检索结果-南通市图书馆

The cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from Spider Ornithoctonus huwena

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第10期36卷 656-660页

作者： Peng QIAO, Xiao-Pan ZUO, Zhi-Fang CHAI, and Yong-Hua JI* institute of Physiology, Shanghai institutes for biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china institute of Physiology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

In present study the full-length cDNA of a novel toxin from the venom gland of spider Ornithoctonus huwena, named as SHT-I, has been cloned using the strategy of rapid amplification of cDNA ends, and then the whole genomic sequence of SHT-I (Selenocosmia huwena toxin-I) was determined using sequence-specific primers synthesized based on the acquired 3’ and 5’ ends of SHT-I cDNA sequence. It is unexpectedly found that intron was lacking in the genomic sequence of SHT-I. The result might evoke an interesting question whether the gene code of inhibitor cystine-knot peptides from spider venom is distinct from that of those known toxic peptides of scorpion and cone snail.

关键词： Ornithoctonus huwena spider cDNA genomic DNA peptide toxin ICK motif

Crystallization and preliminary X-ray studies of porcine kidney aminoacylase Ⅰ

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1997年第20期42卷 1750-1752页

作者： ZHAOHaiyan, MENGWuyi, LIN Zhengjiong and ZHOU Haimeng1. National Laboratory of Biomacromolecules, institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, china 2. Department of biological Science and Biotechnology, Tsinghua University, Beijing 100084, china National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China Department of biological Science and Biotechnology Tsinghua University Beijing China

AMINOACYLASE Ⅰ（ACY-1）（E. C. 3. 5. 1. 14 ） participated in amino acid metabolism existingin mammalian kidney and microorganisms, and catalyzes reversible hydrolysis of acylaminoacids. ACY-1 consists of 772 amino acids, and the molecular weight is 85 500 daltons. It is adimeric metalloprotein having two Zn2+ in the molecule. The nucleotide and the amino acid se-quence of porcine and human ACY-1 have been determined. The nucleotide sequence and

关键词： aminoacylase I crystallization preliminary crystallography.

Recent progress in neurodegenerative disorder research in china

在线全文

学校读者我要写书评

暂无评论

Science china(Life Sciences) 2010年第3期53卷 348-355页

作者： ZHOU JiaWei institute of Neuroscience,Shanghai institutes for biological Sciences,Chinese Academy of Sciences,Shanghai,200031 1. institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Neurodegenerative disorders,including Alzheimer's disease(AD) and Parkinson's disease(PD),are common disorders of the central nervous system among aging *** the last 10 years insights concerning the etiology,diagnosis and pathogenesis of these diseases have come from research carried out by Chinese *** findings include the description of Chinese patients with autosomal recessive early-onset PD,the function of the tau protein,molecular mechanisms underlying protein aggregation,and the identification of biomarkers for AD diagnosis and molecules/compounds with potential neuroprotective activities.

关键词： neurodegenerative disorders pathogenesis diagnosis neuroprotection Alzheimer’s disease Parkinson’s disease

Performance prediction and flow analysis in the vaned distributor of a pump turbine under low flow rate in pump mode

在线全文

学校读者我要写书评

暂无评论

中国科学：技术科学英文版 2010年第12期53卷 3302-3309页

作者： YIN JunLian1, LIU JinTao1, WANG LeQin1, JIAO Lei1, WU DaZhuan1 & QIN DaQing2 1 institute of Chemical Machinery, Department of Chemical and biological Engineering, Zhejiang University, Hangzhou 310027, china 2 Harbin institute of Large Electrical Machinery, Harbin 150001, china 1. institute of Chemical Machinery Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China2. Harbin Institute of Large Electrical Machinery Harbin 150001 China

The main goal of this work is to investigate the possible different flow patterns existing in pump turbine under off-design conditions in pump mode. Numerical simulations by solving the Navier-Stokes equation, coupled with the "SST k-ω" turbulence model, were carried out. Flow characteristics were assumed to be stalled in the appropriate region of ?ow rate levels of Q/QD=0.15–0.61. The simulation result was compared with experimental data and they showed good agreement. Consequently, velocity fields in three axial locations in stay vanes and guide vanes were analysed in details. It was shown that "jet-wake" flow pattern exists near the band, which changes little in the whole shape with flow rate increasing; to the middle location of vanes, reverse flow begins to appear on the interface between the runner and guide vanes, which will disappear gradually as the flow rate increases; massive reverse flow is captured near the crown, whose intensity will be weakened as the flow rate increases. Ultimately, it was found that the special head-flow profile can be ascribed to the special hydraulic loss characteristics of the stay vanes and guide vanes.

关键词： pump turbine flow simulation stalled flow guide vane

An Effective Method for Raising Antisera Against β-defensins: Double-copy Protein Expression of mBin1b in E. coli

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第8期36卷 571-576页

作者： Li-Qing XIAO, Ai-Hua LIU, and Yong-Lian ZHANG State Key Laboratory of Molecular Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

Bin1b is a rat epididymis specific β-defensin which may have fertility related functions in addition to its antimicrobial activity. β-defensins are cysteine-rich cationic antimicrobial peptides that have their important implications in innate and adaptive immunity. Though considerable numbers of new β-defensins have been discovered, few corresponding antibodies have been reported. The small peptide with special structure and antimicrobial nature of β-defensins make them very difficult to express in prokaryotic system. Here we adopted a double-copy protein expression scheme based on which not only the mBin1b protein was successfully expressed but also the immunity of the antigen was enhanced. The validity of the antisera was verified by using Western blotting and immunohistochemical analyses. It will be a useful tool for deeply investigating the roles of Bin1b and also provide a simple but effective method in raising antisera against other members of the β-defensin gene family.

关键词： Bin1b epididymis β-defensin double-copy protein polyclonal antiserum

Functional Analysis of Multiple Transcription Factor Sites in a Regulatory Element of Human ε-Globin Gene

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第10期36卷 673-680页

作者： Chun-Hui HOU, Jian HUANG, and Ruo-Lan QIAN* State Key Laboratory of Molecular Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

The developmental control of the human ε-globin gene expression is mediated by transcrip- tional regulatory elements in the 5’ flanking DNA of this gene. A previously identified negative regulatory element (–3028 to –2902 bp, termed ε-NRAII) was analyzed and one putative NF-κB site and two GATA sites locate at –3004 bp, –2975 bp and –2948 bp were characterized. Electrophoresis mobility shift assay (EMSA) showed that the putative NF-κB site was specifically bound by nuclear proteins of K562 cells. Data obtained from transient transfection showed that the expression of reporter gene could be upregulated about 50% or 100% respectively when ε-NRAII was inserted upstream of the SV40 promoter or ε-globin gene proximal promoter (–177 bp to +1 bp), suggesting that ε-NRAII might not be a classic silencer. Mutation in the putative NF-κB site or in the GATA site (at –2975 bp) slightly reduced the expression of reporter gene driven by SV40 promoter or ε-globin gene proximal promoter. However, the mutation of GATA site at –2948 bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. Further mutation analysis showed that the negative effect of mutation in GATA site at –2948 bp on SV40 promoter was not affected by the mutation of the putative NF-κB site, whereas it could be abolished by the mutation of GATA site at –2975 bp. Furthermore, the mutation of both GATA sites could synergistically reduce the reporter gene activity driven by ε-globin gene proximal promoter. Those results suggested that ε- NRAII might function differently on the SV40 promoter and ε-globin gene proximal promoter.

关键词： ε-globin gene regulatory element NF-κB GATA

Transgenic mice overexpressingγ-aminobutyric acid transporter subtypeⅠ develop obesity

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2000年第4期10卷 303-310页

作者： MA YING HUA JIA HUA HU XIAO GANG ZHOU RUO WANG ZENG ZHEN TONG MEI JIAN FEI LI HE GUO institute of Biochemistry and Cell Biology, Shanghai institutes for biological Sciences, Chinese Academy of Science, Shanghai 200031, china Shanghai institute of harmacy.ankara.edu.tr

Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity.

关键词： γ-aminobutyric acid transporter subtype Ⅰ transgenic mouse obesit€

Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2002年第1期12卷 55-62页

作者： Ru GANG ZHANG, Ru PING ZHANG, XING WANG WANG, HONG XIE Department of Biotherapy, institute of Biochemistry and Cell Biology, Shanghai institutes for biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, china 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China

Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 1uM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G2/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process.

关键词： Telomerase telomere cisplatin hepatocellular carcinoma cell cycle cell growth.

MicroRNA in cell differentiation and development

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Life Sciences) 2009年第3期52卷 205-211页

作者： SHI Yi & JIN YouXin State Key Laboratory of Molecular Biology,Shanghai institute of Biochemistry and Cell Biology,Shanghai institutes for biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china State Key Laboratory of Molecular Biology Shanghai Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The regulation of gene expression by microRNAs(miRNAs) is a recently discovered pattern of gene regulation in animals and *** have been implicated in various aspects of animal development and cell differentiation,such as early embryonic development,neuronal development,muscle development,and lymphocyte development,by the analysis of genetic deletions of individual miRNAs in *** studies show that miRNAs are key regulators in animal development and are potential causes of human *** we review some recent discoveries about the functions of miRNAs in cell differentiation and development.

关键词： miRNA embryonic development neuronal development muscle development lymphocyte development