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Journal of Environmental Science and Engineering(A) 2013年第8期2卷 476-486页

作者： Nguyen Duy Can Vo Hong Tu chu thai hoanh Department of Rural Socio-Economics College of Rural Development Can Tho University Can Tho Vietnam Southeast Asia Regional Office IWMI (International Water Management Institute) Lao PDR Vientiane

An Giang province in the Mekong Delta is the most vulnerable province the impact of flooding and climate variability. Thousand of households are at risk due to severe annual floods. This study applied the LVI （livelihood vulnerability index） to estimate flood vulnerability of Phu Huu and Ta Danh villages in An Giang province. Data on socio-demographics, livelihoods, health, social networks, physical, financial and natural resources, natural disasters and climate variability were collected from a survey of 120 households in each village. From these data the LVI of each village was calculated. Results show that the overall LV1 of Phu Huu village, located in the early flooded zone, is higher than that of Ta Danh village, located in the late flooded zone. The analysis also indicated that this practical method can be applied for other purposes such as to monitor vulnerability, evaluate development programs or policy effectiveness by incorporating with scenario comparison.

关键词： An Giang province Mekong Delta livelihood vulnerability index flood and climate variability.

Comparative Chemical Research in Essential Oils from Six Apiaceae Species Growing in the Northern Region of Vietnam

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1677-1687页

作者： Nguyen Quang Hung Nguyen Thanh Tung Nguyen Phuong Hanh chu Thi Thu Ha Nguyen Thi Nhung Nguyen thai An Vu Xuan Giang Luong Van Hao Oleh Koshovyi Ain Raal Institute of Ecology and Biological Resources Vietnam Academy of Science and Technology(VAST)Cau GiayHa Noi100000Viet Nam Graduate University of Science and Technology Vietnam Academy of Science and Technology(VAST)Cau GiayHa Noi100000Viet Nam Department of Pharmacognosy Faculty of Pharmacognosy and Traditional MedicineHanoi University of PharmacyHoan KiemHa Noi100000Viet Nam Sa Pa Research Center of Medicinal Materials National Institute of Medicinal MaterialsDien Bien PhuSa PaLao Cai330000Viet Nam Institute of Pharmacy Faculty of MedicineUniversity of TartuTartu50411Estonia

Our study aimed to compare the essential oil(EO)concentration and composition of several Apiaceae species growing in the Northern region of *** yields of EOs from materials ranged from 0.03%(root EO of Angelica acutiloba and aerial parts EO of Heracleum bivittatum)-0.27%(leaf EO of Xyloselinum vietnamense).Gas chromatography-mass spectrometry(GC-MS)allowed the identiﬁcation of 74 components in the EOs of six Apiaceae species,making up 94.4%–100.0%of the *** EO from Angelica acutiloba,(Z)-ligustilide accounted for an extremely large proportion(94.9%).EO of Angelica pubescens was dominated by six character-istic components includingα-pinene(21.5%),β-phellandrene(18.1%),p-cymene(12.2%),3-methylnonane(8.7%),o-cymene(8.1%),and D-sylvestrene(6.2%).The EO from Cryptotaenia japonica was characterized by high amounts ofα-selinene(48.7%),β-selinene(23.7%),and trans-β-farnesene(5.4%).The EOs from leaves and stems of Xyloselinum vietnamense were characterized by high concentrations of sabinene(69.8%and 33.8%),4-terpineol(8.7%and 7.4%)andβ-pinene(4.0%and 6.5%)while EOs from aerial parts and root of Xylo-selinum leonidii comprise four characteristic monoterpenes includingα-pinene(28.2%and 52.8%),β-pinene(7.9%and 10.3%),β-phellandrene(7.6%and 15.3%),and sabinene(3.0%and 4.1%).Additionally,cryptone is also one of the major components in the EO of Xyloselinum leonidii(13.2%in the aerial parts oil and 2.8%in the root oil).In the EOs isolated from the aerial parts and root of Heracleum bivittatum,α-pinene(22.5%and 70.2%)andβ-pinene(43.2%and 20.0%)were the predominant *** appeared in the EO from aerial parts of Heracleum bivittatum with a relatively high concentration(13.5%)while bornyl acetate(5.1%)was also one of the main components in the EO from its aerial parts but was not detected in other Apiaceae species in the present *** databases help identify and control the quality of plant material studied from the family Apiaceae growing in Vietnam.

关键词： Apiaceae essential oil terpenoids gaschromatography-mass spectrometry

Molecular dynamics-driven exploration of peptides targeting SARS-CoV-2,with special attention on ACE2,S protein,M^(pro),and PL^(pro):A review

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BIOCELL 2023年第8期47卷 1727-1742页

作者： MOHAMAD ZULKEFLEE SABRI JOANNA BOJARSKA FAI-chu WONG TSUN-thai CHAI Green Chemistry and Sustainable Technology Cluster Bioengineering SectionUniversiti Kuala LumpurMalaysian Institute of Chemical and Bioengineering Technology(UniKL MICET)Alor Gajah78000Malaysia Institute of General and Ecological Chemistry Faculty of ChemistryLodz University of TechnologyLodz90-924Poland Department of Chemical Science Faculty of ScienceUniversiti Tunku Abdul RahmanKampar31900Malaysia Center for Agriculture and Food Research Universiti Tunku Abdul RahmanKampar31900Malaysia

Molecular dynamics(MD)simulation is a computational technique that analyzes the movement of a system of particles over a given *** can provide detailed information about the fluctuations and conformational changes of biomolecules at the atomic level over *** recent years,MD has been widely applied to the discovery of peptides and peptide-like molecules that may serve as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)*** review summarizes recent advances in such explorations,focusing on four protein targets:angiotensin-converting enzyme 2(ACE2),spike protein(S protein),main protease(M^(pro)),and papain-like protease(PL^(pro)).These four proteins are important druggable targets of SARS-CoV-2 because of their roles in viral entry,maturation,and infectivity of the virus.A review of the literature revealed that ACE2,S protein,and M^(pro) have received more attention in MD research than PL^(pro).Inhibitors of the four targets identified by MD simulations included peptides derived from food and other bioresources,peptides designed using the targets as templates,and peptide-like molecules retrieved from *** of the inhibitors have yet to be validated in experimental assays for ***,the role of MD simulation as an efficient tool in the early stages of anti-SARS-CoV-2 drug discovery agents has been demonstrated.

关键词： COVID-19 Anti-SARS-CoV-2 agent Receptor binding domain Computational screening Drug discovery

Antiviral activity of traditional Chinese medicinal plants Dryopteris crassirhizoma and Morus alba against dengue virus

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Journal of Integrative Agriculture 2020年第4期19卷 1085-1096页

作者： Maqsood MARYAM Kian Keong TE Fai chu WONG Tsun thai CHAI Gary K K LOW Seng Chiew GAN Hui Yee CHEE Department of Medical Microbiology and Parasitology Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdang 43400Malaysia Faculty of Natural Sciences SBK Women's University QuettaBalochistan 87300Pakistan Department of Chinese Medicine Faculty of Medicine and Health SciencesUniversiti Tunku Abdul RahmanSungai Long CampusKajang 43000Malaysia Department of Chemical Science Faculty of ScienceUniversiti Tunku Abdul Rahman(Kampar Campus)Kampar 31900Malaysia Department of Population Medicine Faculty of Medicine and Health SciencesUniversiti Tunku Abdul Rahman(Sungai Long Campus)Kajang 43000Malaysia Department of Pre-Clinical Sciences Faculty of Medicine and Health SciencesUniversiti Tunku Abdul Rahman(Sungai Long Campus)Kajang 43000Malaysia

Dengue virus(DENV) has emerged as a major virus that is spread by mosquitoes. Recently, it has spread to more than a hundred nations but continues to lack specific treatable medication. Many traditional Chinese medicinal(TCM) plants are in practice for dengue fever in dengue endemic regions. These traditional medicines persevere with treatments, which modern medicines lack. The study aims to substantiate the anti-dengue potential of some traditional herbs and make them available for further studies to facilitate TCM users. Twelve TCM plants aqueous extracts were evaluated, which are described as cool herbs used for the diseases with high fever. Lead plants were established through detailed in vitro foci forming unit reduction analysis(FFURA) against all four serotypes and validated through quantitative real-time RT-PCR(q RT-PCR). Four plants potentially inhibited the virus in primary phenotypic in vitro evaluation. Two lead plants Dryopteris crassirhizoma(DC) and Morus alba(MA) were identified with half minimal inhibitory concentration(IC50) 130 and 221 μg m L^-1, respectively, while the selectivity indices(SI) were 4.21 and 4.62, respectively. Lead plants equally inhibited all four serotypes of DENV. Time-of-addition analysis suggested that, DC was active at later stages of viral replication, whereas MA was active during the early stages and even showed some prophylactic activity. Liquid chromatography-mass spectrometry(HPLC/MS) analysis revealed presence of flavonoids. DC and MA are identified as potential anti-dengue plants, active against varied stages of dengue virus replication cycle. These results may serve as the base knowledge for further investigation on their combined treatments or integrative treatment with western medicines, which may improve the overall anti-dengue activity in future.

关键词： dengue virus traditional Chinese medicines TCM complementary medicines plant extracts anti-dengue

Dynamic of Polystyrene Microsphere Linking to DNA Molecule under Optical Tweezer

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Journal of Physical Science and Application 2014年第5期4卷 333-338页

作者： Lanh Van chu Dinh Trung thai Quang Quy Ho Vinh University Nghean Vietnam Institute of Applied Physics NEWTECHPRO VAST 8 Hoang Quoc Viet Hanoi Vietnam

Up to now there are many works investigated the stretched state of deoxyribonucleic axid （DNA） by optical trap. The main purpose of mentioned works is to find out the elastic force-extension characterisitic approximatelly based on the experimental parameters. In experiments, the optical force is seem as an apply force, but not an own elastic force of DNA molecules. To use sufficiently optical tweezer to manipulate and hold the DNA molecules keeping in stretched state, it is necessary to use the elastic as one force contributing to process trapping it, and consequently, the term describing elastic force must be present in the general Langevin equation （GLE） describing the dynamic of driven bead linking to DNA molecule. In this paper, the finite difference equation of GLE is derived for simulation the dynamic of bead linking to DNA molecule embedded in the fluid. The process manipulation of polystyrene bead to tweezer center is simulated and discussed. From results, the role of Brownian force, elastic force and optical force on process trapping driven bead is evaluated. Moreover, the trapping time, velocity of bead are discussed, too.

关键词： Optical tweezer Brownian motion Langevin equation DNA molecule stretch force.

Seagrass proteins: Sources of health-promoting agents awaiting exploration?

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Food Innovation and Advances 2023年第1期2卷 18-20页

作者： Tsun-thai Chai Jillian Lean Sim Ooi Fai-chu Wong Department of Chemical Science Faculty of ScienceUniversiti Tunku Abdul Rahman31900 KamparMalaysia Center for Agriculture and Food Research Universiti Tunku Abdul Rahman31900 KamparPerakMalaysia Department of Geography Faculty of Arts and Social SciencesUniversiti Malaya50603 Kuala LumpurMalaysia

Seagrasses are marine plants that form economically and ecologically important ecosystems in coastal seas around the *** seaweeds,another group of marine flora,seagrasses are flowering and seed-bearing ***,there are only 72 known species of seagrasses,distributed over six bioregions,spanning from the Temperate North Atlantic to the Temperate Southern Ocean[1].This contrasts with 295,383 species of terrestrial flowering plants[2]and 225,000 species of seaweeds known to science[3].

关键词： ecosystems coastal marine

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第1期20卷 59-70页

作者： Yixian QUAH Nor Ismaliza MOHD ISMAIL Jillian Lean Sim OOI Yang Amri AFFENDI Fazilah ABD MANAN Lai-Kuan TEH Fai-chu WONG Tsun-thai CHAI Department of Chemical Science Faculty of Science Universiti Tunku Abdul Rahman Department of Biological Science Faculty of Science Universiti Tunku Abdul Rahman Centre for Biodiversity Research Universiti Tunku Abdul Rahman Department of Geography Faculty of Arts and Social Sciences University of Malaya Institute of Biological Sciences Faculty of Science University of Malaya Institute of Ocean and Earth Sciences University of Malaya Department of Biosciences Faculty of Science Universiti Teknologi Malaysia Department of Biomedical Science Faculty of Science Universiti Tunku Abdul Rahman

Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay on the human cervical cancer(HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction(SPE), and reversed-phase high-performance liquid chromatography(RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ(428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration(EC50) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC50, AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.

关键词： Anticancer therapy Bioactive peptide Cytotoxicity HeLa cells Sarcophyton glaucum Soft coral

茴香种子蛋白质水解物和肽的抗氧化和蛋白质保护潜力（英文）

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现代食品科技 2019年第9期35卷 22-29,21页

作者：摩哈娜达斯·莎美拉蔡俊泰黄怀祖拉曼大学理学院马来西亚金宝31900 拉曼大学生物多样性研究中心马来西亚金宝31900

茴香种子是一种可食用的香料,经常用于增进各种烹饪的香气和味道,并且因其药用价值而在不同文化中被珍藏。之前的研究报告提及了与茴香种子相关的各种生物活性。然而,对于来自茴香种子的水解蛋白质知之甚少。在本文中,作者从茴香种子中... 详细信息

茴香种子是一种可食用的香料,经常用于增进各种烹饪的香气和味道,并且因其药用价值而在不同文化中被珍藏。之前的研究报告提及了与茴香种子相关的各种生物活性。然而,对于来自茴香种子的水解蛋白质知之甚少。在本文中,作者从茴香种子中分离出总蛋白质,然后使用四种不同的蛋白酶进行酶水解。本文中也报道了水解度,pH和温度曲线。在ABTS自由基清除测定的指引下,作者使用离心过滤,C18反相和强阳离子交换(SCX)来进一步分离碱性蛋白酶消化的蛋白质水解物。与粗水解物相比,纯化水解物的自由基清除潜力增强了4.5倍。通过肽测序进一步分析纯化的SCX级分,本文也鉴定出两个肽序列,其范围为6~8个氨基酸。紧接着,文中也报道了使用白蛋白变性抑制试验来测试茴香肽的蛋白质保护潜力,其中一种茴香肽(EDVDFR)更被测出与纯谷胱甘肽(GSH)相当的EC50值。总要言之,茴香种子衍生的蛋白质水解物和茴香肽有潜力被进一步开发成促进健康的补充剂或作为食品添加剂用于保存富含蛋白质的食物。

关键词：抗氧化肽酶水解茴香籽蛋白质保护 pH和热稳定性肠稳定性

Genetic diversity and inferred ancestry of Asian lotus(Nelumbo nucifera) germplasms in thailand and Vietnam

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Plant Diversity 2023年第1期45卷 69-79页

作者： Feng-Luan Liu Ya-Lan Dai Thi Nga Hoang Vichai Puripunyavanich Primlarp Wasuwat chukiatman Mi Qin Yan-Rong Fu Yu-chu Chen Dai-Ke Tian Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai201602PR China Plant Resources Center Vietnam Academy of Agricultural SciencesHanoi100803Vietnam thailand Institute of Nuclear Technology Bangkok10900Thailand Pang U Bon Waterlily Garden Muang NonthaburiNonthaburi11000Thailand Chinese Society of Landscape Architecture Beijing100835PR China Zhejiang Humanity Landscape Co. Ltd.HangzhouZhejiang310013PR China

Tropical lotus(Nelumbo) is an important and unique ecological type of lotus *** the genetic relationship and diversity of the tropical lotus is necessary for its sustainable conservation and *** 42 EST-SSR(expressed sequence tag-simple sequence repeats) and 30 SRAP(sequence-related amplified polymorphism) markers,we assessed the genetic diversity and inferred the ancestry of representative tropical lotus from thailand and *** total,164 and 41 polymorphic bands were detected in 69 accessions by 36 EST-SSR and seven SRAP makers,*** genetic diversity was revealed in thai lotus than in Vietnamese lotus.A Neighbor-Joining tree of five main clusters was constructed using combined EST-SSR and SRAP *** Ⅰ included 17 accessions of thai lotus;cluster Ⅱ contained three thai accessions and 11 accessions from southern Vietnam;and cluster Ⅲ was constituted by 13 accessions of seed *** with the results from the NeighborJoining tree,the genetic structure analysis showed that the genetic background of most thai and Vietnamese lotus was pure,as artificial breeding has been rare in both ***,these analyses indicate that thai and Vietnamese lotus germplasms belong to two different gene pools or *** lotus accessions are genetically related to geographical distribution patterns in thailand or *** findings showed that the origin or genetic relationships of some unidentified lotus sources can be evaluated by comparing morphological characteristics and the data of molecular *** addition,these findings provide reliable information for the targeted conservation of tropical lotus and parent selection in breeding novel cultivars of lotus.

关键词： Aquatic plant Asian lotus EST-SSR Nelumbo Southeast Asia Tropical lotus

Identification of Power “Knee Point”——A summary of the Forum on the Situation of Power Supply and Demand in 2006

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Electricity 2006年第2期17卷 9-12页

作者：无 chu Xue（译） Liu Wenying（审校） Staff Editor of China Power Enterprise Management 不详

With the mark of Chinese power installed capacity breaking through 500 GW, the power industry said goodbye to "electricity famine" and entered into the period of the 11th Five Year. Currently, the situations of power supply and demand in 2006 and the other four years of the 11th Five Year are undoubtedly of great concern both inside and outside the industry. Aiming at the discussions that the power supply would appear "knee point" and even "surplus," the Editorial Department of the China Power Enterprise Management held the Forum on the Situation of Power Supply and Demand in 2006, at which experts aired their own views on the matter of "knee point." The experts’ talks are shortened out and introduced as below.

关键词：能源拐点中国能源需求 "电荒" 分时用电电价 2006年