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Coniferyl ferulate,isolated from Radix Angelicae sinensis,reverses multidrug resistance via strong inhibition of GST and down-regulation of P-glycoprotein

中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Chang Chen~1 Xinhua Lu~2 Zhiyong Yan~3 Shaojing Li~1 1 Institute of Chinese Materia Medica china Academy of Chinese Medical sciences Beijing 100700 china 2 New Drug Research and Development Centre of North china Pharmaceutical Group Corporation Hebei 050015 china 3 college of life science and engineering Southwest Jiaotong University Chengdu 610031 china Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700China New Drug Research and Development Centre of North china Pharmaceutical Group Corporation Hebei 0500 college of life science and engineering Southwest Jiaotong University Chengdu 610031 China

P-glycoprotein(P-gp)and glutathione-S-transferase(GST)are key players in multidrug resistance(MDR).Inhibition of the expression of P-gp and/or GST has emerged as a promising therapeutic strategy fo

关键词： coniferyl ferulate Radix Angelicae sinensis (Umbelliferae) multidrug resistance glutathione-S-transferase

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Coniferyl ferulate,isolated from Radix Angelicae sinensis,re...

Theoretical analysis of alloy multiplying factor by the empirical electron theory of solids and molecules

中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Chang Chen~1,Xinhua Lu~2,Zhiyong Yan~3,Shaojing Li~1 1 Institute of Chinese Materia Medica,china Academy of Chinese Medical sciences,Beijing 100700, china 2 New Drug Research and Development Centre of North china Pharmaceutical Group Corporation, Hebei,050015,china 3 college of life science and engineering,Southwest Jiaotong University,Chengdu 610031,china

P-glycoprotein(P-gp)and glutathione-S-transferase(GST)are key players in multidrug resistance(MDR).Inhibition of the expression of P-gp and/or GST has emerged as a promising therapeutic strategy for the reversal of *** ferulate(CF),isolated from Radix Angelicae sinensis(RAS),strongly inhibited human placental *** 50%inhibition concentration (IC50)was 0.3μM,which is greater than an known GST p1-1 inhibitor,ethacrynic acid(EA), using the established HTS *** analysis and computational docking were used to examine the mechanism of inhibition by *** and the expression of P-gp mRNA were evaluated in the MDR positive B-MD-C1(ADR+/+)cell line to investigate the MDR reversal effect of *** results demonstrated dual inhibition of both P-gp expression and GST activity by CF in a concentration-dependent ***,RAS may be a potential source of MDR reversing agents for pharmaceutical use.

关键词： coniferyl ferulate Radix Angelicae sinensis(Umbelliferae) multidrug resistance glutathione-S-transferase

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science china(Technological sciences) 2007年第6期50卷 807-814页

作者： SONG YuePeng1,2, LIU GuoQuan2, LI ZhiLin3, FENG ChengMing1 1 Mechanical and Electronic engineering college, Shandong Agricultural University, Tai’an 271018, china 2 college of Materials science and engineering, University of science and Technology, Beijing 100083, china 3 college of Material science and engineering, Beijing University of Chemical Technology, Beijing 100029, china Mechanical Electronic engineering college Shandong Agricultural University Tai’an 271018 China college of Materials science engineering University of Science Technology Beijing 100083 China college of Material science engineering Beijing University of Chemical Technology Beijing 100029 China

By introducing the distribution probability of structural units in austenite contain- ing alloying elements and considering its effects on phase transformation, this paper establishes a calculation model of distribution probability of structural units. A new valence electron structure (VES) parameter-transformation effect coefficient of alloying elements (HL), is defined and then studied both theoretically and ex- perimentally. The relationship between the parameter HL and the multiplying factor (the quenching capability factor) of alloying elements is studied. The results indi- cate that the two parameters (HL and the quenching capability factor) have the same variation characteristic and substance feature. Therefore, the multiplying factor virtually expresses the relative quantity of structural units in the alloying elements-containing austenite.

关键词： multiplying factor (the quenching capability factor), empirical electron theory of solids and molecules, transformation effect coefficient of alloying elements, structural units in austenite containing alloying elements

Boundary conditions for the bending of a piezoelectric beam

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science china(Physics,Mechanics & Astronomy) 2008年第7期51卷 847-856页

作者： GAO Yang1, XU SiPeng2 & ZHAO BaoSheng3 1 college of science, china Agricultural University, Beijing 100083, china 2 college of engineering, Ocean University of china, Qingdao 266071, china 3 School of Mechanical engineering, University of science and Technology Liaoning, Anshan 114044, china 1. college of science China Agricultural University Beijing 100083 China 2. college of engineering Ocean University of China Qingdao 266071 China 3. School of Mechanical engineering University of Science and Technology Liaoning Anshan 114044 China

For beam bending in transversely isotropic piezoelectric media, the reciprocal theorem and the general solution of piezoelasticity are applied in a novel way to obtain the appropriate stress and mixed boundary conditions accurate to all orders for the beam of general edge geometry and loadings. By generalizing the method developed by Gregory and Wan, a set of necessary conditions on the edge-data for the existence of a rapidly decaying solution is established. The prescribed edge-data of the beam must satisfy these conditions in order that they could generate a decaying state within the beam. When stress and mixed conditions are imposed on the beam edge, these decaying state conditions for the case of bending deformation of piezoelectric beam are derived explicitly. They are then used for the correct formulation of boundary conditions for the beam theory solution (or the interior solution). Besides, an analytical solution of elastic beam is formulated to verify validity of our boundary conditions. For the stress data, our boundary conditions coincide with those obtained in conventional forms of beam theories. More importantly, the appropriate boundary conditions with two sets of mixed edge-data are obtained for the first time.

关键词： boundary conditions beam bending piezoelectric media decaying states regular states

Mixed boundary conditions for piezoelectric plates

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science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 755-761页

作者： GAO Yang 1 ,XU SiPeng 2 &ZHAO BaoSheng 3 1college of science,china Agricultural University,Beijing 100083,china 2college of engineering,Ocean University of china,Qingdao 266071,china 3 School of Mechanical engineering,University of science and Technology of Liaoning,Anshan 114044,china college of science China Agricultural University Beijing 100083 China college of engineering Ocean University of China Qingdao 266071 China School of Mechanical engineering University of Science and Technology of Liaoning Anshan 114044 China

For plate bending and stretching problems in piezoelectric materials,the reciprocal theorem and the general solution of piezoelasticity are applied in a novel way to obtain the appropriate mixed boundary conditions accurate to all order.A decay analysis technique is used to establish necessary conditions that the prescribed data on the edge of the plate must satisfy in order that it should generate a decaying state within the *** the case of axisymmetric bending and stretching of a circular plate,these decaying state conditions are obtained explicitly for the first time when the mixed conditions are imposed on the plate *** are then used for the correct formulation of boundary conditions for the interior solution.

关键词： boundary conditions piezoelectric plates mixed data bending problems stretching problems

pH-Dependent Stability of EGX, A Multi-functional Cellulase from Mollusca, Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 603-608页

作者： Wen-Ying LI2, Ji WANG1, Yan-Hong LI1, Ming DING1, Gen-Jun XU1,3, Lan-Ying LIU2, and Fu-Kun ZHAO1,3* 1Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, the Chinese Academy of sciences,Graduate School of the Chinese Academy of sciences, Shanghai 200031, china 2college of life science, Jilin University, Changchun 130023, china college of life science, Zhejiang University of science and Technology, Hangzhou 310018, china Key Laboratory ofProteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

The cellulase activity and stability of EGX, a multi-functional cellulase previously purified from the mollusca Ampullaria crossean, was systematically studied under different pH. The pH induced con- formation and stability change of EGX have been investigated by using the intrinsic fluorescence, ANS fluorescence and CD spectrum. It has been found that the conformation and activity of this cellulase were strongly dependent on the pH. EGX was stable for both the enzyme activity and the conformation from pH 5.6 to pH 7.4. As shown by intrinsic and ANS fluorescence, no red shift of emission maximum occurred and a negligible intensity change was observed at pH 5.6–7.4. The activity of EGX remained about 80% in pH 5.6–7.4 and obviously decreased out of side the pH range. Urea-induced changes in EGX at pH 5.4 and pH 8.0 were measured by intrinsic fluorescence and CD spectrum. At pH 5.4, a significantly red shift of emission maximum occurred when the concentration of urea was 5 M compared to the concentration was 3 M at pH 8.0. The α-helix at pH 5.4 was 40.51% in the absence of urea and 31.04% in the presence of 4 M urea. At pH 8.0 the α-helix was 7.23% in the presence of 4 M urea. The data indicated that EGX was much susceptible to urea-induced unfolding at pH 8.0 and much stable at pH 5.4. The greater pH dependent stability of EGX may allow the enzyme to adequately catalyze the hydrolysis of cellulosic materials under natural or industrial extreme conditions.

关键词： multi-functional cellulase stability Ampullaria crossean fluorescence spectroscopy pH-dependent structural change CD spectrum

Diversity and ecological distribution of endophytic fungi associated with medicinal plants

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science china(life sciences) 2008年第8期51卷 751-759页

作者： SUN JianQiu1,2,3, GUO LiangDong1, ZANG Wei2,3, PING WenXiang4 & CHI DeFu3 1 Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of sciences, Beijing 100101, china 2 college of life science and engineering, Qiqihar University, Qiqihar 161006, china 3 college of Forestry, Northeast Forestry University, Harbin 150040, china 4 college of life science, Heilongjiang University, Harbin 150080, china 1. Systematic Mycology and Lichenology Laboratory Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China 2. college of life science and engineering Qiqihar University Qiqihar 161006 China 3. college of Forestry Northeast Forestry University Harbin 150040 China 4. college of life science Heilongjiang University Harbin 150080 China

A total of 973 isolates of endophytic fungi were recovered from 1144 tissue fragments of the six me-dicinal plant species belonging to 4 families collected in the Beijing Botanical Garden. Of these isolates 778 sporulated and were identified into 21 taxa by morphological characteristics. Among the taxa 11 belonged to Coelomycetes, 6 to Ascomycetes, and 4 to Hyphomycetes. Various numbers of endophytic fungi (5―8 taxa) were obtained from each plant. Alternaria alternata was the dominant species in the 6 plants, and Microsphaeropsis conielloides was also dominant in Eucommia ulmoides. There were high colonization rates (47.9%―63.1%) and isolation rates (0.7―0.93) of endophytic fungi, and they were conspicuously higher in twigs than those in leaves in the 6 plants examined. The colonization and isolation rates of endophytic fungi increased with the twig age. The results based on the analyses of cluster and Sorenson's similarity coefficients indicated that some endophytic fungi showed a certain degree of host and tissue preference.

关键词： endophytic fungus medicinal plant diversity host and tissue preference

Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(life sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry science,Beijing 100097,china 2 Department of Chemistry and Chemical engineering,Hunan Institute of engineering,Xiangtan 411104,china 3 college of life science,Capital Normal University,Beijing 100037,china 4 college of life science,Zhongkai University of Agriculture and Technology,Guangzhou 510225,china 5 college of Grassland science,Gansu Agricultural University,Lanzhou 730070,china 6 Department of Biology,Henan Institute of science and Technology,Xinxiang 453003,china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical engineering Hunan Institute of Engineering Xiangtan 411104 China 3. college of life science Capital Normal University Beijing 100037 China 4. college of life science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. college of Grassland science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay

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Acta Oceanologica Sinica 2009年第2期28卷 121-126页

作者： CHEN Jie ZHEN Yu MI Tiezhu YU Zhigang college of Marine life science Ocean University of China Qingdao 266003 China college of Environmental science and engineering Ocean University of China Qingdao 266100 China college of Chemistry and Chemical engineering Ocean University of China Qingdao 266100 China

Prorocentrum donghaiense is an important harmful algae bloom （HAB） causing creature in china＇s seas, and the conventional visual detection can not cope with long-term monitoring and highthroughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay （NPA-SH） was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, and the regression equation is y=4× 10^- 6x＋ 0.694 9, in which x is the cell number, and y is the optical density, with r2=0.953 5. These results show that the NPA-SH method has good feasibility in the detection of P. donghaiense. Results of NPA-SH and microscopy are excellent for each sample. The NPA-SH method was a simple way in quantitative detection of P. donghaiense, and the whole process could be finished in about six hours, which provided a new approach in high-throughput sampling and long-term monitoring of P. donghaiense.

关键词： Prorocentrum donghaiense ribosomal RNA S1 enzyme sandwich hybridization integrated with nuclease protection assay (NPA-SH)

Effect of lead on survival, locomotion and sperm morphology of Asian earthworm, Pheretima guillelmi

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Journal of Environmental sciences 2009年第5期21卷 691-695页

作者： ZHENG Rongquan, LI Canyang college of Chemistry and life science, Zhejiang Normal University, Jinhua 321004, china. [a]college of Chemistry and life science Zhejiang Normal University Jinhua 321004 China

To provide basic toxicity data for formulating risk characterization benchmarks, the effects of lead on survival, locomotion, and sperm morphology were investigated in the Asian earthworm Pheretima guillelmi. The LC50 of P. guiUelmi for 7 and 14 d were 4285＋339 mg/kg and 3207＋248 mg/kg, which shows P. guillelmi can tolerate a higher concentration of lead nitrate. The average weight of the surviving earthworms decreased at concentration of 2800 mg Pb/kg soil, and the locomotor ability of earthworms exposed to a range of soil Pb concentrations showed a general decrease with increasing Pb concentrations. We also presented data depicting the sperm morphology of earthworms, which shows potential as a sensitive biomarker for measuring the effects of heavy metal on reproduction.

关键词： lead Pheretima guillelmi sperm morphology locomotion