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Modulative effect of Physalis alkekengi on both gut bacterial and fungal micro-ecosystem

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Chinese Herbal Medicines 2023年第4期15卷 564-573页

作者： Yanan Yang Xiaohui Zhao Yong Xie chongming wu Pharmacology and Toxicology Research Center Institute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China School of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjin 301617China

Objective: Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi(PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic ***, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largely unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between ***: Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, ***: Oral administration of PAL notably enriched anti-infammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-infammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus,Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups(groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum,Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus ***: Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechan

关键词： gut bacteria gut fungi Physalis alkekengi L. traditional Chinese medicine micro-ecosystem

Overexpressing HRS1 Confers Hypersensitivity to Low Phosphate-Elicited Inhibition of Primary Root Growth in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2009年第4期51卷 382-392页

作者： Hong Liu Huixia Yang chongming wu Juanjuan Feng Xin Liu Huanju Qin Daowen Wang State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

Phosphate （Pi） deficiency causes dramatic root system architecture （RSA） changes in higher plants. Here we report that overexpression of HRS1 leads to enhanced sensitivity to low Pi-elicited inhibition of primary root growth in Arabidopsis thaliana seedlings. Bioinformatic investigations uncovered that HRS1 and its six homologs encode putative G2-like transcription factors in Arabidopsis. Analysis of promoter：：GUS reporter lines revealed that HRS1 transcripts were present mainly in the root hair region and root hair cells under Pi-sufficient conditions. Pi deprivation increased HRS1 expression level and expanded its expression domain. Although HRS1 knockout mutant did not differ from wild type （WT） control irrespective of Pi status, its overexpression lines were significantly more susceptible to low Pi-elicited primary root shortening. In both WT and HRS1 overexpression seedlings, low Pi-induced primary root shortening was accompanied by enhanced root hair cell differentiation, but this enhancement occurred to a greater extent in the latter genotype. Collectively, our data suggest that HRS1 may be involved in the modulation of primary root and root hair growth in Pi-deprived Arabidopsis seedlings, and provide useful clues for further research into the function of HRS1 and its homologs and the mechanisms behind RSA changes under Pi-deficient conditions.

关键词： Arabidopsis thaliana G2-like transcription factor HRS1 phosphate deficiency root.

Cordycepin promotes browning of white adipose tissue through an AMP-activated protein kinase(AMPK)-dependent pathway

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Acta Pharmaceutica Sinica B 2019年第1期9卷 135-143页

作者： Guihong Qi Yue Zhou Xiaopo Zhang Jiaqi Yu Xin Li Xiaoxue Cao chongming wu Peng Guo Pharmacology and Toxicology Research Center Institute of Medicinal Plant Development Chinese Academy of Medical Sciences &Peking Union Medical College School of Pharmaceutical Science Hainan Medical University

Obesity is a worldwide epidemic. Promoting browning of white adipose tissue(WAT)contributes to increased energy expenditure and hence counteracts obesity. Here we show that cordycepin(Cpn), a natural derivative of adenosine, increases energy expenditure, inhibits weight gain, improves metabolic profile and glucose tolerance, decreases WAT mass and adipocyte size, and enhances cold tolerance in normal and high-fat diet-fed mice. Cpn markedly increases the surface temperature around the inguinal WAT and turns the inguinal fat browner. Further investigations show that Cpn induces the development of brown-like adipocytes in inguinal and, to a less degree, epididymal WAT depots. Cpn also increases the expression of uncoupling protein 1(UCP1) and other thermogenic genes in WAT and3T3-L1 differentiated adipocytes, in which AMP-activated protein kinase(AMPK) plays an important role. Our results provide novel insights into the function of Cpn in regulating energy balance, and suggest a potential utility of Cpn in the treatment of obesity.

关键词： Cordycepin Browning of white adi pose tissue(WAT) Thermogenesis AMP-activated protein kinase(AMPK) Obesity

Potential efficacy and mechanism of eight mild-natured and bitterflavored TCMs based on gut microbiota:A review

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Chinese Herbal Medicines 2024年第1期16卷 42-55页

作者： Wenquan Su Yanan Yang Xiaohui Zhao Jiale Cheng Yuan Li Shengxian wu chongming wu Dongzhimen Hospital Beijing University of Chinese MedicineBeijing 100700China School of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjin 301617China Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin 301617China

The mild-natured and bitter-flavored traditional Chinese medicines(MB-TCMs)are an important class of TCMs that have been widely used in clinical practice and recognized as safe long-term treatments for chronic ***,as an important class of TCMs,the panorama of pharmacological effects and the mechanisms of MB-TCMs have not been systemically *** studies have shown that gut microbiota can mediate the therapeutic activity of TCMs and help to elucidate the core principles of TCM medicinal *** this systematic review,we found that MB-TCMs commonly participated in the modulation of metabolic syndrome,intestinal inflammation,nervous system disease and cardiovascular system disease in association with promoting the growth of beneficial bacteria Bacteroides,Akkermansia,Lactobacillus,Bifidobacterium,Roseburia as well as inhibiting the proliferation of harmful bacteria Helicobacter,Enterococcus,Desulfovibrio and *** alterations,correspondingly,enhance the generation of protective metabolites,mainly including short-chain fatty acids(SCFAs),bile acid(BAs),5-hydroxytryptamine(5-HT),indole and gamma-aminobutyric acid(GABA),and inhibit the generation of harmful metabolites,such as proinflammatory factors trimethylamine oxide(TAMO)and lipopolysaccharide(LPS),to further exert multiplicative effects for the maintenance of human health through several different signaling ***,this present review has attempted to comprehensively summarize the relationship between MB-TCMs and gut microbiota by establishing the TCMs-gut microbiota-metabolite-signaling pathway-diseases axis,which may provide new insight into the study of TCM medicinal theories and their clinical applications.

关键词： bile acid gamma-aminobutyric acid 5-hydroxytryptamine mild-natured and bitter-flavored traditional Chinese medicines property theory short-chain fatty acids traditional Chinese medicine trimethylamine oxide

Syringaresinol-4-O-β-D-glucoside alters lipid and glucose metabolism in HepG2 cells and C2C12 myotubes

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Acta Pharmaceutica Sinica B 2017年第4期7卷 453-460页

作者： Shuai Wang chongming wu Xin Li Yue Zhou Quanyang Zhang Fuchao Ma Jianhe Wei Xiaopo Zhang Peng Guo Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Research Centre on Life Sciences and Environment Sciences Harbin University of Commerce School of Pharmaceutical Science Hainan Medical University

Syringaresinol-4-O-β-D-glucoside(SSG), a furofuran-type lignan, was found to modulate lipid and glucose metabolism through an activity screen of lipid accumulation and glucose consumption, and was therefore considered as a promising candidate for the prevention and treatment of metabolic disorder,especially in lipid and glucose metabolic homeostasis. In this study, the effects of SSG on lipogenesis and glucose consumption in Hep G2 cells and C2C12 myotubes were further investigated. Treatment with SSG significantly inhibited lipid accumulation by oil red O staining and reduced the intracellular contents of total lipid, cholesterol and triglyceride in Hep G2 cells. No effect was observed on cell viability in the MTT assay at concentrations of 0.1–10 μmol/L. SSG also increased glucose consumption by Hep G2 cells and glucose uptake by C2C12 myotubes. Furthermore, real-time quantitative PCR revealed that the beneficial effects were associated with the down-regulation of sterol regulatory element-binding proteins-1c,-2(SREBP-1c,-2), fatty acid synthase(FAS), acetyl CoA carboxylase(ACC) and hydroxyl methylglutaryl CoA reductase(HMGR), and up-regulation of peroxisome proliferator-activated receptors alpha and gamma(PPARα and PPARγ). SSG also significantly elevated transcription activity of PPARγtested by luciferase assay. These results suggest that SSG is an effective regulator of lipogenesis and glucose consumption and might be a candidate for further research in the prevention and treatment of lipid and glucose metabolic diseases.

关键词： Syringaresinol-4-O-β-d -glucoside Lipid accumulation Glucose consumption Insulin resistance HepG2 C2C12 Oil red O

Equisetin is an anti-obesity candidate through targeting 11β-HSD1

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2358-2373页

作者： Zhenlu Xu Dongyun Liu Dong Liu Xue Ren Haibo Liu Guihong Qi Yue Zhou chongming wu Kui Zhu Zhongmei Zou Jing Yuan Wenhan Lin Peng Guo Pharmacology and Toxicology Research Center Institute of Medicinal Plant DevelopmentChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100193China Capital Institute of Pediatrics Beijing 100020China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China National Center for Veterinary Drug Safety Evaluation College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China

Obesity is increasingly prevalent globally, searching for therapeutic agents acting on adipose tissue is of great importance. Equisetin(EQST), a meroterpenoid isolated from a marine sponge-derived fungus, has been reported to display antibacterial and antiviral activities. Here, we revealed that EQST displayed anti-obesity effects acting on adipose tissue through inhibiting adipogenesis in vitro and attenuating HFD-induced obesity in mice, doing so without affecting food intake, blood pressure or heart *** demonstrated that EQST inhibited the enzyme activity of 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), a therapeutic target of obesity in adipose tissue. Anti-obesity properties of EQST were all offset by applying excessive 11β-HSD1’s substrates and 11β-HSD1 inhibition through knockdown in vitro or 11β-HSD1 knockout in vivo. In the 11β-HSD1 bypass model constructed by adding excess11β-HSD1 products, EQST’s anti-obesity effects disappeared. Furthermore, EQST directly bond to11β-HSD1 protein and presented remarkable better intensity on 11β-HSD1 inhibition and better efficacy on anti-obesity than known 11β-HSD1 inhibitor. Therefore, EQST can be developed into anti-obesity candidate compound, and this study may provide more clues for developing higher effective 11β-HSD1 inhibitors.

关键词： EQST Obesity Adipocyte Lipid accumulation Preadipocyte differentiation Adipose tissue 11β-HSD1 11β-HSD1 inhibitor

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道教存思法与《文心雕龙》神思论的生成

江西社会科学 2009年第2期29卷 97-101页

作者：吴崇明南京大学中文系博士研究生南京210093

作为一种精神思维活动,道教"存思"法具有驰骋想象的特点,文学"神思论"的出现,与之密不可分。神思实际上就是把道教的存思方法运用到文学创作中,是文学中的存思。魏晋南北朝时文人化用存思方法进行创作的现象已很普遍,《文心雕龙·神思... 详细信息

作为一种精神思维活动,道教"存思"法具有驰骋想象的特点,文学"神思论"的出现,与之密不可分。神思实际上就是把道教的存思方法运用到文学创作中,是文学中的存思。魏晋南北朝时文人化用存思方法进行创作的现象已很普遍,《文心雕龙·神思》篇的诞生,正是时代发展的必然结果。

关键词：道教存思刘勰《文心雕龙》神思

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核电站核岛施工塔吊布置

施工技术 1999年第8期28卷 47-48页

作者：伍崇明中国核工业第二四建设公司核电工程公司

针对核电站核岛工程中各建筑物平面布置紧凑、现浇混凝土量大、工期紧等特点，提出核岛建造期间施工塔吊动态平面布置、塔吊基础设计及附着装置的安装方法。

关键词：核电站塔式起重机动态平面布置基础附着装置

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不同工况下的泵送混凝土施工技术

建筑技术 2001年第1期32卷 26-27页

作者：伍崇明中国核工业第二四建设公司

秦山核电站二期工程核岛、常规岛工程各建筑物从基础底板到上部结构均为现浇钢筋混凝土结构，其结构复杂、现浇混凝土工程量大、周期长。施工中引进了混凝土泵送设备，包括2台输送泵、2台布料机(32m/28m)、1台32m泵车。1　泵送混凝土施... 详细信息

秦山核电站二期工程核岛、常规岛工程各建筑物从基础底板到上部结构均为现浇钢筋混凝土结构，其结构复杂、现浇混凝土工程量大、周期长。施工中引进了混凝土泵送设备，包括2台输送泵、2台布料机(32m/28m)、1台32m泵车。1　泵送混凝土施工要点　　(1)根据施工方案确定布料机和泵车的位置，布料机要安装牢固，混凝土泵及泵车支脚要平稳牢靠；混凝土输送管沿途要支设牢固，尤其是管子转弯处。浇筑用的管道、混凝土泵、布料机安装就位后，验收符合要求，设备机具检查运转正常。

关键词：泵送混凝土施工技术大体积混凝土