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Conversion of the high-yield salinomycin producer Streptomyces albus BK3-25 into a surrogate host for polyketide production

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Science China(Life Sciences) 2017年第9期60卷 1000-1009页

作者： Xiaojie Zhang chenyang lu Linquan Bai State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology Shanghai Jiao Tong University School of Marine Sciences Ningbo University

An ideal surrogate host for heterologous production of various natural products is expected to have efficient nutrient utilization,fast growth,abundant precursors and energy supply,and a pronounced gene *** albus BK3-25 is a high-yield industrial strain producing type-Ⅰ polyketide sahnomycin,with a unique ability of bean oil *** potential of being a surrogate host for heterologous production of PKS was engineered and evaluated ***,introduction of a three-gene cassette for the biosynthesis of ethylmalonyl-CoA resulted in accumulation of ethylmalonyl-CoA precursor and sahnomycin,and subsequent deletion of the sahnomycin biosynthetic gene cluster resulted in a host with rich supplies of common polyketide precursors,including malonyl-CoA,methylmalonyl-CoA,and ***,the energy and reducing force were measured,and the improved accumulation of ATP and NADPH was observed in the ***,the strength of a series of selected endogenous promoters based on microarray data was assessed at different growth phases,and a strong constitutive promoter was identified,providing a useful tool for further engineered gene ***,the potential of the BK3-25 derived host ZXJ-6 was evaluated with the introduction of the actinorhodin biosynthetic gene cluster from Streptomyces coelicolor,and the heterologous production of actinorhodin was *** work clearly indicated the potential of the high-yield sahnomycin producer as a surrogate host for heterologous production of polyketides,although more genetic manipulation should be conducted to streamline its performance.

关键词： surrogate host heterologous production polyketide salinomycin

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Comparative proteomic analysis reveals the effects of different fatty acid forms on high-fat diet mice

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Food Science and Human Wellness 2022年第2期11卷 316-322页

作者： Jiaping Pan Huan Wang Ye Li chenyang lu School of Marine Science Ningbo UniversityNingbo 315832China Collage of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315832China

Long-chain omega-3 polyunsaturated fatty acids(LC-PUFAs),known for having many health benefits,are usually present in three forms:triglycerides(TG),ethyl esters(EE),and phospholipid(PL).In this study,the effects of these three LC-PUFAs forms(fish oil for TG and EE,krill oil for PL)on the obese mice were compared,and the proteomic changes that focused on lipid metabolism were evaluated via label-free quantitative proteomics *** with the model group,all three of the LC-PUFA form supplementations(labeled as the FO-TG group,FO-EE group and KO-PL groups)could significantly reduce body weight gain(P<0.01).Low-density lipoprotein cholesterol levels were significantly decreased,whereas high-density lipoprotein cholesterol levels were significantly increased in the FO-TG group and FO-EE group(P<0.01),and especially in the PL group(P<0.001).Furthermore,proteomics analysis results suggested that some differentially expressed genes involved in the fatty acid degradation and oxidation pathways had a higher expression fold in the KO-PL group than in the FO-TG or FO-EE *** results showed that dietary LC-PUFAs can reduce fat deposition and inhibit lipogenesis in the liver by upregulating the expression of proteins that are involved in the fatty acid degradation and oxidation ***,KO-PL elicits stronger effects than FO-TG or FO-EE.

关键词： LC-PUFAs Fish oil Krill oil Label-free quantitative proteomics Lipids metabolism

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Chinese Torreya grandis *** seed oil affects obesity through accumulation of sciadonic acid and altering the composition of gut microbiota

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Food Science and Human Wellness 2022年第1期11卷 58-67页

作者： Huan Wang Ye Li Rui Wang Huaifei Ji chenyang lu Xiurong Su State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety ofAgro-products Ningbo UniversityNingbo 315211China Collage of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315800China Collage of Marine Science Ningbo UniversityNingbo 315800China

Torreya grandis ***(Taxace,Torreya)is mainly distributed across the hilly areas of subtropical China and is well known for its nutritional *** this study,the ameliorative effects of *** seed oil on lipid metabolism were investigated,and the underlying mechanism was explored from the perspective of gut *** experiments showed that the rate of body mass gain in the group where the mice were fed a high-fat diet(HFD)and supplemented with 550 mg/(kg·day)*** seed oil(HFD+TO550 group)was 42.27%,while it was 62.25%in the HFD *** with the HFD group,the liver and fat indices,total cholesterol,triglycerides,and low-density lipoprotein cholesterol were reduced in the oil-supplement ***,the oil supplement significantly changed the fatty acid composition and alleviated pathological damage to the liver caused by the high-fat ***,the distinct clustering of bacteria in the composition of gut microbiota was observed in the oil treatment group compared with that in the HFD *** seed oil significantly increased the abundance of the beneficial bacteria and short-chain fatty acid producers,including Lactobacillus,Bifidobacterium,Faecalibaculum and *** results suggest that the supplements of *** seed oil could alleviate hyperlipidemia caused by *** positive effects are considered to be related with sciadonic acid(SCA)and are partially mediated by alterations in gut microbiota composition and functionality.

关键词： Torreya grandis cv.Merrillii seed oil Obesity Lipid metabolism Gut microbiota

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Wireless whispering-gallery-mode sensor for thermal sensing and aerial mapping

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Light(Science & Applications) 2018年第1期7卷 440-445页

作者： Xiangyi Xu Weijian Chen Guangming Zhao Yihang Li chenyang lu Lan Yang Department of Electrical and Systems Engineering Washington UniversitySt.LouisMO 63130USA Department of Computer Science and Engineering Washington UniversitySt.LouisMO 63130USA

The Internet of Things(IoT)1,2 employs a large number of spatially distributed wireless sensors to monitor physical environments,e.g.,temperature,humidity,and air pressure,and has many applications,including environmental monitoring3,health care monitoring4,smart cities5,and precision agriculture.A wireless sensor can collect,analyze,and transmit measurements of its environment1,***,wireless sensors used in the IoT are predominately based on electronic devices that may suffer from electromagnetic interference in many *** immune to the electromagnetic interference,optical sensors provide a significant advantage in harsh ***,by introducing optical resonance to enhance light–matter interactions,optical sensors based on resonators exhibit small footprints,extreme sensitivity,and versatile functionalities7,8,which can significantly enhance the capability and flexibility of wireless *** we provide the first demonstration of a wireless photonic sensor node based on a whisperinggallery-mode(WGM)optical resonator,in which light propagates along the circular rim of such a structure like a sphere,a disk,or a toroid by continuous total internal *** sensor node is controlled via a customized iOS *** performance was studied in two practical scenarios:(1)real-time measurement of the air temperature over 12 h and(2)aerial mapping of the temperature distribution using a sensor node mounted on an unmanned *** work demonstrates the capability of WGM optical sensors in practical applications and may pave the way for the large-scale deployment of WGM sensors in the IoT.

关键词： IoT mode mapping

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Unconventional energetics of small vacancy clusters in BCC high-entropy alloy Nb_(0.75)ZrTiV_(0.5)

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Journal of Materials Science & Technology 2023年第15期146卷 61-71页

作者： Tan Shi Xi Qiu Yundi Zhou Sixin Lyu Jing Li Dan Sun Qing Peng Yong Xin chenyang lu School of Nuclear Science and Technology Xi’an Jiaotong UniversityXi’an 710049China Nuclear Power Institute of China Chengdu 610213China State Key Laboratory of Nonlinear Mechanics Institute of MechanicsChinese Academy of SciencesBeijing 100190China School of Engineering Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong UniversityXi’an 710049China

The stability of small vacancy clusters including divacancy,trivacancy and tetravacancy has been studied in body-centered cubic high-entropy alloy Nb_(0.75)ZrTiV_(0.5) in structures of random solid solution and short-range order by first-principles calculations and molecular dynamics *** from conventional body-centered cubic metals,the tightly bound configurations have a lower structural stability and are not preferred energetically in the studied high-entropy *** of vacancy configurations leads to vacancy-atom exchanges that favor less compact *** formation energy of small vacancy clusters is much smaller than its constituent elements of Nb and V due to the large structural adjustment induced by severe local lattice *** difference in local lattice distortion and elemental arrangement in the vacancy neighborhood leads to significant site-to-site variation in vacancy cluster energy and *** formation energy has a strong correlation with the local energy state of the vacancy configuration and the extent of structural *** to random solid solution,the structure of short-range order has a higher stability for the most compact cluster configurations and tends to have higher vacancy cluster formation *** to classical molecular dynamics simulations of cluster diffusion at high temperature,the studied high-entropy alloy has a higher probability of cluster dissociation compared to Nb and *** unconventional energetics of small vacancy clusters is expected to have a profound impact on their generation,diffusion,dissociation,coalescence,as well as the defect microstructure evolution during irradiation.

关键词： Refractory high-entropy alloys Small vacancy clusters First-principles calculations Defect energetics Radiation defect evolution

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Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites

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Food Science and Human Wellness 2024年第3期13卷 1336-1347页

作者： Ziyan Wang Qiuyue Shi Ying Feng Jiaojiao Han chenyang lu Jun Zhou Zhonghua Wang Xiurong Su State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingbo 315000China School of Marine Science Ningbo UniversityNingbo 315000China Key Laboratory of Aquaculture Biotechnology(Ningbo University) Ministry of EducationNingbo UniversityNingbo 315000China College of Life Sciences Tonghua Normal UniversityTonghua 134000China Shandong Beiyou Biotechnology Co. Ltd.Weifang 261000China

Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and *** recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research *** study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV(Pro-Lys-Lys-Val-Val)of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum ***,the polypeptide composition of *** cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS).Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α(TNF-α)than the original *** animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC;affect serum metabolites through the arachidonic acid,glycerophospholipid and linoleic acid metabolism pathways;and further alleviate UC *** study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.

关键词： Ulcerative colitis Rhopilema esculentum Kishinouye Cnidoblasts Marine bioactive polypeptides Metagenome Serum metabolome

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Prognostic value of lymphocyte count in severe COVID-19 patients with corticosteroid treatment

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Signal Transduction and Targeted Therapy 2021年第4期6卷 1098-1100页

作者： chenyang lu Yi Liu Bo Chen Hang Yang Huifang Hu Yi Liu Yi Zhao Department of Rheumatology and Immunology West China HospitalSichuan UniversityChengduChina

Dear Editor,The outbreak of coronavirus disease 2019(COVID-19)has so far caused over 108.2 million confirmed cases and over 2.3 million deaths all over the world as of February 14,2021.1 Among in-hospital patients with COVID-19,the mortality was approximately 28%,however,the percentage increased to over 60%among critically ill patients,and over 80%among those who require mechanical ventilation.2 Treatment of these severe patients is becoming one of the major challenges.

关键词： patients mortality treatment

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Characteristics and functions of Limosilactobacillus reuteri(Lactobacillus reuteri)from the intestine of mice supplemented with three dietary components based on genome resequencing and metabolite analysis

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Food Bioscience 2023年第3期53卷 1983-1992页

作者： Ziyan Wang Ying Feng Jiaojiao Han chenyang lu Jun Zhou Zhonghua Wang Xiurong Su State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingboChina School of Marine Science Ningbo UniversityNingboChina Key Laboratory of Aquaculture Biotechnology(Ningbo University)Ministry of Education Ningbo UniversityNingboChina College of Life Sciences Tonghua Normal UniversityTonghuaChina Shandong Beiyou Biotechnology Co. LtdWeifangChina

Limosilactobacillus reuteri is a resident probiotic in the intestines of animals with strong environmental *** the current study,to elucidate the adaptation strategies of *** to the intestine of mice supplemented with three dietary components(oil,peptide and cellulose),whole genome resequencing and determination of intracellular metabolites were carried *** resequencing results showed that *** TO60(LR-TO)from the intestines of mice supplemented with high docosahexaenoic acid(DHA)tuna oil,or fermented Benincasa hispida culture(*** FB151,abbreviated as LR-FB)had relatively consistent single nucleotide polymorphisms(SNPs)and insertions and deletions(InDels),and those of *** from the intestines of mice supplemented with Rhopilema esculentum polypeptides(*** RE225,abbreviated as LR-RE)were quite different from *** KEGG functional annotation of resequencing and the intracellular metabolites suggested that in all of the *** adapted to the intestinal environment mainly by regulating carbohydrate metabolism and membrane transport metabolic ***,there were still some differences in their genes and metabolites,which may be related to the changes in the intestinal environment caused by three different types of dietary *** study analyzed the adaptation mechanism of *** to different isolated sources at the genetic level and metabolic level,which is helpful to clarify the adaptation strategies of probiotics to the intestinal environment,and provide supplementary information for the use of dietary nutritional supplements.

关键词： Limosilactobacillus reuteri Whole genome resequencing Intracellular metabolites High DHA tuna Oil Fermented Benincasa hispida culture Rhopilema esculentum polypeptides

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A promising new class of irradiation tolerant materials:Ti_2ZrHfV_(0.5)Mo_(0.2) high-entropy alloy

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Journal of Materials Science & Technology 2019年第3期35卷 369-373页

作者： Yiping lu Hefei Huang Xuzhou Gao Cuilan Ren Jie Gao Huanzhi Zhang Shijian Zheng Qianqian Jin Yonghao Zhao chenyang lu Tongmin Wang Tingju Li Key Laboratory of Solidification Control and Digital Preparation Technology (Liaoning Province) School of Materials Science and Engineering Dalian University of Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences (CAS) School of Materials Science and Engineering Nanjing University of Science and Technology Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences Department of Nuclear Engineering and Radiological Sciences University of Michigan

Recently, high-entropy alloys(HEAs) or multi-principal-element alloys with unprecedented physical,chemical, and mechanical properties, have been considered as candidate materials used in advanced reactors due to their promising irradiation resistant behavior. Here, we report a new single-phase bodycentered cubic(BCC) structured Ti_2 ZrHfV_(0.5)Mo_(0.2) HEA possessing excellent irradiation resistance, i.e.,scarcely irradiation hardening and abnormal lattice constant reduction after helium-ion irradiation,which is completely different from conventional alloys. This is the first time to report the abnormal XRD phenomenon of metallic alloys and almost no hardening after irradiation. These excellent properties make it to be a potential candidate material used as core components in next-generation nuclear reactors. The particular irradiation tolerance derives from high density lattice vacancies/defects.

关键词： High-entropy alloy Irradiation resistance Microstructural characterization Defects evolution

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Food-derived bio-functional peptides for the management of hyperuricemia and associated mechanism

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Food Science and Human Wellness 2024年第4期13卷 1767-1786页

作者： Yanxin Wang Mingxia Ma Yumeng Huang Siqing Fan Jie Peng Shiming Li Xiurong Su Yanbo Wang chenyang lu State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products and School of Marine Science Ningbo UniversityNingbo 315211China School of Food and Health Beijing Technology and Business UniversityBeijing 100048China Department of Food Science Rutgers UniversityNew BrunswickNJ 08901USA College of Life Sciences Huanggang Normal UniversityHuanggang 438000China Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

Hyperuricemia,a metabolic disorder related to uric acid metabolism dysregulation,has become a common metabolic disease worldwide,due to changes in lifestyle and dietary *** recent years,owing to their high activity and few adverse effects,food-derived active peptides used as functional foods against hyperuricemia have attracted increasing *** article aims to focus on the challenge associated with peptide-specific preparation methods development,functional components identification,action mechanism(s)clarification,and bioavailability *** current review proposed recent advances in producing the food-derived peptides with high anti-hyperuricemia activity by protein source screening and matched enzymatic hydrolysis condition adjusting,increased the knowledge about strategies to search antihyperuricemia peptides with definite structure,and emphasized the necessity of combining computer-aided approaches and activity *** addition,novel action mechanism mediated by gut microbiota was discussed,providing different insights from classical ***,considering that little attention was paid previously on the structure-activity relationships of anti-hyperuricemia peptides,we collected the sequences from published studies and make a preliminary summary about the structure-activity relationships,which in turn provided guides for enzymatic hydrolysis optimization and bioavailability ***,this article could promote the development,application and commercialization of food-derived anti-hyperuricemia peptides in the future.

关键词： Hyperuricemia Peptide Enzyme hydrolysis Mechanism Structure-activity relationship Bioavailability

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