检索结果-南通市图书馆

AGK2 pre-treatment protects against thioacetamide-induced acute liver failure via regulating the MFN2-PERK axis and ferroptosis signaling pathway

引用

Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 43-51页

作者： Qing-Qi Zhang Qian Chen Pan Cao chun-xia shi Lu-Yi Zhang Lu-Wen Wang Zuo-Jiong Gong Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060China

Background:Acute liver failure(ALF)is an unpredictable and life-threatening critical *** pathological characteristic of ALF is massive necrosis of hepatocytes and lots of inflammatory cells infiltration which may lead to multiple organ ***:Animals were divided into 3 groups,normal,thioacetamide(TAA,ALF model)and TAA+*** L02 cells were divided into 5 groups,normal,TAA,TAA+mitofusin 2(MFN2)-siRNA,TAA+AGK2,and TAA+AGK2+MFN2-siRNA *** liver histology was evaluated with hematoxylin and eosin staining,inositol-requiring enzyme 1(IRE1),activating transcription factor 6β(ATF6β),protein kinase R(PKR)-like endoplasmic reticulum kinase(PERK)and phosphorylated-PERK(p-PERK).C/EBP homologous protein(CHOP),reactive oxygen species(ROS),MFN2 and glutathione peroxidase 4(GPX4)were measured with Western blotting,and cell viability and liver chemistry were also *** endoplasmic reticulum membranes(MAMs)were measured by ***:The liver tissue in the ALF group had massive inflammatory cell infiltration and hepatocytes necrosis,which were reduced by AGK2 *** comparison to the normal group,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+in the TAA-induced ALF model group were significantly increased,which were decreased by AGK2 *** levels of MFN2 and GPX4 were decreased in TAA-induced mice compared with the normal group,which were enhanced by AGK2 *** with the TAA-induced L02 cell,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+were further increased and levels of MFN2 and GPX4 were decreased in the MFN2-siRNA ***2 pre-treatment decreased the apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+and enhanced the protein expression of MFN2 and GPX4 in MFN2-siRNA treated L02 *** observation showed that level of MAMs was promoted in the AGK2 pre-treatment group when compared with the TAA-induced gr

关键词： SIRT2 inhibitor AGK2 Acute liver failure MFN2 Ferroptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1α

引用

World Journal of Gastroenterology 2022年第17期28卷 1798-1813页

作者： Pan Cao Qian Chen chun-xia shi Lu-Wen Wang Zuo-Jiong Gong Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina

BACKGROUND The occurrence and development of acute liver failure(ALF)is closely related to a series of inflammatory reactions,such as the production of reactive oxygen species(ROS).Hypoxia inducible factor 1α(HIF-1α)is a key factor that regulates oxygen homeostasis and redox,and the stability of HIF-1αis related to the ROS level regulated by Sirtuin(Sirt)*** activation of Sirt1 will lead to a powerful antioxidant defense system and therapeutic effects in liver ***,little is known about the relationship between HIF-1αand Sirt1 in the process of ALF and the molecular *** To investigate whether HIF-1αmay be a target of Sirt1 deacetylation and what the effects on ALF *** Mice were administrated lipopolysaccharide(LPS)/D-gal and exposed to hypoxic conditions as animal model,and resveratrol was used as an activator of *** cellular model was established with L02 cells stimulated by LPS.N-acetyl-Lcysteine was used to remove ROS,and the expression of Sirt1 was inhibited by *** blotting was used to detect Sirt1 and HIF-1αactivity and related protein *** possible signaling pathways involved were analyzed by immunofluorescent staining,co-immunoprecipitation,dihydroethidium staining,and Western *** Compared with mice stimulated with LPS alone,the expression of Sirt1 decreased,the level of HIF-1αacetylation increased in hypoxic mice,and the levels of carbonic anhydrase 9 and Bcl-2-adenovirus E1B interacting protein 3 increased significantly,which was regulated by HIF-1α,indicating an increase of HIF-1α*** hypoxia,the down-regulation of Sirt1 activated and acetylated HIF-1αin L02 *** inhibition of Sirt1 significantly aggravated this effect and the massive production of *** regulation of ROS was partly through peroxisome proliferatoractivated receptor alpha or AMP-activated protein ***,a Sirt1 activator,effectively relieved ALF aggravated by hypoxia,the production

关键词： Acute liver failure Deacetylation Hypoxia inducible factor 1α Reactive oxygen species Sirtuin1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sulforaphane,an NRF2 agonist,alleviates ferroptosis in acute liver failure by regulating HDAC6 activity

引用

结合医学学报:英文版 2023年第5期21卷 464-473页

作者： Yan-qiong Zhang chun-xia shi Dan-mei Zhang Lu-yi Zhang Lu-wen Wang Zuo-jiong Gong Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina

Objective Acute liver failure(ALF)is characterized by severe liver dysfunction,rapid progression and high mortality and is difficult to *** have found that sulforaphane(SFN),a nuclear factor E2-related factor 2(NRF2)agonist,has anti-inflammatory,antioxidant and anticancer effects,and has certain protective effects on neurodegenerative diseases,cancer and liver *** paper aimed to explore the protective effect of SFN in ALF and it possible mechanisms of *** Lipopolysaccharide and D-galactosamine were used to induce liver injury in vitro and in ***2 agonist SFN and histone deacetylase 6(HDAC6)inhibitor ACY1215 were used to observe the protective effect and possible mechanisms of SFN in ALF,*** viability,lactate dehydrogenase(LDH),Fe2+,glutathione(GSH)and malondialdehyde(MDA)were *** expression of HDAC6,NRF2,glutathione peroxidase 4(GPX4),acyl-CoA synthetase long-chain family member 4(ACSL4)and solute carrier family 7 member 11(SLC7A11)were detected by Western blotting and *** Our results show that NRF2 was activated by ***,Fe2+,MDA and ACSL4 were downregulated,while GSH,GPX4 and SLC7A11 were upregulated by SFN in vitro and in vivo,indicating the inhibitory effect of SFN on ***,HDAC6 expression was decreased in the SFN group,indicating that SFN could downregulate the expression of HDAC6 in *** using the HDAC6 inhibitor,ACY1215,SFN further reduced HDAC6 expression and inhibited ferroptosis,indicating that SFN may inhibit ferroptosis by regulating HDAC6 *** SFN has a protective effect on ALF,and the mechanism may include reduction of ferroptosis through the regulation of HDAC6.

关键词： Acuteliverfailure Sulforaphane NRF2 HDAC6 Ferroptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bladder perforation injury after percutaneous peritoneal dialysis catheterization:A case report

引用

World Journal of Clinical Cases 2022年第20期10卷 7054-7059页

作者： chun-xia shi Zhong-Xin Li Hai-Tao Sun Wu-Qing Sun Yu Ji Shu-Jing Jia Department of Nephrology Beijing Luhe Hospital Affiliated to Capital Medical UniversityBeijing 101149China Department of Medicine Beijing Shuangqiao HospitalBeijing 100121China

BACKGROUND Insertion of a catheter into the bladder is a rare complication of peritoneal dialysis(PD),and is mainly related to surgical *** paper reports a case of bladder perforation that was caused by percutaneous PD *** SUMMARY A 64-year-old man underwent percutaneous PD catheterization for end-stage renal *** the second day after the operation,urgent urination and gross hematuria *** showed the presence of red and white blood *** anti-infective treatment was *** the third day after the operation,urgent urination occurred during PD *** showed that the PD catheter was located in the bladder,and subsequent computed tomography(CT)showed that the PD catheter moved through the anterior wall into the *** PD catheter was withdrawn from the bladder and catheterization was *** CT on the fourth day after the operation showed that the PD catheter was removed from the bladder,but there was poor catheter *** PD catheter was removed and the patient was changed to *** cystography showed that the bladder healed well and the patient was discharged 14 d after the *** Bladder perforation injury should be considered and treated timeously in case of bladder irritation during and after percutaneous PD *** use of Doppler ultrasound and other related technologies may reduce the incidence of such complications.

关键词： Bladder perforation Peritoneal dialysis Percutaneous peritoneal dialysis catheterization Peritoneal dialysis catheter dysfunction Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A modified smoothed particle hydrodynamics method considering residual stress for simulating failure and its application in layered rock mass

引用

Journal of Mountain Science 2024年第6期21卷 2091-2112页

作者： xia Chengzhi shi Zhenming KOU Huanjia Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai 200092China Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education Tongji UniversityShanghai 200092China

Residual strength is an indispensable factor in evaluating rock fracture,yet the current Smoothed Particle Hydrodynamics(SPH)framework rarely considers its influence when simulating *** improved cracking strategy considering residual stress in the base bond SPH method was proposed to simulate failures in layered rocks and slopes and verified by experimental results and other simulation methods(i.e.,the discrete element method).Modified Mohr–Coulomb failure criterion was applied to distinguish the mixed failure of tensile and *** fracture markψwas introduced to improve the kernel function after tensile damage,and the calculation of residual stress after the damage was derived after shear *** simulations were carried out to evaluate its performance under different stress and scale conditions and to verify its effectiveness in realistically reproducing crack initiation and propagation and coalescence,even fracture and *** results indicate that the improved cracking strategy precisely captures the fracture and failure pattern in layered rocks and rock *** residual stress of brittle tock is correctly captured by the improved SPH *** improved SPH method that considers residual strength shows an approximately 13%improvement in accuracy for the safety factor of anti-dip layered slopes compared to the method that does not consider residual strength,as validated against analytical *** infer that the improved SPH method is effective and shows promise for applications to continuous and discontinuous rock masses.

关键词： Smoothed particle hydrodynamics Cracking strategy Residual stress Layered rock Crack propagation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Buckling optimization of curvilinear fiber-reinforced composite structures using a parametric level set method

引用

Frontiers of Mechanical Engineering 2024年第1期19卷 149-160页

作者： Ye TIAN Tielin shi Qi xia State Key Laboratory of Intelligent Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China

Owing to their excellent performance and large design space,curvilinear fiber-reinforced composite structures have gained considerable attention in engineering fields such as aerospace and *** addition to the stiffness and strength of such structures,their stability also needs to be taken into account in the *** study proposes a level-set-based optimization framework for maximizing the buckling load of curvilinear fiber-reinforced composite *** the proposed method,the contours of the level set function are used to represent fiber *** a composite laminate with a certain number of layers,one level set function is defined by radial basis functions and expansion coefficients for each ***,the fiber angle at an arbitrary point is the tangent orientation of the contour through this *** the finite element of buckling,the stiffness and geometry matrices of an element are related to the fiber angle at the element *** study considers the parallelism constraint for fiber *** the sensitivity calculation of the objective and constraint functions,the method of moving asymptotes is utilized to iteratively update all the expansion coefficients regarded as design *** numerical examples under different boundary conditions are given to validate the proposed *** show that the optimized curved fiber paths tend to be parallel and equidistant regardless of whether the composite laminates contain holes or ***,the buckling resistance of the final design is significantly improved.

关键词： buckling optimization curvilinear fiber composite structure level set method manufacturing constraint

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An M-VCUT level set-based data-driven model of microstructures and optimization of two-scale structures

引用

Frontiers of Mechanical Engineering 2024年第4期19卷 73-86页

作者： Minjie SHAO Tielin shi Qi xia State Key Laboratory of Intelligent Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China

The optimization of two-scale structures can adapt to the different needs of materials in various regions by reasonably arranging different microstructures at the macro scale,thereby considerably improving structural ***,a multiple variable cutting(M-VCUT)level set-based data-driven model of microstructures is presented,and a method based on this model is proposed for the optimal design of two-scale *** geometry of the microstructure is described using the M-VCUT level set method,and the effective mechanical properties of microstructures are computed by the homogenization ***,a database of microstructures containing their geometric and mechanical parameters is *** two sets of parameters are adopted as input and output datasets,and a mapping relationship between the two datasets is established to build the data-driven model of *** the optimization of two-scale structures,the data-driven model is used for macroscale finite element and sensitivity *** efficiency of the analysis and optimization of two-scale structures is improved because the computational costs of invoking such a data-driven model are much smaller than those of homogenization.

关键词： two-scale structure structural optimization M-VCUT level set homogenization radial basis function data-driven

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Promotion effects of salt-alkali on ammonia volatilization in a coastal soil

引用

Pedosphere 2024年第1期34卷 191-199页

作者： Zhenqi shi Dongli SHE Yongchun PAN Yongqiu xia College of Agricultural Science and Engineering Hohai UniversityNanjing 210098China Jiangsu Province Engineering Research Center for Agricultural Soil-Water Efficient Utilization Carbon Sequestration and Emission ReductionNanjing 210098China Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China

Coastal ecosystems are highly susceptible to salt-related problems due to their formation process and geographical location. As such ecosystems are the most accessible land resources on Earth, clarifying and quantifying the effects of salt-alkali conditions on N concentration and ammonia(NH_(3)) volatilization are pivotal for promoting coastal agricultural productivity. The challenge in establishing this effect is to determine how salt-alkali conditions impact NH_(3) volatilization through direct or indirect interactions. An incubation experiment using a coastal soil from a paddy farmland, combined with the structural equation modeling(SEM) method, was conducted to reveal the net effects of salt-alkali on NH_(3) volatilization and the role of environmental and microbial factors in mutual interaction networks. The specific experimental design consisted of four salt treatments(S1, S2, S3, and S4: 1‰, 3‰, 8‰, and 15‰ NaCl by mass of soil, respectively), four alkaline treatments(A1, A2, A3, and A4: 0.5‰, 1‰, 3‰, and 8‰ NaHCO_(3)by mass of soil, respectively) and a control without NaCl or NaHCO_(3) addition(CK), and each treatment had three urea concentrations(N1, N2, and N3: 0.05, 0.10, and 0.15 g N kg^(-1) soil,respectively) and three replicates. At the N1, N2, and N3 levels, NH_(3)volatilization increased by 9.31%–34.98%, 3.07%–26.92%, and 2.99%–43.61% as the NaCl concentration increased from 1‰ to 15‰, respectively, compared with CK. With an increase in the NaHCO_(3)concentration from 0.5‰ to 8‰, NH_(3) volatilization increased by 8.36%–56.46%, 5.49%–30.10%, and 30.72%–73.18% at the N1, N2, and N3 levels, respectively, compared with CK. According to the SEM method, salinity and alkalinity had positive direct effects on NH_(3)volatilization, with standardized path coefficients of 0.40 and 0.19, *** the total effects(net positive and negative effects) in the SEM results, alkalinity had a greater influence than salinity(total standardized coefficien

关键词： alkalinity coastal ecosystem NH_(3)emission N-transforming microbe salinity structural equation modeling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Time-resolved ultra-small-angle X-ray scattering beamline(BL10U1)at SSRF

引用

Nuclear Science and Techniques 2024年第2期35卷 6-19页

作者： Wen-Qiang Hua chun-Ming Yang Ping Zhou Feng Tian Jin-You Lin Yu-Zhu Wang xiao-Yun Li xia-Ran Miao chun-xia Hong Qiu-shi Huang Xin-Tong Zhao Yong-Feng Men Jie Wang Xing-Yu Gao Xiu-Hong Li Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China Key Laboratory of Advanced Micro Structural Materials MOEInstitute of Precision Optical EngineeringSchool of Physics Science and EngineeringTongji UniversityShanghai 200092China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

The construction of a new beamline,BL10U1,was completed at the Shanghai synchrotron radiation facility in *** multipurpose beamline was designed to provide X-ray scattering techniques such as ultra-small-angle X-ray scattering(USAXS),small-angle X-ray scattering(SAXS),wide-angle X-ray scattering,and microfocus SAXS(μSAXS)for a broad user *** realize fast time-resolved USAXS experiments,the beamline adopted an in-vacuum undulator with a total length of 1.6 m as the photon *** in-house cryogenic-cooled double multilayer monochromator was installed to deliver a photon flux of approximately 10^(13) photons/s at a photon energy of 10 *** three-year successful operation of this beamline demonstrated that the monochromator operated smoothly,as ***10U1 has three end stations in succession:USAXS end station,μSAXS end station,and end station for industrial *** minimum scattering vector q~0.0042 nm^(-1) at 10 keV can be achieved at the USAXS end station equipped with a 28 m-long and 1.8 m-diameter vacuum flight *** theμSAXS end station,a beam spot of less than 10×8μm was achieved for micro-SAXS *** contrast,in situ experimental instruments up to 5 m high and 8 m wide can be mounted at the industrial application end station,which offers industrial scientists the opportunity to use their large industrial ***10U1 opens up a new capability to investigate phenomena such as non-equilibrium and dynamic processes of materials with a wide length scale from angstroms to micrometers with millisecond time *** this paper,we also report beamline design considerations and commissioning results.

关键词： Synchrotron radiation Ultra-small-angle X-ray scattering Micro small-angle X-ray scattering USAXS Time resolved μSAXS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Vortex identification based on the Liutex method and its effect on fish passage upstream

引用

Journal of Hydrodynamics 2024年第1期36卷 130-141页

作者： chun-ying Shen Rui-guo Yang xiao-tao shi Ming-ming Wang shi-hua He Faculty of Electrical Power Engineering Kunming University of Science and TechnologyKunming650500China Hubei International Science and Technology Cooperation Base of Fish Passage China Three Gorges UniversityYichang443002China Shanghai Investigation Design and Research Institute Co.LtShanghai200434China

Fishway research is important for mitigating the fragmentation of river habitats caused by hydraulic *** vertical slit fishway is a broadly used fishway type because of its high efficiency and adaptability to water ***,the resulting vortex current disrupts the fish passage hence directly affecting fish *** study aims to accurately capture the vortex structure in the fishway and analyze the effect of vortex elements(vortex structure,vortex intensity,etc.)on *** conducted an analysis of the 3-D current flow field in the fishway through the utilization of an experimental model and the large eddy simulation(LES)***,we captured the vortex information in the fishway at different flow rates using the Liutex vortex identification method and investigated the effect of the vortex on fish *** results revealed that the structures inside the fishway pool occupy most of the room,however,the areas with higher vortex strength were primarily located in the vortex near the vertical seam and the mainstream,the vortex strength inside the fishway gradually increases with increasing flow,suppressing fish *** experienced significantly increased resistance when encountering strong *** suggests that the vortex may act as a physical barrier to fish *** findings highlight the potential negative effects of vortex on fish movement and reiterate the importance of understanding vortex dynamics for aquatic environmental *** an effective tool for identifying vortices in fluid flow,the Liutex method demonstrates features of vortex within the fishway,thereby providing important insights into the interaction between fluid dynamics and aquatic organisms.

关键词： Vertical slit fishway vortex identification method Liutex large eddy simulation(LES) fish experiment computational fluid dynamics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：