检索结果-南通市图书馆

LncRNA-AP006284.1 promotes prostate cancer cell growth and motility by forming RNA-DNA triplexes and recruiting GNL3/SFPQ complex to facilitate RASSF7 transcription

引用

Genes & Diseases 2023年第2期10卷 317-320页

作者： Yali Lu Yan Lin Xiaoyang Zhang Jun Yan Zhe Kong Lu Zhang chenji wang Yan Huang Shimin Zhao Yao Li Obstetrics and Gynecology Hospital State Key Laboratory of Genetic EngineeringMOE Engineering Research Center of Gene TechnologySchool of Life ScienceFudan UniversityShanghai 200433China Shanghai Engineering Research Center of Industrial Microorganisms School of Life ScienceFudan UniversityShanghai 200433China Institute of Metabolism and Integrative Biology (IMIB) Key Laboratory of Reproduction Regulation of NPFPCFudan UniversityShanghai 200433China Institutes of Biomedical Sciences Fudan UniversityShanghai 200433China Department of Laboratory Animal Science Fudan UniversityShanghai 200032China

Long noncoding RNAs(lncRNAs)are known to be involved in transcriptional regulation and their deregulation is associated with the development of human diseases such as cancer.1,2 LncRNA can directly bind to purine-rich double-stranded DNA sequences through Hoogsteen base pairing to form an RNA-DNA triplex motifs and regulate gene expression.3,4,5 However,its regulatory mechanisms and functions in tumors remain ***,we report that the LMNTD2 antisense RNA 1(LMNTD2-AS1,also known as AP006284.1)is highly expressed in prostate cancer(PCa)and positively correlated with the expression of its adjacent coding gene N-terminal Ras-association domain family 7(RASSF7).Mechanistically,AP006284.1 tethers to the RASSF7 promoter via RNA-DNA triplexes in cis-acting manner,which enhances chromatin accessibility and recruits the transcription factor complex GNL3/SFPQ to activate the expression of RASSF7,a repressor of the Hippo signaling ***,overexpression of either AP006284.1 or RASSF7 inactivated the Hippo signaling and malignant proliferation of PCa cells.

关键词： expression LncRNA cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of process parameters on interfacial microstructure and mechanical properties of Al/Cu friction stir lap welding joints

引用

China Welding 2022年第4期31卷 48-58页

作者： wang chenji Liu Song Zhu Hao Cao Zhilong Dong Shaokang School of Materials Science and Engineering Shijiazhuang TIEDAO UniversityShijiazhuang 050043China Hebei Key Laboratory of Advanced Materials for Transportation Engineering and Environment Shijiazhuang 050043China

In this study,friction stir lap welding(FSLW)was performed for the welding test of 6061 aluminium alloy and T2 pure *** effect of process parameters containing rotation rate and travel speed on interfacial microstructure evolution and mechanical properties of Al/Cu dissimilar joints were *** experiments were carried out under the rotation rates of 600,900 and 1200 r/min and with the travel speeds of 30,70 and 100 mm/*** characteristic of interface transition zones(ITZs)and the species of intermetallic compounds(IMCs)were *** Al/Cu interface showed a layered structure composed of Al-Cu IMCs,which will affect the mechanical *** layer consisting of Al2Cu was formed at lower heat input,and as heat input increased the Al4Cu9 phase started to *** heat input will increase the thickness of the interface and raise the brittleness of the *** thickness of the IMCs layers changed from0.89μm to 3.96μm as the heat input *** maximum value of tensile shear loading of 4.65 kN was obtained at the rotation rate of900 r/min and travel speed of 100 mm/min with the interface thickness of 2.89μ*** fracture mode of the joints was a mix of ductile and brittle fracture.

关键词： friction stir lap welding Al/Cu dissimilar welding intermetallic compounds process parameters mechanical properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Strength mismatch mechanism on friction stir welding joint of 7075 aluminum alloy

引用

China Welding 2021年第1期30卷 30-36页

作者： Zhu Hao Dong Shaokang Zhao Yipeng wang chenji wang Jun Zhu Shaojin School of Materials Science and Engineering Shijiazhuang TIEDAO UniversityShijiazhuang 050043China Hebei Key Laboratory of Advanced Materials for Transportation Engineering and Environment Shijiazhuang 050043China School of Materials Science and Engineering Hebei University of Science and TechnologyShijiazhuang 050018China China Petroleum Pipeline Engineering Co.LTD Langfang 065000China

The precipitated phases in the WNZ,TMAZ,HAZ and BM of the friction stir welding(FSW)joint were observed using the transmission electron microscopy(TEM)and the lattice fringe spacing of the precipitated phases was *** with X-ray diffraction(XRD),the types of precipitated phases among the joint were confirmed and then the strength mismatch mechanism was *** results show the precipitated phases of 7075 aluminum alloy FSW joint mainly consist of MgZn_(2),AlCuMg and Al_(2)*** microzone of the joint experienced different thermal cycles,the types and sizes of precipitated phases are different and the strengthening effect is *** strengthening effect of the AlCuMg and Al_(2)CuMg are better than that of MgZn_(2).The precipitated phase in the WNZ mainly includes AlCuMg and Al_(2)CuMg,as well as the grain size is fine,the microhardness in this zone is pretty *** number of precipitated phase AlCuMg and Al_(2)CuMg is smaller in the TMAZ and the MgZn_(2)is relatively more,which lead the microhardness *** number of precipitated phase MgZn_(2)is relative larger in the HAZ,as well as the grain coarsening,the microhardness in this zone is lowest of the *** the same time,there are the precipitate free zones(PFZ)among the 7075 aluminum alloy FSW joint,which decreases the microhardness of the whole joint to some extent.

关键词： 7075 aluminum alloy friction stir welding precipitated phase the precipitate free zones strength mismatch

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

医患纠纷主体之诉讼心理研究

引用

医学与法学 2016年第2期8卷 81-83页

作者：王晨霁安徽医科大学马克思主义学院法学系

医患纠纷产生的原因有很多,解决方式也各异,参与其中的各方主体在纠纷产生、发展、解决的过程中更是各有其特殊性。本文研究医患诉讼中各主体诉讼心理的特殊性、诉讼行为与诉讼心理之间的关系,通过揭示医患纠纷诉讼中各主体的诉讼心理... 详细信息

医患纠纷产生的原因有很多,解决方式也各异,参与其中的各方主体在纠纷产生、发展、解决的过程中更是各有其特殊性。本文研究医患诉讼中各主体诉讼心理的特殊性、诉讼行为与诉讼心理之间的关系,通过揭示医患纠纷诉讼中各主体的诉讼心理和预见各主体的诉讼行为,以寻求控制、矫治不良的诉讼心理与行为之途径与方法,以期于实践中有助于医患关系的和谐发展。

关键词：医患纠纷诉讼心理诉讼行为

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Targeted bisulfite sequencing identified a panel of DNA methylation-based biomarkers for esophageal squamous cell carcinoma(ESCC)

Targeted bisulfite sequencing identified a panel of DNA meth...

引用

2017中国长三角遗传学大会

作者： Weilin Pu chenji wang Sidi Chen Dunmei Zhao Yinghui Zhou Yanyun Ma Ying wang Caihua Li Zebin Huang Li Jin Shicheng Guo Jiucun wang Minghua wang State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentSchool of Life Sciences and Institutes of Biomedical SciencesFudan University Department of Biochemistry and Molecular Biology Medical CollegeSoochow University Ministry of Education Key Laboratory of Contemporary Anthropology School of Life SciencesFudan University

Background:DNA methylation has been implicated as a promising biomarker for precise cancer ***,limited DNA methylation-based biomarkers have been described in esophageal squamous cell carcinoma(ESCC).Method:A high-throughput DNA methylation dataset(100 samples) of ESCC from the cancer genome atlas(TCGA) project was analyzed and validated along with another independent dataset(12 samples) from the gene expression omnibus(GEO) *** methylation status of peripheral blood mononuclear cells and peripheral blood leucocytes from healthy controls was also utilized for biomarker *** candidate CpG sites as well as their adjacent regions were further validated in 94 pairs of ESCC tumor and adjacent normal tissues from the Chinese Han population using the targeted bisulfite sequencing *** regression and several machine learning methods were applied for evaluation of the diagnostic ability of our *** the discovery stage,five CpG sites were selected as candidate biomarkers for further analysis as shown below:cg05249644,P=2.20 × 10;cgl5830431,P = 3.60 × 10;cg20655070,P = 3.60 × 10;cg26671652,P = 5.77 × 10;and cg27062795,P = 3.60 × *** the validation stage,the methylation status of both the five CpG sites and their adjacent genomic regions were *** diagnostic model based on the combination of these five genomic regions yielded a robust performance(Sensitivity = 0.75,Specificity = 0.88,AUC = 0.85).Eight statistical models along with five-fold cross-validation were further applied,in which the SVM model reached the best accuracy in both training and test dataset(Accuracy = 0.82 and 0.80,respectively).In addition,subgroup analyses revealed a significant difference in diagnostic performance between the alcohol use and non-alcohol use ***:Methylation profiles of the five genomic regions covering cg05249644(STK3),cgl5830431,cg20655070,cg26671652(ZNF418) and cg27062795(ZNF542) can be used for effective met

关键词： Esophageal squamous cell carcinoma DNA methylation Biomarker Diagnosis Targeted bisulfite sequencing

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification of hyper-methylated tumor suppressor genes-based diagnostic panel for esophageal squamous cell carcinoma(ESCC) in a Chinese Han population

Identification of hyper-methylated tumor suppressor genes-ba...

引用

2017中国长三角遗传学大会

作者： chenji wang Weilin Pu Dunmei Zhao Sidi Chen Yinghui Zhou Zhenglei He Xulong Feng Ying wang Caihua Li Li Jin Shicheng Guo Jiucun wang Minghua wang Department of Biochemistry and Molecular Biology Medical CollegeSoochow University State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentSchool of Life SciencesFudan University Ministry of Education Key Laboratory of Contemporary Anthropology School of Life SciencesFudan University

Background:DNA methylation-based biomarkers were suggested to be promising for early cancer ***,DNA methylation-based biomarkers for esophageal squamous cell carcinoma(ESCC),especially in Chinese Han populations have not been identified and evaluated ***:Candidate tumor suppressor genes(N=65) were selected through literature searching and four public high-throughput DNA methylation microarray datasets including 136 samples totally were collected for initial *** bisulfite sequencing was applied in an independent cohort of 94 pairs of ESCC and normal tissues from a Chinese Han population for eventual ***:ADHFE1,EOMES,SALL1 and TFPI2 were identified and validated in the ESCC samples from a Chinese Han *** four candidate regions were validated to be significantly hypermethylated in ESCC samples(ADHFE1,p = 1.70×10;EOMES,p = 2.90×10;SALLl,p = 3.90×10;TFPI2,p = 3.40 × 10).Logistic regression based prediction model shown a robust ESCC classification performance(Sensitivity = 66%,Specificity = 87%,AUC = 0.81).Moreover,advanced classification method had better performances(random forest and naive Bayes).Interestingly,the diagnostic performance could be improved in non-alcohol use subgroup(AUC = 0.84).Conclusion:Methylation panels of ADHFEl,EOMES,SALL1 and TFPI2 could be an effective methylation-based assay for ESCC diagnosis.

关键词： Esophageal squamous cell carcinoma DNA methylation Biomarker Diagnosis Targeted bisulfite sequencing

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

1000 MPa级高强钢熔敷金属强韧化机理分析

引用

焊接学报 2023年第7期44卷 116-122,I0009,I0010页

作者：曹志龙朱浩安同邦王晨霁马成勇彭云石家庄铁道大学石家庄050043 钢铁研究总院北京100081

自主设计4种不同镍含量(ω_(Ni))的Ni-Cr-Mo系焊丝,采用TIG焊制备1000 MPa级高强钢熔敷金属.利用光学显微镜、扫描电子显微镜、透射电子显微镜、X射线衍射仪等对不同镍含量的熔敷金属微观组织进行表征,通过拉伸、冲击、硬度试验对熔敷... 详细信息

自主设计4种不同镍含量(ω_(Ni))的Ni-Cr-Mo系焊丝,采用TIG焊制备1000 MPa级高强钢熔敷金属.利用光学显微镜、扫描电子显微镜、透射电子显微镜、X射线衍射仪等对不同镍含量的熔敷金属微观组织进行表征,通过拉伸、冲击、硬度试验对熔敷金属力学性能进行测试,探求镍含量对1000 MPa级高强钢熔敷金属强韧性机理的影响规律.结果表明,不同镍含量熔敷金属组织均由板条马氏体、板条贝氏体、联合贝氏体和残余奥氏体组成;镍含量不同,微观组织不同;随着镍含量增加,柱状晶宽度增大,板条马氏体、联合贝氏体和残余奥氏体增多,板条贝氏体减少,熔敷金属强度提高,塑性降低;当ω_(Ni)为5.44%时,强韧匹配最佳,屈服强度为1005 MPa,−50℃下冲击吸收能量为95 J.创新点:阐明了1000 MPa级海工用钢熔敷金属强韧化机理,揭示镍含量对1000 MPa级熔敷金属强韧化机理的影响.

关键词： Ni元素 1000 MPa级熔敷金属微观组织强韧化机理

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

镍中间层对铝/镁异种金属搅拌摩擦焊接头微观组织的影响

引用

焊接学报 2022年第12期43卷 84-89,I0008页

作者：董少康马宇航朱浩王晨霁曹志龙王军石家庄铁道大学石家庄050043 河北省交通工程与环境协同发展新材料重点实验室石家庄050043 河北科技大学石家庄050018

采用搅拌摩擦焊(friction stir welding,FSW),引入厚度为0.05 mm镍箔作为中间层,在焊接速度不变条件下,采用不同转速对厚度为4 mm的6061铝合金和AZ31镁合金进行平板对接,对接头进行系列微观组织表征及力学性能测试,探讨转速对接头中镍... 详细信息

采用搅拌摩擦焊(friction stir welding,FSW),引入厚度为0.05 mm镍箔作为中间层,在焊接速度不变条件下,采用不同转速对厚度为4 mm的6061铝合金和AZ31镁合金进行平板对接,对接头进行系列微观组织表征及力学性能测试,探讨转速对接头中镍颗粒分布状态,金属间化合物(intermetallic compounds,IMCs)种类与分布及接头强度的影响规律.研究结果表明:与未引入中间层接头相比,引入镍改变了铝/镁异种金属FSW接头焊核区(weld nugget zone,WNZ)中IMCs种类及分布,WNZ存在明显的镁合金与铝合金相间的带状组织,其上分布着絮状Al_(12)Mg_(17)、颗粒状Mg_(2)Ni、层状Al_(3)Mg_(2)及大小不一的镍箔颗粒;随着转速增加,镍箔颗粒分布愈加均匀,Al_(3)Mg_(2)数量相对减少,且脆性Al_(3)Mg_(2)由连续分布逐渐演变为断续分布;当转速为750 r/min时,接头抗拉强度达到最大值,与未引入中间层接头相比,引入镍中间层接头抗拉强度提高了56 MPa,达到镁合金的56.9%.

关键词：铝/镁异种金属搅拌摩擦焊镍箔中间层金属间化合物

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

1400 MPa级超高强钢SH-CCT曲线及其热影响区组织和性能

引用

焊接学报 2023年第8期44卷 109-115,I0009,I0010页

作者：曹志龙朱浩安同邦王晨霁马成勇彭云钢铁研究总院北京100081 石家庄铁道大学石家庄050043

采用Formastor-FⅡ全自动相变仪,结合光学显微镜(OM)和维氏硬度测试,研究不同冷却速率对1400 MPa级超高强钢焊接热影响区(HAZ)粗晶区组织转变和性能的影响规律,绘制焊接连续冷却转变曲线(SH-CCT),结合焊接工艺试验,采用公式法确定最佳... 详细信息

采用Formastor-FⅡ全自动相变仪,结合光学显微镜(OM)和维氏硬度测试,研究不同冷却速率对1400 MPa级超高强钢焊接热影响区(HAZ)粗晶区组织转变和性能的影响规律,绘制焊接连续冷却转变曲线(SH-CCT),结合焊接工艺试验,采用公式法确定最佳焊接工艺参数.结果表明,1400 MPa级超高强钢马氏体临界转变冷却速率约为5℃/s,当冷却速率大于5℃/s时,热影响区粗晶区组织为单一板条马氏体,硬度值为487~509 HV5,冷却速率小于5℃/s时,粗晶区组织中出现中高温相变产物,即板条贝氏体、粒状贝氏体和铁素体等组织,硬度值下降为487~260 HV5,同时组织中还出现M-A组元,其含量随冷却速率降低先增加后减少,形态也由弥散颗粒状变为断续长条状或块状分布;厚度8 mm的1400 MPa级超高强钢焊接时,热输入控制在20 kJ/cm以内,粗晶区组织为板条马氏体,硬度维持在500 HV5左右,粗晶区不发生软化现象,满足工程使用要求.

关键词： 1400 MPa级超高强钢冷却转变曲线焊接热影响区组织 M-A组元

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

“对分课堂”在医学院校思想政治理论课教学改革中的应用

引用

安徽开放大学学报 2021年第4期 52-55页

作者：王晨霁安徽医科大学马克思主义学院合肥230032

为了提高医学院校思想政治理论课教学效果,分析了医学院校思政课教学现状,阐述了将“对分课堂”引入医学院校思政课教学的意义,以思想道德修养与法律基础课程为例探索了“对分课堂”在医学院校思政课教学中的应用,探讨了“教师教什么—... 详细信息

为了提高医学院校思想政治理论课教学效果,分析了医学院校思政课教学现状,阐述了将“对分课堂”引入医学院校思政课教学的意义,以思想道德修养与法律基础课程为例探索了“对分课堂”在医学院校思政课教学中的应用,探讨了“教师教什么——知识讲授、教师怎么教——引导和反馈、学生如何学——内化和讨论”等问题。

关键词：医学院校思想政治理论课 “对分课堂” 教学改革

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：