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燃烧-催化氧化法治理有机废气

化学工业与工程技术 2000年第2期21卷 11-13页

作者：刘治雄张晓春张浩黄建明南化集团研究院南京210048

介绍了采用燃油加热废气的催化氧化法治理大气量有机废气 (含丁酮、丁醇和乙醇 )的技术和装置。阐述了火焰加热对催化氧化反应的促进作用。

关键词：废气治理催化氧化有机废气燃烧

Effects of grafting cell penetrate peptide and RGD on endocytosis and biological effects of Mg-CaPNPs-CKIP-1 siRNA carrier system in vitro

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Journal of Central South University 2021年第5期28卷 1291-1304页

作者： YI Man-fei CHEN Liang-jian HE Hui-li SHI Lei SHAO chun-sheng ZHANG Bo Department of Stomatology the Third Xiangya HospitalCentral South UniversityChangsha 410013China State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China

Calcium phosphate nanoparticles(CaPNPs)have good biocompatibility as gene carriers;however,CaPNPs typically exhibit a low transfection *** penetrate peptide(tat)can increase the uptake of nanoparticles but is limited by its *** adhesion peptide adhesion peptide on carriers can enhance their *** Plekho1 gene encodes casein kinase-2 interacting protein-1(CKIP-1),which can negatively regulate osteogenic *** on the above,we produced a Mg-CaPNPs-RGD-tat-CKIP-1 siRNA carrier system via hydrothermal synthesis,silanization and *** effects of this carrier system on cell endocytosis and biological effects were evaluated by cell culture in *** results demonstrate that CaPNPs with 7%Mg(60 nm particle size,short rod shape and good dispersion)were suitable for use as gene *** carrier system boosted the endocytosis of MG63 cells and was helpful for promoting the differentiation of osteoblasts,and the dual-ligand system possessed a synergistic *** findings of this study show the tremendous potential of the Mg-CaPNPs-RGD-tat-CKIP-1 siRNA carrier system for efficient delivery into cells and osteogenesis inducement.

关键词： calcium phosphate nanoparticles adhesion peptide cell penetrate peptide endocytosis siRNA

钍尘职业暴露工人外周血淋巴细胞染色体畸变的研究

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中华放射医学与防护杂志 2008年第3期28卷 242-245页

作者：张伟王春燕吕慧敏贾柯君刘玉飞张翠兰郝述霞苏旭中国疾病预防控制中心辐射防护与核安全医学所北京100088 包钢劳动卫生职业病防治研究所

目的研究含钍稀土混合粉尘对职业暴露人员外周血淋巴细胞染色体畸变的影响。方法采用常规吉姆萨染色方法分析53名钍尘作业工人及58名非接尘工人（对照组）的染色体非稳定性畸变，荧光原位杂交方法分析10名钍尘作业工人及11名非接尘工人... 详细信息

目的研究含钍稀土混合粉尘对职业暴露人员外周血淋巴细胞染色体畸变的影响。方法采用常规吉姆萨染色方法分析53名钍尘作业工人及58名非接尘工人（对照组）的染色体非稳定性畸变，荧光原位杂交方法分析10名钍尘作业工人及11名非接尘工人的染色体稳定性畸变。结果接尘组的畸变细胞、双着丝粒＋环和总畸变率显著高于对照组（Χ^2=4．12，P〈0．05；Χ^2=5．07，P〈0．05；Χ^2=7．09，P〈0．01）。无着丝粒的畸变率在接尘组与对照组间差异无统计学意义。接尘组的易位率（4．5±2．0）与对照组的易位率（3．8±1．7）比较差异无统计学意义（Z=-0．566，P〉0．05）。结论长期职业暴露于含钍稀土混合粉尘能够增加染色体的非稳定性畸变，未观察到易位率增加。

关键词：职业暴露钍稀土混合粉尘淋巴细胞染色体畸变

An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages

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Fundamental Research 2022年第1期2卷 2-13页

作者： Meiling Piao Pan Li Xiaomin Zeng Xi-Wen Wang Lan Kang Jinsong Zhang Yifan Wei Shaojun Zhangas Lei Tang Jianghui Zhu chun Kit Kwok Xiaoyu Hu Qiangfeng Cliff Zhang MOE Key Laboratory of Bioinformatics Center for Synthetic and Systems BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Beijing Advanced Innovation Center for Structural Biology&Frontier Research Center for Biological Structure School of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for Life Sciences Beijing 100084China Institute for Immunology and School of Medicine Tsinghua UniversityBeijingChina Beijing Key Laboratory for Immunological Research on Chronic Diseases Tsinghua-Peking Center for Life SciencesTsinghua UniversityBeijingChina department of Chemistry City University of Hong KongTat Chee AvenueKowloon TongHong KongChina Shenzhen Research Institute of City University of Hong Kong ShenzhenChina

To enable diverse functions and precise regulation,an RNA sequence often folds into complex yet distinct structures in different cellular *** RNA in its native environment is essential to uncovering RNA structures of biological ***,current methods generally require large amounts of input RNA and are challenging for physiologically relevant ***,we report smartSHAPE,a new RNA structure probing method that requires very low amounts of RNA input due to the largely reduced artefact of probing signals and increased efficiency of library *** smartSHAPE,we showcased the profiling of the RNA structure landscape of mouse intestinal macrophages upon inflammation,and provided evidence that RNA conformational changes regulate immune *** results demonstrate that smartSHAPE can greatly expand the scope of RNA structure-based investigations in practical biological systems,and also provide a research paradigm for the study of post-transcriptional regulation.

关键词： RNA structure probing method Low-input RNA structure element Macrophage RNA structure RNA structurome

Study on tat Mediated Magnetic Nanoparticles Having Composite Targeting Function

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Chinese Journal of Biomedical Engineering(English Edition) 2005年第4期14卷 150-155页

作者： YAO Peng HUANG Jie ZHAO Ai-jie KANG chun-sheng CHANG Jin PU Pei-yu Institute of Nanobiotechnology School of Materials Science and Engineering Tianjin University Tianjin 300072China General Hospital Tianjin Medical University Tianjin 300052China

This paper describes a new formulation of magnetic nanoparticles coated by a novel polymer matrix-O-Carboxylmethylated Chitosan (O-CMC) as a drug/gene carrier. The O-CMC magnetic nanoparticles were derivatized with a peptide sequence from the HIV-tat protein and transferrin to improve the translocational property and cellar uptake of the nanoparticles. To evaluate the O-MNPs-tat-Tf as a drug carrier, Methotrexate (MTX) was incorporated as a model drug and MTX-loaded O-MNPs-tat-Tf with an average diameter of 75 nm were prepared and characterized by TEM, AFM and *** cytotoxicity of MTX-loaded O-MNPs-tat-Tf was investigated with C6 cells. The results showed that the MTX-loaded O-MNPs-tat-Tf retained significant antitumor toxicity.

关键词： Magnetic nanoparticles O-carboxylmethylated chitosan tat Transferrin MTX Target Drug delivery system

细胞穿膜肽tat以非融合形式递送蛋白质的能力探究

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药学学报 2023年第8期58卷 2384-2390页

作者：窦佳吉丽娜华子春南京大学生命科学学院医药生物技术国家重点实验室江苏南京210023 江苏省产业技术研究院医药生物技术研究所常州南京大学高新技术研究院江苏常州213164 南京吉芮康生物科技研究院江苏南京210044

穿膜肽tat被证实可用于不同类型药物分子的递送,包括核酸、蛋白质和小分子药物等。tat可以通过共价连接和非共价连接等形式递送分子。但是迄今tat采用非共价连接形式递送蛋白质分子的报道较少,也未有研究比较过融合形式和非融合形式下TA... 详细信息

穿膜肽tat被证实可用于不同类型药物分子的递送,包括核酸、蛋白质和小分子药物等。tat可以通过共价连接和非共价连接等形式递送分子。但是迄今tat采用非共价连接形式递送蛋白质分子的报道较少,也未有研究比较过融合形式和非融合形式下tat递送蛋白质能力的差异。为了探究非融合形式对于tat递送蛋白质能力的影响,本文采用了荧光显微镜观察和流式细胞检测法在细胞水平上探究了tat以非融合形式递送增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)进入人非小细胞肺癌细胞A549的能力,发现其可以穿膜递送EGFP,并且递送能力与tat浓度呈现正相关。同时,本文还表达纯化了tat与绿色荧光蛋白质EGFP的融合蛋白tat-EGFP并考察了其穿膜能力。本文将两种形式的递送效果进行定量对比后发现:当tat以融合蛋白形式递送EGFP时,其递送能力要明显优于tat以非融合形式递送EGFP的能力。本文报道了tat能够以非融合形式递送EGFP,尽管其递送效果有待进一步提高,但是该递送形式在操作便捷性上有着不可比拟的优势,是未来一种值得选择的递送方案。

关键词：细胞穿膜肽 tat 非融合表达递送形式 tat-EGFP 穿膜能力

耻骨直肠肌综合征的排粪造影诊断(附86例分析)

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医学影像学杂志 2006年第1期16卷 59-60页

作者：蔡志春张新毅刘培喜吴凡山东省职业卫生与职业病防治研究院山东济南250002 山东省交通医院山东济南250031

目的:探讨耻骨直肠肌综合征排粪造影的X线征象和临床应用价值。方法:稠钡灌肛,令病人侧坐检查床前排粪桶上,透视下排钡,摄取静坐、提肛、力排及力排后粘膜像,并做多种测量。结果:全部病例不同程度出现下列征象:肛直角不变或变化极小,“... 详细信息

目的:探讨耻骨直肠肌综合征排粪造影的X线征象和临床应用价值。方法:稠钡灌肛,令病人侧坐检查床前排粪桶上,透视下排钡,摄取静坐、提肛、力排及力排后粘膜像,并做多种测量。结果:全部病例不同程度出现下列征象:肛直角不变或变化极小,“搁架”征,钡剂不排或少排,肛管细长。结论:排粪造影可为诊断耻骨直肠肌综合征提供最为客观的主要诊断依据。

关键词：耻骨直肠肌综合征排粪造影肛直角