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A bHLH transcription factor,CsSPT,regulates high-temperature resistance in cucumber

Horticultural Plant Journal 2024年第2期10卷 503-514页

作者： Yonggui Liang chenyu yang Fangyan Ming Bingwei Yu Zhihua Cheng Yixi Wang Zhengkun Qiu Xiaolan Zhang Bihao Cao Shuangshuang Yan Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsGuangdong Vegetable Engineering and Technology Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhouGuangdong 510642China State Key Laboratories of Agrobiotechnology Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable CropsMOE Joint Laboratory for International Cooperation in Crop Molecular BreedingChina Agricultural UniversityBeijing 100193China

High-temperature stress threatens the growth and yield of crops. Basic helix-loop-helix(bHLH) transcription factors(TFs) have been shown to play important roles in regulating high-temperature resistance in plants. However, the bHLH TFs responsible for high-temperature tolerance in cucumbers have not been identified. We used transcriptome profiling to screen the high temperature-responsive candidate bHLH TFs in cucumber. Here, we found that the expression of 75 CsbHLH genes was altered under high-temperature stress. The expression of the CsSPT gene was induced by high temperatures in TT(Thermotolerant) cucumber plants. However, the Csspt mutant plants obtained by the CRISPR-Cas9 system showed severe thermosensitive symptoms, including wilted leaves with brown margins and reduced root density and cell *** Csspt mutant plants also exhibited elevated H_(2)O_(2) levels and down-regulated photosystem-related genes under normal ***, there were high relative electrolytic leakage(REC), malondialdehyde(MDA), glutathione(GSH), and superoxide radical(O_(2)^(·-)) levels in the Csspt mutant plants, with decreased Proline content after the high-temperature treatment. Transcriptome analysis showed that the photosystem and chloroplast activities in Csspt mutant plants were extremely disrupted by the high-temperature stress compared with wildtype(WT) plants. Moreover, the plant hormone signal transduction, as well as MAPK and calcium signaling pathways were activated in Csspt mutant plants under high-temperature stress. The HSF and HSP family genes shared the same upregulated expression patterns in Csspt and WT plants under high-temperature conditions. However, most bHLH, NAC, and bZIP family genes were significantly down-regulated by heat in Csspt mutant plants. Thus, these results demonstrated that CsSPT regulated the high-temperature response by recruiting photosynthesis components, signaling pathway molecules, and transcription factors. Our results pro

关键词： Cucumber bHLH CsSPT Photosynthesis High temperature

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4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI

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Chinese Chemical Letters 2024年第6期35卷 486-490页

作者： Hualei Xu Manman Han Haiqiang Liu Liang Qin Lulu Chen Hao Hu Ran Wu chenyu yang Hua Guo Jinrong Li Jinxiang Fu Qichen Hao Yijun Zhou Jinchao Feng Xiaodong Wang Key Laboratory of Mass Spectrometry Imaging and Metabolomics(Minzu University of China) State Ethnic Affairs CommissionBeijing 100081China College of Life and Environmental Sciences Centre for Imaging&Systems BiologyMinzu University of ChinaBeijing 100081China

Low-molecular-weight(LMW)compounds are ubiquitous in living organisms and play essential roles in biological *** direct analysis of LMW compounds in biological tissues by matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI)could provide a more comprehensive understanding of their essential ***,we evaluated 4-nitrocatechol(4-NC)as a novel positive-ion matrix for enhancing in situ detection and imaging of LMW compounds from the rat liver,brain,and germinating Chinese-yew seed by *** results showed that the 4-NC possessed remarkable features,including strong ultraviolet absorption,uniform matrix crystal,excellent chemical stability,and fewer matrix-related background *** use of 4-NC led to the successful detection of 232,218,and193 LMW compounds from the three abovementioned tissue sections,***,the use of 4-NC improved the imaging quality of LMW compounds in tissue sections through MALDI-MSI and has the potential as a matrix for MALDI tissue imaging of LMW compounds.

关键词： MALDI-MSI 4-Nitrocatechol LMW compounds In situ detection Tissue imaging

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Investigations on affinity law under gas–liquid conditions in multistage radial and mixed-flow multiphase pumps

International Journal of Fluid Engineering

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International Journal of Fluid Engineering 2024年第1期 56-70页

作者： Liang Chang chenyu yang Xiaobin Su Xiaoyu Dai Qiang Xu Liejin Guo State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Affinity laws have been widely used in pump design and simulation under high-temperature and corrosive conditions. By applying such laws,it is possible to shorten development cycles and reduce test costs. However, current applications of affinity laws are still limited to liquid conditions. In this paper, expressions for affinity laws and their applicability are investigated for multistage radial and mixed-flow multiphase pumps under gas–liquid conditions. A high-pressure（30 MPa） gas–liquid experimental platform is constructed, and three-stage and 25-stage radial pumps and a 15-stage mixed-flow pump are investigated, with specific speeds of 107 and 216. With gas compressibility taken into account, the gas–liquid two-phase flow rate, head, and power, and the corresponding dimensionless hydraulic coefficients, are defined for multiphase pumps. The deterioration of gas–liquid pressurization performance is found to be divided into three processes with different dynamic mechanisms, corresponding to three flow patterns. The inlet gas volume fraction of pump is used to judge dynamic similarity. At the same inlet gas volume fractions λ1= λ2, when the gas–liquid flows in two pumps have the same flow pattern, dynamic similarity will be satisfied. The affinity law that is established shows good applicability to the three-stage radial multiphase pump, with goodness of fit R2larger than 0.9 for the two-phase Ψm–Φmand Πm–Φmperformance curves. Finally, experimental results indicate that the affinity law also has good applicability to multiphase pumps with different stage numbers and blade structures under gas–liquid conditions.

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考虑非达西渗流的泥浆离心脱水分析

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哈尔滨工业大学学报 2024年第7期56卷 85-93页

作者：刘忠玉刘朝凡杨宸宇汪良强郑州大学水利与土木工程学院郑州450001 郑州三和水工机械有限公司郑州450120

为深入探究离心脱水技术在处理主要由黏性土组成的建筑废弃泥浆时的内在机理及其影响因素,利用分段线性化方法,充分考虑黏性土中渗流的非达西特性以及土体变形的非线性特征,建立在离心作用下能够考虑其非达西渗流特性的泥浆脱水分析模... 详细信息

为深入探究离心脱水技术在处理主要由黏性土组成的建筑废弃泥浆时的内在机理及其影响因素,利用分段线性化方法,充分考虑黏性土中渗流的非达西特性以及土体变形的非线性特征,建立在离心作用下能够考虑其非达西渗流特性的泥浆脱水分析模型。通过与已有研究成果及室内试验结果的对比,证明了该模型的有效性。在此基础上,分析了模型参数对脱水过程的影响。研究结果表明:脱水速度和最终土水界面位移量随着电机转速的增大而增大;增加泥浆初始厚度会增大最终土水界面位移量,但会降低初期的土水界面移动速度;压缩指数在初期对土水界面位移的影响并不明显,但其增大会导致最终土水界面位移量的增加;非达西渗流参数的增大会导致土水界面位移的减少,但通过提升电机转速,可以有效地降低非达西渗流对脱水效果的不利影响。研究成果可为离心脱水技术在建筑废弃泥浆处理中的进一步优化与应用提供借鉴和参考。

关键词：泥浆离心脱水非达西渗流土水界面位移界面移动速度

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离心泵内气液两相流动的数值模拟与实验研究

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工程热物理学报 2024年第8期45卷 2396-2402页

作者：苏筱斌徐强杨晨宇戴晓宇郭烈锦西安交通大学动力工程多相流国家重点实验室西安710049

分叶式叶轮由于其叶片的分离和偏移能够很好地处理气液流动而被广泛应用在石油工业中。本文采用叶轮可视化及数值模拟相结合的方法研究了分叶式叶轮内的气液流动特性。在该叶轮中,气团会率先出现在内叶片入口及外叶片的吸力面。气团在... 详细信息

分叶式叶轮由于其叶片的分离和偏移能够很好地处理气液流动而被广泛应用在石油工业中。本文采用叶轮可视化及数值模拟相结合的方法研究了分叶式叶轮内的气液流动特性。在该叶轮中,气团会率先出现在内叶片入口及外叶片的吸力面。气团在延伸至叶片分离和偏移的位置时,受到来自相邻流道流体的冲击。采用欧拉–欧拉模型时,数值模拟得到的叶轮内气液流动与可视化得到的结果基本一致。在叶轮流道中,被气团占据的区域失去了增压能力,而气团未占据的区域仍保留一定的增压性能。

关键词：离心泵叶轮可视化数值模拟气液流动

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Multidisciplinary and multiscale nanoscience research roadmap based on large scientific facilities

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Science China Chemistry 2024年第8期67卷 2497-2523页

作者： Yuan Zeng Shuhan Zhang Yuecong Guo Yalin Cong Xu Ding Peihua Li Yunxiang Lin Wenzhi Ren Hui Su Weigang Sun Liuxin Xu Guikai Zhang Shihua Chen Yu Chen Weiren Cheng Shengqi Chu Yong Guan Jinru Han Jie Lin Hengjie Liu Zheyi Liu Pan Luo Fanchun Meng Sicong Qiao Zongyin Song Ying Wang Zhao Wu chenyu yang Meng yang Shirui yang Zi Yin Zhibin Yin Pengjun Zhang Hongyu Zhang Pingping Zheng Jia Zhou Wanlin Zhou Pengfei An He Cheng Chunhai Fan Xingjiu Huang Yong Lei Lina Li Mu Li Qinghua Liu Shuming Peng Li Song Zhihu Sun yangchao Tian Fangjun Wang Lihua Wang Liming Wang Shiqiang Wei Aiguo Wu Chunlei Xiao Xueming yang Panchao Yin Jing Zhang Mingxin Zhang Yaling Wang Chunying Chen CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety New Cornerstone Science LaboratoryNational Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China Institute of Solid State Physics Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology Zhejiang International Cooperation Base of Biomedical Materials Technology and ApplicationCAS Key Laboratory of Magnetic Materials and DevicesNingbo Cixi Institute of Biomedical EngineeringZhejiang Engineering Research Center for Biomedical MaterialsNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China Advanced Energy Science and Technology Guangdong Laboratory Huizhou 516000China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410081China State Key Laboratory of Luminescent Materials and Devices South China Advanced Institute for Soft Matter Science and TechnologySouth China University of TechnologyGuangzhou 510640China Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China CAS Key Laboratory of Separation Sciences for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Institute of Advanced Science Facilities Shenzhen 518107China State Key Laboratory of Coal Conversion Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China State Key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Acade

With the advancement of modern science and technology, large scientific facilities are increasingly oriented toward demand and application, and can be used for basic research as well as serving multiple disciplines. Developing large scientific facilities and related analytical technologies enhances understanding of large scientific facilities and popularizes their application in research across multiple disciplines. The combination of light or neutron sources from large scientific facilities and advanced analytical technologies can be achieved for materials structure information, dynamics study of chemical reactions, high dissociation of biomolecules, 3D visualization of energy materials or biological samples, etc. We first introduce the progress of domestic large scientific facilities of synchrotron radiation(SR) and free electron lasers(FELs) with different wavelengths and neutron *** further discuss the comparison between Chinese and typical foreign facilities in X-ray radiation from X-ray tubes, synchrotrons, X-ray FELs, and neutron sources based on physical parameters of light and neutron sources. In addition, we focus on the technological progress and perspectives combined with advanced X-ray radiation and neutron sources of large scientific facilities in China, especially in the nanoscience fields of energy catalysis and biological science. We hope that this roadmap will provide references on technology and methods to experimental users, as well as prospects for future development of technologies based on large research infrastructure facilities. Comprehensive studies and guidelines for basic research to practical application in various disciplines can be made with the assistance of large scientific facilities.

关键词： large scientific facilities synchrotron radiation FEL light sources neutron sources spectroscopic analysis methods X-ray imaging techniques structural information chemical dynamics

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基于数字全息的云中冰晶微物理参数观测方法

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光学学报 2024年第6期44卷 188-197页

作者：杨晨遇王骏张川周浩杨军胜岳治国梁谷刘晶晶华灯鑫西安理工大学机械与精密仪器工程学院陕西西安710048 陕西省人工影响天气中心陕西西安710016

冰晶是云滴谱的重要组成部分,其对全球辐射收支平衡、全球气候变化、水文循环、人工影响天气作业等具有重要的影响。目前2~100μm的冰晶与液滴混合相态难以区分,且存在难以提供冰晶微物理参数的瓶颈问题。针对这两个问题,本文基于数字... 详细信息

冰晶是云滴谱的重要组成部分,其对全球辐射收支平衡、全球气候变化、水文循环、人工影响天气作业等具有重要的影响。目前2~100μm的冰晶与液滴混合相态难以区分,且存在难以提供冰晶微物理参数的瓶颈问题。针对这两个问题,本文基于数字全息理论,利用全域数字图像融合方法、局部亮度梯度方差法和旋转卡壳法,结合固液相粒子圆度概念,实现了云中液滴和冰晶的混合相态识别,在特定圆度阈值下,液滴和冰晶的识别率大于93%;再结合光学图像识别技术,获得冰晶粒子的面积、周长、凸包和最小外接矩形数据;最终利用上述数据获得了冰晶微物理参数。通过在低温云室中的观测实验,获取了板状、枝状和六角冰晶的微物理参数,该方法解决了冰晶观测中的瓶颈问题。此外,通过冰晶采样间隔时间和不同时刻的质心三维坐标和等效直径,还可获得冰晶粒子的三维运动速度与轨迹。该方法对提升数值天气预报精确度,以及人工影响天气作业具有重大意义。

关键词：数字全息粒子相态识别冰晶粒子速度

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Memristor bridge-based low pass filter for image processing

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Journal of Systems Engineering and Electronics 2019年第3期30卷 448-455页

作者： YU Yongbin yang Nijing yang chenyu NYIMA Tashi School of Information and Software Engineering University of Electronic Science and Technology of China Chengdu 610054 China School of Information Science and Technology Tibet University Lhasa 850012 China

This paper highlights the memristor bridge-based lowpass filter (LPF) and improved image processing algorithms along with a novel adaptive Gaussian filter for denoising image and a new Gaussian pyramid for scale invariant feature transform (SIFT). First, a novel kind of LPF based on the memristor bridge is designed, whose cut-off frequency and other traits are demonstrated to change with different time and memristance. In light of the changeable parameter of the memristor bridge-based LPF, a new adaptive Gaussian filter and an improved SIFT algorithm are presented. Finally, experiment results show that the peak signalto- noise ratio (PSNR) of our denoising is bettered more than 2.77 dB compared to the corresponding of the traditional Gaussian filter, and our improved SIFT performances including the number of matched feature points and the percent of correct matches are higher than the traditional SIFT, which verifies feasibility and effectiveness of our algorithm.

关键词： memristor bridge low-pass filter (LPF) adaptive Gaussian filter image denoising Gaussian pyramid

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Au^(δ-)-O_(v)-Ti^(3+):Active site of MO_(x)-Au/TiO_(2) catalysts for the aerobic oxidation of 5-hydroxymethylfurfural

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Green Energy & Environment 2023年第3期8卷 785-797页

作者： Weiyao yang Mengchen Fu chenyu yang Yiwen Zhang Chun Shen Beijing Key Laboratory of Bioprocess College of Life Science and TechnologyBeijing University of Chemical TechnologyNo.15 of North Three-Ring East RoadChaoyang DistrictBeijing100029China The State Key Laboratory of Chemical Engineering Department of Chemical EngineeringTsinghua UniversityBeijing100084China

Despite wide applications of noble metal-based catalysts in 5-hydroxymethylfurfural(HMF)oxidation,promoting the catalytic performance at low loading amounts still remains a significant ***,a series of metal oxide modified MO_(x)-Au/TiO_(2)(M=Fe,Co,Ni)catalysts with low Au loading amount of 0.5 wt%were *** of transition metal oxides promotes electron transfer and generation of the Au^(δ-)-O_(v)-Ti^(3+)interface.A combination study reveals that the dual-active site(Au^(δ-)-O_(v)-Ti^(3+))governs the catalytic performance of the ratedetermining step,namely hydroxyl group ***^(δ-) site facilitates chemisorption and activation of O_(2) *** the same time,O_(v)-Ti^(3+) site acts as the role of“killing two birds with one stone”:enhancing adsorption of both reactants,accelerating the activation and dissociation of H_(2)O,and facilitating activation of the adsorbed O_(2).Besides,superoxide radicals instead of base is the active oxygen species during the rate-determining *** this basis,a FDCA yield of 71.2% was achieved under base-free conditions,complying with the“green chemistry”*** work provides a new strategy for the transition metal oxides modification of Au-based catalysts,which would be constructive for the rational design of other heterogeneous catalysts.

关键词： 5-Hydroxymethylfurfural Base-free oxidation Interfacial catalysis Active oxygen species

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Numerical study on the hydrodynamics of agglomerates at intermediate Reynolds numbers

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Chinese Journal of Chemical Engineering 2020年第6期28卷 1533-1541页

作者： Huanpeng Liu Shaowei Wang chenyu yang Taiping Chen School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China

The flow pattern and hydrodynamics of a heterogeneous permeable agglomerate in a uniform upward flow at intermediate Reynolds numbers(1–40)are analyzed from three-dimensional(3 D)computational fluid dynamics *** from the homogeneous or stepwise-varying permeability models used in previous papers,a continuously radially varying permeability model is used in the present *** effects of two dimensionless parameters,the Reynolds number and the permeability ratio,on the flow field and the hydrodynamics were investigated in *** results reveal that unlike the solid sphere,a small recirculating wake initially forms inside the *** critical Reynolds number for the formation of the recirculating wake is lower than that of the solid sphere and it decreases with the increase of permeability ratio.A correlation of drag coefficient as a function of the Reynolds number and permeability ratio is *** of drag coefficients obtained by different permeability models show that at intermediate Reynolds numbers(1–40),the effect of radially varying permeability on the drag coefficient must be considered.

关键词： Agglomerate Permeability Reynolds number Drag coefficient

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