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Acta Pharmacologica Sinica 2007年第1期28卷 81-88页

作者： Qing-song TAO Jian-an REN~2 Jie-shou LI department of General surgery,Jinling Hospital,Clinical School of Nanjing University,Nanjing 210002,china department of General surgery Jinling Hospital Clinical School of Nanjing University Nanjing China

Aim:To examine the inhibitive effects of triptolide on the expression of IL-8,monocyte chemotactic protein(MCP)-1,and matrix metalloproteinases(MMP)-3in subepithelial myofibroblasts(SEMF)stimulated with IL-1β.Methods:SEMFcultures were established from normal colons in patients who underwent gutresection for colorectal *** and MMP-3 expressions weredetermined by ELISA and *** cytosolic amount of phosphorylation ofIκB-α(p-IκB-α)was determined by Western *** DNA binding capacityof NF-κB was evaluated by electrophoretic mobility shift ***:IL-1βstimulated protein and mRNA expression of IL-8,MCP-1,and MMP-3 in *** inhibited these effects of IL-1β in a dose-dependent ***-nistic studies revealed that triptolide markedly decreased IL-1β-induced NF-κBDNA binding capacity and cytosolic amount of p-IκB-α.These results showedthat triptolide inhibited IL-1β-induced chemokine and MMP-3 expression in SEMFthrough the NF-κB ***:Triptolide inhibited IL-1β-inducedchemokine and MMP-3 expression in SEMF by preventing the phosphorylationof IκB-α.

关键词： triptolide chemokine matrix metallopro teinase myofibroblast

Effects of tanshinone on neuropathological changes induced by amyloid β-peptide(1-40)injection in rat hippocampus

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Acta Pharmacologica Sinica 2004年第7期25卷 15-22页

作者： Long-xuan LI2, Jia-pei DAI3, Li-qiang RU4, Guang-fu YIN4, Bin ZHAO department of Neurology, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, china 3The Netherlands Institute for Brain Research, Amsterdam 1105AZ, the Netherlands 4department of Neurobiology, TongjiMedical College of Huazhong Universityof Science and Technology, Wuhan 430030, china department of Neurology Affiliated Hospital Guangdong Medical College Zhanjiang 524001 The Netherlands Institute for Brain Research Zhanjiang 524001 department of Neurobiology Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030

AIM: To investigate the effect of tanshinone (Tan) on the neuropathological changes induced by amyloid β- peptide1-40(Aβ1-40) injection in hippocampus in rats. METHODS: Aβ1-4010 μg was injected bilaterally into the dorsal blade of the dentate gyrus in the hippocampus. The level of acetylcholinesterase (AChE) in hippocampus was evaluated by histochemistry. The expressions of neuronal nitric oxide synthase (nNOS) and inducible form of NOS (iNOS) were detected by immunohistochemistry and Western blot. Aβ1-40-injected rats were treated ig with Tan, the major active ingredient from Salvia miltiorrhiza of Chinese herb extract. RESULTS: The level of AChE positive fibers of each subfield in Aβ1-40-injected hippocampus decreased significantly compared with those of control (P40 injection in hippocampus.

关键词： tanshinone amyloid beta-protein acetylcholinesterase nitric oxide synthase hippocampus

Genistein stimulates osteoblastic differentiation via p38 MAPK-Cbfa1 pathway in bone marrow culture

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Acta Pharmacologica Sinica 2007年第10期28卷 1597-1602页

作者： Qing-chuan LIAO~2,Zhou-sheng XIAO~(4,5),Yan-fang QIN~3,Hong-hao ZHOU~4~2 Affiliated Children's Hospital,Nanjing Medical University,Nanjing 210008,china,~3 department of Pathology,School of Medicine,JinanUniversity,Guangzhou 510632,china ~4 Institute of Clinical Pharmacology,Central South University,Changsha 410078,china Affiliated Children's Hospital Nanjing Medical University Nanjing China Institute of Clinical Pharmacology Central South University Changsha China department of Pathology School of Medicine Jinan University Guangzhou China

Aim:To test the hypothesis that genistein stimulates the osteoblastic differentia-tion through the p38 mitogen activated protein kinase (MAPK)-core-binding fac-tor 1 (Cbfa1) ***:The activation of p38 MAPK was detected byWestern *** phosphatase (ALP) activity and calcium depositionwere assessed for osteoblastic differentiation of bone marrow-derived mesenchy-mal stem cell (BMSC) *** expression of Cbfa1 was analyzed at both themRNA and protein *** activity of Cbfa1 was detected by electrophoreticmobility shift *** sialoprotein (BSP),ALP,osteocalcin (OC),andosteopontin (OPN) gene transcription were also evaluated by either RT-PCR orNorthern ***:Genistein (0.01-1 μmol/L) dose dependently led tothe rapid and sustained activation of the p38 MAPK pathway in mouse *** with genistein (1 μmol/L) resulted in increased ALP activityand calcium deposition of BMSC cultures as a function of *** alsoenhanced Cbfa1 DNA binding activity and promoted the expressions of Cbfa1itself as well as several Cbfa1-regulated genes,including ALP,BSP,OC,and *** treatment with p38 MAPK inhibitor (SB203580) diminished thegenistein-induced osteoblastic maturation and p38 MAPK-Cbfa1 activation inmouse BMSC ***:These results indicated that genistein couldstimulate the osteoblastic differentiation of BMSC cultures through the p38MAPK-Cbfa1 pathway.

关键词： bone marrow-derived mesenchymal stem cells core-binding factor 1 osteoblastic differentiation genistein mitogen-activated protein kinase

Endoscopic pancreatic duct and biliary duct stenting in treatment of chronic pancreatitis with distal benign biliary stricture: a single-center experience

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Hepatobiliary & Pancreatic Diseases International 2011年第5期10卷 539-543页

作者： Ming-Wei Zheng, Ming-Fang Qin and Wang CaiAuthor Affiliations: department of surgery, Tianjin Nankai Hospital, Tianjin 300100, china department of surgery Tianjin Nankai Hospital Tianjin 300100 China

BACKGROUND: The development of endoscopic techniques such as endoscopic retrograde cholangiopancreatography (ERCP), endoscopic sphincterotomy (EST) and stenting are relatively new alternatives to surgery for the treatment of benign lesions in the biliary duct and pancreas. The objective of this study was to assess the value of stenting in the endoscopic pancreatic duct and biliary duct in the treatment of chronic pancreatitis with distal benign biliary stricture. METHODS: Twenty-two patients diagnosed with chronic pancreatitis with distal benign biliary stricture underwent endoscopic treatment in our center, with ERCP, EST, endoscopic retrograde biliary drainage (ERBD) and endoscopic retrograde pancreatic drainage (ERPD) with stents. A numeric rating scale was used to assess pain intensity. The clinical data on endoscopic therapies and recovery of the patients were recorded and compared. RESULTS: ERCPs were successfully performed in 21 patients and 1 (4.5%) failed because of pancreatic ductal variation. A total of 68 ERCPs were performed with 47 pancreatic duct stents and 39 biliary duct stents. The rate of complications was 13.2% (9/68). The abdominal pain score after endoscopic treatment was significantly reduced. The levels of bilirubin and alanine transaminase in all 21 patients were improved compared to those before endoscopic ***: Endoscopic stent drainage of the pancreatic duct and biliary duct for chronic pancreatitis with distal biliary benign stricture can be selected as a safe, effective and minimally invasive therapeutic method.

关键词： endoscopic chronic pancreatitis distal biliary stricture plastic stent

Effect of hepatitis C virus core protein on modulation of cellular proliferation and apoptosis in hilar cholangiocarcinoma

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 71-74页

作者： Ru-Fu Chen, Zhi-Hua Li, Sheng-Quan Zou and Ji-Sheng Chen Guangzhou, china department of Hepatobiliary surgery,Hospital of Zhongshan University, Guangzhou 510120, china department of surgery,Tongji Hospital, Wuhan 430030, china department of Hepatobiliary surgery The Second Affiliated Hospital of Zhongshan University Guangzhou 510120 China. chenrf63@***

BACKGROUND: Hepatitis C virus (HCV) is believed to be an important human pathogen causing carcinoma. But the effect of HCV infection on the alteration of cellular pro- liferation and apoptosis and the relationship between the effect and the development of hilar cholangiocarcinoma are largely unknown. The aim of this study was to assess the effect of HCV core protein on proliferation and apoptosis of hilar cholangiocarcinoma. METHODS: HCV core protein (HCV C protein) was de- tected by peroxidase-antiperoxidase assay in surgical speci- mens from 48 patients with hilar cholangiocarcinoma. The apoptosis index ( AI) and PCNA index ( PI) in hilar cholangiocarcinoma were detected by in situ end labeling assay and streptavidin-biotin assay respectively. RESULTS: The expression of HCV C protein was observed in 32 (67.7%) of the 48 specimens of hilar cholangiocarci- noma. The mean ± standard deviation for AI and PI was 3.52%±0.64% and 46.24%±11.46% respectively. The AI of hilar cholangiocarcinoma specimens with HCV C protein expression was significantly lower than that of HCV C pro- tein negative specimens (P<0.01), whereas the PI of HCV C protein positive specimens was significantly higher than that of HCV C protein negative specimens (P<0.01). CONCLUSION: HCV C protein may promote the cellular proliferation of hilar cholangiocarcinoma and inhibit its cel- lular apoptosis.

关键词： hilar cholangiocarcinoma hepatitis C virus core protein apoptosis proliferation

Tetramethylpyrazine suppresses HIF-1α,TNF-α,and activated caspase-3 expression in middle cerebral artery occlusion-induced brain ischemia in rats

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Acta Pharmacologica Sinica 2007年第3期28卷 327-333页

作者： Yi CHANG~(2,3) George HSIAO~4 Seu-hwa CHEN~4 Yi-cheng CHEN~4 Jiing-han LIN~4 Kuang-hung LIN~4 Duen-suey CHOU~4 Joen-rong SHEU~(4,5)2 department of Anesthesiology,Shin Kong Wu Ho-Su Memorial Hospital,Taipei 110,Taiwan,china 3 School of Medicine,Fu-Jen Catholic University,Taipei,110,Taiwan,china 4 Graduate Institute of Medical Sciences,Taipei Medical University,Tuipei 110,Taiwan,china department of Anesthesiology Shin Kong Wu Ho-Su Memorial Hospital Taipei China School of Medicine Fu-Jen Catholic University Taipei China Graduate Institute of Medical Sciences Taipei Medical University Taipei China

Aim:To examine the detailed mechanisms underlying the inhibitory effect oftetramethylpyrazine(TMPZ)in inflammatory and apoptotic responses inducedby middle cerebral artery occlusion(MCAO)in ***:MCAO-inducedfocal cerebral ischemia in rats was used in this *** hypoxia-induciblefactor-1α(HIF-1α),activation of caspase-3,and TNF-αmRNA transcription inischemic regions were detected by immunoblotting and RT-PCR,***-oxidative activity was investigated using a thiobarbituric acid-reactive sub-stance(TBARS)test in rat brain homogenate ***:We showedthe statistical results of the infarct areas of solvent-and TMPZ(20 mg/kg)-treatedgroups at various distances from the frontal pole in MCAO-induced focal cerebralischemia in *** with TMPZ(20 mg/kg)markedly reduced the infarctarea in all regions,especially in the third to fifth ***-induced focalcerebral ischemia was associated with increases in HIF-lαand the activation ofcaspase-3,as well as TNF-αtranscription in ischemic *** expressionswere markedly inhibited by treatment with TMPZ(20 mg/kg).However,TMPZ(0.5-5mmol/L)did not significantly inhibit TBARS reaction in rat brain ***:The neuroprotective effect of TMPZ may be mediated at least by aportion of the inhibition of HIF-1αand TNF-αactivations,followed by the inhibi-tion of apoptosis formation(active caspase-3),resulting in a reduction in theinfarct volume in ischemia-reperfusion brain ***,TMPZ treatment mayrepresent an ideal approach to lowering the risk of or improving function in is-chemia-reperfusion brain injury-related disorders.

关键词： tetramethylpyrazine middle cerebral artery occlusion HIF-Iα caspase-3 TNF-α thiobarbituric acid-reactive substance

Inhibition of ethanol-induced toxicity by tanshinone ⅡA in PC12 cells

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Acta Pharmacologica Sinica 2006年第6期27卷 659-664页

作者： Xian-fang MENG~2 Xiao-jing ZOU~3 Bin PENG~2 Jing SHI~2 Xin-min GUAN~2 Chun ZHANG~(4,5)2 department of Neurobiology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,china 3 department of Anesthesiology,4 department of Nephrology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022.china department of Neurobiology Tongji Medical College Huazhong University of Science and Technology Wuhan China department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China department of Nephrology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To observe the effects of tanshinone IIA(Tan IIA)on the neurotoxicityinduced by ethanol in PC 12 cells and to explore its protective ***:PC 12cell survival was measured by MTT *** formation of reactive oxygenspecies(ROS)and lactate dehydrogenase(LDH)release were detected by 2′,7′-dichlorofluorescin(DCF)fluorescence and calorimetric method,*** of cell apoptosis was monitored by flow *** expression ofp53 was detected by immuno-fluorescence and flow ***:Ethanolsignificantly impaired the survival of PC12 cells as demonstrated by MTT *** also induced significant ROS formation and increased LDH ***-incubation with Tan IIA in the culture medium significantly reversed these *** caused cell apoptosis and the upregulation of p53 *** anti-apoptosis effects of Tan IIA on ethanol-induced toxicity were accompanied by thedownregulation of pro-apoptotic p53 protein ***:Tan IIAcan protect neurons from apoptosis and might serve as a potential therapeuticdrug for neurological disorders induced by ethanol.

关键词： apoptosis ethanol PC12 cells p53 tanshinone ⅡA

Changes of serum alpha-fetoprotein and alpha-fetoprotein-L3 after hepatectomy for hepatocellular carcinoma: prognostic significance

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Hepatobiliary & Pancreatic Diseases International 2012年第6期11卷 618-623页

作者： Xiao-Feng Zhang, Zheng-Feng Yin, Kui Wang, Zong-Qin Zhang, Hai-Hua Qian,Le-Hua ShiAuthor Affiliations: The 4th department of Hepatic surgery ,Molecular Oncology Laboratory , Eastern Hepatobiliary surgery Hospital, Second Military Medical University, Shanghai 200438, china The 4th department of Hepatic surgery Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China Molecular Oncology Laboratory Eastern Hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200438 China

BACKGROUND: Alpha-fetoprotein (AFP) is the most established tumor marker of hepatocellular carcinoma (HCC), but one of its limitations is non-specificity. Many studies have demonstrated that alpha-fetoprotein-L3 (AFP-L3) is more specific than AFP in the early diagnosis and prognosis of HCC. However, there is a lack of knowledge about the post-hepatectomy profiles of serum AFP and AFP-L3 values in HCC patients. To identify the profiles after surgical resection of HCC, we analyzed the correlation between the profiles and postoperative HCC recurrence or survival, and evaluated their utility in predicting postoperative therapeutic efficacy and prognosis. METHODS: From August 2003 to December 2004, 318 patients with positive serum AFP who had received surgical resections were enrolled in this study. Preoperative and postoperative serum AFP and AFP-L3 levels were measured simultaneously and regularly, and their postoperative profiles during a long term follow-up were recorded and summarized. RESULTS: A high ratio of AFP-L3 to total AFP was shown to correlate with pathologic features of aggressiveness. The overall 1-, 3-, and 5-year recurrence rates of the whole series were 28% 57%, and 84%, and the overall survival rates were 86%, 61% and 33%, respectively. The changes of serum AFP and AFP-L3 after hepatectomy for HCC were classified into 3 groups (group A: AFP-L3 undetectable; group B: AFP-L3 10%). Patients with positive postoperative AFP-L3had significantly earlier recurrence than those with negative results. The overall survival was significantly shorter in the positive groups than in the groups negative for postoperative ***: Post-hepatectomy changes in serum AFP and AFP-L3 levels occurred in three distinct patterns, which were closely correlated with HCC recurrence and patient survival with different prognostic values.

关键词： hepatocellular carcinoma hepatectomy alpha-fetoprotein alpha-fetoprotein-L3

The construction of infinite families of any k-tight optimal and singular k-tight optimal directed double loop networks

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Science china Mathematics 2007年第7期50卷 1055-1064页

作者： Bao-xing CHEN~(1+) Xie-bin CHEN~2 Ji-xiang MENG~3 Wen-jun XIAO~4 1 department of Computer Science,Zhangzhou Teachers College,Zhangzhou 363000,china 2 department of Mathematics and Information Science,Zhangzhou Teachers College,Zhangzhou 363000,china 3 College of Mathematics & System Science,Xinjiang University,Urumqi 830046,china 4 department of Computer Science,South china University of Technology,Guangzhou 510641,china department of Computer Science Zhangzhou Teachers College Zhangzhou China department of Mathematics and Information Science Zhangzhou Teachers College Zhangzhou China College of Mathematics & System Science Xinjiang University Urumqi China department of Computer Science South China University of Technology Guangzhou China

The double loop network(DLN)is a circulant digraph with n nodes and outdegree *** is an important topological structure of computer interconnection networks and has been widely used in the designing of local area networks and distributed *** the number n of nodes,how to construct a DLN which has minimum diameter?This problem has attracted great attention.A related and longtime unsolved problem is:for any given non-negative integer k,is there an infinite family of k-tight optimal DLN?In this paper,two main results are obtained:(1)for any k≥0,the infinite families of k-tight optimal DLN can be constructed,where the number n(k,e,c)of their nodes is a polynomial of degree 2 in e with integral coefficients containing a parameter c.(2)for any k≥0, an infinite family of singular k-tight optimal DLN can be constructed.

关键词： double loop network diameter k-tight optimal singular k-tight optimal 05C12 05C20 05C85

Inhibitory effect of ginsenoside Rb1 on calcineurin signal pathway in cardiomyocyte hypertrophy induced by prostaglandin F2α

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Acta Pharmacologica Sinica 2007年第8期28卷 1149-1154页

作者： Qing-song JIANG~2 Xie-nan HUANG Gui-zhong YANG~4 Xiao-yan JIANG~5 Qi-xin ZHOU~(2,6) ~2department of Pharmacology,Chongqing Medical University,Chongqing 400016,china ~3department of Pharmacology,Zunyi Medical College,Zunyi 563003,china ~4department of Biochemistry Zunyi Medical College,Zunyi 563003,china ~5Chengdu Vocational College of Agricultural Science and Technology,Chengdu 611100,china department of Pharmacology Chongqing Medical University Chongqing China department of Pharmacology Zunyi Medical College Zunyi China department of Biochemistry Zunyi Medical College Zunyi China Chengdu Vocational College of Agricultural Science and Technology Chengdu China

Aim:To examine the antihypertrophic effect of ginsenoside Rb1（Rb1）induced byprostaglandin F2α(PGF2α)in vitro and to investigate the possible mechanismsinvolved in the calcineurin（CAN）signal transduction ***:Thecardiomyocyte hypertrophy induced by PGF2αand the antihypertrophic effect ofRbiwere evaluated in primary culture by measuring the cell diameter,proteincontent,and atrial natriuretic peptide（ANP）mRNA *** and CaNmRNA expressions,CaN and its downstream effectors NFAT3and GATA4 proteinexpressions,and the intracellular free Ca2+concentration([Ca2+]i)were assayedby RT-PCR,Western blot,and fluorescent determination using Fura 2/AM,***:PGF2α（100 nmol/L）significantly increased the cardiomyo-cyte diameter,protein content and[Ca2+]i,and promoted ANP,CaN mRNA,andCaN/NFAT3/GATA4protein expressions,which were inhibited by either Rb1in aconcentration-dependent manner（50,100,and 200μg/mL）or L-arginine（1 mmol/L）.NG-nitro-L-arginine-methyl ester,a nitric oxide synthase inhibitor,could abolishthe effects of L-arginine,but failed to change the effects of Rb1in the ***:The present data implicate that Rb1attenuates cardiachypertrophy,the underlying mechanism may be involved in the inhibition of theCa2+-CaN signal transduction pathway.

关键词： Rb1 prostaglandin F2α cardiac hypertrophy calcineurin