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第十五届全国泌尿外科学术会议

作者： Zhang Kaiyan, Xing Jinchun, Chen Bin, Chen Shixin, Zhou Zhongquan, liu Rongfu, department of urology, XiaMen first hospital, affiliated to Fujian medical university, Xiamen , china 361003

Objective: To evaluate the outcome of urethral reconstruction using Barbagli procedure with buccal mucosa grafts in recurrence bulbar urethral strictures. Methods: 2 male patients, aged 42 years and 60 years, problem ...

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Characterization of Epididymal Protease Inhibitor Inhibiting the Activity of Prostate Specific Antigen

Characterization of Epididymal Protease Inhibitor Inhibiting...

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第十五届全国泌尿外科学术会议

作者： Zengjun Wang, JieZhang,Wei Zhang, Jiayin liu,Xinru Wang Laboratory of Reproductive Medicine , department of urology, the first affiliated hospital of Nanjing medical university 210029

Background:Analysis of a virtual structural model of Eppin constructed on Swiss-Model demonstrates that the C-terminal half of Eppin(amino acids 73-128) is structurally homologous to the Kunitz-type trypsin *** kunitz...

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Both Juxtacrine and Paracrine Signaling Indispensable in Spermatogonial Stem Cell Cultures

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Journal of Reproduction and Contraception 2010年第4期21卷 193-202页

作者： Tao XIONG Wei TANG Shi-xue liu Yun-feng HE Zi-wei TANG Jia-bing LI department of urology the First Affiliated Hospital Chongqing Medical University Chongqing 400016 China West china medical College Sichuan University Chengdu 610041 China The Third hospital of Mianyang Mianyang 621000 China

Objective To prove that juxtacrine and paracrine signaling are essential in the culture of spermatogonial stem cells （SSCs） with Sertoli cell feeder layer in vitro. Methods Mice aged 7 d were chosen to harvest testes. A two-step enzyme digestion method was applied in testis suspension. The SSCs and Sertoli cells were separated by adherence distinguishing methods and biologically identified by immunofluorescence and Oil Red 0 staining methods. Flow cytometry was used to analyze purity of SSCs. Three groups were constructed according to different culture conditions. SSC and Sertoli cell co-culture group, SSC conditional culture group and SSC routine culture group. The conditional medium was collected from supernate of culture Sertoli cell in vitro and double-concentrated with DMEM/F12 and fetal bovine serum in a proportion of 4.5 ： 4.5 ： 1. The routine medium was DMEM/F12 containing 10% fetal bovine serum. Adherence rates were measured by Trypan blue staining. Absorbance of SSCs of each group was measured by MTT assay and proliferation curves shown to demonstrate proliferative features of SSCs. Proliferative features and colony formation were observed by inverted microscope. With 24 h difference in adherence rates, proliferations were compared and *** The adherence rate of co-culture group was greater than that in the others（P〈0.05）, with insignificant difference in conditional culture group and routine group （P〉0. 05）. SSCs of co-culture group showed stable proliferation immediately following inoculation..4 stable colony formed within 7-10 d and maintained for 30 d. SSCs in conditional culture group and routine group decreased rapidly following transient proliferation. Conclusion The actions of SSCs in Sertoli cell cultures in vitro depended on both juxtacrine and paracrine signaling, Sertoli cell paraerine signaling was unable to promote SSC adherence and proliferation alone.

关键词： juxtacrine paracrine spermatogonial stem cells Sertoli cell in vitro

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Effect of IaParoscoPic rePair of vesicovaginal fistula

Effect of IaParoscoPic rePair of vesicovaginal fistula

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第十六届全国泌尿外科学术会议

作者： Wang dong-wen,Cao Xiao-ming,Guo Jin-xi,Xu Yun-fa, Liang Xue-zhi,Zhang Xu-hui,liu Chun,Cheng Wei. department of urology,The first hospital of shanxi medical university 030001

Objective:To exPlore the clinical value of laParoscoPic rePair of vesicovaginal ***:From Dec 2001 to Dec 2007,5 Patients of vesicovaginal fistula underwent laParoscoPic rePair *** the oPerating time,the

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Comparison between two operational techniques of vesicoureteral anastomosis (12 cases report)

Comparison between two operational techniques of vesicourete...

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第十五届全国泌尿外科学术会议

作者： liu Qingjun department of urology, Beijing Friendship hospital,Capital medical university, 100050

Operations f vesicoureteral anastomosis are now often used for the shortage of lower ureter. If the shortage of lower ureter is too long to be repaired directly, the operational techniques used are Boari-flap technique or psoas hitch technique. Both techniques have advantages and disadvantages. The data before have introduced the procedure clearly , and few research is focused on the changes

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Sperm retrieval rate and the predictive parameter of non-obstructive azoospermia:a meta-analysis from 1990 to 2006

Sperm retrieval rate and the predictive parameter of non-obs...

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第十三届全国泌尿外科学术会议暨第八届全球华人泌尿外科学术会议

作者： YANG Jun1, liu Ji-hong2, ZOU Xiao-feng1, ZHAN Ying2 1. department of urology, first affiliated hospital of Gannan medical university, Ganzhou, Jiangxi 341000, china 2. department of urology, Tongji hospital, Tongji medical College, Huazhong university of Science and Technology, Wuhan, Hubei 430030, china

Objective: To investigate sperm retrieval technique and sperm retrieval rate for non-obstructive azoospermic patients, and to evaluate the predictive value of different testicular pathological categories, clinical and...

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Expression change of the voltage-depended ca2+ channels in the asthenospermia

Expression change of the voltage-depended ca2+ channels in t...

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第十三届全国泌尿外科学术会议暨第八届全球华人泌尿外科学...

作者： Li Lu liu Jihong . department of urology Tongji hospital Tongji medical College Huazhong Science &Technology university Wuhan 430030 china

＜正＞Objective To study the relationship between expressions of the voltage-depended ca2+ channels （VDCCs） α1 mRNA in the oligospermia and the asthenospermia. Methods Based on the WHO criteria , the first to filte... 详细信息

关键词： Asthenospermia VDCCs CASA RT-PCR

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Gender disparity of living donor renal transplantation in china

Gender disparity of living donor renal transplantation in Ch...

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2012中国器官移植大会

作者： Guangxiang liu,Xiaogong Li,Tieshi liu,Xiaozhi Zhao,Hongqian Guo department of urology and Kidney Transplant Center,Nanjing Drum Tower hospital,The affiliated hospital of Nanjing university medical School,Nanjing,china

Gender disparity among living kidney donors is common world wide,which demonstrates different social and economic problems in different ***,few data are available ***,we retrospectively analyzed all 1 39 living donor renal transplants performed in our center between 2003 and *** annual number of living donor renal transplants increased from 6 to 26 cases per year during the observation *** them,69.2%of all kidney donors were female,

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Treatment with Vector-expressed Small Hairpin RNAs against Ki67 RNA-induced Cell Growth Inhibition and Apoptosis in Human Renal Carcinoma Cells

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 254-261页

作者： Jun-Nian ZHENG~(1,3*) Ya-Feng SUN~(2#) Dong-Sheng PEI~2 Jun-Jie liu~1 Teng-Xiang MA~3 Rui-Fa HAN~3 Wang LI~1 Dian-Bao ZHENG~1 Jia-Cun CHEN~1 Xiao-Qing SUN~1 1 Laboratory of urology,affiliated hospital of Xuzhou medical College,Xuzhou 221002,china 2 Research Center for Biochemistry and Molecular Biology,Xuzhou medical College,Xuzhou 221002,china 3 Institute of urology,medical university of Tianjin,Tianjin 300211,china Laboratoryofurology AffiliatedHospitalofXuzhouMedicalCollegeXuzhou221002China ResearchCenterforBiochemistryandMolecularBiology XuzhouMedicalCollegeXuzhou221002China Instituteofurology MedicalUniversityofTianjin

Short hairpin RNAs (shRNAs) transcribed *** polymerase Ⅲ promoters can triggersequence-selective gene silencing in mammalian *** virtue of their excellent function in knocking downexpression of cancer-associated genes,shRNAs could be used as new therapeutic agents for *** of Ki67 in renal cancer has been correlated to a more aggressive tumor phenotype,inhibitionof Ki67 protein expression by means of shRNAs seems to be a promising approach for the therapy of *** this study,we constructed an expression plasmid encoding shRNAs against the Ki67 gene,namedpSilencerKi67,and transfected it into human renal carcinoma *** pSilencerKi67 was shown to signifi-cantly knock down the expression of the Ki67 gene in human renal carcinoma cells,resulting in inhibitingproliferation and inducing apoptotic cell death that can be maintained for at least *** findings offer thepromise of using vector-based shRNAs against Ki67 in renal cancer gene therapy.

关键词： Ki67 gene shRNAs renal cell carcinoma proliferation apoptosis

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Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3#*)Ya-Feng SUN~(2#)Dong-Sheng PEI~2 Jun-Jie liu~1 Jia-Cun CHEN~1 Wang LI~1 Xiao-Qing SUN~1 Qi-Duo SHI~3 Rui-Fa HAN~3 Teng-Xiang MA~3 1 Laboratory of urology,affiliated hospital of Xuzhou medical College,Xuzhou 221002,china 2 Research Center for Biochemistry and Molecular Biology,Xuzhou medical College,Xuzhou 221002,china 3 Institute of urology,Tianjin medical university,Tianjin 300070,china Laboratoryofurology AffiliatedHospitalofXuzhouMedicalCollegeXuzhou221002China ResearchCenterforBiochemist'ryandMolecularBiology XuzhouMedicalCollegeXuzhou221002China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an RNA component(hTR)and a human telomerase reverse transcriptase component(hTERT).Smallinterfering RNA(siRNA),one kind of RNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of siRNA targeting at both hTRand hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells(HRCCs).Theproliferation and apoptosis of HRCCs were *** treatment of HRCCs using hTR and hTERTsiRNAs resulted in significant decrease of hTR mRNA,hTERT mRNA and hTERT *** siRNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy.

关键词： hTERT hTR telomerase siRNA renal cell carcinoma proliferation apoptosis

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