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Molecular Epidemiology and Risk Factors of Carbapenem-Resistant Klebsiella Pneumoniae Bloodstream Infections in wuhan,China

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Current Medical Science 2022年第1期42卷 68-76页

作者： Chan LIU Lan LIU ming-ming JIN Yang-bo HU Xuan CAI Lu wan Hai-yue ZHANG Rui-yun LI Xiao-jun wu Department of Respiratory and Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhan 430060China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430060China Department of Clinical Laboratory Renmin Hospital of Wuhan UniversityWuhan 430060China

Objective:The clinical characteristics and microbiological data of patients with *** bloodstream infections(BSI)from January 2018 to December 2020 were retrospectively analyzed to study the molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae(CRKP).We also aimed to identify the risk factors for the development of CRKP ***:This retrospective study was conducted at Renmin Hospital of wuhan University from January 2018 to December *** date of non-duplicate *** isolates isolated from blood samples was identified using the microbiology laboratory *** data from patients diagnosed with *** BSI were collected and analyzed.

关键词： Carbapenem-resistant Klebsiella pneumoniae bloodstream infection

Pollen Germination Activates the Apical Membrane-Located RAC/ROP GTPase Switch

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Molecular Plant 2013年第4期6卷 1358-1361页

作者： christine Y.-h. Chen Wen-Guang Zheng Alice Y. Cheung Hen-ming wu Department of Biochemistry and Molecular Biology Molecular and Cell Biology Program University of Massachusetts Amherst MA 01003 USA Millipore Corporation 28820 Single Oak Drive Temecula CA 92590 USA Department of Genetics Development and Cell Biology Iowa State University Ames IA 50011 USA Plant Biology Graduate Program

Dear Editor, Pollen germination and tube growth underlie reproduc- tive success in flowering plants. Pollen germination, whether occurring on the stigmatic surface of the pistil or under defined medium conditions in vitro, involves producing a polarized outgrowth that extends at the tip, producing a pollen tube. RAC/ROPs, the RHO GTPases of plants, are molecu- lar switches that pivotally control the polarized pollen tube growth process. However, how RAC/ROP activities respond to conditions during germination and tube growth to signal the needed cellular events has not been examined. We show here that tobacco pollen germination rapidly induces RAC/ ROP activation, potentially affecting myriad downstream cellular processes that support pollen tube growth.

关键词： GERMINATION GUANOSINE triphosphatase POLLEN PLANTS -- Phosphorylation PLANT membranes

Numerical simulations of FPSO with sloshing tanks in a random freak waves

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Journal of Hydrodynamics 2022年第3期34卷 491-498页

作者： Yuan Zhuang Guo wang De-cheng wan Jian-wei wu Computational Marine Hydrodynamics Lab(CMHL) School of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China Marine Design and Research Institute of China Shanghai200011China wuhan Second Ship Design and Research Institute Wuhan430205China

This paper presents a potential-viscous coupled method to simulate a floating production storage and offloading(FPSO)with two liquefied natural gas(LNG)sloshing tanks in a random freak *** potential theory uses high-order-spectral(HOS)*** random wave is generated by HOS for 2000 s,and a freak wave is observed around 1830 *** FPSO LNG model was firstly verified in regular waves using HOS-computational fluid dynamics(CFD)coupled method and validated the HOS-CFD coupled method can solve the ship motion coupled with sloshing tanks *** FPSO with LNG tanks are then simulated in the freak wave,showed the coupling effects when the freak wave *** the existence of the sloshing tanks,the amplitude of the roll motion decreases and the period of roll motion *** breaking phenomenon can be observed when the wave crest of the freak wave encounters with the ship.

关键词： Ship motion with sloshing tank high-order-spectral-computational fluid dynamics(HOS-CFD)method naoe-FOAM-SJTU solver freak waves

Scalable and controllable synthesis of 2D high-proportion 1T-phase MoS_(2)

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Nano Research 2020年第11期13卷 2933-2938页

作者： Xiang Gao Liukang Xiong Jiabin wu Jun wan Liang Huang wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan430074China State Key Laboratory of Materials Processing and Die&Mold Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan430074China

Two-dimensional molybdenum disulfide(2D MoS_(2))is considered as a promising candidate for many applications due to its unique structure and ***,the controllable synthesis of large-scale and high-quality 2D 1T-phase MoS_(2)is still a ***,we present the scalable and controllable synthesis of 2D MoS2 from 2H to 1T@2H phase by using K_(2)SO_(4)salt as a simultaneous high-temperature sulfur source and *** as-synthesized 1T@2H-2D MoS_(2)exhibits a high yield and can be easily assembled into freestanding electrode with high specific capacitance of 434 F/g at a scan rate of 1 mV/s in LiClO_(4)ethylene carbonate/dimethyl carbonate(EC/DMC).Moreover,various single-crystal 2D transition metal sulfides(WS_(2),PbS,MnS and Ni_(9)S_(8))and 2D S-doped carbon can be synthesized using this *** believe that this study may provide a new sight for scalable and controllable synthesis of other 2D materials beyond 2D MoS_(2).

关键词： two-dimensional material MoS_(2) 1T phase scalable synthesis controllable synthesis

Clinic-pathological features of metabolic associated fatty liver disease with hepatitis B virus infection

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World Journal of Gastroenterology 2021年第4期27卷 336-344页

作者： ming-Fang wang Bo wan Yin-Lian wu Jiao-Feng Huang Yue-Yong Zhu You-Bing Li Department of Liver Research Center The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005Fujian ProvinceChina Institute of Neurology University College LondonLondon WC1N 3BGUnited Kingdom

BACKGROUND Metabolic associated fatty liver disease(MAFLD)is a novel concept proposed in *** To compare the characteristics of MAFLD and MAFLD with hepatitis B virus(HBV)*** Patients with histopathologically proven MAFLD from a single medical center were *** were divided into MAFLD group(without HBV infection)and HBV-MAFLD group(with HBV infection).Propensity score matching was utilized to balance the baseline characteristics between two *** A total of 417 cases with MAFLD were included,359(86.1%)of whom were infected with *** were significantly more males in the HBV-MAFLD group than in the MAFLD group(P0.05).The rank sum test results showed that the degree of liver steatosis in the MAFLD group was more severe than that in the HBV-MAFLD group,while the degree of inflammation and fibrosis in the liver was less severe(P<0.05).In multivariate analysis,HBV infection was associated with significantly lower grade of hepatic steatosis[odds ratio(OR)=0.088,95%confidence interval(CI):0.027-0.291]but higher inflammation level(OR=4.059,95%CI:1.403-11.742)and fibrosis level(OR=3.016,95%CI:1.087-8.370)after adjusting for age,gender,and other metabolic *** HBV infection is associated with similar metabolic risks,lower steatosis grade,higher inflammation,and fibrosis grade in MAFLD patients.

关键词： Fatty liver disease Metabolic associated fatty liver disease Hepatitis B virus Biopsy Clinic-pathological features

Optimized Mask-RCNN model for particle chain segmentation based on improved online ferrograph sensor

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Friction 2024年第6期12卷 1194-1213页

作者： Shuo wanG Miao wan Tonghai wu Zichen BAI Kunpeng wanG Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing System Xi’an Jiaotong UniversityXi’an 710049China

Ferrograph-based wear debris analysis(WDA)provides significant information for wear fault analysis of mechanical *** decades of offline application,this conventional technology is being driven by the online ferrograph sensor for real-time wear state ***,online ferrography has been greatly limited by the low imaging quality and segmentation accuracy of particle chains when analyzing degraded lubricant oils in practical *** address this issue,an integrated optimization method is developed that focuses on two aspects:the structural re-design of the online ferrograph sensor and the intelligent segmentation of particle *** enhancing the imaging quality of wear particles,the magnetic pole of the online ferrograph sensor is optimized to enable the imaging system directly observe wear particles without penetrating ***,a light source simulation model is established based on the light intensity distribution theory,and the LED installation parameters are determined for particle illumination uniformity in the online ferrograph *** this basis,a Mask-RCNN-based segmentation model of particle chains is constructed by specifically establishing the region of interest(ROI)generation layer and the ROI align layer for the irregular particle *** these measures,a new online ferrograph sensor is designed to enhance the image acquisition and information extraction of wear *** verification,the developed sensor is tested to collect particle images from different degraded oils,and the images are further handled with the Mask-RCNN-based model for particle feature *** results reveal that the optimized online ferrography can capture clear particle images even in highly-degraded lubricant oils,and the illumination uniformity reaches 90%in its imaging *** importantly,the statistical accuracy of wear particles has been improved from 67.2%to 94.1%.

关键词： wear particle analysis online ferrography particle image acquisition particle chain segmentation

In_(2)O_(3-)x(OH)y/Bi_(2)MoO_(6) S-scheme heterojunction for enhanced photocatalytic performance

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Journal of Materials Science & Technology 2020年第21期56卷 151-161页

作者： Zhongfu Li Zhaohui wu Rongan He Long wan Shiying Zhang College of Materials Science and Engineering Hunan UniversityChangsha410082China Hunan Key Laboratory of Applied Environmental Photocatalysis Changsha UniversityChangsha410005China

Surface heterojunction engineering has been extensively studied to promote efficient charge separation in semiconductor *** an effective heterojunction system to optimize the separation and transport of photo-induced charges is an appealing strategy to enhance the photocatalytic *** this work,In_(2)O_(3)-x(OH)y in situ decorated Bi_(2)MoO_(6) two-dimensional step-scheme heterojunctions were synthesized through a controlled dehydroxylation process of indium-based *** charge transfer mechanism of this step-scheme heterojunctions was confirmed by the characterization of electron structures,reactive species,photoelectric properties and DFT theoretical *** band bending and the internal electric field caused by the charge transfer upon hybridization can effectively promote the separation of charges and present the optimal redox *** addition,surface residual hydroxyl groups can regulate the surface energy state and optimize the interfacial charge transfer kinetics of the prepared step-scheme ***,the step-scheme heterojunction exhibits superior performance in photocatalytic reduction of hexavalent chromium and degradation of organic pollutants under visible light *** work provides an innovative perspective to construct photocatalyst with superior activity.

关键词： Step-scheme Heterojunction Bi_(2)MoO_(6) Photocatalyst

Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm,Spodoptera exigua(Hübner)

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Science China(Life Sciences) 2011年第2期54卷 152-158页

作者： GUO JianYing wu Gang wan FangHao State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China College of Plant Sciences and Technology Huazhong Agricultural University Hubei 430070 China

Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet armyworms,Spodoptera exigua(Hübner),fed on transgenic Bacillus thuringiensis(Bt) and non-Bt cotton for different time *** composition and specific enzyme activities differed significantly between the *** fed Bt ***-Bt *** 1,6 and 24 h,free fatty acid and glucose levels were significantly lower in *** fed on Bt cotton than those fed on non-Bt *** fed on Bt cotton had significantly higher trypsin and total superoxide dismutase(T-SOD) activities and significantly lower lipase,carboxylesterase and acetylcholinesterase activities than non-Bt fed worms for all feeding time *** were also observed among feeding times within each cotton variety *** lower free fatty acid and total amino acid were observed in *** fed on Bt cotton for 24 h than in those fed for 1 *** lower activities of lipase and trypsin were detected in *** fed on Bt cotton for 24 h than those for 1 and 4 ***,carboxylesterase and acetylcholinesterase activities in *** fed on Bt cotton for 24 h were significantly higher than those for 1,4 and 6 *** interaction between cotton variety and feeding time significantly affected the activities of lipase,trypsin,acetylcholinesterase and T-SOD enzymes in *** the temporal allocation of protection and detoxification enzyme activities in the body of *** in response to *** can provide a meaningful evaluation on the metabolic tolerance of herbivorous insects under the continuous selection pressure of a toxic protein.

关键词： Bacillus thuringiensis Spodoptera exigua detoxification enzyme carboxylesterase acetylcholinesterase

Tracking a maneuvering target in clutter with out-of-sequence measurements for airborne radar

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Journal of Systems Engineering and Electronics 2015年第4期26卷 746-753页

作者： Weihua wu Jing Jiang Yang wan Air Force Early Warning Academy Unit 94627 of the PLA

There are many proposed optimal or suboptimal al- gorithms to update out-of-sequence measurement（s）（OoSM（s）） for linear-Gaussian systems, but few algorithms are dedicated to track a maneuvering target in clutter by using OoSMs. In order to address the nonlinear OoSMs obtained by the airborne radar located on a moving platform from a maneuvering target in clut- ter, an interacting multiple model probabilistic data association （IMMPDA） algorithm with the OoSM is developed. To be practical, the algorithm is based on the Earth-centered Earth-fixed （ECEF） coordinate system where it considers the effect of the platform＇s attitude and the curvature of the Earth. The proposed method is validated through the Monte Carlo test compared with the perfor- mance of the standard IMMPDA algorithm ignoring the OoSM, and the conclusions show that using the OoSM can improve the track- ing performance, and the shorter the lag step is, the greater degree the performance is improved, but when the lag step is large, the performance is not improved any more by using the OoSM, which can provide some references for engineering application.

关键词： out-of-sequence measurement(s) (OoSM(s)) Earth-centered Earth-fixed (ECEF) interacting multiple model (IMM),probabilistic data association (PDA) attitude.