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中国超声医学杂志 2014年第9期30卷 845-848页

作者：李茜邓旦陈重吴晓波陶杰赵恒成都军区总医院超声科

目的探讨猪肢体软组织枪弹伤的超声成像特点及变化规律。方法利用53式滑膛枪建立猪下肢软组织枪击伤模型,伤后在不同时相点应用超声动态观察其枪弹致伤区域,并对比分析相应区域病理检查结果。结果 1.超声显示原发伤道始终清晰,周围软组... 详细信息

目的探讨猪肢体软组织枪弹伤的超声成像特点及变化规律。方法利用53式滑膛枪建立猪下肢软组织枪击伤模型,伤后在不同时相点应用超声动态观察其枪弹致伤区域,并对比分析相应区域病理检查结果。结果 1.超声显示原发伤道始终清晰,周围软组织可明显区分。形态表现:迂曲、内径不一、内部回声随时间延长而发生变化的管道状回声。伤道边缘软组织主要回声改变为纹理紊乱,可见细条状或团状无回声或回声不均区。2.(1)伤道内可见气体回声,同时还可沿伤道周边筋膜游走至周围乃至较远组织;(2)伤道内及远隔肌间隙内的气体强回声随致伤时间延续逐渐消失;(3)向伤道内注入生理盐水后,邻近伤道的筋膜随之被掀开,两者相互连通,伤道周围气体强回声也与伤道相通。结论猪肢体软组织枪弹伤超声成像具有诸多特征性表现,其中超声显示枪弹伤后气体可沿筋膜游走至周围组织的这种特殊表现,为伤道周围组织及筋膜损伤提供了佐证。超声技术在枪弹伤中的应用将为临床处理,特别是清创术的实施和术后通畅引流提供重要的影像学保障及依据,有利于提高肢体枪弹伤情判断和治疗,具有良好的应用前景。

关键词：超声检查模型肢体枪弹伤伤道

结核杆菌的细菌学、分子生物学和免疫学检测方法评价

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四川医学 2011年第5期32卷 756-758页

作者：邹自英朱冰汤雪晴汪璐成都军区总医院检验科四川成都610083

目的比较涂片法、荧光定量PCR方法和结核抗体检测法对结核病的诊断价值。方法选取2006年7月~2010年8月期间临床确诊或怀疑结核的检样326例,进行涂片抗酸染色、荧光定量PCR检测和采集血样进行结核抗体检查。结果 326例检样中,检出TB-DNA... 详细信息

目的比较涂片法、荧光定量PCR方法和结核抗体检测法对结核病的诊断价值。方法选取2006年7月~2010年8月期间临床确诊或怀疑结核的检样326例,进行涂片抗酸染色、荧光定量PCR检测和采集血样进行结核抗体检查。结果 326例检样中,检出TB-DNA阳性107例,阳性率32.82%。其中脓液、支气管灌洗液和痰液最高,分别为48.84%（86例）、31.71%（82例）、30.39%（102例）。抗酸染色标本痰液阳性率为9.80%,脓液25.58%,支气管灌洗液15.85%。结核抗体阳性率52.45%。抗酸染色法与荧光PCR法的符合率81.60%,而结核抗体检测法与抗酸染色法的符合率61.97%,与荧光PCR检测法的符合率为80.37%。结论三种方法中,以结核抗体法阳性率最高,荧光PCR法次之,抗酸染色法阳性率最低。三种方法联合运用,可弥补单独采用1种方法阳性率低的缺点,几种方法之间可相互佐证,从而使准确性得以提高。

关键词：结核杆菌检测方法

胆胰合流部解剖特点与急性胆石性胰腺炎的关系

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中华实验外科杂志 1998年第5期15卷 392-394页

作者：黎冬暄田伏洲李红王庆堂黄大熔胡建中成都军区总医院全军普外科中心 610083 成都军区总医院泌尿外科 610083

目的更深入探讨胆胰返流产生的解剖学原因。方法对23例新鲜成人尸体标本进行实验及解剖学观察。结果与结论 (1)正常人中约87%(20/23)胰管开口处和胆管之间菲薄的壶腹隔膜,有"活瓣"的作用,可能是防止胆胰返流的重要结构,该活瓣构造不全者... 详细信息

目的更深入探讨胆胰返流产生的解剖学原因。方法对23例新鲜成人尸体标本进行实验及解剖学观察。结果与结论 (1)正常人中约87%(20/23)胰管开口处和胆管之间菲薄的壶腹隔膜,有"活瓣"的作用,可能是防止胆胰返流的重要结构,该活瓣构造不全者占8%(2/23)。(2)共同通道越长,则胆、胰管汇合位置越高,角度越大,造成"活瓣"样隔膜构造不全的可能性越大,容易产生胆胰返流。这可能是共同通道过长者易发生胆石性急性胰腺炎的原因。(3)十二指肠乳头有良好的防止肠胰返流的功能,提示临床上肠胰返流的情况较少见。

关键词：胆石性胰腺炎病因学解剖学胆胰返流

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绝经前后女性身体成分与骨密度相关性分析

四川医学 2009年第5期30卷 647-649页

作者：游志清万勇郎红梅付静程莹郝新忠邢军叶兴蓉成都军区总医院内分泌科干部病房四川成都610083

目的探讨绝经前后女性身体成分与骨密度(BMD)的关系。方法采用Luna Prodigy双能X线BMD检测仪对健康体检的绝经前女性22例、绝经后女性62例进行腰椎正位,左髋和全身BMD检测和身体成分检测。结果①绝经前组股骨各部位、腰椎(L1～L4)和全身... 详细信息

目的探讨绝经前后女性身体成分与骨密度(BMD)的关系。方法采用Luna Prodigy双能X线BMD检测仪对健康体检的绝经前女性22例、绝经后女性62例进行腰椎正位,左髋和全身BMD检测和身体成分检测。结果①绝经前组股骨各部位、腰椎(L1～L4)和全身BMD,以及全身和躯干肌肉显著高于绝经后组(P0.05);③绝经后组全身和躯干的脂肪、肌肉分别与L3、L4、股骨大粗隆、股骨干和全身BMD均呈显著正相关(P<0.05,或P<0.01);全身脂肪、肌肉,躯干脂肪分别与L1、L2和股骨颈BMD均呈显著正相关(P<0.05,或P<0.01);躯干肌肉与股骨大粗隆骨密度呈显著正相关(P<0.05);左大腿成份与上述部位骨密度均无显著相关性。结论绝经前女性全身BMD与肌肉成分密切相关。绝经后女性全身和躯干脂肪、肌肉与腰椎、股骨和全身BMD密切相关,但脂肪成分似乎更明显。

关键词：双能X线吸收法骨密度身体成分绝经女性

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B7特异性RNA干扰对皮肤移植的影响

四川医学 2009年第6期30卷 807-810页

作者：袁成良邹自英魏世刚李永忠刘定海刘利洪德阳市人民医院检验科烧伤科四川德阳618000 成都军区总医院中心实验室四川成都610083

目的探讨RNA干扰技术阻断B7/CD28共刺激信号对小鼠异体皮肤移植排斥反应的影响。方法设计并合成B7特异性siRNA,在脂质体的介导下转染树突状细胞,转染后72h收集细胞,用蛋白印迹法检测B7-1、B7-2蛋白的表达。在小鼠异体皮肤移植前7d,经静... 详细信息

目的探讨RNA干扰技术阻断B7/CD28共刺激信号对小鼠异体皮肤移植排斥反应的影响。方法设计并合成B7特异性siRNA,在脂质体的介导下转染树突状细胞,转染后72h收集细胞,用蛋白印迹法检测B7-1、B7-2蛋白的表达。在小鼠异体皮肤移植前7d,经静脉将转染B7特异性siRNA的DC输入小鼠体内(DC转染组),同时设立同种异体移植对照组、同系移植对照组、环孢素A(CsA)治疗组(术后每日皮下注射CsA5 mg/kg)和未转染DC组(移植前输注未转染DC),观察各组移植皮瓣的存活时间和排斥反应情况,并用MTT法检测混合淋巴细胞增殖反应,术后1个月观察迟发性超敏反应情况。结果Western Blot结果显示转染72h后,siRNA1对B7-1蛋白表达的抑制率为(77.2±6.26)%;siRNA2对B7-2蛋白表达的抑制率为(73.3±5.42)%。与同种异体移植对照组和未转染DC组比较,DC转染组移植皮瓣成活时间明显延长(P<0.01),组织排斥反应程度较轻,混合淋巴细胞反应和DTH反应显著降低(P<0.01)。结论B7特异性siRNA可明显抑制树突细胞B7基因的表达,以阻断B7/CD28共刺激通路,抑制小鼠皮肤移植排斥反应,为进一步研究siRNA诱导免疫耐受提供了新方法。

关键词： RNA干扰树突状细胞 B7 皮肤移植免疫抑制

Perioperative nutrition support in hepatobiliary and pancreatic surgery

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Hepatobiliary & Pancreatic Diseases International 2009年第1期8卷 9-10页

作者： Ning Li Research Institute of general Surgery,Nanjing general hospital of Nanjing military command,PLA,Nanjing 210002, China Research Institute of general Surgery，Nanjing general hospital of Nanjing military command，PLA，Nanjing 210002，China Li N

Early in 1936,Stduley found that there was a E close relationship between nutritional status and postoperative outcome in surgical *** modern surgery combined with use of prophylactic antibiotics,better anesthesia,improved suture materials and optimal physiotherapy,the rate of postoperative complications in malnourished patients has significantly *** recent studies [1,2]

关键词： Perioperative nutrition support in hepatobiliary and pancreatic surgery body

Immunological tolerance of human hepatocyte xenograft induced by adenovirus vector-mediated CTLA4Ig gene transfer

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Hepatobiliary & Pancreatic Diseases International 2012年第2期11卷 148-153页

作者： Yao-Kai Chen,Xiao-Cong Liu,Jun-Gang Li,Guo-Dong Liu,Yan Guo,Ling Cheng and Yu-Ming Wang Institute of Infectious Diseases, Southwest hospital, Third military Medical University, Chongqing 400038, China and Department of Digestive Diseases, general hospital of chengdu military command, chengdu 610083, China [a] Institute of Infectious Diseases Southwest Hospital Third Military Medical University Chongqing 400038 China [b] Department of Digestive Diseases General Hospital of Chengdu Military Command Chengdu 610083 China

BACKGROUND:Systemic administration of CTLA4Ig has been applied in inducing immunological tolerance of hepatocyte implants,but has potential for systemic immune *** study was designed to induce hepatocyte immunological tolerance by locally expressing CTLA4Ig in an attempt to improve the effectiveness of cell ***:A normal human liver cell line(L02) was transfected with adenovirus vector containing the CTLA4Ig gene(Ad-CTLA4Ig-EGFP) in vitro,and the expression of CTLA4Ig by transfected cells was assessed by fluorescent imaging and immunocytochemical *** cells then were injected into the spleen of Sprague-Dawley rats,the survival of cells was determined by immunohistochemistry,and the immune status was examined through CD4 + and CD69 + T cellcounts and ELISA detection of IL-2 in peripheral ***:L02 cells expressed CTLA4Ig in the cytoplasm for >4 *** L02 cells were observed in the experimental group at 3 and 4 weeks post-transplantation,while none was detected in the control ***,the percentages of CD4 + and CD4 + CD69 + T cells in the CTLA4-transfected group were 24.5% and 45.1%,markedly lower than those in the control group at 4 weeks post-transplantation(P<0.01).Furthermore,the IL-2 level was also lower in the CTLA4transfected group than in the control ***:Adenovirus-mediated CTLA4Ig gene transfer into human hepatocytes has the potential to become an effective method of inducing immunological tolerance in hepatocyte transplantation.

关键词： CTLA4Ig adenovirus vectors hepatocyte transplantation immune tolerance graft rejection

Silencing phospholipid scramblase 1 expression by RNA interference in colorectal cancer and metastatic liver cancer

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Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 393-400页

作者： Wei Cui,Shi-Yong Li,Jun-Feng Du,Zi-Man Zhu and Ping An Department of general Surgery,general Surgery Center of the PLA,general hospital of Beijing military command,Beijing 100700,China Department of Hepatobiliary Surgery,First Affiliated hospital,general hospital of the PLA,Beijing 100037,China [a] Department of general Surgery General Surgery Center of the PLA General Hospital of Beijing Military Command Beijing 100700 China [b] Department of Hepatobiliary Surgery First Affiliated Hospital General Hospital of the PLA Beijing 100037 China

BACKGROUND:Phospholipid scramblase 1(PLSCR1) not only participates in the transbilayer movement of phospholipids,but also plays a role in the pathogenesis and progression of *** present study aimed to evaluate the effect of silencing PLSCR1 expression by RNA interference in colorectal cancer(CRC) and metastatic liver ***:The expression of PLSCR1 in CRC and metastatic liver cancer samples was assessed by *** cultured cells with the highest expression were selected for subsequent *** designed three siRNA oligonucleotide segments targeted at *** transfection was *** biological behavior of the cells in proliferation,adhesion,migration and invasion was ***:PLSCR1 protein expression increased significantly in the majority of CRC and metastatic liver cancer samples compared with normal *** cells had the highest expression of *** siRNA-390 oligonucleotide segment had the best silencing *** transfection,Lovo cell proliferation was significantly inhibited compared with the controls in the MTT *** and fibronectin adhesion assays showed Lovo cell adhesion was also significantly *** the migration assay,the number of migrating cells in the PLSCR1 siRNA-390 group was 50±12,significantly lower than the number in the siRNA-N group(115±28) and in the control group(118±31).In an invasion test,the number of invading cells in the PLSCR1 siRNA-390 group was 60±18,significantly lower than that in the siRNA-N group(97±26) and the control group(103±24).CONCLUSIONS:PLSCR1 is overexpressed in CRC and metastatic liver *** of PLSCR1 by siRNA inhibits the proliferation,adhesion,migration and invasion of Lovo cells,which suggests that PLSCR1 contributes to the tumorigenesis and tumor progression of ***1 may be a potential gene therapy target for CRC and associated metastatic liver cancer.

关键词： phospholipid scramblase 1 colorectal cancer metastatic liver cancer RNA interference

Middle and long-term clinical outcomes of patients with regional hepatolithiasis after subcutaneous tunnel and hepatocholangioplasty with utilization of the gallbladder

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Hepatobiliary & Pancreatic Diseases International 2005年第4期4卷 597-599页

作者： Xu Li, Li Shi, Yu Wang and Fu-Zhou Tian Department of general Surgery, chengdu military general hospital, chengdu 610083, China Department of general Surgery Chengdu Military General Hospital Chengdu 610083 China.

BACKGROUND: Roux-en-Y choledochojejunostomy is routinely performed in patients with regional hepatolithia-sis. However, some of these patients, who have a normal gallbladder and normal Oddi 's sphincter, are unnecessarily undergoing bilio-intestinal drainage. Alternatively, reconstruction can be achieved by subcutaneous tunnel and hepa-tocholangioplasty with the utilization of the gallbladder (STHG). This method is effective to potential endoscopic tunnel and intervention during follow-up, and prevention of reflux cholangitis as well as the disorders of the GI tract. METHODS: The middle and long-term complications of 46 patients who underwent STHG were analyzed. With B-ul-trasonography and biochemical assay, the contraction and concentration function of the gallbladder were also studied. RESULTS: Follow-up showed that all patients survived with a relatively normal life. One patient experienced right epigastric pain, chills and fever because of a stone which impacted in the left hepatic bile duct. Another patient had cholangitis because of biliary ascariasis. The two patients were treated by endoscopic therapy within the subcutaneous gallbladder under local anesthesia. CONCLUSIONS: This operation not only keeps the normal physical functional of the gallbladder, Oddi s sphincter and gastrointestinal tract, but also prevents reflux cholangitis and the disorder of the digestive tract. Hence STHG is a novel operation dealing with regional hepatolithiasis.

关键词： gallbladder biliary tract surgery hepatolithiasis