检索结果-南通市图书馆

Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2019年第2期17卷 119-128页

作者： Xiaotian Wu Shiqi Mao Yachen Ying christopher j.krueger Antony K. Chen Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China School of Life Sciences Peking University Beijing 100871 China Wallace H Coulter Department of Biomedical Engineering Georgia Institute of Technology Atlanta GA 30332 USA

Chromatin conformation,localization,and dynamics are crucial regulators of cellular behaviors. Although fluorescence in situ hybridization-based techniques have been widely utilized for investigating chromatin architectures in healthy and diseased states,the requirement for cell fix-ation precludes the comprehensive dynamic analysis necessary to fully understand chromatin activ-ities. This has spurred the development and application of a variety of imaging methodologies for visualizing single chromosomal loci in the native cellular context. In this review,we describe currently-available approaches for imaging single genomic loci in cells,with special focus on clus-tered regularly interspaced short palindromic repeats (CRISPR)-based imaging approaches. In addition,we discuss some of the challenges that limit the application of CRISPR-based genomic imaging approaches,and potential solutions to address these challenges. We anticipate that,with continued refinement of CRISPR-based imaging techniques,significant understanding can be gained to help decipher chromatin activities and their relevance to cellular physiology and pathogenesis.

关键词： CRISPR Cas9 dCas9 Genomic imaging sgRNA

Bi-FoRe:an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2021年第1期12卷 39-56页

作者： Bingzhou Han Yage Zhang Xuetong Bi Yang Zhou christopher jkrueger Xinli Hu Zuoyan Zhu Xiangjun Tong Bo Zhang Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education Peking University Genome Editing Research CenterCollege of Life SciencesPeking UniversityBeiing 100871China School of Biology and Biological Engineering South China University of TechnologyGuangzhou 510006China Department of Biomedical Engineering College of EngineeringPeking UniversityBeijing 100871China Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of Techno logy and EmoryAtlantaGA 33032USA Institute of Molecular Medicine Peking UniversityBejing 100871China

Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene ***,contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been *** we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles coupled with fluorescent allele-labeling through NHEj-mediated unidirectional targeted insertion in zebrafish facilitated by the CRISPR/Cas *** demonstrated the feasibility of this strategy at sox10 and isl1 loci,and successfully achieved Cre-induced conditional knockout of target gene function and simultaneous switch of the fluorescent reporter,allowing generation of genetic mosaics for lineage *** then improved the donor design enabling efficient one-step bidirectional knockin to generate paired positive and negative conditional alleles,both tagged with two different fluorescent *** introducing Cre recombinase,these alleles could be used to achieve both conditional knockout and conditional gene restoration in parallel;furthermore,differential fluorescent labeling of the positive and negative alleles enables simple,early and efficient realtime discrimination of individual live embryos bearing different genotypes prior to the emergence of morphologically visible *** named our improved donor as Bi-FoRe and demonstrated its feasibility at the sox10 ***,we eliminated the undesirable bacterial backbone in the donor using minicircle DNA *** system could easily be expanded for other applications or to other organisms,and coupling fluorescent labeling of gene expression and conditional manipulation of gene function will provide unique opportunities to fully reveal the power of emerging single-cell sequencing technologies.

关键词： CRISPR/Cas conditional knockout allele labeling conditional rescue minicircle DNA

Apples to oranges:environmentally derived,dynamic regulation of serotonin neuron subpopulations in adulthood?

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2596-2597页

作者： christopher j.O’Connell Matthew j.Robson Department of Genetics College of MedicineHarvard UniversityBostonMAUSA james L.Winkle College of Pharmacy Division of Pharmaceutical SciencesUniversity of CincinnatiCincinnatiOHUSA Neuroscience Graduate Program College of MedicineUniversity of CincinnatiCincinnatiOHUSA

Traumatic brain injury(TBI)is a public health problem with an undue economic burden that impacts nearly every age,ethnic,and gender group across the globe(Capizzi et al.,2020).TBIs are often sustained during a dynamic range of exposures to energetic environmental forces and as such outcomes are typically heterogeneous regarding severity and pathology(Capizzi et al.,2020).

关键词： sustained orange dynamic

Inhibition of retroviral Gag assembly by non-silencing miRNAs promotes autophagic viral degradation

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2018年第7期9卷 640-651页

作者： Na Qu Zhao Ma Mengrao Zhang Muaz N. Rushdi christopher j. krueger Antony K. Chen Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China Wallace H Coulter Department of Biomedical-Engineering Georgia Institute of Technology Atlanta GA 30332 USA

We recently reported an unconventional mechanism by which miRNAs inhibit HIV-1 viral production. This occurs when miRNAs bind nonspecifically to the viral structural protein Gag, interfering with viral RNA-medi- ated Gag assembly at the plasma membrane. Conse- quently, misassembled viral complexes are redirected into the endocytic pathway where they are delivered to lysosomes for degradation. In this study, we demon- strate that autophagy is a critical mediator of the viral degradation pathway and that this pathway is not HIV-1 specific. Misassembled viral complexes were found to colocalize extensively with LC3 and p62 in late endo- somesllysosomes, demonstrating a convergence of autophagy with functional degradative compartments. Knocking down autophagosome formation machineries reduced this convergence, while treatment with autop- hagy-inducer rapamycin enhanced the convergence. Furthermore, similar autophagy-dependent nonspecific miRNA inhibition of murine leukemia virus （MLV） assembly was shown. Overall, these results reveal autophagy as a crucial regulator of the retroviral degradation pathway in host cells initiated by non- specific miRNA-Gag interactions. These findings could have significant implications for understanding how cells may regulate retroviral complex assembly by miRNA expression and autophagy, and raise the possi- bility that similar regulations can occur in other biolog- ical contexts.

关键词： microRNA Gag protein autophagy

Studying turbulence structure near the wall in hydrodynamic flows:An approach based on the Schur decomposition of the velocity gradient tensor

在线全文

学校读者我要写书评

暂无评论

journal of Hydrodynamics 2022年第5期34卷 806-825页

作者： christopher j.Keylock School of Architecture Building and Civil EngineeringLoughborough UniversityLeicestershireUK

The Schur decomposition of the velocity gradient tensor(VGT)is an alternative to the classical Cauchy-Stokes decomposition into rotation rate and strain rate ***,there have been several strands of work that have employed this decomposition to examine the physics of turbulence dynamics,including approaches that combine the Schur and Cauchy-Stokes *** are briefly reviewed before the latter approach is set *** partitions the rotation rate and strain rate tensors into normal/local and non-normal/non-local *** then study the relation between the VGT dynamics and ejection-sweep events in a channel flow *** show that the sweeps in particular exhibit novel behaviour compared with either the other quadrants,or the flow in general,with a much-reduced contribution to the dynamics from the non-normal terms above the viscous *** particular,the reduction in the production term that is the interaction between the non-normality and the normal straining reduces in the log-layer as a consequence of an absence of alignment between the non-normal vorticity and the strain rate *** have been early forays into using the Schur transform approach for subgrid-scale modelling in large-eddy simulation(LES)and this would appear to be an exciting way forward.

关键词： Turbulence channel flow velocity gradient tensor Schur decomposition

Development process for a higher capacity Propsetter~ system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International journal of Mining Science and Technology 2018年第1期28卷 121-126页

作者： christopher j.Brown Product Development-Strata Worldwide LLC

As longwall tailgate support technologies continue to be developed and improved, strata worldwide has worked to design and manufacture a revised Propsetter to provide additional support capacity and enable applications at greater mine *** support is targeted for tailgate applications, however, it can also be utilized for roof support in various types of mining *** paper describes the design changes for enhancing the support capacity of the Propsetter to more closely align its performance with other support technologies currently being employed in longwall *** changes to each design aspect of the Propsetter was key to successfully improving the roof support's load capacity while maintaining controlled *** the load capacity would allow a mine to improve support efficiency with a smaller, less intrusive support compared to support alternatives and reduce the cost per foot of supported *** target support capacity was 711–890 k N at 50 mm of deformation to more closely match the performance of competing tailgate supports such as the 610 mm-diameter CAN~.To achieve this goal, changes were made to all design aspects of the Propsetter: diameter, wedge cut depth and angle, pod size, and confinement *** results from the NIOSH Mine Roof Simulator verified that these design changes were successful in increasing the capacity of the Propsetter to the 711–890 k N range at fifty mms of deformation.

关键词： Propsetter Can Longwall mining Tailgate roof support Controlled deformation

Plasma-catalysis: Is it just a question of scale?

在线全文

学校读者我要写书评

暂无评论

Frontiers of Chemical Science and Engineering 2019年第2期13卷 264-273页

作者： j.christopher Whitehead School of Chemistry The University of ManchesterManchester M13 9PLUK

The issues of describing and understanding the changes in performance that result when a catalyst is placed into plasma are discussed. The different chemical and physical interactions that result and how their combination might produce beneficial results for the plasma-catalytic processing of different gas streams are outlined with particular emphasis being placed on the different range of spatial and temporal scales that must be considered both in experiment and modelling. The focus is on non-thermal plasma where the lack of thermal equilibrium creates a range of temperature scales that must be considered. This contributes in part to a wide range of inhomogeneity in different properties such as species concentrations and electric fields that must be determined experimentally by in situ methods and be incorporated into modelling. It is concluded that plasmacatalysis is best regarded as conventional catalysis perturbed by the presence of a discharge, which modifies its operating conditions, properties and outcomes often in a very localised way. The sometimes used description "plasma-activated catalysis" is an apt one.

关键词： plasma catalysis plasma-activated catalysis non-thermal plasma CO2 conversion

Endocasts-the direct evidence and recent advances in the study of human brain evolution

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2007年第9期17卷 993-1002页

作者： christopher j.Norton 86011-4099 AZ Beijing 100044 China Flagstaff Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Laboratory of Quaternary Paleontology Northern Arizona University USA

Brain evolution is one of the most important aspects of human evolution,usually studied through *** of fossil hominid endocasts allows inferences on functional anatomy,physiology,and *** this paper,we describe the general features of endocast studies and review some of the major topics in *** are:absolute and relative brain size evolution;brain shape variation;brain asymmetry and lateralization;middle meningeal vessels and venous sinuses;application of computed tomography and virtual imaging;the history of Chinese brain endocast *** particular,this review emphasizes endocast studies on Chinese hominin fossils.

关键词： endocast human fossil brain evolution cranial capacity human evolution

Structural subgrid-scale modeling for large-eddy simulation: A review

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Mechanica Sinica 2016年第4期32卷 567-578页

作者： Hao Lu christopher j.Rutland School of Energy and Power Engineering Huazhong University of Science and Technology Engine Research Center Mechanical EngineeringUniversity of Wisconsin-Madison

Accurately modeling nonlinear interactions in turbulence is one of the key challenges for large-eddy simulation（LES） of turbulence. In this article, we review recent studies on structural subgrid scale modeling, focusing on evaluating how well these models predict the effects of small scales. The article discusses a priori and a posteriori test results. Other nonlinear models are briefly discussed, and future prospects are noted.

关键词： turbulence turbulent viscosity priori dissipation posteriori scalar A review scales Reynolds