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Effects of plant density on cotton yield components and quality

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Journal of Integrative Agriculture 2016年第7期15卷 1469-1479页

作者： ZHI Xiao-yu HAN ying-chun LI Ya-bing WANG Guo-ping DU Wen-li LI Xiao-xin MAO Shu-chun FENG Lu Institute of Cotton Research Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology

Yield and ifber quality of cotton even varies within locules in a bol, but it is not clear how yield components and quality parameters are altered across seed positions of a locule （SPL）. A ifeld experiment was arranged in a split plot design with transgenic insect resistant Bt （Bacilus thuringiensis） cotton hybrid cultivar CRI75 and conventional cultivar SCRC28 as the main plots, and three plant densities （15000, 51000 and 87000 plants ha–1） as the subplots in 2012 and 2013 at Anyang, Henan Province, China. Cotton was hand harvested by node and fruiting position, and then seeds of the ifrst fruiting posi-tion bols from nodes 6–10 were separated by SPL. The effects of plant density on lint yield, ifber quality, especialy across SPL were determined. It was showed that plant densities of 51000 and 87000 plants ha–1 increased lint yield by 61.3 and 65.3% in 2012 and 17.8 and 15.5% in 2013 relative to low plant density （15000 plants ha–1）, however, no signiifcant differ-ence was observed between 51000 and 87000 plants ha–1. The number of bols （bol density） increased while bol weight decreased as plant density raised, and no signiifcant changes occured in lint percentage in 2013 but increased with plant density in 2012. The number of bols in upper nodes and distal fruiting positions, the number of seeds per bol, seed area （SA） and seed vigor index increased with decreasing plant density. Seed area was found to be greater from the base to the middle compared to the apex of a locule. Mote frequency （MF） increased as plant density increased, and ifber quality was the best at the middle of the locule regardless of plant density. As the number of ifbers per seed area is geneticaly determined, adjusting plant density to produce more seeds and greater seed area can be a potentialy promising alternative to improve lint yield in cotton. These ifndings might be of great importantance to cotton breeding and ifled management.

关键词： cotton plant density yield quality seed position

Affinity peptide developed by phage display selection for targeting gastric cancer

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World Journal of Gastroenterology 2012年第17期18卷 2053-2060页

作者： Wen-Jie Zhang Yan-Xia Sui Arun Budha Jian-Bao Zheng Xue-Jun Sun ying-chun Hou Thomas D Wang Shao-ying Lu Department of General Surgery The First Affiliated Hospital of Medical School Xian JiaotongUniversity Xi＇an 710061 Shaanxi Province China The Third Hospital of Chengdu Chengdu610031 Sichuan Province China Shaanxi Normal University Xi＇an 710061Shaanxi Province China Department of Medicine Division of Gastroenterology and Hepatology School of Medicine Universityof Michigan Ann Arbor MI 48109 United States

AIM:To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric ***:A peptide screen was performed by biopanning the PhD-12 phage display library,clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer *** binding of selected peptides was confirmed by bound phage counts,enzyme-linked immunosorbent assay,competitive inhibition,fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer ***:Approximately 92.8% of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normal-appearing gastric *** the third round of positive screening,the peptide sequence AADNAKTKSFPV(AAD) appeared in 25%(12/48) of the analyzed *** the control peptide,these values were 6.8 ± 2.3,5.1 ± 1.7,3.5 ± 2.1,4.6 ± 1.9 and 1.1 ± 0.5,*** values for AAD peptide were statistically signif icant(P < 0.01) for gastric cancer as compared with other histological classif ications and control ***:A novel peptide is discovered to have a specific binding activity to gastric cancer,and can be used to distinguish neoplastic from normal gastric mucosa,demonstrating the potential for early cancer detection on endoscopy.

关键词： Gastric cancer Peptide Phage library Molecular imaging Early detection Immunohistochemistry Enzyme-linked immunosorbent assay

Effects of integrin-targeted photodynamic therapy on pancreatic carcinoma cell

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World Journal of Gastroenterology 2013年第39期19卷 6559-6567页

作者： Min Zhou Qian-Wen Ni Shan-ying Yang chun-ying Qu Peng-Cheng Zhao Jian-Cheng Zhang Lei-Ming Xu Digestive Endoscopic Diagnosis and Treatment Center Xinhua HospitalSchool of MedicineShanghai Jiaotong University Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition School of Materials Science and Engneering Shanghai University

AIM:To investigate the effects of photodynamic therapy with quantum dots-arginine-glycine-aspartic acid(RGD)probe as photosensitizer on the proliferation and apoptosis of pancreatic carcinoma ***:Construction of quantum dots-RGD probe as photosensitizer for integrin-targeted photodynamic therapy was *** cells were treated with photodynamic therapy(PDT),the proliferation of SW1990 cells were measured by methyl thiazolyl tetrazolium *** changes,cell cycle retardance and apoptosis were observed under fluoroscope and flow *** expression of myeloid cell leukemia-1(Mcl-1),protein kinase B(Akt)and tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)mRNA were detected by reverse transcriptionpolymerase chain *** amount of reactive oxygen species were also evaluated by fluorescence ***:The photodynamic therapy with quantum dots-RGD probe as photosensitizer significantly inhibited cell proliferation(P<0.01).Apoptotic cells and morphologic changes could be found under optical *** FCM revealed PDT group had more significant cell apoptosis rate compared to control cells(F=130.617,P<0.01)and cell cycle G0/G1and S retardance(P<0.05)compared to control *** expression of Mcl-1 and Akt mRNA were down-regulated,while expression of TRAIL mRNA was up-regulated after cells treated with *** group had more significant number of cells producing reactive oxygen species compared to control cells(F=3262.559,P<0.01).CONCLUSION:The photodynamic therapy with quantum dots-RGD probe as photosensitizer significantly inhibits cell proliferation and increases apoptosis in SW1990 cells.

关键词： Pancreatic carcinoma Targeted probe Photodynamic therapy Apoptosis Reactive oxygen species

Early Triassic Legoupil Formation in Schmidt Peninsula,Antarctic Peninsula:Provenance and Depositional Settings

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Journal of Earth Science 2024年第2期35卷 317-331页

作者： Chao Zhang ying-chun Cui Chen-Guang Liu Fang-Hua Cui Lu-Yuan Wang Wei-Qiang Zhang School of Resources and Environmental Engineering Shandong University of TechnologyZibo255049China MNR Key Laboratory for Polar Science Polar Research Institute of ChinaShanghai201209China Key Laboratory of Marine Sedimentology and Environmental Geology First Institute of OceanographyState Oceanic AdministrationQingdao266061China Laboratory for Marine Geology Qingdao National Laboratory for Marine Science and TechnologyQingdao266061China Key Laboratory of Mineral Resources Evaluation in Northeast Asia Ministry of Land and ResourcesChangchun130061China

Geochemical compositions can be used to determine the tectonic setting of sedimentary basins,while where the link of source to sink is no longer preserved,detrital zircon age patterns can aid in resolving the original basin *** metasedimentary Legoupil Formation,located at Cape Legoupil and the Schmidt Peninsula,could give a hint for the tectonic evolution of Antarctic *** this contribution,we constrain the sedimentary provenance of the Legoupil Formation through geochemistry and detrital zircon U-Pb *** petrography and geochemical features indicate that the provenance of the Legoupil Formation could be felsic *** zircon grains record a steady supply of Permian and Ordovician material into the Legoupil *** youngest concordant zircon ages of 262 Ma suggest that the depositional time of Legoupil Formation is no older than Late *** detrital zircon age spectrum of Legoupil Formation suggests that the Legoupil Formation sediments should be derived from regional sources endemic to western Gondwana prior to its *** with the previous studies,geochemistry and detrital zircons reflect an active continental margin tectonic setting and the detrital zircon spectra of Legoupil Formation are similar to the ones deposited in forearc tectonic setting.

关键词： Schmidt Peninsula Legoupil Formation Trinity Peninsula Group detrital zircons Antarctic Peninsula geochemistry

The prevalence of transfusion transmitted virus infection in blood donors

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World Journal of Gastroenterology 2000年第2期6卷 268-270页

作者： Cheng Hui Huang Yu Sen Zhou Ru Guang Chen chun ying Xie Hai Tao Wang Department of Blood Transfusion Research Shenzhen Baoan Blood CenterShenzhen 518101Guangdong ProvinceChina Department of Hepatitis Virus Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Department of Molecular Biology Shenzhen Baoan People’s HospitalShenzhen 518101Guangdong ProvinceChina

INIRODUCIIONA newly discovered DNA virus,transfusiontransmitted virus (TTV),was reported as a cause ofpost-transfusion hepatitis of unknown etiology *** order to investigate TTV prevalence insouthern China,a study was carried out amongblood donors,patients with liver diseases andhemodialysis to determine the epidemiologicalcharateristics.

关键词： transfusion transmitted virus infection blood donors liver diseases hemodialysis

Synthesis and electrochemical properties of a chiral silyl-substituted tetrathiafulvalene derivative

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Chinese Chemical Letters 2014年第4期25卷 579-582页

作者： Guo-Qi Liang Zhong-Bao Zhang Hong-Qi Li Ya-Ping Wang chun-ying Xian College of Chemistry Chemical Engineering and BiotechnologyDonghua University

A new chiral tetrathiafulvalene （TTF） derivative and related silyl-substituted 1,3-dithiole-2-（thi）one compounds were synthesized and characterized by 1H NMR, 13C NMR, MS and IR spectra. Single crystal structure of the silyl-substituted 1,3-dithiole-2-one revealed the high degree of conjugation of the five- membered ring moiety in the compound. The electrochemical properties of the new TTF derivative were studied by cyclic voltammetry and the results indicated that the electron-donating ability of the chiral TI＂F derivative was similar to that of BEDT-TTF. The AE value for the new TTF derivative was smaller than those for TTF and BEDT-TrF, indicative of decreased Coulombic repulsion in the dicationic redox state. Formation of charge-transfer （CT） complex between the new donor and electron acceptor 2,3-dichloro-5,6-dicvano-1,4-benzocluinone （DDO） was demonstrated.

关键词： Tetrathiafulvalene Chiral Silyl Electrochemistry

La^(3+) Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence

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Chinese Chemical Letters 2000年第8期11卷 725-726页

作者： Rong CHEN, Pin YANG, chun ying WEI (Institute of Molecular Science, Shanxi University. Taiyuan 030006) Institute of Molecular Science Shanxi University Taiyuan 030006 China

Binding of La3+ to Fura-2 can change 340/380 nm fluorescence intensity ratio. Whether La3+ cross ventricular cell membrane was detected by this fluorescent probe technique. Fura-2 loaded isolated guinea pig ventricular cells were exposed to 0.01-0.1 mM extracellular Lanthanum ion concentration, 340nm/380 nm fluorescence ratio was not changed. Using calcium channel agonist BAY K8644, KCL (35mM) depolarization to open the voltage-dependent calcium channel (VDCC); Adrenoceptor agonist to excite adrenoceptor, 340/380 ratio was not changed, suggesting that La3+can not enter guinea pig ventricular cells in this case.

关键词： Ventricular cells adrenaline La3+ transmembrane Fura-2 BAY K8644.

Synthesis of Novel Quaternary Selenide K_2CdSnSe_4

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Chemical Research in Chinese Universities 2010年第4期26卷 514-516页

作者： ZHANG Li-dan HAN chun-ying FENG Xiu-ling WEI Yong-ge GUO Hong-you College of Sciences Beoing University of Chemical Technology Beijing 100029 P. R. China Department of Chemistry Huaihua Institute Huaihua 418008 P. R. China College of Chemistry and Molecular Engineering Peking University Beoing 100871 P. R. China

A novel quaternary metal selenide, K2CdSnSe4, was synthesized via a molten-salt reaction at 550 ℃. The compound has a one-dimensional structure containing a chain of ∞^1[CdSnSe4]^2- anions separated by K^＋ cations. The crystal belongs to the space group I-4^-2m, with the unit cell parameters a=0.80298（11） nm, b=0.80298（11） nm, c=0.69217（14） nm, α=β=γ=90°. A reflectance spectroscopy study reveals the nature of the semiconductor with an energy gap of 1.7 eV for the compound and it has a strong optical absorotion in a range of 2--5 eV.

关键词： Quatemary metal selenide Solid state synthesis Crystal structure

Effect of intrauterine perfusion of granular leukocyte-colony stimulating factor on the outcome of frozen embryo transfer

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World Journal of Clinical Cases 2021年第30期9卷 9038-9049页

作者： ying-chun Zhu Yan-Xin Sun Xiao-Yue Shen Yue Jiang Jing-Yu Liu Reproductive Medicine Center The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008Jiangsu ProvinceChina

BACKGROUND Treatment of thin endometrium with granular leukocyte-colony stimulating factor(G-CSF)remains *** To investigate the effect of G-CSF on the outcome of frozen embryo transfer in patients with thin *** A retrospective propensity score matching(PSM)study was performed to assess patients administered frozen embryo transfer at the Reproductive Medicine Center of the Affiliated Drum Tower Hospital of Nanjing University Medical School,in *** patients were divided into G-CSF intrauterine perfusion(G-CSF)and non-G-CSF groups,and clinical pregnancy,implantation,ectopic pregnancy,and early abortion rates between the two groups were *** Before PSM,372 cycles were enrolled,including 242 and 130 cycles in the G-CSF and non-G-CSF groups,***(34.23±5.76 vs 32.99±5.59 years;P=0.047)and the blastula/cleavage stage embryo ratio(0.68 vs 0.37;P=0.011)were significantly elevated in the G-CSF group compared with the non-G-CSF group;however,clinical pregnancy(46.28%vs 51.54%;P=0.371)and embryo implantation(35.21%vs 35.65%;P=0.910)rates were similar in both *** PSM by age and blastula/cleavage stage embryo ratio,244 cycles were included(122 cases each in the G-CSF and non-G-CSF groups).The clinical pregnancy(50.82%vs 48.36%;P=0.701)and embryo implantation(37.38%vs 34.11%;P=0.480)remained similar in both *** Intrauterine infusion of G-CSF does not improve the clinical outcome of frozen embryo transfer in patients with thin endometrium.

关键词： Thin endometrium Granular leukocyte-colony stimulating factor Intrauterine perfusion Frozen embryo transfer