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Conformation Changes of Enkephalin in Coordination with Pb^(2+) Investigated by Gas Phase Hydrogen/Deuterium Exchange Mass Spectrometry Combined with Theoretical Calculations

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Chemical Research in Chinese Universities 2022年第2期38卷 572-578页

作者： HAN Jiaru WU Fangling YANG Shutong WU Xiaonan TANG Keqi LI Zhenhua ding chuan-Fan Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province Institute of Mass SpectrometrySchool of Material Science and Chemical EngineeringNingbo UniversityNingbo 315211P.R.China Department of Chemistry Fudan UniversityShanghai 200438P.R.China

In this study, the effects of lead ions(Pb^(2+)) on the conformations of leucine encephalin(LE) and methionine encephalin(ME) in gas phase were studied using hydrogen/deuterium exchange mass spectrometry(HDX-MS) and quantum chemistry theoretical calculations at the molecular level. The HDX-MS result revealed that the complexes with the monovalent compounds [LE+Pb–H]+ and [ME+Pb–H]+had a 1:1 stoichiometric ratio, and different HDX reactivates were observed in a follow of [ME+H]+>[LE+H]+>[LE+Pb–H]+> [ME+Pb–H]+. Combining the collision-induced dissociation energies of the complexes and their HDX results, it was found that the more stable the complex, the harder it was for HDX. In addition, the favo-rable conformations of the complexes were obtained by theoretical calculations, revealing that the similar coordination type with diffe-rent bond lengths was obtained. Then, the proton affinity(PA) values of the optimized complexes were calculated to interpret the HDX observations, indicating that the higher the PA values, the more difficult it was for HDX. Overall, the experiments and theoretical calculations revealed that Pb^(2+) could induce conformational changes of LE and ME, and generate ME into a more rigid conformation than LE. The results will prompt further fundamental investigations on the conformational properties of LE/ME in coordination with Pb^(2+).

关键词： Encephalin Pb^(2+) Gas phase-hydrogen/deuterium exchange(HDX) Theoretical calculation ESI-TOF-MS

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月季炭疽病内生拮抗细菌的筛选鉴定及抑菌作用

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南方农业学报 2024年第4期55卷 1046-1059页

作者：巫瑞袁美静康晓玲杜重阳孙瑞瑞丁传雨杜丽南阳师范学院生命科学与农业工程学院河南南阳473061 南阳师范学院河南省月季种质创新与栽培技术工程研究中心河南南阳473061 南阳师范学院分析测试中心河南南阳473061

【目的】发掘月季内生拮抗细菌资源,为月季炭疽病生物防治提供高效而稳定的拮抗菌株资源。【方法】以20个健康无病害蔷薇属植物的叶片为试验材料,采用组织匀浆法分离叶片内生细菌,采用平板对峙法筛选对月季炭疽病病原菌(Colletotrichumf... 详细信息

【目的】发掘月季内生拮抗细菌资源,为月季炭疽病生物防治提供高效而稳定的拮抗菌株资源。【方法】以20个健康无病害蔷薇属植物的叶片为试验材料,采用组织匀浆法分离叶片内生细菌,采用平板对峙法筛选对月季炭疽病病原菌(Colletotrichumfructicola)YJY-20具有拮抗作用的内生细菌菌株,结合菌落形态、生理生化和16S rRNA序列分析对拮抗菌进行种类鉴定。利用平板对扣法测定拮抗菌挥发性有机化合物的拮抗效果;利用牛津杯扩散法测定拮抗菌无菌发酵液对炭疽病病原菌及菌丝的影响;通过PCR扩增脂肽类抑菌物质合成基因;采用琼脂孔扩散法测定拮抗菌粗蛋白和脂肽类抑菌物质的抑菌活性;利用平板检测法测定拮抗菌产胞外酶活性;通过平板对峙法测定拮抗菌对12种植物病原真菌的抑制效果;采用离体叶片试验测定拮抗菌发酵液对月季炭疽病的防治效果。【结果】从蔷薇属植物叶片中分离筛选出2株对菌株YJY-20具有较强拮抗效果的内生细菌DMGSMG4和QZM1,经鉴定分别为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和贝莱斯芽孢杆菌(***)。菌株DMGSMG4和QZM1的挥发性有机化合物对菌株YJY-20的抑制率分别为11.72%和30.71%,同时能减少病原菌黑色素生成;其无菌发酵液对菌株YJY-20的抑制率分别为60.25%和61.24%,并能造成病原菌菌丝断裂扭曲。菌株DMGSMG4和QZM1粗蛋白对菌株YJY-20的抑制率分别为59.76%和50.63%,且热稳定性较好;其脂肽类抑菌物质对菌株YJY-20的抑制率分别为50.07%和45.03%。2株拮抗菌均能扩增出bmyB、fenD、srfAA和srfAB脂肽类抑菌物质合成相关基因。2株拮抗菌可产纤维素酶和蛋白酶,其中菌株DMSGMG4还可产有机酸。菌株DMGSMG4和QZM1对12种植物病原真菌的抑制率均大于50.00%,其对月季炭疽病离体叶片防治率分别为59.35%和20.58%。【结论】筛选获得的菌株DMGSMG4和QZM1对炭疽病病原菌具有较强的拮抗效果,具有作为月季炭疽病生防菌株的潜力。

关键词：月季炭疽病内生细菌拮抗菌生物防治

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Advancements in our understanding of circular and long non-coding RNAs in spinal cord injury

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Neural Regeneration Research 2022年第11期17卷 2399-2403页

作者： Yan Zhang Ho Jun Yun Yu Ji Eric Cosky Wen-Xiu Zhang Wei Han Yu-chuan ding Central Laboratory Beijing Luhe HospitalCapital Medical UniversityBeijingChina Department of Neurosurgery Wayne State University School of MedicineDetroitMIUSA Department of General Surgery Beijing Luhe HospitalCapital Medical UniversityBeijingChina

Spinal cord injury(SCI),either from trauma or degenerative changes,can res ult in severe disability and impaired quality of *** the cellular processes and molecular mechanisms that underlie SCI is imperative to identifying molecular targets for potential *** studies have shown that non-coding RNAs,including both long non-coding RNAs(lncRNAs)and circular RNAs(circRNAs),regulate various cellular processes in *** this review,we will describe the changes in lncRNA and circRNA expression that occur after SCI and how these changes may be related to SCI *** evidence for the roles of lncRNAs and circRNAs in neuronal cell death and glial cell activation will also be ***,the possibility that lncRNAs and circRNAs are novel modulato rs of SCI pathogenesis will be discussed.

关键词： circular RNAs function long non-coding RNAs neuronal cells neuronal glia pathogenesis regulation spinal cord injury

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基于雷达测量数据的弹箭扰动源最优估计

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中国惯性技术学报 2017年第4期25卷 544-549页

作者：丁传炳中国舰船研究设计中心上海201108

为精确计算舰载武器的飞行状态参数,以弹体纵向运动过程为研究对象,推导了包含误差干扰源在内的纵向扰动运动学方程,利用"系数冻结法"及拉普拉斯变换得到解析解,拟合出弹体被动段纵向运动的气动参数公式,采用三坐标雷达测量量作为系统... 详细信息

为精确计算舰载武器的飞行状态参数,以弹体纵向运动过程为研究对象,推导了包含误差干扰源在内的纵向扰动运动学方程,利用"系数冻结法"及拉普拉斯变换得到解析解,拟合出弹体被动段纵向运动的气动参数公式,采用三坐标雷达测量量作为系统量测方程,从而对气动参数误差干扰源进行最优估计。计算结果表明:该算法可以使俯仰操纵力矩系数导数误差的精度稳定在±0.007(°)^(-1)范围内,阻力系数误差的精度趋于±0.025之间;升力系数误差基本稳定在±0.12之间;俯仰力矩系数对攻角的导数偏差精度稳定在±0.011(°)^(-1)之间;俯仰阻尼力矩系数误差的精度收敛于±0.009(°)^(-1)之间,且算法收敛速度快,可为重构高精度的弹道参数提供支撑。

关键词：最优估计纵向运动扰动源雷达

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Electrosynthesis,Characterization and Optical Sensing Application of Amino Acid Functionalized Polyfluorene

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Chinese Journal of Polymer Science 2016年第2期34卷 229-241页

作者： Hui Zhang Ge Zhang 徐景坤文阳平 Wan-chuan ding Jie Zhang Shou-li Ming Shi-jie Zhen Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal UniversityNanchang 330013China Key Laboratory of Applied Chemistry Jiangxi Agricultural UniversityNanchang 330045China

An amino acid side chain functionalized polyfluorene derivative poly[N-（9-fluorenylmethoxycarbonyl）-glycine] （P9FG） was facilely electrosynthesized and characterized, and the structure, properties and optical sensing application of the obtained polymer were described and discussed. The electropolymerization occurred at C2 and C7 positions of fluorene units, and amino acid side chain groups were not cleaved from polyfluorene backbone in mixed electrolytes of boron trifluoride diethyl etherate and dichloromethane. Thermal analysis demonstrated good thermal stability of P9FG. Fluorescent spectra indicated that P9FG was a good blue light emitting material that could be employed as optical sensors. The soluble P9FG as a turn-off fluorescent sensor could realize the detection of Fe^3＋, Cu^2＋ and Cr2O7^2-, respectively. In addition, P9FG as a turn-off ultraviolet sensor could realize the detection of Cu^2＋ while as turn-on ultraviolet sensors could also realize the determination of Fe^3＋ and Cr2072-, respectively. All results indicate that P9FG is a promising candidate for optical sensing.

关键词： Conjugated polymers Polyfluorene derivatives Electropolymerization Optical sensors Metal ions.

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安全风险分级管控与“两票”的融合应用

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电力安全技术 2024年第7期26卷 6-9页

作者：梅增荣蒋三林朱志伟丁川王强华能澜沧江水电股份有限公司云南昆明650000 云南联合电力开发有限公司云南昆明650000

某公司在作业安全风险分级管控方面存在方法落后、人员不足、管理粗放、措施难以落地等问题。为健全安全风险分级管控和隐患排查治理双重预防工作机制,提升安全管控水平,在“两票”系统的基础上建立安全风险分级管控系统,实现“两票”... 详细信息

某公司在作业安全风险分级管控方面存在方法落后、人员不足、管理粗放、措施难以落地等问题。为健全安全风险分级管控和隐患排查治理双重预防工作机制,提升安全管控水平,在“两票”系统的基础上建立安全风险分级管控系统,实现“两票”系统与安全风险分级管控系统的联动,推进安全风险分级管控在具体生产作业流程中的应用。

关键词：安全风险分级管控两票风险数据库

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Death and do-not-resuscitate order in the emergency department:A single-center three-year retrospective study in the Chinese mainland

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World Journal of Emergency Medicine 2020年第4期11卷 231-237页

作者： chuan-qi ding Yu-ping Zhang Yu-wei Wang Min-fei Yang Sa Wang Nian-qi Cui Jing-fen Jin Department of Nursing the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhouChina Department of Emergency Medicine the Second Affi liated Hospital of Zhejiang University School of MedicineHangzhouChina

BACKGROUND:Consenting to do-not-resuscitate(DNR)orders is an important and complex medical decision-making process in the treatment of patients at the end-of-life in emergency departments(EDs).The DNR decision in EDs has not been extensively studied,especially in the Chinese ***:This retrospective chart study of all deceased patients in the ED of a university hospital was conducted from January 2017 to December *** patients with out-of-hospital cardiac arrest were ***:There were 214 patients’deaths in the ED in the three *** them,132 patients were included in this study,whereas 82 with out-of-hospital cardiac arrest were *** were 99(75.0%)patients’deaths after a DNR order medical decision,64(64.6%)patients signed the orders within 24 hours of the ED admission,68(68.7%)patients died within 24 hours after signing it,and 97(98.0%)patients had DNR signed by the family *** analysis showed that four independent factors infl uenced the family surrogates’decisions to sign the DNR orders:lack of referral(odds ratio[OR]0.157,95%confi dence interval[CI]0.047–0.529,P=0.003),ED length of stay(ED LOS)≥72 hours(OR 5.889,95%CI 1.290–26.885,P=0.022),acute myocardial infarction(AMI)(OR 0.017,95%CI 0.001–0.279,P=0.004),and tracheal intubation(OR 0.028,95%CI 0.007–0.120,P<0.001).CONCLUSIONS:In the Chinese mainland,the proportion of patients consenting for DNR order is lower than that of developed *** decision to sign DNR orders is mainly affected by referral,ED LOS,AMI,and trachea intubation.

关键词： Emergency service Do-not-resuscitate Death patients Retrospective analysis Emergency departments

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氧化锆纳米等离子涂层的结构与热震性能研究

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无机材料学报 2006年第1期21卷 250-256页

作者：梁波丁传贤中国科学院上海硅酸盐研究所上海200050

利用大气等离子喷涂技术,制备了氧化锆纳米结构和常规结构涂层,并对涂层的显微结构进行了探查．结果表明:纳米结构涂层是由直径为100nm左右的柱状晶粒组成的层状结构;涂层中存在大量的微裂纹和分布均匀的细小圆气孔．纳米结构的涂层具... 详细信息

利用大气等离子喷涂技术,制备了氧化锆纳米结构和常规结构涂层,并对涂层的显微结构进行了探查．结果表明:纳米结构涂层是由直径为100nm左右的柱状晶粒组成的层状结构;涂层中存在大量的微裂纹和分布均匀的细小圆气孔．纳米结构的涂层具有比常规涂层更好的抗热震性能,其热震行为与常规涂层不同．

关键词：等离子喷涂纳米结构氧化锆涂层热震性能

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Normobaric oxygen therapy attenuates hyperglycolysis in ischemic stroke

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Neural Regeneration Research 2021年第6期16卷 1017-1023页

作者： Zhe Cheng Feng-Wu Li Christopher R.Stone Kenneth Elkin Chang-Ya Peng Redina Bardhi Xiao-Kun Geng Yu-chuan ding Department of Neurology Luhe HospitalCapital Medical UniversityBeijingChina China-America Institute of Neuroscience Luhe HospitalCapital Medical UniversityBeijingChina Department of Neurosurgery Wayne State University School of MedicineDetroitMIUSA

Normobaric oxygen therapy has gained attention as a simple and convenient means of achieving neuroprotection against the pathogenic cascade initiated by acute ischemic *** mechanisms underlying the neuroprotective efficacy of normobaric oxygen therapy,however,have not been fully *** is hypothesized that cerebral hyperglycolysis is involved in the neuroprotection of normobaric oxygen therapy against ischemic *** this study,Sprague-Dawley rats were subjected to either 2-hour middle cerebral artery occlusion followed by 3-or 24-hour reperfusion or to a permanent middle cerebral artery occlusion *** 2 hours after the onset of ischemia,all rats received either 95%oxygen normobaric oxygen therapy for 3 hours or room *** with room air,normobaric oxygen therapy significantly reduced the infarct volume,neurological deficits,and reactive oxygen species and increased the production of adenosine triphosphate in ischemic *** changes were associated with reduced transcriptional and translational levels of the hyperglycolytic enzymes glucose transporter 1 and 3,phosphofructokinase 1,and lactate *** addition,normobaric oxygen therapy significantly reduced adenosine monophosphate-activated protein kinase mRNA expression and phosphorylated adenosine monophosphate-activated protein kinase protein *** findings suggest that normobaric oxygen therapy can reduce hyperglycolysis through modulating the adenosine monophosphate-activated protein kinase signaling pathway and alleviating oxidative injury,thereby exhibiting neuroprotective effects in ischemic *** study was approved by the Institutional Animal Investigation Committee of Capital Medical University(approval ***-2018-033)on August 13,2018.

关键词： neuroprotection oxidative stress oxygen pathways rat recovery repair stroke

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Measurement of Diffusion Coefficients of Air in Silicone Oil and in Hydraulic Oil

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Chinese Journal of Chemical Engineering 2011年第2期19卷 205-211页

作者：丁川范毓润 State Key Lab of Fluid Power Transmission and Control Zhejiang University Hangzhou 310027 China

A piston-cylinder apparatus was established to measure the solubility and diffusivity of air in dimethyl silicone oils and in hydraulic oils based on the PVT state equation of air and the solution of unsteady one-dimensional diffusion *** measured diffusivity-temperature relation can be well fitted by the Arrhenius equation for engineering *** correlation between the solute diffusivity D and solvent viscosity μ is *** terms of Eyring＇s activation theory,the activation in the air-silicone-oil diffusion process is quite different from that in the momentum transport of the silicone oil：the activation entropy of the former is positive while that of latter is ***,the activation enthalpies of the two processes are in the same order of magnitude,which leads to the observation that Dμ/T is roughly constant.

关键词： air silicone oil hydraulic oil diffusion coefficient viscosity

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