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Construction and identification of recombinant lentiviral vector containing HIV-1 tat gene and its expression in 293T cells

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The Journal of Biomedical Research 2010年第1期24卷 58-63页

作者： Bingbing Wei Ninghan Feng Feng Zhou chun Lu Jiantang Su Lixin Hua Department of Urology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China Department of Microbtology and Immunology Nanjing Medical University Nanjing 210029 China

Objective： To construct a lentiviral vector expressing HIV-1 tat and identify its expression in 293T cells. Methods： The gene fragment of HIV-1 tatlol was subcloned to lentiviral transfer vector pHAGE-CMV-MCS- IZsGreen, which was named pHAGE-tat. Then the constructed pHAGE-tat was used to co-transfect the packing 293T cells, together with the packaging plasmids pMD2.G and psPAX2. The packaged viral particles designated LV-tat were used to infect the 293T cells and the viral titer was calculated. The expression of HIV-1 tat in 293T cells was confirmed using RT-PCR and western blot. Results： The recombinant lentiviral vector was successfully constructed and could express HIV-1 tat in 293T cells. The virus titer was 5.73×10^6 ifu/ml. Conclusion： The successfully constructed recombinant lentiviral vector makes a strong foundation for further exploring the possible role of HIV-1 tat in the development of prostate cancer.

关键词： prostate cancer HIV-1 tat

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模拟法人之间内部结转价格的制订方法研讨

西安交通大学学报（社会科学版） 2001年第1期21卷 35-38页

作者：吕春晓宋合义西安交通大学陕西西安710049

运用定量分析模型研究了国有企业再造过程中产生的企业内部模拟法人之间的内部结转价值和制订问题 ,提出了运用间接利用分配原则制订内部结构转价格的方法 ,并对这种方法的效果进行了理论分析 ,认为这种方法有利于缓解各利益主体之间的... 详细信息

运用定量分析模型研究了国有企业再造过程中产生的企业内部模拟法人之间的内部结转价值和制订问题 ,提出了运用间接利用分配原则制订内部结构转价格的方法 ,并对这种方法的效果进行了理论分析 ,认为这种方法有利于缓解各利益主体之间的直接矛盾并能兼顾各方面的利益需求。

关键词：企业再造模拟法人内部结转价格生产部门销售部门供应部门

Effects of resistance training on cardiovascular health in non-obese active adolescents

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World Journal of Clinical Pediatrics 2016年第3期5卷 293-300页

作者： Clare chung-Wah Yu Alison Mary McManus Hung-Kwan So Ping Chook chun-Ting Au Albert Martin Li Jack tat-Chi Kam Raymond Chi-Hung So Christopher Wai-Kei Lam Iris Hiu-Shuen Chan Rita Yn-Tz Sung Department of Health and Physical Education the Education University of Hong Kong Institute of Human Performance the University of Hong Kong Centre for Heart Lung and Vascular HealthSchool of Health and Exercise SciencesUniversity of British Columbia Department of Paediatrics the Chinese University of Hong Kong Hong Kong Sports Institute Faculty of Health Sciences and University Hospital Macao University of Science and Technology Department of Chemical Pathology Prince of Wales Hospitalthe Chinese University of Hong Kong

AIM:To determine the benefits of a 10-wk resistance training programme on cardiovascular health in nonobese and active ***:This is a pragmatic randomised controlled *** study was carried out in a Hong Kong Government secondary ***-eight lean and active boys and girls were randomised to either the resistance training group or the control *** in the resistance training group received in-school 10-wk supervised resistance training twice per week,with each session lasting 70 *** outcome measures taken before and after training included brachial endothelial dependent flow-mediated dilation,body composition,fasting serum lipids,fasting glucose and insulin,high sensitive C-reactive protein,24-h ambulatory blood pressure and aerobic ***:The only training related change was in endothelial dependent flow-mediated dilation which increased from 8.5%to 9.8%.A main effect of time and an interaction(P<0.005) indicated that this improvement was a result of the 10-wk resistance *** effects for time(P<0.05) in a number of anthropometric,metabolic and vascular variables were noted;however,there were no significant interactions indicating the change was more likely an outcome of normal growth and development as opposed to a training ***:Ten weeks of resistance training in school appears to have some vascular benefit in active,lean children.

关键词： Strength training Children Cardiometabolic risk factors Endothelial function School-based training program High sensitive C-reactive protein 24-h ambulatory blood pressure Aerobic fitness

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女性Ⅱ型糖尿病患者骨密度的探讨

宁夏医学杂志 2000年第3期22卷 142-143页

作者：刘靖蔡万春解放军第五医院内分泌科宁夏银川750004

目的　探讨女性Ⅱ型糖尿病患者骨密度 (BMD)变化。方法　应用单光子骨矿物密度仪测量了 16 3例女性Ⅱ型糖尿病患者及 192例健康对照者。结果　糖尿病 (DM )组骨量及骨量减少检出率与对照组无显著性差异。年龄、绝经年限与DM患者骨密度BM... 详细信息

目的　探讨女性Ⅱ型糖尿病患者骨密度 (BMD)变化。方法　应用单光子骨矿物密度仪测量了 16 3例女性Ⅱ型糖尿病患者及 192例健康对照者。结果　糖尿病 (DM )组骨量及骨量减少检出率与对照组无显著性差异。年龄、绝经年限与DM患者骨密度BMD呈负相关 (P <0 .0 1)。DM骨量减少组视网膜病变、肾病以及绝经例数显著高于、而血清胰岛素 (INS)水平显著低于骨量正常组 (P <0 .0 1)。结论　提示DM不增加女性BMD下降的风险。

关键词：糖尿病妇女骨密度 Ⅱ型

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粉末活性炭用于反渗透进水预处理的新工艺

水处理技术 2006年第3期32卷 46-48,65页

作者：罗安涛陈晓春孙巍彭雪飞刘时伟北京化工大学化学工程学院北京100029

为了提高反渗透(RO)进水预处理效率,减少膜污染,本文提出一种把粉末活性炭(PAC)应用于反渗透进水预处理的新方法。该法应用粉末活性炭代替颗粒活性炭(GAC),将PAC悬浮流化在料液中;部分PAC在系统中回流;失活PAC进行适时再生。该法解决了... 详细信息

为了提高反渗透(RO)进水预处理效率,减少膜污染,本文提出一种把粉末活性炭(PAC)应用于反渗透进水预处理的新方法。该法应用粉末活性炭代替颗粒活性炭(GAC),将PAC悬浮流化在料液中;部分PAC在系统中回流;失活PAC进行适时再生。该法解决了颗粒活性炭过滤器可能会带来的生物污染问题。特别是采用PAC回流的方法充分利用了PAC的吸附能力,节省了PAC的用量。

关键词：粉末活性炭水处理反渗透

不同生活型绿化植物叶片碳同位素组成的季节特征

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植物生态学报 2010年第2期34卷 151-159页

作者：王玉涛李吉跃刘平陈崇何春霞沈阳农业大学林学院沈阳110161 北京林业大学省部共建森林培育与保护教育部重点实验室北京100083 华南农业大学林学院广州510642

通过测定北京地区不同生活型绿化植物叶片的碳同位素组成(δ13C值),从植物种和生活型两个方面研究植物水分利用效率的自然可变性。结果表明:所测定的75种植物(隶属于35科65属)的叶片的δ13C值变幅,春季为-30.7‰--23.4‰,夏季为-31.5‰-... 详细信息

通过测定北京地区不同生活型绿化植物叶片的碳同位素组成(δ13C值),从植物种和生活型两个方面研究植物水分利用效率的自然可变性。结果表明:所测定的75种植物(隶属于35科65属)的叶片的δ13C值变幅,春季为-30.7‰--23.4‰,夏季为-31.5‰--25.1‰,秋季为-31.4‰--23.9‰;落叶灌木种间差异不显著(p=0.114),而常绿乔木(p=0.005)、落叶乔木(p灌木树种>藤本植物>草本植物、常绿植物>落叶植物的规律。因此,植物种和生活型均会引起植物叶片δ13C值的变化,但δ13C受生活型变化的影响较大,表明不同生活型植物的水分利用效率具有明显差异。

关键词：碳同位素组成绿化植物生活型季节特征水分利用高效率

Improvement of mechanical properties of Sn-58Bi alloy with multi-walled carbon nanotubes

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中国有色金属学会会刊：英文版 2012年第S3期22卷 692-696页

作者： HE Peng Lü Xiao-chun LIN Tie-song LI Hai-xin AN Jing MA Xin FENG Ji-cai ZHANG Yan LI Qi QIAN Yi-yu State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology Department of Materials Science and Engineering Shenzhen Graduate SchoolHarbin Institute of Technology Yik Shing tat Industrial Company Limited CNano Technology (Beijing) Limited

Carbon nanotubes (CNTs) reinforced Sn-58Bi composites were successfully fabricated through ball-milling method and low temperature melting *** influence of multi-walled carbon nanotubes (MWCNTs) on the mechanical strength and ductility of Sn-58Bi lead-free alloy was *** mechanical test results show that the bending strength of Sn-58Bi-0.03CNTs (mass fraction,%) composite is increased by 10.5% than that of the Sn-58Bi alloy,which can be attributed to the reduction of Sn-rich segregation and the grain *** toughness of Sn-58Bi-0.03CNTs composite is increased by 48.9% than that of the matrix *** is indicated that the influence of CNTs on the strength of Sn-58Bi-xCNTs composite is *** addition,the fracture mechanism of CNTs reinforced Sn Bi composite was *** corresponding fracture surface comparison between the Sn-58Bi-0.03CNTs composite and the monolithic Sn-58Bi alloy was made to identify the influence of CNTs on the fracture behavior and the reinforcing effect of CNTs.

关键词： carbon nanotubes metal-matrix composites mechanical properties fracture mechanism

A New Peptide with Membrane-permeable Function Derived from Human Circadian Proteins

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 629-636页

作者： Tao PENG, Ying-Hui LIU, chun-Lei YANG, Chao-Min WAN, Yao-Qi WANG, and Zheng-Rong WANG* Department of Biomedical Engineering, School of Basic Medicine and Forensic, Sichuan University, Chengdu 610041, China Department of Biomedical Engineering School of Basic Medicine and Forensic Sichuan UniversityChengdu 610041 China

Basic peptides such as human immunodeficiency virus type 1 (HIV-1) tat-(48-60) and Droso- phila Antennapedia-(43-58) have been reported to have a membrane permeability and a carrier function for intracellular protein delivery. Based on the fluorescence microscopic observations of the vascular endothelial cells (ECV-304) and the primary cultured neuroglial cells, we found that human Clock protein DNA-binding peptide [residue 35–47, hClock-(35-47)] had a translocation activity very similar to tat-(48-60). The cellular uptake of hClock-(35-47) increases with the increase of incubation time and concentration. The internalization effect at 4 °C was same as that at 37 °C. Internalization of hClock-(35-47) was saturable and could be inhibited by the excess of the other MPPs. Moreover, The uptake of these peptides were significantly inhibited in the presence of heparan sulfate. These results strongly suggested that the hClock-(35-47) shared a common or very similar internalization pathway with other MPPs. Furthermore, we injected rat through the common carotid artery with hClock-(35-47)-FITC peptide, and cryostat sections of the brain were prepared and observed using a fluorescence microscope. Result showed that the peptide had the ability to translocate through the blood-brain barrier. It is promising to provide a new safe carrier for the intracellular and encephalic treatment.

关键词： membrane-permeable peptide (MPP) cell membrane hClock DNA-binding peptide blood-brain barrier

Determinants for asthma control, quality of life and use of complementary and alternative medicine in asthmatic pediatric patients in four cities

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World Journal of Pediatrics 2018年第5期14卷 482-491页

作者： Kam Lun Hon Yan Min Bao Kate C.Chan Kin Wai Chau Rong-Shan Chen Kun tat Gary Cheok Wa Keung Chiu Li Deng chun-Hui He Kin Mui Ieong Jeng Sum C.Kung Ping Lam Shu Yan David Lam Qun Ui Lee So Lun Lee Ting Fan Leung Theresa N.H.Leung Lei Shi Ka Ka Siu Wei-Ping Tan Maggie Haitian Wang Tak Wai Wong Bao-Jing Wu Ada Y.F.Yip Yue-Jie Zheng Daniel K.Ng Department of Pediatrics The Chinese University of Hong Kong 6/F Prince of Wales HospitalRoom 84042ShatinN.THong Kong SARChina Department ofR espiratory Diseases Shenzhen Children’s HospitalShenzhen 518026China Hong Kong Society of P ediatric Respirology and Aller gy Hong K ong S ARChina Pediatric Respiratory andSleep Disorders Center Room 1315DArgyle Centre Phase 1688 Nathan RoadMongkokKowloonHong K ong S ARChina Department of R espiration Guangzhou Women and Children’s Medical CenterGuangzhou 510623China Department of P ediatrics Kiang Wu HospitalMacaoChina Department of P ediatrics and A dolescent Medicine United Christian Hospital130Hip Wo StreetKwun TongHong K ong S ARChina Department of P ediatrics Central Hospital Conde S.JanuarioMacaoChina Department of P ediatrics and A dolescent Medicine Caritas Medical Center111Wing Hong StreetSham Shui PoHong K ong S ARChina Department of P ediatrics and A dolescent Medicine Tuen Mun Hospital23 Tsing Chung Koon RoadTuen MunNewT erritoriesHongK ongS ARChina Department of P ediatrics and A dolescent Medicine Princess Margaret Hospital2-10 Princess Margaret Hospital RoadLai Chi KokKowloonHong K ong S ARChina Department of P ediatrics and A dolescent Medicine Queen Mary Hospital102Pokfulam RoadHong K ong S ARChina Department of P ediatrics and A dolescent Medicine The University of Hong KongHong K ong S ARChina Department of P ediatrics and A dolescent Medicine Pamela Youde Nethersole Eastern Hospital3Lok Man RoadChaiwanHong K ong S ARChina Department of P ediatrics The University of Hong Kong-Shenzhen Hospital1Haiyuan 1st RoadFutianShenzhenChina Department of P ediatrics and A dolescent Medicine Kwong Wah Hospital25 Waterloo RoadKLN Hong K ong S ARChina Department of P ediatrics Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China JC School of Public Health and Primary Care Faculty of MedicineThe Chinese University of Hong KongHong K ong S ARChina Department of P ediatrics and A dolescen

Background Asthma is a significant chronic health problem worldwide. Management aims at disease control by reducing functional impairment and exacerbations and improving quality of life (QoL). We report a multi-center study to survey asthma control and QoL in four cities in the Pearl River Delta. Methods The conjoint survey involved ten Hong Kong pediatric hospitals/units, two Shenzhen hospitals, two Macao hos-pitals, and two Guangzhou hospitals on asthma control (using Asthma Control Test) and QoL (Pediatric Allergic Disease Quality of Life Questionnaire, PADQLQ). Acceptability of a treatment is graded as very good/good/fair/poor. Results Good asthma control was only reported in 80% subjects in Hong Kong, but higher in sister cities (85–94%, P<0.001). Allergic rhinitis, 'incense burning', and 'smoker in family' were prevalent among the four cities. Logistic regres-sion showed better control of asthma was associated with better PADQLQ (B=?0.029, P < 0.001), better acceptability of bronchodilator (B=?1.488, P = 0.025), negatively with 'smoker in family' (B=?0.83, P = 0.015) and various PADQLQ domains. Conversely, worse PADQLQ was associated with allergic rhinitis severity (B=4.77, P <0.001), poor control of asthma (B=7.56, P <0.001), increased frequency of traditional Chinese medicine use (B=1.7, P < 0.05), increased fre-quency of bronchodilator usage (B=1.05, P < 0.05), 'smoker in family' (B=4.05, P < 0.05), and incense burning at home (B=3.9, P < 0.05). Conclusions There are some clinical and cultural differences among the four southern Chinese cities within the Guangdong province. This study identifies potentially modifiable environmental and treatment factors associated with poor asthma control and QoL for health-care interventions. Having a smoker in the family is independently associated with poor asthma control and QoL.

关键词： Asthma Complementary and alternative medicine Control Inhaled bronchodilator Inhaled corticosteroid Pediatric Allergic Disease Quality of Life Questionnaire Smoking Traditional Chinese medicine