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Genomic resources for the brown planthopper, Nilaparvata lugens： Transcriptome pyrosequencing and microarray design

Insect Science 2012年第1期19卷 1-12页

作者： chris bass Martin Bay Hebsgaard Joseph Hughes Centre for Sustainable Pest and Disease Management Rothamsted Research Harpenden University of Edinburgh Institute of Evolutionary Biology Kings Buildings Edinburgh Institute of Biodiversity Animal Health and Comparative Medicine College of Medical Veterinary and Life Sciences University of Glasgow Glasgow United Kingdom

The brown planthopper, Nilaparvata lugens is a pest of cultivated rice through- out Asia and is controlled using insecticides and/or resistant rice varieties. This species has developed resistance to many classes of insecticide and biotypes have developed that are vir- ulent against formerly resistant rice cultivars. Insects use a suite of detoxification enzymes, including cytochrome P450s, glutathione S-transferases and carboxyl/cholinesterases to defend themselves against plant secondary metabolites and pesticides. Pyrosequencing on the Roche 454-FLX platform was used to produce a substantial expressed sequence tag （EST） dataset to complement the existing Sanger sequenced ESTs in GenBank. A total of 78 959 reads were combined with the 37 392 publically available Sanger ESTs; these assembled into 8 911 contigs and 10 620 singletons. Analysis of the distribution of tenta- tive unique genes （TUGs） with the gene ontology for biological processes and molecular functions suggests that the 454 and Sanger EST assembly is broadly representative of the N. lugens transcriptome. The brown planthopper transcriptome was found to contain 31 TUGs encoding P450s, nine encoding glutathione S-transferases and 26 encoding car- boxyl/cholinesterases and many of these are putatively involved in the detoxification of xenobiotics. The Agilent eArray platform was used to construct an oligonucleotide mi- croarray populated with probes for ~ 19 000 unigene sequences, including all those known to encode detoxification enzymes. The genomic resources developed in this study will be useful to the community studying this crop pest and will help elucidate the molecular mechanism underlying insecticide resistance and planthopper adaptation to resistant rice cultivars.

关键词： brown planthopper detoxification enzymes insecticide resistance Nilaparvata lugens transcriptome sequencing

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Molecular and functional analysis of chitin synthase genes in Chilo suppressalis(Lepidoptera:Crambidae)

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Insect Science 2023年第3期30卷 661-676页

作者： Bin Zeng Fu-Rong Chen Hao Sun Yan Liu Shun-Fan Wu chris bass Cong-Fen Gao State&Local Joint Engineering Research Center of Green Pesticide Invention and Application/College of Plant Protection Nanjing Agricultural UniversityNanjingChina College of Life and Environmental Sciences BiosciencesUniversity of ExeterPenrynUK

The rice stem borer,Chilo suppressalis,has developed a high level of resistance to many of the compounds currently used for *** is therefore an urgent need to develop novel control methods for *** chitin synthases(CHS)have attracted interest as a potential target for insect pest ***,to date,CHS have not been characterized in *** CHS genes(CsCHSI and CsCHS2)were identified and cloned from *** transcript variants were identified for CsCHS1,CsCHSla and *** expression profiling showed that both transcripts of CsCHS1 are most highly expressed on the last day of each larval instar stage and show the highest expression levels in the *** contrast,CsCHS2 is predominantly expressed during the larval feeding stages and shows the highest expression levels in the *** of CsCHSI by RNA interference significantly inhibited the molting and pupation of ***,and knockdown of CsCHS2 significantly affected growth during the larval stage,but had no significant effect on the ***,knockout of CsCHS1 by CRISPR/Cas9 genome editing severely lowered the hatching rate,larval survivorship,pupation rate,and eclosion rate,but only larval survivorship at the GO generation was lowered after the knockout of *** results demonstrate that CsCHS1 and CsCHS2 play vital roles in the growth and development of ***,and so have potential as insecticidal targets for the control of this highly damaging pest.

关键词： Chilo suppressalis chitin synthase CRISPR/Cas9 pest control RNA interference

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Are type IV pili involved in <i>Vibrio anguillarum</i>virulence towards sea bass (<i>Dicentrarchus labrax</i>L.) larvae?

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Agricultural Sciences 2013年第6期4卷 30-38页

作者： Ingeborg Frans Pieter Busschaert Kristof Dierckens chris W. Michiels Kris A. Willems Bart Lievens Peter Bossier Hans Rediers Centre for Food and Microbial Technology M2S KU Leuven Leuven Belgium Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM) Thomas More Mechelen Campus De Nayer Department of Microbial and Molecular Systems (M2S) KU Leuven Association Sint-Katelijne-Waver Belgium Scientia Terrae Research Institute Sint-Katelijne-Waver Belgium Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM) Thomas More Mechelen Campus De Nayer Department of Microbial and Molecular Systems (M2S) KU Leuven Association Sint-Katelijne-Waver Belgium Scientia Terrae Research Institute Sint-Katelijne-Waver Belgium Centre for Food and Microbial Technology M2S KU Leuven Leuven Belgium Laboratory of Aquaculture & Artemia Reference Center Department of Animal Production Ghent University Ghent Belgium Laboratory of Aquaculture & Artemia Reference Center Department of Animal Production Ghent University Ghent Belgium

Vibrio anguillarum, an important bacterial fish pathogen, expresses a variety of virulence factors contributing to its ability to cause vibriosis in fish. Many virulence factors of this pathogen remain however unknown. For example, a type IV pilus system was previously reported to be potentially involved in the virulence of this bacterium but no experimental evidence was reported yet. In this study, complete genome sequencing of V. anguillarum strain VIB15, shown to be highly virulent towards sea bass (Dicen- trarchus labrax L.) larvae, revealed the presence of a PilA pilin. A V. anguillarum VIB15 pilA mutant was constructed and the pathogenicity of this mutant was assessed in a gnotobiotic sea bass system developed for virulence screening. Our results suggest that the V. anguillarum pilA gene is not crucial for virulence towards sea bass larvae. Possibly, another type IV pilus system identified in V. anguillarum, showing homology to the mannose-sensitive hemagglutinin pilin of Vibrio cholerae, may complement the pilA mutation. Alternatively, the type IV pilus system has a role in infection of juvenile or adult fish, rather than in the larval phase. As such, further research is required to unravel the potential role of type IV pili in V. anguillarum virulence.

关键词： Virulence Vibrio anguillarum Fish Pathogen Type IV Pili

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