检索结果-南通市图书馆

Genomic Deletion Induced by To12 Transposon Excision in Zebrafish

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genomic Deletion Induced by To12 Transposon Excision in Zebr...

High glucose reduces Nrf2-dependent cRAGE release and enhances inflammasome-dependent IL-1βproduction in monocytes:the modulatory effects of EGCG

“基因、进化与生理功能多样性”海内外学术研讨会暨中国生理学会第七届比较生理学学术会议

作者： chi-yen Tam Key Laboratory of Cell Proliferation and Differentiation Center of Developmental Biology and GeneticsCollege of Life SciencesPeking UniversityMinistry of EducationBeijing

Imprecise excision of P element is widely used in Drosophila to disrupt nearby ***,this is the only transposon reported for such an application so far,while no similar approach has been documented in ***2 transposon has been successfully introduced in zebrafish(Danio rerio) and lots of transgenic fish have been created via Tol2 *** this report,we tested the excision efficiency of this transposon using a transgenic fish line Et(lmo2:eGFP),in which Tol2 transposon had inserted at 243bp upstream of *** injecting Tol2 transposase mRNA to homozygous Et(lmo2:eGFP) embryos,we obtained mosaic founders and crossed them with wild type *** GFP-negative embryos and embryos with GFP patterns distinct from the original one were observed,indicating that Tol2 had jumped out from the original *** excision efficiency of Tol2 transposon from zebrafish genome was about 40%by this *** results of footprints showed that imprecise excisions with small deletions or additions happened with some of Tol2 jump out *** identified a 1kb genomic deletion flanking Tol2 5' terminal out of 59 founder *** our knowledge,this is the first report of identification of large genomic deletions induced by imprecise transposon excision in verte- *** result suggests that Tol2 transposon "jump out" could be a new approach for mutagenesis in zebrafish.

关键词： Zebrafish Tol2 Transposon Deletion

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2024年第3期13卷 1531-1542页

作者： chi-Hao Wu Yin-Hsuan Chang chin-Lin Hsu Sheng-Yi Chen Gow-chin yen Graduate Programs of Nutrition Science School of Life ScienceTaiwan Normal UniversityTaipei 106209TaiwanChina Department of Food Science and Biotechnology Chung Hsing UniversityTaichung 40227TaiwanChina School of Nutrition Chung Shan Medical UniversityTaichung 40201TaiwanChina

Soluble receptor for advanced glycation end products(sRAGE)acts as a decoy sequestering of RAGE ligands,thus preventing the activation of the ligand-RAGE axis linking human ***,the molecular mechanisms underlying sRAGE remain *** this study,THP-1 monocytes were cultured in normal glucose(NG,5.5 mmol/L)and high glucose(HG,15 mmol/L)to investigate the effects of diabetesrelevant glucose concentrations on sRAGE and interleukin-1β(IL-1β)*** modulatory effects of epigallocatechin gallate(EGCG)in response to HG challenge were also *** enhanced intracellular reactive oxygen species(ROS)generation and RAGE *** secretion of sRAGE,including esRAGE and cRAGE,was reduced under HG conditions,together with the downregulation of a disintegrin and metallopeptidase 10(ADAM10)and nuclear factor erythroid 2-related factor 2(Nrf2)nuclear ***,the HG effects were counteracted by siRAGE and exacerbated by *** immunoprecipitation results showed that Nrf2 binding to the ADAM10 promoter and HG interfered with this *** data reinforce the notion that RAGE and Nrf2 might be sRAGE-regulating *** HG conditions,the treatment of EGCG reduced ROS generation and RAGE ***-stimulated cRAGE release was likely caused by the upregulation of the Nrf2-ADAM10 *** inhibited HG-mediated NLRP3 inflammasome activation at least partly by stimulating sRAGE,thereby reducing IL-1βrelease.

关键词： Epigallocatechin gallate(EGCG) Inflammasome Nuclear factor erythroid 2-related factor 2(Nrf2) Receptor for advanced glycation end products(RAGE) Soluble RAGE(sRAGE)

木原均,.洛夫与小麦族(禾本科)的现代遗传学属的概念(英文)

在线全文

学校读者我要写书评

暂无评论

植物分类学报 2005年第1期43卷 82-93页

作者：颜济杨俊良颜旸四川农业大学小麦研究所 Department of Biology and Microbiology South Dakota State University Brookings SD 57006 USA

分类学是认识生物体的一种工具,对生物体间系统关系的理解,种质资源利用的指南,也是一种交流用的普通语言。因此,分类处理需要反映这些关系的近期认识。在自然界,生物体只有两个绝对的单位:个体与种。一个种是一群个体被不可缺少的生殖... 详细信息

分类学是认识生物体的一种工具,对生物体间系统关系的理解,种质资源利用的指南,也是一种交流用的普通语言。因此,分类处理需要反映这些关系的近期认识。在自然界,生物体只有两个绝对的单位:个体与种。一个种是一群个体被不可缺少的生殖关系相互联系成为的一个绝对单位。生殖隔离是种与种间的基本界限,同时也是生物演化过程中形成独立基因库(gene pools)的惟一因素。既然在种以上的分类群没有绝对界线,在种以上的任一分类处理都不可能避免人为性。虽然如此,仍然必须作出某些分类适应它们的描述、利用与(或)研究。这篇文章对小麦族分类群间生物系统关系的划分是基于遗传学的研究。我们分类处理的原则是:(1)反映这些种系统演化现今的理解;(2)便于种质资源的利用;(3)避免与传统处理有不必要的剧烈改变。

关键词：禾本科小麦族现代遗传学分类生殖隔离独立基因库种质资源生物系统学染色体组系统演化

DR. A. PEN-TUNG SAH, a brief biographical note

在线全文

学校读者我要写书评

暂无评论

中国物理学报 1950年第1期 305-308页

作者： chi-SUN yen Department of Physics, National Tsing Hua University

On January 31st,1949,***-Tung Sah died in the Universityof California Hospital in San *** hearing the news,chineseeducational and scientific workers and others who had attended hislectures and/or read his writings were stricken with deep *** was the loss of an important research worker in the fields of physics and electrical engineering in china,that of an able and conscientious

关键词： SAH PEN

ON101 Cream Increases the Wound Healing Rate in Diabetic Patients with Uremia—Cases Report

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Case Reports in Clinical Medicine 2024年第5期13卷 178-186页

作者： Yu-Hsiu yen chi-Ming Pu Division of Plastic Surgery Department of Surgery Cathay General Hospital Taipei City School of Medicine College of Medicine Fu Jen Catholic University New Taipei School of Medicine College of Life Science National Tsing Hua University Hsinchu

Diabetic patients who underwent long-term dialysis may increase the prevalence of foot ulceration. In addition, diabetic foot ulcer (DFUs) patients with end-stage renal disease (ESRD) do not heal well, and the amputation rate is 6.5 - 10 times higher compared to the non-nephropathic diabetic population. Thus, a suitable therapeutic agent was needed. ON101 is a topical cream that promotes diabetic wound healing through a unique macrophage-regulating ability. In this case series, we included 5 diabetes patients (mean age 54.6 ± 8.7 years, 4 mal) with ESRD (mean eGFR 7.4 ± 3.35 mL/min/1.73m2) and had experienced dialysis for at least 4.5 years. These patients also have UT (University of Texas) grade 2A DFUs that have existed for at least 1.5 months (mean ulcer duration 8.3 ± 8.97 months). These subjects were applied ON101 twice daily for up to 20 weeks, and wound size was recorded during treatment. Among these subjects, three ulcers (patient No. 1, 2, and 3) completely healed within 10 weeks upon ON101 application, and one ulcer was 99% reduced at 20th weeks (patient No. 4). Only one ulcer didn’t show an obvious response that may due to poor compliance in wound care and glucose control. In summary, the overall healing rate was 60%, suggesting ON101 performed equivalence healing efficacy in dialysis patients compared with those who did not have dialysis.

关键词： Diabetic Foot Ulcer (DFU) Uremia Dialysis Wound Healing

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

如何避免贫乏性结果

逻辑学研究 2018年第4期11卷 56-82页

作者：刘吉宴京都大学大学院文学研究科哲学研究室阳明大学心智哲学研究所

亚当斯论题往往被解读为:在P(A)> 0的条件下,直陈条件句"如果A,B"的主观概率等同于条件概率P (B|A)。许多人指出这样的解读会遭受贫乏性结果的挑战,因此建议我们放弃亚当斯论题;然而,本论文论证刘吉宴(2014)对亚当斯论题的解读可以避免... 详细信息

亚当斯论题往往被解读为:在P(A)> 0的条件下,直陈条件句"如果A,B"的主观概率等同于条件概率P (B|A)。许多人指出这样的解读会遭受贫乏性结果的挑战,因此建议我们放弃亚当斯论题;然而,本论文论证刘吉宴(2014)对亚当斯论题的解读可以避免贫乏性结果的攻击。刘吉宴(2014)区分了直陈条件句为真的概率与可断说性,并对亚当斯论题提出以下的解读:在P(A)> 0的条件下,简单直陈条件句"如果A,B"的可断说性等同于条件概率P(B|A),同时在三值语意学的观点下,对此提出一个形式上的证明。刘吉宴(2014)虽然说明了这个结果如何避免刘易士的第一个贫乏性结果,但没有详细讨论这如何避免其它的贫乏性结果。本论文将进一步扩展该文对亚当斯论题的想法,并论证其它文献中的贫乏性结果,也可以从这个扩展后的想法获得恰当的解决。

关键词：直陈条件句亚当斯论题贫乏性结果三值语意论可断说性

在线全文

学校读者我要写书评

暂无评论

条件概率、连言概率与缺漏真值表

逻辑学研究 2017年第1期10卷 54-78页

作者：刘吉宴京都大学大学院文学研究科哲学研究室

心理学家很早就开始研究指示条件句的语意论,这近十几年来更试图从指示条件句的概率着手,再回头来看看什么样的语意论最能够解释实验数据。从这些实验中出现了三个显著的结果:第一、大多数的人认为指示条件句的概率等于条件概率;第二、... 详细信息

心理学家很早就开始研究指示条件句的语意论,这近十几年来更试图从指示条件句的概率着手,再回头来看看什么样的语意论最能够解释实验数据。从这些实验中出现了三个显著的结果:第一、大多数的人认为指示条件句的概率等于条件概率;第二、为数不少的人认为指示条件句的概率等于其前件与后件为真的连言概率;第三、大多数的人对指示条件句的真假值看法采取缺漏真值表。前两个结果对心理学家造成极大的难题,为何会出现这样的结果?有没有一个统一的语意论能去解释它?心理学家们几乎全在辩护条件概率者的看法才是正确的,并试图提出条件概率者背后的心理机制,并在此基础上试图解释为何连言概率者会犯错。然而,笔者会在本文论证这个方向是错误的;本文主张我们应该从另一个方向来解释条件概率和连言概率说法之间的关联。笔者认为解套的关键在于缺漏真值表,笔者建议从这个心理学很早就发现的重要结果出发,试图发展出一个三值语意论,比较有机会帮助心理学家解决这个难题。笔者给出两个理由来捍卫这个立场,首先,贫乏性结果已指出条件概率不可能是指示条件句为真的概率;再者,笔者会说明刘吉宴提出的三值语意论如何帮助心理学家们解决这个问题。(刘吉宴,2014)最后笔者得出结论,连言概率说法比条件概率说法更为可信。

关键词：条件句概率缺漏真值表贫乏性结果

Effect of osmotic shock on the redox system in plasma membrane of Dunaliella salina

在线全文

学校读者我要写书评

暂无评论

Cell Research 1996年第1期6卷 31-38页

作者： CHEN SI XUE chi chiONG yen XIN ZHI JIAO (Institute of Plant Physiology, chinese Academy of Sciences, Shanghai 200032, china.)(Biology Department, East china Normal University,Shanghai 200062, china.) Institute of Plant Physiology Chinese Academy of Sciences Shanghai China Biology Department East China Normal University Shanghai China

The unicellular halotolerant alga Dunaliella salina had the ability to oxidize NADH and reduce Fe(CN)63-. The redox reactions were to some extent stimulated by slight hyperosmotic shock (2.0 mol/L → 2.6 mol/L NaCl), butmarkably inhibited by abrupt hyperosmotic shock (2.0mol/L → 3.5 mol/L NaCl) and hypoosmotic shock (2.0mol/L → 1.0 mol/L NaCl; 2.0 mol/L→0.67 mol/L NaCl).With the adaptation of algal cells to osmotic shock by accumulating or degrading intracellular glycerol, the plasmalemma redox activities were also restored. The O2 uptake stimulated by NADH could be promoted by FA and SHAM. Hypoosmotic shock increases the basal respiration rate of alga cells, but weakened the stimulating effects of NADH, FA and SHAM on O2 uptake. On the other hand, hyperosmotic shock reduced the basal respiration rate, but relatively enhanced the above effects of NADH, FA and SHAM. H+ extrusion of alga cells was inhibited by NADH and stimulated by Fe(CN)63- Vanadate and DES could inhibit H+ efflux, but had little effect in the presence of NADH and Fe(CN)63-. Both hyperand hypoosmotic shock stimulated H+ extrusion. This effect could be totally inhibited by vanadate and DES, but almost unaffected by 8-hydroxyquinoline. It was suggested that H+-ATPase probably played a more important role in H+ extrusion and osmoregulation under the conditions of osmotic shock.

关键词： Osmotic shock Dunaliella salina plasmalemma redox system

A Network Signal Timing Design Bilevel Optimization Model with Traveler Trip-Chain Route Choice Behavior Consideration

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Traffic and Transportation Engineering 2017年第4期5卷 203-216页

作者： Chung-Yung Wang Kuo-chi yen Shou-Ren Hu chih-Peng Chu Ya-Tin Jhuang Department of Logistics Management National Defense University Taipei City 112 Taiwan R.O.C. Department of Transportation and Communication Management Science National Cheng Kung University Tainan City 701Taiwan R. O. C. " Department of Business Administration National Dong Hwa University Hualien City 9 74 Taiwan R.O.C.

This study proposed a bilevel optimization model for the network signal timing design problem by considering the linkflows reflected by the trip-chain route choice behaviors of road users. The bilevel programming model is formulated based on theinteractions between signal timing control and trip-chain behavior, and a solution algorithm by combining variational inequalitysensitivity analysis, the generalized inverse matrix method, and a gradient projection approach revised for trip-chain user equilibriumis developed for the transportation design problem. The performance of the developed model framework was verified throughnumerical analysis under different test scenarios.

关键词： Signal timing design, bilevel programming model, trip-chain user equilibrium.