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ATP translocation and chloroplast biology

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National Science Review 2019年第6期6卷 1073-1076页

作者： chia P.Voon boon L.Lim School of Biological Sciences University of Hong Kong

By studying changes in ATP levels in the plastids and cytosol of live plants using a Mg ATP2-specific F?rster resonance energy transfer（FRET）-based sensor, the entry of cytosolic ATP to the mature chloroplasts of Ar... 详细信息

关键词： ATP translocation and chloroplast biology

A cost-benefit analysis for the implementation of riparian buffer strips in the Shihmen reservoir watershed

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International Journal of Sediment Research 2011年第3期26卷 393-399页

作者： chia-Ling CHANG Ying-Sung HSU Bing-Jean LEE Li-Jung weng Department of Water Resources Engineering and Conservation Feng Chia University No.100 Wenhwa Rd. SeatwenTaichung40724TaiwanChina 3. No.100 Wenhwa Rd.SeatwenTaichung40724TaiwanChina Department of Civil Engineering Feng Chia University

The Shihmen reservoir is an important water source for about 3.4 million people in northern Taiwan. To protect reservoir water quality,it is necessary to conserve and manage the associated watersheds. Riparian buffer strips can trap pollutants emitted near a *** location and design of a buffer strip can influence its pollutant-trapping *** commitment of land for use as a riparian buffer strip must consider the project’s economic *** present research is a cost-benefit analysis of various possible land developments in the Shihmen reservoir *** study has applied a regression equation to evaluate pollutant-trapping efficiency levels of riparian buffer strips of various *** buffer strips have been evaluated in terms of net economic effectiveness and benefit-cost *** indicate that the optimal buffer strip width is 30 m for the Shihmen reservoir watershed.

关键词： Buffer strip Cost-benefit analysis Watershed conservation

Involvement of P53 and Bax/Bad triggering apoptosis in thioacetamide-induced hepatic epithelial cells

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World Journal of Gastroenterology 2006年第32期12卷 5175-5181页

作者： Li-Hsuen Chen chia-Yu Hsu Ching-Feng weng Institute of Biotechnology National Dong Hwa University HualienTaiwanChina

AIM： Thioacetamide （TAA） has been used in studying liver fibrosis and cirrhosis, however, the mechanisms of TAA-induced apoptosis in liver are still unclear. The hepatic epithelial cell line clone 9 was cultured and treated with TAA to investigate the causes of cell death. METHODS： The cell viability of TAA-induced clone 9 cells was determined using MTT assay. Total cellular GSH in TAA-induced clone 9 cells was measured using a slight modification of the Tietze assay. The activity of caspase 3 in TAA-induced clone 9 cells was monitored by the cleavage of DEVD-p-nitroanaline. TUNEL assay and flow cytometry were applied for the determination of DNA fragmentation and the proportion of apoptosis in TAA- induced clone 9 cells, respectively. The alterations of caspase 3, Bad, Bax and Phospho-P53 contents in TAA- induced clone 9 cells were measured by Western blot. RESULTS： The experimental data indicated that TAA caused rat hepatic epithelial cell line clone 9 cell death in a dose-and time-dependent manner; 60% of the cells died （MTT assay） within 24 h after 100 rag/1 TAA was applied. Apoptotic cell percentage （TUNE1 assay） and caspase 3 activities were highest after 100 rag/1 TAA was added for 8 h. The release of GSH and the elevation in caspase content after TAA treatment resulted in clone 9 cell apoptosis via oxidative stress and a caspasedependent mechanism. The phospho-p53, Bax and Bad protein expressions in clone 9 cells were increased after TAA treatment. CONCLUSION： These results reveal that TAA activates p53, increases caspase 3, Bax and Bad protein contents, perhaps causing the release of cytochrome c from mitochondria and the disintegration of membranes, leading to apoptosis of cells.

关键词： Thioacetamide, Phospho-p53 Caspase 3,Apoptosis Bax, Bad

Development of a rapid test kit for SARS-CoV-2:an example of product design

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Bio-Design and Manufacturing 2020年第2期3卷 83-86页

作者： Zhanfeng Cui Hong Chang Hui Wang boon Lim chia-Chen Hsu Yejiong Yu Huidong Jia Yun Wang Yida Zeng Mengmeng Ji Weizhi Liu Catriona Inverarity Wei E.Huang Oxford Suzhou Centre for Advanced Research(OSCAR) University of OxfordSuzhouJiangsuPeople's Republic of China Department of Engineering Science University of OxfordParks RoadOxford OX13PJUK Department of Engineering Science Institute of Biomedical EngineeringUniversity of OxfordOxford OX37DQUK

We present an example of applying'need-driven'product design principle to the development of a rapid test kit to detect SARS-COV-2(COVID-19).The tests are intended for use in the field and,longer term,for home *** detect whether a subject is currently infected with the virus and is *** urgent need for large numbers of tests in field setting imposes constraints such as short test time and lack of access to specialist equipment,laboratories and skilled technicians to perform the test and interpret *** meet these needs,an antigen test based on RT-LAMP with colorimetric readout was *** use of swab sample with no RNA extraction was *** extensive experimental study(reported elsewhere),a rapid test kit has been fabricated to satisfy all design criteria.

关键词： COVID-19 Antigen detection Rapid test Product design SAR-Cov-2 RT-LAMP

The Ode of The Eagle Spirit

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福建师范大学学报(哲学社会科学版) 1959年第2期 113-114页

作者： Tieng Dzien weng Ping-chia

A hero coming from the land of the brave,He grew up on the sea-coast,This our hero, Chou Chuen Fu,Flying an eagle spirit;Following the squadron on its returning flight,Suddenly heard the motors in the *** appeared in the cloud,Their golden bodies flashing in the *** at once, Chou Chuen Fu,Discovered the enemy planes in the *** the sake of covering the squadron,He disregarded his own life and put about his plane,Though he saw the apparent dangor,He yet resolved to save others by losing himself.O eagle spirit, the eagle spirit of our Motherland,

关键词： The Ode of The Eagle Spirit

Observer Design —— A Survey

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International Journal of Automation and computing 2015年第1期12卷 50-61页

作者： chia-Chi Tsui Staten Island USA

This paper surveys the results of observer design for linear time-invariant(L-T-I) deterministic irreducible open-loop systems(OLS), the most basic type of OLS. An observer estimates Kxx(t) signal where K is a constant and x(t) is the state vector of the OLS. Thus, an observer can be used as a feedback controller that implements state feedback control(SFC) or Kxx(t)-control,and observer design is therefore utterly important in all feedback control designs of state space theory. In this survey, the observer design results are divided into three categories and for three respective main purposes. The first category of observers estimate signal Kxx(t) only with a given K, and this survey has four conclusions: 1) Function observer that estimates Kxx(t) directly is more general than state observer that estimates x(t), and may be designed with order lower than that of state observer, and the additional design objective is to minimize observer order; 2) The function observer design problem has already been simplified to the solving of a single set of linear equations only while seeking the lowest possible number of rows of the solution matrix, and an apparently most effective and general algorithm of solving such a problem can guarantee unified upper and lower bounds of the observer order; 3) Because such a single set of linear equations is the simplest possible theoretical formulation of the design problem and such theoretical observer order bounds are the lowest possible, and because the general, simple, and explicit theoretical formula for the function observer order itself do not exist, the theoretical part of this design problem is solved; 4) Because the function observer order is generically near its upper bound, further improvement on the computational design algorithm so that the corresponding observer order can be further reduced,is generically not worthwhile. The second category of observers further realize the loop transfer function and robustness properties of t

关键词： Low order function observer robust output feedback observer synthesized design principle faults and time-delays

Thickness bound for nonlocal wide-field-of-view metalenses

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Light(Science & Applications) 2022年第12期11卷 3018-3027页

作者： Shiyu Li chia Wei Hsu Ming Hsieh Department of Electrical and Computer Engineering University of Southern CaliforniaLos AngelesCA 90089USA

Metalenses—flat lenses made with optical metasurfaces—promise to enable thinner,cheaper,and better imaging *** a sufficient angular field of view(FOV)is crucial toward that goal and requires a tailored incidentangle-dependent ***,we show that there is an intrinsic trade-off between achieving a desired broad-angle response and reducing the thickness of the *** the memory effect in disordered media,this thickness bound originates from the Fourier transform duality between space and *** can write down the transmission matrix describing the desired angle-dependent response,convert it to the spatial basis where its degree of nonlocality can be quantified through a lateral spreading,and determine the minimal device thickness based on such a required lateral *** approach is *** applied to wide-FOV lenses,it predicts the minimal thickness as a function of the FOV,lens diameter,and numerical *** bound is tight,as some inverse-designed multi-layer metasurfaces can approach the minimal thickness we *** work offers guidance for the design of nonlocal metasurfaces,proposes a new framework for establishing bounds,and reveals the relation between angular diversity and spatial footprint in multi-channel systems.

关键词： desired angular field

On Variance and Covariance for Bounded Linear Operators

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Acta Mathematica Sinica,English Series 2001年第4期17卷 657-668页

作者： chia Shiang LIN Department of Mathematics Bishop's University Lennoxville P. Q. J1M 1Z7. Canada

In this paper we initiate a study of covariance and variance for two operators on a Hilbert space. proving that the c-v (covariance-variance) inequality holds, which is equivalent to the Cauchy- Schwarz inequality. As for applications of the c-v inequality we provc uniformly the Bernstein-type inequalities and equalities. and show the generalized Heinz-Kato-Furuta-type inequalities and equalities. from which a generalization and sharpening of Reid’s inequlality is obtained. We show that every operator can be expressed as a p-hyponormal-type, and a hyponormal-type operator. Finally, some new characterizations of the Furuta inequality are given.

关键词： Covariance-variance inequality Bernstein inequality Reid’s inequality Furuta inequality Lowner-Heinz formula

The fabrication and application of Ni-DNA nanowire-based nanoelectronic devices

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Nano Research 2019年第6期12卷 1293-1300页

作者： Pang-chia Chang chia-Yu Chang Wen-Bin Jian Chiun-Jye Yuan Yu-Chang Chen chia-Ching Chang Department of Electrophysics "National Chiao Tung University"Hsinchu 30010TaiwanChina Department of Biological Science and Technology "National Chiao Tung University"Hsinchu 30068TaiwanChina Center for Intelligent Drug Systems and Smart Bio-devices(IDS^2B) "National Chiao Tung University"Hsinchu 30068TaiwanChina Institute of Physics "Academia Sinica"Taipei 11529TaiwanChina

DNA is a self-assembled,double stranded natural molecule that can chelate and align nickel ions between its base *** fabrication of a DNA-guided nickel ion chain (Ni-DNA) device was successful,as indicated by the conducting currents exhibiting a Ni ion redox reaction-driven negative differential resistance effect,a property unique to mem-elements (1).The redox state of nickel ions in the Ni-DNA device is programmable by applying an external bias with different polarities and writing times (2).The multiple states of Ni-DNA-based memristive and memcapacitive systems were characterized (3).As such,the development of Ni-DNA nanowire device-based circuits in the near future is proposed.

关键词： DNA-guided nickel ion chain (Ni-DNA) negative differential resistance (NDR) memristive systemmem capacitive system nanowire