4.64%,and 4.53%of MI,HS,and IS hospitalizations,respectively,were attributable to HTV.The upward TV exerts acute effects on CVD hospital admissions,whereas the impact of downward TV generally lags.These findings highlight the importance of the magnitude and direction of temperature fluctuations,in addition to the mean level,in assessing the adverse health impacts of temperature variations.

关键词： Hourly temperature variability Cardiovascular Hospitalization Direction China