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Somite-specific expression of a novel fibronectin variant FN3 is negatively regulated by SHH

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Chinese Science Bulletin 2002年第21期47卷 1807-1811,1850页

作者： zhao chengtian ZHANG Yu SU Ying MENG Anming Department of Biological Science and Biotechnology The Protein Science Laboratory of the Ministry of EducationTsinghua UniversityBeijing 100084China

Fibronectins (FNs) are major extracellular proteins in blood plasma and many tissues of vertebrates, and play important roles in adhesion, migration and differentiation of cells. We have identified a novel variant (FN3) of fibronectin in zebrafish. FN3 mRNA is abundant, as detected by whole-mount in situ hybridization, in the presomitic mesoderm and the newly formed somites, but less abundant in mature somites. Ectopic expression of Sonic Hedgehog (SHH) results in a decrease of FN3 expression, whereas the expression level of FN3 increases in the flhi mutants that lack the notochord. Our results suggest that FN3 may be involved in the formation of somites, but during somite differentiation its expression needs to be downregulated by signals derived from the axial tissues.

关键词： zebrafish fibronectin somite somitogenesis extracellu-lar matrix SHH flh.

Zebrafish as a Model for Human Ciliopathies

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Journal of Genetics and Genomics 2016年第3期43卷 107-120页

作者： Zhu Song Xiaoli Zhang Shuo Jia Pamela C.Yelick chengtian zhao Institute of Evolution & Marine Biodiversity College of Marine Life Science Ocean University of China Department of Orthodontics Division of Craniofacial and Molecular Genetics Tufts University Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

Cilia, microtubule-based structures found on the surface of almost all vertebrate cells, play an array of diverse biological functions. Abnormal ciliary axonemal structure and function can result in a class of genetic disorders that are collectively termed ciliopathies. Model organisms, including Chlamydomonas reinhardtii and Caenorhabditis elegans have been widely used to study the complex genetic basis of ciliopathies. Here, we review the advantages of the zebrafish as a vertebrate model for human ciliopathies. We summarize the features of zebrafish cilia, and the major findings and contributions of the zebrafish model in recent studies of human ciliopathies. We also discuss the new genome editing approaches being efficiently used in zebrafish, and the exciting prospects of these approaches in modeling human ciliopathies.

关键词： Cilia Zebrafish Ciliopathy Disease model

Cilia regulate meiotic recombination in zebrafish

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Journal of Molecular Cell Biology 2022年第7期14卷 37-48页

作者： Haibo Xie Xiaosi Wang Minjun Jin Lanqin Li Junwen Zhu Yunsi Kang Zhe Chen Yonghua Sun chengtian zhao Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyInnovation Academy for Seed DesignHubei Hongshan LaboratoryChinese Academy of SciencesWuhan 430072China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266003China Sars-Fang Centre Ministry of Education Key Laboratory of Marine Genetics and BreedingCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China

Meiosis is essential for evolution and genetic diversity in almost all sexual eukaryotic *** mechanisms of meiotic recombination,such as synapsis,have been extensively ***,it is still unclear whether signals from the cytoplasm or even from outside of the cell can regulate the meiosis *** are microtubule-based structures that protrude from the cell surface and function as signaling hubs to sense extracellular ***,we reported an unexpected and critical role of cilia during meiotic *** gametogenesis of zebrafish,cilia were specifically present in the prophase stages of both primary spermatocytes and primary *** developing a germ cell-specific CRISPR/Cas9 system,we demonstrated that germ cell-specific depletion of ciliary genes resulted in compromised double-strand break repair,reduced crossover formation,and increased germ cell *** study reveals a previously undiscovered role for cilia during meiosis and suggests that extracellular signals may regulate meiotic recombination via this particular organelle.

关键词： cilia meiosis homologous recombination kif3a zebrafish

Biodiversity-based development and evolution:the emerging research systems in model and non-model organisms

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Science China(Life Sciences) 2021年第8期64卷 1236-1280页

作者： Long zhao Feng Gao Shan Gao Yujun Liang Hongan Long Zhiyi Lv Ying Su Naihao Ye Liusuo Zhang chengtian zhao Xiaoyu Wang Weibo Song Shicui Zhang Bo Dong Institute of Evolution and Marine Biodiversity(IEMB) Ocean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China College of Fisheries Ocean University of ChinaQingdao 266003China College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China CAS Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Evolutionary developmental biology,or Evo-Devo for short,has become an established field that,broadly speaking,seeks to understand how changes in development drive major transitions and innovation in organismal *** does so via integrating the principles and methods of many subdisciplines of *** we have gained unprecedented knowledge from the studies on model organisms in the past decades,many fundamental and crucially essential processes remain a *** the tremendous biodiversity of our planet,the current model organisms seem insufficient for us to understand the evolutionary and physiological processes of life and its adaptation to exterior *** currently increasing genomic data and the recently available gene-editing tools make it possible to extend our studies to non-model *** this review,we review the recent work on the regulatory signaling of developmental and regeneration processes,environmental adaptation,and evolutionary mechanisms using both the existing model animals such as zebrafish and Drosophila,and the emerging nonstandard model organisms including amphioxus,ascidian,ciliates,single-celled phytoplankton,and marine *** addition,the challenging questions and new directions in these systems are outlined as well.

关键词： Biodiversity evolutionary planet

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海洋动物胚胎冷冻保存的研究进展

中国海洋大学学报（自然科学版） 2024年第8期54卷 1-13页

作者：赵呈天任牧阳苑萌中国海洋大学海洋生命学院方宗熙海洋生物进化与发育研究中心山东青岛266071

冷冻保存是一种在生物及医学领域广泛使用的技术手段。自20世纪90年代以来,有关冷冻保存方法的研究逐年增多,其研究方向不仅局限于物种范围,在细胞类型以及应用领域中也呈现出多样化的趋势。随着海洋动物在胚胎学及遗传育种方面研究的... 详细信息

冷冻保存是一种在生物及医学领域广泛使用的技术手段。自20世纪90年代以来,有关冷冻保存方法的研究逐年增多,其研究方向不仅局限于物种范围,在细胞类型以及应用领域中也呈现出多样化的趋势。随着海洋动物在胚胎学及遗传育种方面研究的不断深入,致力于冷冻保存海洋动物胚胎的研究也在增多。本文聚焦于海洋动物胚胎冷冻保存技术,按海洋棘皮动物、海洋软体动物、海洋虾类及海洋鱼类来对海洋动物胚胎进行分类,概述了近年来对30余种海洋动物胚胎冷冻保存研究的进展,分析了海洋动物冷冻保存所面临的技术挑战以及未来的发展趋势。

关键词：冷冻保存海洋动物冷冻保护剂玻璃化胚胎

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斑马鱼神经底板处神经元的分布及特征

遗传 2022年第6期44卷 510-520页

作者：郑鹏飞谢海波朱盼盼赵呈天中国海洋大学海洋生物多样性与进化研究所青岛266003

神经底板(floor plate,FP)位于神经管腹侧中线区,存在多种神经细胞类型,是调控神经管分化及维持体轴生长的重要信号中心。目前,对神经底板处神经元细胞的类型及分布研究并不深入。本研究以斑马鱼(Danio rerio)为模式动物,结合多种神经... 详细信息

神经底板(floor plate,FP)位于神经管腹侧中线区,存在多种神经细胞类型,是调控神经管分化及维持体轴生长的重要信号中心。目前,对神经底板处神经元细胞的类型及分布研究并不深入。本研究以斑马鱼(Danio rerio)为模式动物,结合多种神经细胞的转基因品系,分析了斑马鱼幼鱼中神经底板细胞群的排列图式。研究发现,foxj1a、sox2、clusterin和gfap等多个基因在内侧中央底板(medial floor plate,MFP)单排细胞中表达。Kolmer-Agduhr神经元KAʼ与KA”又称为脑脊液接触神经元,定位在MFP附近,其中KA”神经元表达foxj1a和pkd2l1基因,与表达Gfap蛋白、Olig2蛋白或Sox2蛋白的阳性细胞间隔性插入MFP细胞腹侧间隙中。KAʼ神经元位于KA”背侧,表达foxj1a、pkd2l1和olig2基因,并与Sox2^(+)或Olig2^(+)阳性细胞间隔分布于MFP细胞胞体的两侧。药物处理实验结果表明:抑制Notch信号影响神经底板的发育,导致神经底板细胞排布异常,并引起幼鱼出现体轴上弯表型。本研究初步阐释了斑马鱼幼鱼早期神经底板处各种细胞类型的分布及定位特征,发现Notch信号对神经底板发育的重要调控作用。

关键词：斑马鱼神经底板脑脊液接触神经元 Notch信号

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斑马鱼癌症模型在肿瘤精准医疗中的研究进展

临床与病理杂志 2021年第9期41卷 2193-2200页

作者：张淑慧赵承湉(综述) 冯喜增(审校) 南开大学药物化学生物学国家重点实验室生物活性材料教育部重点实验室生命科学学院天津300071 华南理工大学生物科学与工程学院广州510006

在肿瘤精准医疗中,主要采用两种方法来预测临床相关的肿瘤行为(如治疗响应和药物耐药性的出现):基于患者样本基因组、转录组、表观基因组和/或蛋白质组学进行分析推断,以及在个体化癌症患者“替身”中进行表型研究。后一种方法依赖于小... 详细信息

在肿瘤精准医疗中,主要采用两种方法来预测临床相关的肿瘤行为(如治疗响应和药物耐药性的出现):基于患者样本基因组、转录组、表观基因组和/或蛋白质组学进行分析推断,以及在个体化癌症患者“替身”中进行表型研究。后一种方法依赖于小鼠体内异种移植模型和体外类器官癌症模型或2D细胞培养。患者源性异种移植免疫受损成年斑马鱼品系的建立,以及基于斑马鱼幼鱼异种移植模型药物反应表型测试的第1项临床试验的开展,使斑马鱼这种小脊椎动物应用于精准医疗领域的前沿。

关键词：肿瘤精准医疗斑马鱼转基因癌症模型癌症异种移植模型

Single-Atom Catalysts for Electrochemical Hydrogen Evolution Reaction: Recent Advances and Future Perspectives

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Nano-Micro Letters 2020年第2期12卷 73-101页

作者： Zonghua Pu Ibrahim Saana Amiinu Ruilin Cheng Pengyan Wang chengtian Zhang Shichun Mu Weiyue zhao Fengmei Su Gaixia Zhang Shijun Liao Shuhui Sun Institut National de la Recherche Scientifique-Énergie Matériaux et Télécommunications VarennesQC J3X 1S2Canada State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070People’s Republic of China The Key Laboratory of Fuel Cell Technology of Guangdong Province The Key Laboratory of New Energy Technology of Guangdong UniversitiesSchool of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou 510641People’s Republic of China Key Laboratory of Materials Processing and Mold Ministry of EducationZhengzhou UniversityZhengzhou 450002People’s Republic of China

Hydrogen,a renewable and outstanding energy carrier with zero carbon dioxide emission,is regarded as the best alternative to fossil *** most preferred route to large-scale production of hydrogen is by water electrolysis from the intermittent sources(e.g.,wind,solar,hydro,and tidal energy).However,the efficiency of water electrolysis is very much dependent on the activity of ***,designing high-effective,stable,and cheap materials for hydrogen evolution reaction(HER)could have a substantial impact on renewable energy ***,single-atom catalysts(SACs)have emerged as a new frontier in catalysis science,because SACs have maximum atom-utilization efficiency and excellent catalytic reaction *** synthesis methods and analytical techniques have been adopted to prepare and characterize these *** this review,we discuss recent progress on SACs synthesis,characterization methods,and their catalytic ***,we highlight their unique electrochemical characteristics toward ***,the current key challenges in SACs for HER are pointed out and some potential directions are proposed as well.

关键词： Single-atom catalysts Nanomaterials Electrocatalyst Hydrogen evolution reaction Electrochemical energy conversion

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基于电容与磁复合的装甲目标近程探测方法

兵工学报 2021年第11期42卷 2310-2320页

作者：宋承天焦永晖刘向赵宏宇北京理工大学机电学院北京100081 战略支援部队航天系统部后勤部北京100089

针对低伸弹道复杂环境下对坦克反应装甲的精准定距问题,提出基于电容与磁复合的坦克目标探测方法,其中,电容探测进行精确定距,磁探测进行坦克铁磁目标识别,复合体制解决低伸弹道复杂背景环境的干扰问题。理论分析电容探测原理,通过COMSO... 详细信息

针对低伸弹道复杂环境下对坦克反应装甲的精准定距问题,提出基于电容与磁复合的坦克目标探测方法,其中,电容探测进行精确定距,磁探测进行坦克铁磁目标识别,复合体制解决低伸弹道复杂背景环境的干扰问题。理论分析电容探测原理,通过COMSOL仿真对双电极电容探测模型的电极分布进行优化,有效提高了作用精度。理论推导坦克磁场计算式,对坦克目标周围磁场强度特性进行仿真,基于隧道磁阻设计磁探测电路并进行了实验验证;设计基于状态机的电容与磁复合目标探测识别方法,利用多特征量提高了对目标的识别准确性。开展样机实验验证,结果表明,该复合探测方法可实现对坦克目标的准确识别,达到了1 m±0.2 m的定距要求。同时针对对抗四代以上反应装甲探测距离不足的问题,设计单电极自电容探测模型及电路,进行仿真分析,结果表明,探测距离可提高至2 m以上。

关键词：反坦克反应装甲复合探测电容探测磁探测