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Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells

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World Journal of Gastroenterology 2005年第30期11卷 4628-4633页

作者： chun-hong zhao qi-fu li Laboratory of Cell Biology the Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 Fujian Province China

AIM： To find and identify specific nuclear matrix proteins associated with proliferation and differentiation of carcinoma cells, which will be potential markers for cancer diagnosis and targets in cancer therapy. METHODS： Nuclear matrix proteins were selectively extracted from MGcS0-3 cells treated with or without hexamethylamine bisacetamide （HMBA）, and subjected to 2-D gel electrophoresis. The resulted protein patterns were analyzed by Melanie software. Spots of nuclear matrix proteins differentially expressed were excised and subjected to in situ digestion with trypsin. Peptide masses were obtained by matrix-assisted laser-desorption/ ionization time of flight mass spectrometry （MALDI-TOFMS） analysis and submitted for database searching using Mascot tool. RESULTS： The MGc80-3 cells were induced into differentiation by HMBA. There were 22 protein spots which changed remarkably in the nuclear matrix, from differentiation of MGcS0-3 cells compared to control. Eleven of which were identified. Seven proteinsactin, prohibitin, porin 31HL, heterogeneous nuclear dbonucleoprotein A2/B1, vimentin, ATP synthase, and heat shock protein 60 were downregulated, whereas three proteins - heat shock protein gp96, heat shock protein 90-beta, and valosin-containing protein were upregulated, and the oxygen-regulated protein was only found in the differentiated MGc80-3 cells. CONCLUSION： The induced differentiation of carcinoma cells is accompanied by the changes of nuclear matrix proteins. further characterization of those proteins will show the mechanism of cellular proliferation and differentiation, as well as cancer differentiation.

关键词： Nuclear matrix proteins Cell differentiation Human gastric mucous adenocarcinoma MGc80-3 Hexamethylamine bisacetamide

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Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin li Ting Zhang Yanlin fu Peiyan Long Xiao Gao Zhengwei Wang Zhizhong Guan Xiaolan qi Wei hong Yan Xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

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The crosstalk between cellular survival pressures and N6-methyladenosine modification in hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 67-75页

作者： Chu-li fu Zheng-Wei zhao qiang-Nu Zhang Department of Hematology and Oncology Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Biomedicine Research Center Guangdong Provincial Key Laboratory of Major Obstetric DiseaseGuangdong Provincial Clinical Research Center for Obstetrics and Gynecologythe Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150China

Background:Within the tumor microenvironment,survival pressures are prevalent with potent drivers of tumor progression,angiogenesis,and therapeutic resistance.N6-methyladenosine(m6 A)methylation has been recognized as a critical post-transcriptional mechanism regulating various aspects of mRNA *** the intricate interplay between survival pressures and m6 A modification pro-vides new insights into the molecular mechanisms underlying hepatocellular carcinoma(HCC)progres-sion and highlights the potential for targeting the survival pressures-m6 A axis in HCC diagnosis and *** sources:A literature search was conducted in PubMed,MEDliNE,and Web of Science for relevant articles published up to April *** keywords used for the search included hepatocellular carcinoma,cellular survival,survival pressure,N6-methyladenosine,tumor microenvironment,stress response,and ***:This review delves into the multifaceted roles of survival pressures and m6 A RNA methylation in HCC,highlighting how survival pressures modulate the m6 A landscape,the impact of m6 A modifica-tion on survival pressure-responsive gene expression,and the consequent effects on HCC cell survival,proliferation,metastasis,and resistance to ***,we explored the therapeutic potential of targeting this crosstalk,proposing strategies that leverage the understanding of survival pressures and m6 A RNA methylation mechanisms to develop novel,and more effective treatments for ***:The interplay between survival pressures and m6 A RNA methylation emerges as a complex regulatory network that influences HCC pathogenesis and progression.

关键词： Hepatocellular carcinoma N6-methyladenosine methylation Hypoxia Target therapy Survival pressure

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Introducing strong metal–oxygen bonds to suppress the Jahn-Teller effect and enhance the structural stability of Ni/Co-free Mn-based layered oxide cathodes for potassium-ion batteries

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Journal of Energy Chemistry 2025年第2期101卷 713-722页

作者： Yicheng lin Shaohua Luo Pengyu li Jun Cong Wei zhao lixiong qian qi Sun Shengxue Yan School of Materials Science and Engineering Northeastern University School of Resources and Materials Northeastern University at Qinhuangdao Key Laboratory of Dielectric and Electrolyte functional Material Hebei Province

Mn-based layered oxides（KMO） have emerged as one of the promising low-cost cathodes for potassiumion batteries（PIBs）.However,due to the multiple-phase transitions and the distortion in the MnO6structure induced by the Jahn-Teller（JT） effect associated with Mn-ion,the cathode exhibits poor structural ***,we propose a strategy to enhance structural stability by introducing robust metal-oxygen（M-O） bonds,which can realize the pinning effect to constrain the distortion in the transition metal（TM） ***,all the elements employed have exceptionally high crustal *** a proof of concept,the designed K0.5Mn0.9Mg0.025Ti0.025Al0.05O2cathode exhibited a discharge capacity of approximately 100 mA h g-1at 20 mA g-1with 79% capacity retention over 50 cycles,and 73% capacity retention over 200 cycles at 200 mA g-1,showcased much better battery performance than the designed cathode with less robust M-O *** properties of the formed M-O bonds were investigated using theoretical *** enhanced dynamics,mitigated JT effect,and improved structural stability were elucidated through the in-situ X-ray diffractometer（XRD）,in-situ electrochemical impedance spectroscopy（EIS）(and distribution of relaxation times（DRT） method),and ex-situ X-ray absorption fine structure（XAFS） *** study holds substantial reference value for the future design of costeffective Mn-based layered cathodes for PIBs.

关键词： Jahn-Teller effect Layered oxide cathodes Low-cost Potassium-ion batteries Robust M–O bonds

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Spatial Patterns and Controlling Factors of Soil Organic Carbon and Total Nitrogen in the Three River Headwaters Region,China

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Chinese Geographical Science 2025年第1期35卷 131-148页

作者： CUI qiao li Zongxing FENG qi ZHANG Baijuan zhao Yue Observation and Research Station of Eco-Hydrology and National Park by Stable Isotope Tracing in qilian Mountains/Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China College of Geography and Environmental Science Northwest Normal UniversityLanzhou 730070China

The alpine ecosystem has great potential for carbon *** organic carbon(SOC)and total nitrogen(TN)are highly sensitive to climate change,and their dynamics are crucial to revealing the effect of climate change on the structure,function,and services of the ***,the spatial distribution and controlling factors of SOC and TN across various soil layers and vegetation types within this unique ecosystem remain inadequately *** this study,256 soil samples in 89 sites were collected from the Three River Headwaters Region(TRHR)in China to investigate SOC and TN and to explore the primary factors affecting their distribution,including soil,vegetation,climate,and geography *** results show that SOC and TN contents in 0-20,20-40,40-60,and 60-80 cm soil layers are 24.40,18.03,14.04,12.40 g/kg and 2.46,1.90,1.51,1.17 g/kg,respectively;with higher concentrations observed in the southeastern region compared to the northwest of the ***-way analysis of variance reveals that SOC and TN levels are elevated in the alpine meadow and the alpine shrub relative to the alpine steppe in the 0-60 cm soil *** structural equation model explores that soil water content is the main controlling factor affecting the variation of SOC and ***,the geography,climate,and vegetation factors notably indirectly affect SOC and TN through soil ***,it can effectively improve soil water and nutrient conditions through vegetation restoration,soil improvement,and grazing management,and the change of SOC and TN can be fully understood by establishing monitoring networks to better protect soil carbon and nitrogen.

关键词： controlling factors different soil layers soil organic carbon(SOC) soil total nitrogen(TN) alpine ecosystem the Three River Headwaters Region(TRHR),China

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Changes in source contributions to the oxidative potential of PM_(2.5)in urban Xiamen,China

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Journal of Environmental Sciences 2025年第3期149卷 342-357页

作者： Jia-Min li Si-Min zhao qi-Yu Miao Shui-Ping Wu Jie Zhang James J.Schwab fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies Xiamen UniversityXiamen 361102China Center for Marine Environmental Chemistry and Toxicology College of Environment and EcologyXiamen UniversityXiamen 361102China Atmospheric Sciences Research Center University at AlbanySUNYAlbany 12226USA

The toxicity of PM_(2.5)does not necessarily change synchronously with its mass *** this study,the chemical composition(carbonaceous species,water-soluble ions,and metals)and oxidative potential(dithiothreitol assay,DTT)of PM_(2.5)were investigated in 2017/2018 and 2022 in Xiamen,*** decrease rate of volume-normalized DTT(DTTv)(38%)was lower than that of PM_(2.5)(55%)between the two sampling ***,the mass-normalized DTT(DTTm)increased by 44%.Clear seasonal patterns with higher levels in winter were found for PM_(2.5),most chemical constituents and DTTv but not for *** large decrease in DTT activity(84%−92%)after the addition of EDTA suggested that watersoluble metals were the main contributors to DTT in *** increased gap between the reconstructed and measured DTTv and the stronger correlations between the reconstructed/measured DTT ratio and carbonaceous species in 2022were *** decrease rates of the hazard index(32.5%)and lifetime cancer risk(9.1%)differed from those of PM_(2.5)and DTTv due to their different main *** PMF-MLR model showed that the contributions(nmol/(min·m^(3)))of vehicle emission,coal+biomass burning,ship emission and secondary aerosol to DTTv in 2022 decreased by 63.0%,65.2%,66.5%,and 22.2%,respectively,compared to those in 2017/2018,which was consistent with the emission reduction of vehicle exhaust and coal consumption,the adoption of low-sulfur fuel oil used on board ships and the reduced production of ***,the contributions of dust+sea salt and industrial emission increased.

关键词： Chemical composition Oxidative potential Interannual change PMF-MLR Source apportionment

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Revealing the Role of Hydrogen in Highly Efficient Ag-Substituted CZTSSe Photovoltaic Devices:Photoelectric Properties Modulation and Defect Passivation

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Nano-Micro Letters 2025年第4期17卷 166-180页

作者： Xiaoyue zhao Jingru li Chenyang Hu Yafang qi Zhengji Zhou Dongxing Kou Wenhui Zhou Shengjie Yuan Sixin Wu The Key Laboratory for Special functional Materials of MOE School of Nanoscience and Materials EngineeringNational and Local Joint Engineering Research Center for High-Efficiency Display and Lighting TechnologyCollaborative Innovation Center of Nano Functional Materials and ApplicationsHenan UniversityKaifeng 475004People’s Republic of China

The presence of SnZn-related defects in Cu_(2)ZnSn(S,Se)_(4)(CZTSSe)absorber results in large irreversible energy loss and extra irreversible electron-hole non-radiative recombination,thus hindering the efficiency enhancement of CZTSSe *** the incorporation of Ag in CZTSSe can effectively suppress the SnZn-related defects and significantly improve the resulting cell performance,an excellent efficiency has not been achieved to date primarily owing to the poor electrical-conductivity and the low carrier density of the CZTSSe film induced by Ag ***,this study exquisitely devises an Ag/H co-doping strategy in CZTSSe absorber via Ag substitution programs followed by hydrogen-plasma treatment procedure to suppress SnZn defects for achieving efficient CZTSSe ***-depth investigation results demonstrate that the incorporation of H in Ag-based CZTSSe absorber is expected to improve the poor electrical-conductivity and the low carrier density caused by Ag ***,the C=O and O-H functional groups induced by hydrogen incorporation,serving as an electron donor,can interact with under-coordinated cations in CZTSSe material,effectively passivating the SnZn-related ***,the incorporation of an appropriate amount of Ag/H in CZTSSe mitigates carrier non-radiative recombination,prolongs minority carrier lifetime,and thus yields a champion efficiency of 14.74%,showing its promising application in kesterite-based CZTSSe devices.

关键词： CZTSSe Ag/H co-doping Photoelectric properties modulation Defect passivation Non-radiative recombination

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Dual-Mode Colorimetric and Fluorescent Detection of Tumor Cells Based on Gold Nanoparticles-Loaded Phosphine Covalent Organic Frameworks

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Journal of Analysis and Testing 2025年第01期 21-31页

作者： Bilian li Min Zhou chun zhao lin Xiao Tianle qi Hanbin Xu linjiang Guo Guobao Ning Xiaokang Lu Keming Zhu Hui zhao Can-Peng li School of Chemical Science and Technology Yunnan University Affiliated Hospital of Yunnan University School of life Science Yunnan University Laboratory for Conservation and Utilization of Bioresource Yunnan University

Circulating tumor cells(CTCs) are important markers for cancer. The part of tumor cells that are detached from the primary tumor or metastatic tumor and enter the blood circulation is called CTCs. It is crucial to develop a rapid, accurate, and easy-to-implement diagnostic system for CTCs for early tumor diagnosis and for monitoring progression of the disease. In this work, we reported a colorimetric and fluorescent dual-mode assay for the detection of CTCs. Our assay used magnetic nanoparticles and aptamer for CTCs capture and gold nanoparticles-loaded covalent organic frameworks(Au@COFs) for signal amplification, respectively. The magnetic nanoparticles were modified with folic acid to capture CTCs by interaction between folic acid and the folate receptor overexpressed on the surface of tumor cells. The covalent organic frameworks were engineered to have both nitro-reductase-like and glucose–oxidase-like activities. The nitro-reductase-like activity converted the substrate p-nitrophenol to p-nitroaniline for colorimetric detection, and the glucose-oxidase-like activity enabled fluorescence detection. Specifically, Au@COFs catalyzed glucose oxidation and generated hydrogen peroxide to oxidize Fe 2+to Fe 3+, which converted MIL(Al)-MOF to MIL(Fe)-MOF through ion exchange, resulting in the fluorescence quenching of MIL(Al)-MOF. Our assay showed high sensitivity with a detection limit of 17 cells/mL using MCF-7 cells as model cancer cells. This work provided an e fficient and ultrasensitive strategy for CTCs detection and has potential applications in cancer identification and diagnosis. Dual-mode detection system, combining colorimetric and fluorescent signals, integration of magnetic nanoparticles and aptamers for CTC capture, and utilization of covalent organic frameworks loaded with gold nanoparticles for signal amplification can improve sensitivity and accuracy.

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A nitrogen-rich halogen-bonded organic framework (XOF) for efficient iodine capture and security printing

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Science China Chemistry 2025年第2期68卷 631-640页

作者： qi zhao Penghao Sun Guanfei Gong Yi Chen lingzhi Luo Yongfei Yin chunsheng li Jike Wang Lu Wang Shigui Chen The Institute for Advanced Studies Wuhan UniversityWuhan 430072China Ningxia Vocational Technical College of Industry and Commerce Yinchuan 750100China Key Laboratory of Advanced Electrode Materials for Novel Solar Cells for Petroleum and Chemical Industry of China School of Chemistry and Life SciencesSuzhou University of Science and TechnologySuzhou 215009China

The escalating utilization of nuclear energy and nuclear medicine raises concerns about the environmental impact of radioactive iodine,necessitating the development of effective iodine adsorbents,especially under a real-world scenario with extremely low iodine concentration and elevated ***,we have presented the construction of a nitrogen-abundant two-dimensional(2D)halogen-bonded organic framework,XOF-TNP,characterized by exceptional crystallinity,thermal stability,and nitrogen-rich ***-TNP exhibits strong binding to iodine,thanks to the fact that iodine can be pre-enriched into the framework through N…I *** nitrogen-rich framework and I^(+) synergistically have extremely high binding force to iodine,enabling the rapid and efficient capture of iodine in both vapor and solution phases,with significant *** flow-through adsorption experiments using an XOF-TNP-packed column achieve 99%iodine removal from hexane and aqueous solutions,surpassing traditional activated *** highlights its potential for environmental *** enables the development of a novel rewritable security paper,utilizing its iodine adsorption properties to encrypt and decrypt QR *** research expands the application scope of halogen-bonded organic frameworks,providing insights into the design of materials for environmental remediation and security applications.

关键词： [N…I+…N]halogen bond halogen-bonded organic framework iodine capture security printing supramolecular assembly

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New chapter in reform and development of organ donation and transplantation in China: Embracing past, grounding in national conditions, upholding steadfast belief, and looking forward to future

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 6-13页

作者： hong-Tao zhao Shu-Sen Zheng Jia Fan Jia-hong Dong Zhong-Hua Chen Wu-Jun Xue qi-Fa Ye Hai-Bo Wang Jing-Yu Chen Zhe Zheng Feng Huo Xiao-Shun He Miao Pu Jie zhao Jie-fu Huang China Organ Transplantation Development Foundation Beijing 100010China China National Human Organ Donation and Transplantation Committee Beijing 100010China The First Affiliated Hospital Zhejiang University School of MedicineHangzhou 310003China Zhongshan Hospital Fudan UniversityShanghai 200032China Beijing Tsinghua Changgung Hospital Affiliated to Tsinghua University Beijing 102218China Tongji Hospital Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430014China The First Affiliated Hospital of Xi’an Jiaotong University Xi’an 710061China Zhongnan Hospital of Wuhan University Wuhan 430000China Wuxi People’s Hospital Wuxi 214023China fuwai Hospital Chinese Academy of Medical SciencesBeijing 100037China General Hospital of Southern Theater Command of PLA Guangzhou 510120China The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080China

The reform stems from honesty and determination. Since 2005, organ donation and transplantation in China has undergone thorough reform, which complies with legislation requirements and ethical principles established by the World Health Organization(WHO). Reform in China has demonstrated the unwavering confidence and utmost determination of the Chinese government and the Chinese transplantation community. The year 2015 marked a historic turning point when voluntary donations from Chinese citizens became the sole legitimate source for organ transplantation. Since 2015, China has gradually established and refined the “Chinese Mode” and “China System” for organ donation and transplantation, fulfilling its political pledge of reform, and has garnered international recognition, and fostered a social culture which promotes organ donation. This article reviewed the history of reform on organ donation and transplantation in China, presented a new pattern of establishment of organ donation system in the new era of the country, and the direction of advances in the future.

关键词： Reform Legislation Organ donation Organ transplantation Chinese mode High quality development

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