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短指软珊瑚(Sinularia acuta)热休克蛋白HSP70家族特征及进化分析

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热带海洋学报 2024年第4期43卷 123-136页

作者：李子若罗晏杰曹政 chin yaoxian 王沛政海南热带海洋学院崖州湾创新研究院海南三亚572022 海南南沙珊瑚礁生态系统国家野外科学观测研究站广东广州510300 海南热带海洋学院生态环境学院海南三亚572022 三亚海昌梦幻海洋不夜城海南三亚572022 海南省现代化海洋牧场工程研究中心海南三亚572022 海南省近岸海洋生态环境过程与碳汇重点实验室海南三亚572022

热休克蛋白70(heat shock protein 70, HSP70)在生物细胞或组织免受热或氧化应激等方面起着关键作用,是已知高度保守的蛋白质之一。由于全球环境持续升温,珊瑚大面积白化、死亡,珊瑚如何应对持续升温的抗逆机制是科学研究热点。本研究... 详细信息

热休克蛋白70(heat shock protein 70, HSP70)在生物细胞或组织免受热或氧化应激等方面起着关键作用,是已知高度保守的蛋白质之一。由于全球环境持续升温,珊瑚大面积白化、死亡,珊瑚如何应对持续升温的抗逆机制是科学研究热点。本研究从高温胁迫短指软珊瑚测序蛋白序列数据库分析鉴定出了28个HSP70蛋白家族成员,均为酸性亲水蛋白,大部分蛋白质结构较为稳定。亚细胞定位表明HSP70蛋白主要分布在珊瑚细胞核、细胞质中,在线粒体、内质网上也有少量分布。信号肽预测表明, 28个HSP70蛋白成员中26个没有信号肽,大部分不属于分泌蛋白,不存在跨膜结构。系统进化树结果表明短指软珊瑚HSP70蛋白家族成员聚成5大类。短指软珊瑚HSP70蛋白家族结构和保守基序分析中预测到了10条保守基序motif分为5个亚族。短指软珊瑚HSP70蛋白家族二级结构主要以α-螺旋和无规则卷曲为主,α-螺旋的含量占比大。28个HSP70家族蛋白中有25个预测到了N-糖基化位点,且位点个数在1~9范围内。28个HSP70家族蛋白均预测到磷酸化位点和O-糖基化位点,总个数分别在41~96和1~23范围内。本研究HSP70家族蛋白结果为今后珊瑚在应对全球升温胁迫中的适应机制等方面研究奠定了基础。

关键词：短指软珊瑚热休克蛋白 HSP70家族进化

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光胁迫下一种短指软珊瑚（Sinularia sp.）的白化响应研究

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应用海洋学学报 2024年

作者：朱乐沈贝祺曹政齐钊 chin yaoxian 王沛政海南热带海洋学院崖州湾创新研究院海南热带海洋学院海南南沙珊瑚礁生态系统国家野外科学观测研究站三亚海昌梦幻海洋不夜城海南大学

软珊瑚是海洋中最普遍的低等无脊椎动物之一，是海洋天然产物及海洋药物研究的热点生物种类。但软珊瑚与造礁石珊瑚均会因环境胁迫以及病害发生白化，目前珊瑚白化研究几乎都以造礁石珊瑚为研究对象，而对软珊瑚的研究极少。本研究通过... 详细信息

软珊瑚是海洋中最普遍的低等无脊椎动物之一，是海洋天然产物及海洋药物研究的热点生物种类。但软珊瑚与造礁石珊瑚均会因环境胁迫以及病害发生白化，目前珊瑚白化研究几乎都以造礁石珊瑚为研究对象，而对软珊瑚的研究极少。本研究通过对比不同白化状态下短指软珊瑚（Sinularia sp.）的虫黄藻密度及多样性、共生细菌多样性、转录组表达等差异，初步研究了短指软珊瑚的白化适应机制。结果表明，短指软珊瑚遮光处理20天左右开始颜色变淡，30天左右完全白化，并无死亡，表现出一定的环境适应性。其白化状态的虫黄藻密度较对照组减少约98.69%，而半白化短指软珊瑚则减少约95.40%，虫黄藻密度下降会导致短指软珊瑚能量获取能力下降，从而会对短指软珊瑚各种生理功能产生影响。与正常短指软珊瑚相比，白化和半白化短指软珊瑚的虫黄藻α多样性和丰度均降低。短指软珊瑚白化后细菌α多样性变低，属于γ-变形菌纲的Endozoicomonas成为短指软珊瑚共生细菌群落中的优势物种。对比不同白化状态下短指软珊瑚转录组数据，发现短指软珊瑚在白化过程中差异表达上调基因数目增加，而差异表达下调基因数目减少。代谢通路基因分析显示，白化后其蛋白消化与吸收相关的基因表达持续上升，而碳水化合物消化与吸收相关的基因表达显著下降，这些变化可能与白化珊瑚异养营养方式有关。以上结果初步阐述了光照胁迫下短指软珊瑚白化适应性机制，可为今后进一步探索软珊瑚白化适应性机制提供借鉴。

关键词：白化生理差异转录组软珊瑚虫黄藻共生细菌群落多样性

Inhibition of citral nanoemulsion to growth,spoilage ability and Al-2/luxS quorum sensing system of Shewanella putrefaciens CN-32:a study on bacteriostasis from in vitro culture and gene expression analysis

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Food Quality and Safety 2022年第3期6卷 435-445页

作者： Zhiheng Hu yaoxian chin Jiayin Huang Jiaying Zhou Gaoshang Li Yaqin Hu Chunhong Yuan Jianchu Chen College of Biosystems Engineering and Food Science National-Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agro-Food ProcessingIntegrated Research Base of Southern Fruit and Vegetable Preservation TechnologyFuli Institute of Food ScienceZhejiang UniversityHangzhouChina College of Food Science and Engineering Hainan Tropical Ocean UniversityYazhou Bay Innovation InstituteMarine Food Engineering Technology Research Center of Hainan ProvinceCollaborative Innovation Center of Marine Food Deep ProcessingSanyaChina Department of Food Production and Environmental Management Faculty of AgricultureIwate UniversityUeda4-3-5MoriokaJapan

Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality *** and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was *** growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/*** of membrane *** and biofilm formation suggested significant biological structure damage in bacteria after ***,the Qs signaling molecule Al-2 content showed a decline with increasing treatment *** sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying ***:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of *** synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.

关键词： Shewanella putrefaciens citral quorum sensing system gene expression analysis

Docosahexaenoic acid-rich fish oil prevented insulin resistance by modulating gut microbiome and promoting colonic peptide YY expression in diet-induced obesity mice

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Food Science and Human Wellness 2022年第1期11卷 177-188页

作者： Wanxiu Cao Fang Liu Robert WLi yaoxian chin Yuming Wang Changhu Xue Qingjuan Tang College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Drugs Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao 266003China United States Department of Agriculture Agriculture Research Service(USDA-ARS)Animal Genomics and Improvement LaboratoryBeltsvilleMD 20705USA Hainan Tropical Ocean University Sanya 572022China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266003China

It is unclear how docosahexaenoic acid(DHA)improves insulin resistance via modulating gut microbiome in obese *** used diet-induced obesity(DIO)mice as a model to study the effects of DHA-rich fish oil(DHA-FO)on host metabolic disorders and colonic ***-FO reduced fat deposition,regulated lipid profiles and alleviated insulin resistance in DIO *** because DHA-FO prevented the permeation of lipopolysaccharide across intestinal epithelial barrier,and promoted peptide YY(PYY)secretion via the mediation of short chain fatty acids receptor(FFAR2)in ***,DHA-FO might regulate PYY expression by reversing microbial dysbiosis,including increasing the abundance ofAkkermansia muciniphila and Lactobacillus,and suppressing the growth of ***-FO also altered gut microbial function(e.g."linoleic acid metabolism")associated with PYY expression(r>0.80,P<0.05).Herein,DHA-FO enhanced insulin action on glucose metabolism by altering gut microbiome and facilitating colonic PYY expression in DIO mice.

关键词： Docosahexaenoic acid Diet-induced obesity Insulin resistance Peptide YY Gut microbiome

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除草剂乙草胺胁迫下两种软珊瑚生理响应

热带海洋学报 2023年第4期42卷 133-145页

作者：郁强董娴娴李长青张欣赵国理 chin yaoxian 王沛政海南省近岸海洋生态环境过程与碳汇重点实验室海南三亚572022 海南热带海洋学院崖州湾创新研究院海南三亚572022 海南三亚国家级珊瑚礁自然保护区管理处海南三亚572000

文章以乙草胺(acetochlor,ACT)为环境胁迫因子,利用不同浓度的乙草胺对肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)进行长期和短期胁迫,以研究胁迫下的珊瑚生理指标变化情况。研究结果显示,肉质软珊瑚和短指软珊瑚受到不... 详细信息

文章以乙草胺(acetochlor,ACT)为环境胁迫因子,利用不同浓度的乙草胺对肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)进行长期和短期胁迫,以研究胁迫下的珊瑚生理指标变化情况。研究结果显示,肉质软珊瑚和短指软珊瑚受到不同浓度乙草胺胁迫后,珊瑚的叶绿素a和虫黄藻含量呈现先升高后降低的趋势,超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性持续升高然后降低并保持低活性水平,碱性磷酸酶(alkaline phosphatase,AKP)活性先升高后逐渐降低,谷胱甘肽巯基转移酶(glutathione s-transferase,GST)活性升高并保持在较高水平。胁迫后肉质软珊瑚的共生虫黄藻多样性变低,而短指软珊瑚的共生虫黄藻则是丰富度变低。丰度高的虫黄藻都属于系群C,而短指软珊瑚G系群丰度升高。胁迫后软珊瑚Cyanobacteria(蓝细菌)丰度逐渐增加,Firmicutes(厚壁菌门)丰度逐渐减少。以上结果表明,肉质软珊瑚(Sarcophyton sp.)和短指软珊瑚(Sinularia sp.)在受到乙草胺胁迫后,其珊瑚生理指标产生了较大变化。

关键词：软珊瑚乙草胺虫黄藻生理指标响应机制

基于EF-1α和β微管蛋白基因序列的棉花枯萎病菌遗传多样性和单倍型分析

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棉花学报 2023年第4期35卷 334-344页

作者：王海莹陈小海钟烨仪龚举武刘平 chin yaoxian 王沛政袁有禄中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室河南安阳455000 海南热带海洋学院海南省近岸海洋生态环境过程与碳汇重点实验室海南三亚572022 海南热带海洋学院崖州湾创新研究院海南三亚572022

【目的】通过分析棉花枯萎病菌的遗传多样性,探究新疆棉花枯萎病菌株的分群及其演化。【方法】2022年在新疆不同植棉区共分离出22株棉花枯萎病菌株,对延伸因子1α(elongation factor-1α,EF-1α)和β微管蛋白基因进行扩增、测序,并从美... 详细信息

【目的】通过分析棉花枯萎病菌的遗传多样性,探究新疆棉花枯萎病菌株的分群及其演化。【方法】2022年在新疆不同植棉区共分离出22株棉花枯萎病菌株,对延伸因子1α(elongation factor-1α,EF-1α)和β微管蛋白基因进行扩增、测序,并从美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库获取36个棉花枯萎病菌株的相关基因序列信息。基于上述基因序列分别进行系统进化分析和单倍型分析。【结果】基于57条EF-1α基因序列的进化树分析表明,棉花枯萎病菌可分为3大群,第1大群包含来自新疆、河北和澳大利亚的共31个枯萎病菌株,该大群可分成4个亚群;第2大群包含25个枯萎病菌株,构成比较复杂,可分成3个亚群;第3大群仅包含美国菌株LA140。基于28条β微管蛋白基因序列的进化树分析表明,本次分离的新疆棉花枯萎病菌株与棉花枯萎病菌7号和8号生理小种不同。根据EF-1α基因序列构建的单倍型网络将棉花枯萎病菌株分为19个单倍型,新疆21个棉花枯萎病菌株归属于有共同起源的5种单倍型。【结论】本研究分离的新疆棉花枯萎病菌株与已报道的棉花枯萎病菌1~8号生理小种均不相同,但与河北菌株的亲缘关系较近。EF-1α单倍型分析表明,本研究中的所有棉花枯萎病菌均从1号生理小种演化而来。

关键词：棉花枯萎病菌单倍型分析遗传多样性分析延伸因子1α β微管蛋白

High glucose reduces Nrf2-dependent cRAGE release and enhances inflammasome-dependent IL-1βproduction in monocytes:the modulatory effects of EGCG

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Food Science and Human Wellness 2024年第3期13卷 1531-1542页

作者： Chi-Hao Wu Yin-Hsuan Chang chin-Lin Hsu Sheng-Yi Chen Gow-chin Yen Graduate Programs of Nutrition Science School of Life ScienceTaiwan Normal UniversityTaipei 106209TaiwanChina Department of Food Science and Biotechnology Chung Hsing UniversityTaichung 40227TaiwanChina School of Nutrition Chung Shan Medical UniversityTaichung 40201TaiwanChina

Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human ***,the molecular mechanisms underlying sRAGE remain *** this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)*** modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also *** enhanced intracellular reactive oxygen species(ROS)generation and RAGE *** secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear ***,the HG effects were counteracted by siRAGE and exacerbated by *** immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this *** data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating *** HG conditions,the treatment of EGCG reduced ROS generation and RAGE ***-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 *** inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.

关键词： Epigallocatechin gallate(EGCG) Inflammasome Nuclear factor erythroid 2-related factor 2(Nrf2) Receptor for advanced glycation end products(RAGE) Soluble RAGE(sRAGE)

Regulation of aging by NELF-A and RNA polymerase Ⅱ elongation

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Neural Regeneration Research 2024年第7期19卷 1415-1416页

作者： chin-Tong Ong Zhen-Kai Ngian Temasek Life Sciences Laboratory and Department of Biological Sciences National University of SingaporeSingaporeSingapore Department of Genetics Harvard Medical SchoolBostonMAUSA Institute of Molecular and Cell Biology Agency for ScienceTechnology and ResearchSingaporeSingapore

Epigenetic regulation of aging:Aging is defined as the gradual decline of physiological function and cellular integrity,causing o rganismal vulnerability to age-onset diseases and *** in different animal models have l... 详细信息

关键词： aging twelve integrity

Z-shaped incision without epithelial resection in pterygium surgery

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International Journal of Ophthalmology(English edition) 2024年第7期17卷 1262-1266页

作者： Satoru Kase Shinki chin Susumu Ishida Department of Ophthalmology Faculty of Medicine and Graduate School of MedicineHokkaido UniversitySapporo 060-8638Japan

AIM:To introduce a novel surgical technique using a Z-shaped incision without epithelial resection in ophthalmic ***:This was a prospective *** pterygium surgery,all proliferative tissues were separated from the cornea and conjunctiva without resection of the *** unaffected conjunctiva was incised in a *** upper(or lower)conjunctival flap was sutured to the lower(or upper)normal conjunctiva on the limbal sclera,while the proliferative tissue was sutured to the upper conjunctiva(or lower)near the ***:Ten patients with pterygia were eligible for this *** patients with primary pterygia and 2 with recurrent pterygia were *** age of patients at surgery ranged from 47 to 90y(average:71.9y).Five patients each showed right and left-sided *** postoperative follow-up periods were from 8 to 78mo(average:25.0mo).The surgery was successfully conducted and wounds were favorably reconstructed in all *** proliferative tissues sutured to the normal conjunctiva showed palor and attenuated neovessles,and never showed re-growth after *** patients did not show *** pterygium was noted in 1 patient,but additional treatments were not ***:The procedure involves the reconstruction of pterygial tissue and normal conjunctiva using a Z-shaped *** scleral limbal wound can be covered with nonaffected conjunctiva without any excision of conjunctival epithelia in patients with primary or recurrent pterygia.

关键词： pterygium surgery Z-shape non-resection