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Polycomb response element-binding sites in the MDR of CLL: Potential tumor suppressor regulation

Advances in Bioscience and Biotechnology 2013年第1期4卷 129-135页

作者： christine e. Cutucache Javeed Iqbal Philip J. Bierman Robert Gregory Bociek Dennis D. Weisenburger Shantaram S. Joshi Department of Biology University of Nebraska at Omaha Omaha USA Department of Genetics Cell Biology and Anatomy University of Nebraska Medical Center Omaha USA Department of Internal Medicine Oncology/Hematology University of Nebraska Medical Center Omaha USA Department of Pathology and Microbiology University of Nebraska Medical Center Omaha USA

Chronic lymphocytic leukemia [CLL] is the most common adult leukemia and is heterogeneous in clinical presentation. CLL cases present with various chromosomal aberrations, including 11q23, 14q32, 17p, and trisomy 12, with the most common abnormality being deletion of 13q14 [1]. Although monoallelic deletion of 13q14 is common, there is a subset of patients who have complete nullisomy at 13q14, a locus that has been hypothesized to contribute to CLL pa thogenesis [2] due to loss of tumor suppressors [DLeU and miR-15a/16-1]. We hypothesized that deletion of both copies of 13q14 would lead to uncontrollable proliferation of CLL cells and a poor prognosis. We examined our 13q14 nullisomy for survival, treatment-free survival, lymphocyte doubling time, and the presence of lymphadenopathy. Furthermore, we compared the gene expression profiles between patients with 13q14 monosomy, nullisomy, or normal karyotype. Our results suggest that patients with 13q nullisomy have a higher incidence of bulky lymphadenopathy [16.6% compared to 10% of monosomy patients], a higher frequency of lymphocyte doubling time [27.7% compared to 7.4% of monosomy patients], and a higher rate of needing treatment [50% compared to 18.5% of monosomy patients]. We observed deletion of DLeU1 and HTR2A, consistent with a gene dosage effect, and observed PRe-binding sites on DLeU1. Patients with homozygous deletion of 13q14 had a worse prognosis compared to heterozygotes. Lastly, the DLeU1 locus is a possible “second hit” loss for CLL progression.

关键词： Chronic Lymphocytic Leukemia Gene expression 13q14 Nullisomy DLeU Tolerogenic

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Two Highly Variable Vpr84and Vpr85Residues within the HIV-1-Vpr C-Terminal Protein Transduction Domain Control Transductionnal Activity and Define a Clade Specific Polymorphism

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World Journal of AIDS 2014年第2期4卷 148-155页

作者： Jean-Herve Colle Thiery Rose christine Rouzioux Alphonse Garcia eA 3620 Universite Paris Descartes AP-HP Laboratoire de Virologie Hopital Necker Paris France Laboratoire e3 Phosphatases Unite Signalisation Moleculaire et Activation Cellulaire Institut Pasteur Paris France Unite d’Immunogenetique Cellulaire Institut Pasteur Paris France Unite de Biologie des Populations Lymphocytaires Institut Pasteur Paris France

The virally encoded HIV-1 viral protein R (VPR) is a multifunctional factor that is required for induced HIV-1 pathogenesis. VPR is also a cell-penetrating protein found in biological fluids from HIV-1 infected individuals. In this regard, we previously published that the C-terminal VPR77-92 sequence from HIV-1 89.6, but not from pNL4.3 strain, is a new pro-apoptotic and protein transduction domain (PTD). Here we report on a sequence analysis of VPR77-92 domain using the Los Alamos HIV-1 sequence database. The analysis showed that the two residues of the domain VPR84 and VPR85 are highly variable and differently biased in HIV-1 clade B and HIV-1 clade C. Furthermore, when Jurkat lymphoblastoid cells or PBMC were incubated with chemically synthesized peptides containing distinct VPR77-92 C-terminal sequences from clades B or C, we found that a clade-dependent polymorphism in VPR84 and VPR85 residues controlled the transducing activity of the C-terminal HIV-1 VPR77-92 domain. Together our data indicate that clade-dependent polymorphism in the VPR84 and VPR85?residues defines the transducing properties mediated by the C-terminal domain of HIV-1 VPR. Identification of this VPR polymorphism suggests new approaches to understand the HIV-1 biology and/or pathogenesis.

关键词： VPR HIV-1 Subtypes Polymorphism Transduction

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Chitosan—The Application of a Natural Polymer against Iron Hydroxide Deposition

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American Journal of Analytical Chemistry 2016年第8期7卷 623-632页

作者： Simona Schwarz christine Steinbach Dana Schwarz Mandy Mende Regine Boldt Leibniz-Institut für Polymerforschung Dresden e.V. Dresden Germany Department of Organic Chemistry Faculty of Science Charles University in Prague Prague Czech Republic

As a consequence of mining, heavy metal ions can be exposed to the environment hence contaminate ground water and surface water amongst others. The natural polymer chitosan was proved to be an excellent adsorber material for the effective removal of iron and sulfate ions in batch as well as in column experiments. The adsorption behavior of iron ions, as well as sulfate ions was investigated by utilizing chitosan flakes as a natural adsorbent. The removal was studied using adsorbance measurements, SeM and SeM-eDX. The adsorption capacity of chitosan was determined at different times. The received adsorption capacities for iron ions were very promising with a maximum adsorption capacity of 85 mg/g and a rate of separation of 100%. The maximum adsorption capacity obtained for sulfate ions was 188.8 mg/g and a rate of 80%.

关键词： Chitosan Adsorption of Iron- and Sulfate Ions Iron hydroxide Deposition

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Treatment of Peripheral Neuropathy: Combination Therapy Using LeD Light, extracorporeal Shockwave Therapy, Platelet Rich Plasma, and an Oral Dietary Supplement

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International Journal of Clinical Medicine 2023年第5期14卷 250-259页

作者： Alex Spinoso Robert Settineri christine McLaren Garth L. Nicolson elite Medical Corona CA USA Sierra Productions Research Irvine California USA Department of Medicine University of California Irvine Irvine CA USA Institute for Molecular Medicine Huntington Beach CA USA

Objectives: Peripheral neuropathy (PN) is a significant contributor to disability in the elderly. It is also one of the most prevalent complications of type 2 diabetes, prediabetes and metabolic syndrome. PN is commonly associated with pain, numbness, tingling, burning, and cramping in the feet and legs. Current treatment options are limited to controlling pain, seizures and use of antidepressant medications. These treatments have undesirable side effects and don’t stop PN progression. Here we utilized a combination of individual-specific modalities to improve local circulation and relieve PN symptoms. Methods: We conducted an open-label, multicenter pilot trial with 34 subjects (19 males and 15 females ranging from 40 - 85 years of age). All of the participants were diagnosed with peripheral neuropathy and had bilateral symptoms in their feet, and many reported the same symptoms (pain, numbness, tingling, burning, and cramping) in their lower legs. The duration of symptoms ranged from four months to over six years. On Day 0, subjects were given a 90-day supply of the oral supplement with dosing instructions and a LeD light therapy device. They also received three platelet-rich plasma (PRP) injections in their lower extremities. Subjects also received an extracorporeal shockwave therapy (eSWT) treatment for each foot and subsequently twice per week for the first six weeks, then once weekly for the duration of the study. Subjects filled out the Brief Pain Index (BPI) at weekly intervals. On Day 90, subjects completed the Patient Global Impression of Change (PGIC) survey. Results: There were significant responses to pain, as evidenced by BPI scores at weeks 8, 9, 10 and 11 (p = 0.02, 0.01, 0.02, and 0.003, respectively). Analysis of the final day PGIC survey showed a favorable outcome for 73% of participants (p = 0.003), with the majority reporting Very Much Improved. Conclusions: By utilizing a multi-modality treatment protocol that includes PRP, LeD light therapy, e

关键词： Peripheral Neuropathy LeD Light Therapy Device extracorporeal Shockwave Therapy PRP Injections Oral Supplement Multi-Modality Treatment Pain

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Size-Dependent Joule Heating of Gold Nanoparticles Using Capacitively Coupled Radiofrequency Fields

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Nano Research 2009年第5期2卷 400-405页

作者： christine H.Moran Sean M.Wainerdi Tonya K.Cherukuri Carter Kittrell Benjamin J.Wiley Nolan W.Nicholas Steven A.Curley John S.Kanzius Paul Cherukuri Department of Bioengineering Rice University6100 Main StreetHoustonTX 7005USA Department of Surgical Oncology MD Anderson Cancer Center1515 Holcombe BlvdBox 107HoustonTX 77030USA Department of Bioengineering Texas A&M UniversityCollege StationTX 77843USA Department of Chemistry and Richard e.Smalley Institute for Nanoscale Science and Technology Rice University6100 Main StreetHoustonTX 77005USA Department of Chemistry and Chemical Biology Harvard University12 Oxford StreetCambridgeMA 02138USA Department of Physics and Richard e.Smalley Institute for Nanoscale Science and Technology Rice University6100 Main StreetHoustonTX 77005USA Department of Mechanical engineering and Material Science Rice University6100 Main StreetHoustonTX 77005USA Department of experimental Therapeutics MD Anderson Cancer Center1515 Holcombe BlvdBox 107HoustonTX77030USA

Capacitively coupled shortwave radiofrequency fields(13.56 MHz)resistively heat low concentrations(~1 ppm)of gold nanoparticles with a thermal power dissipation of~380 kW/g of *** diameter gold nanoparticles(eat at nearly twice the rate of larger diameter gold nanoparticles(≥50 nm),which is attributed to the higher resistivity of smaller gold nanostructures.A Joule heating model has been developed to explain this phenomenon and provides critical insights into the rational design and engineering of nanoscale materials for noninvasive thermal therapy of cancer.

关键词： Resistivity radiofrequency gold nanoparticles cancer thermal

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基础法语

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2015年

作者：王尚民 [法] christine GONNeT-BOULLY

本书共分为语音、语法的基础知识、语法和课文三部分，主要内容包括：语音常识、前元音、清辅音、浊辅音、法语字母表、几项读音规则等。

ISBN: (纸本)9787507829518

本书共分为语音、语法的基础知识、语法和课文三部分，主要内容包括：语音常识、前元音、清辅音、浊辅音、法语字母表、几项读音规则等。

关键词：法语自学参考资料

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Methodology Article: Can Ruminal Reducing Power Assessed in Batch Cultures be Comparable to in VivoMeasurements?

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Journal of Analytical Sciences, Methods and Instrumentation 2014年第3期4卷 80-86页

作者： christine Julien Jean-Philippe Marden Annabelle Troegeler-Meynadier Corine Bayourthe INRA UMR1388 Genetic Physiology and Breeding System Castanet-Tolosan France Lesaffre Feed Additives 282 Avenue de la Marne Marcq-en-Baroeul France

In ruminant field of digestive research, the appeal to methods of less invasive studies and reproducing the in vivo conditions is essential. The objective of the present study was to determine whether the conditions created with the proposed in vitro batch culture was an accurate reproduction of the physico-chemical and fermentative ruminal conditions observed in vivo. Two experiments were conducted to compare ruminal reducing power measured in vitro, i.e. in batch cultures or, in vivo i.e. in live animals: dairy cows at maintenance (experiment 1) and lactating dairy cows (experiment 2). In experiment 1, at the beginning of incubation period, in vitro redox potential (eh), pH and Clark’s exponent (rH) values were significantly higher than in vivo (+42 mV, +0.25 and +1.9, respectively) whereas volatile fatty acids (VFA) contents were 2.6 fold lower on average. At the end of incubation, eh, rH values and VFA contents were similar between both methods whereas pH still remained different. In experiment 2, at the beginning and at the end of incubation period, in vitro eh, pH and rH values differed significantly than in vivo. As a result, the in vitro method did not provide a tool to evaluate accurately the level of the reducing status of ruminal milieu compared with in vivo measurement. Nonetheless, it provided strong reducing conditions after 8 h of incubation with levels of rH relatively closed to those observed in vivo. In vitro batch culture could be a good alternative to in vivo trials for a screening approach from an ethic and economic point of view in ruminant field of research.

关键词： Batch Cultures Clark’s exponent Redox Potential

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A critical window for cooperation and competition among developing retinotectal synapses

A critical window for cooperation and competition among deve...

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CCAST“量子纠缠态与量子信息”研讨会

作者： Li I. Zhang Huizhong W. Tao christine e. Hoitt William A. Harris Mu-ming Poo

＜正＞ In the developing frog visual system, topographic refinement of the retinotectal projection dependa on electrical activity. In vivo whole-cell recording from developlng Xenopus tectal neurons shows that convergent retinotectal synapses undergo activity-dependent cooperation and competition following correlated pre-and postsynaptic spiking within a narrow time window. Synaptic Inputo activated repetitively within 20 ms before spiking of the tectal neuron become potentiated, whereas subthreshold Inputs activated within 20 ma after spiking become depressed. Thus both the initial synaptic strength and the temporal order of activation are critical for heterosynaptic Interactions among convergent synaptic Inputs during activity-dependent refinement of developing neural networks.

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Antimicrobial Activity of the Methanolic extract and Compounds from the Stem Bark of Drypetes tessmanniana

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Chinese Journal of Integrative Medicine 2010年第4期16卷 337-343页

作者： Victor Kuete Marlise D.J.Dongfack Armelle T.Mbaveng Marie-christine Lallemand Hanh T.Van-Dufat Jean-Duplex Wansi elisabeth Seguin Francois Tillequin Jean Wandji Department of Biochemistry Faculty of ScienceUniversity of Dschang.P.O.Box 67 DschangCameroon Department of Organic Chemistry Faculty of ScienceUniversity of Yaounde I.P.O.Box 812 YaoundeCameroon Laboratoire de Pharmacognosie de I'Universite Paris Descartes UMR/CNRS N° 8638Faculte des Sciences Biologiques et Pharmaceutiques4-Avenue de I'Observatoire75006 ParisFrance Department of Biochemistry Faculty of ScienceUniversity of Yaounde IP.O.Box 812 YaoundeCameroon Department of Chemistry University of DoualaFaculty of ScienceP.O.Box 24157 DoualaCameroon Laboratoire de Pharmacognosie UFR de Medecine Pharmacie Rouen22-Bid Gambetta76183 Rouen Cedex 1France

Objective： To evaluate the antimicrobial activity of the methanol extract from the stem bark of Drypetes tessmanniana, fractions （DTB1-5） as well as compounds [friedelin （2）, 3,7-dioxofriedelane （3）, 3,15-dioxofriedelane （4）, 313 -O-（e）-3,5-dihydroxycinnamoyl-11 -oxo-olean-12-ene （6）, and 313,6 α -dihydroxylup- 20（29）-ene （7）. Methods： Agar disc diffusion was used to determine the sensitivity of the above samples, whilst the microdilution method was used for the determination of the minimal inhibitory concentration （MIC） and the minimal microbicidal concentrations （MMC）. Results： The diffusion test showed that the crude extract was able to prevent the growth of all tested organisms. All other samples showed selective activity. The inhibitory effect of the fraction DTB2 was noted on 63.7%, that of DTB1 and DBT3 on 54.6%, whilst DTB4 and DTB5 were active on 9.1% of the 11 tested organisms. The tested compounds prevented the growth of 81.8% of the tested microbial species for compounds 3 and 4, 36.7% for compound 6, and 18.2% for compound 7. The results of the MIC determinations indicated perceptible values for DTB and compound 4 on 81.8% of the tested organisms. For other samples, MICs were detected on 0-63.7%. The lowest MIC value （78.12 μg/mL） for the crude extract and fractions （DTB2） was observed on M. audouinii. The corresponding value for isolated compounds （156.25 μg/ mL） was noted with compounds 3 on S. faecalis and 4 on M. audouinii. The results of the MMC determination suggested that the microbicidal effect of most of the tested samples on the studied microorganisms could be expected. Conclusion： The methanol extract from the stem bark of Drypetes. tessmanniana （euphorbiaceae） as well as some of the isolated compounds might be potential sources of new antimicrobial drues.

关键词： Drypetes tessmanniana euphorbiaceae compounds antimicrobial activity

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Mechano-responsive hydrogel for direct stem cell manufacturing to therapy

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Bioactive Materials 2023年第6期24卷 387-400页

作者： Yufeng Shou Ling Liu Qimin Liu Zhicheng Le Khang Leng Lee Hua Li Xianlei Li Dion Zhanyun Koh Yuwen Wang Tong Ming Liu Zheng Yang Chwee Teck Lim christine Cheung Andy Tay Department of Biomedical engineering National University of Singapore117583Singapore Institute for Health Innovation&Technology National University of Singapore117599Singapore School of Civil engineering and Architecture Wuhan University of Technology430070WuhanChina NUS Tissue engineering Program National University of Singapore117510Singapore Lee Kong Chian School of Medicine Nanyang Technological University636921Singapore School of Mechanical and Aerospace engineering Nanyang Technological University639798Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Mechanobiology Institute National University of Singapore117411Singapore

Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory *** cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in *** overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to *** our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and *** addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-eRK *** our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.

关键词： Mesenchymal stem cell Dynamic mechanical stimulation Magnetic hydrogel Stem cell manufacturing Cell therapy

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