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OcBSA:An NGS-based bulk segregant analysis tool for outcross populations

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Molecular Plant 2024年第4期17卷 648-657页

作者： Lingkui Zhang Yanfeng Duan Zewei Zhang Lei Zhang Shumin Chen chengcheng cai Shaoguang Duan Kang Zhang Guangcun Li Feng Cheng State Key Laboratory of Vegetable Biobreeding Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural AffairsKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop of Ministry of Agriculture and Rural AffairsSino-Dutch Joint Laboratory of Horticultural GenomicsInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China

Constructing inbred lines for self-incompatible species and species with long generation times is challenging,making the use of F1 outcross/segregating populations the main strategy for genetic studies of such ***,there is a lack of dedicated algorithms/tools for rapid quantitative trait locus(QTL)mapping using the F1 *** this end,we have designed and developed an algorithm/tool called OcBSA specifically for QTL mapping of F1 *** transforms the four-haplotype inheritance problem from the two heterozygous diploid parents of the F1 population into the two-haplotype inheritance problem common in current genetic studies by removing the two haplotypes from the heterozygous parent that do not contribute to phenotype segregation in the F1 *** of OcBSA on 1800 simulated F1 populations demonstrated its advantages over other currently available tools in terms of sensitivity and *** addition,the broad applicability of OcBSA was validated by QTL mapping using seven reported F1 populations of apple,pear,peach,citrus,grape,tea,and *** also used OcBSA to map the QTL for flower color in a newly constructed F1 population of potato generated in this *** OcBSA mapping result was verified by the insertion or deletion markers to be consistent with a previously reported locus harboring the ANTHOCYANIN 2 gene,which regulates potato flower *** together,these results highlight the power and broad utility of OcBSA for QTL mapping using F1 populations and thus a great potential for functional gene mining in outcrossing *** ease of use,we have developed both Windows and Linux versions of OcBSA,which are freely available at:https://***/Bioinformaticslab/OcBSA.

关键词： outcross BSA self-incompatible F1 population QTL mapping potato

中央企业领导人员全方位管理和经常性监督的探索与实践

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当代石油石化 2024年第5期32卷 56-58页

作者：蔡程程中国石油化工集团有限公司党组组织部北京100728

新时代、新征程上,做好央企领导人员全方位管理和经常性监督是护航央企高质量发展的关键所在。通过梳理领导人员全方位管理和经常性监督的内涵要义、经验做法以及存在的问题,尝试提出新形势下加强央企领导人员全方位管理和经常性监督的... 详细信息

新时代、新征程上,做好央企领导人员全方位管理和经常性监督是护航央企高质量发展的关键所在。通过梳理领导人员全方位管理和经常性监督的内涵要义、经验做法以及存在的问题,尝试提出新形势下加强央企领导人员全方位管理和经常性监督的对策建议,探索有效路径,推进全面从严治党向纵深发展。

关键词：干部管理和监督领导人员中央企业全面从严治党

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基于头罩的地铁车辆司机室碰撞生存空间分析

技术与市场 2024年第6期31卷 75-80页

作者：林建鹯陈群蔡程程中车株洲电力机车有限公司湖南株洲412001

基于EN 15227:2020《铁路应用——铁路车辆的耐撞性要求》,以某A型地铁项目为例,不同于以往地铁车辆碰撞安全分析方法,增加了对司机室头罩、前窗玻璃、紧急、疏散门等部件有限元建模,模拟仿真上述部件在列车碰撞时的破坏变形情况,分析... 详细信息

基于EN 15227:2020《铁路应用——铁路车辆的耐撞性要求》,以某A型地铁项目为例,不同于以往地铁车辆碰撞安全分析方法,增加了对司机室头罩、前窗玻璃、紧急、疏散门等部件有限元建模,模拟仿真上述部件在列车碰撞时的破坏变形情况,分析得出司机室头罩等变形未侵入司机室生存空间的研究结论,符合标准要求。

关键词：头罩司机室碰撞生存空间

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马铃薯StSN2信号肽缺失对其定位及蛋白互作的影响

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基因组学与应用生物学 2024年第5期43卷 755-766页

作者：温和蔡诚诚李罗品刘石锋梁晓王强王西瑶四川农业大学西南作物基因资源发掘与利用国家重点实验室成都611130 四川农业大学农学院马铃薯研究与开发中心成都611130 四川省农业科学院作物研究所成都611130

StSN2属于马铃薯(Solanum tuberosum L.)Snakin/GASA(gibberellic acid-stimulated arabidopsis)蛋白家族,其N端具有信号肽,C端具有12个保守的半胱氨酸残基,主要在植物生长发育和逆境胁迫响应方面发挥重要调控作用。为了明确StSN2蛋白N端信号肽区域对其蛋白亚细胞定位及蛋白互作的影响,本研究首先通过信号肽预测及PCR扩增,获得含信号肽的StSN2基因片段和N端信号肽缺失的StSN2△SP片段,长度分别为315 bp和243 bp。其次,通过酶切连接方法将StSN2△SP基因片段连接在亚细胞定位载体上,获得融合蛋白YFP-StSN2△SP。瞬时表达实验结果发现,N端信号肽缺失后的StSN2△SP蛋白的亚细胞定位发生了变化,只定位在细胞核。最后通过同源重组方式将StSN2以及StSN2△SP分别构建到酵母诱饵载体上,并与pGADT7-SnRK1α进行酵母双杂交实验。结果显示,StSnRK1α与StSN2共转化酵母细胞后,其细胞不能在四缺培养基中生长,而StSnRK1α与N端信号肽缺失的StSN2△SP共转化酵母细胞后,其细胞能在四缺培养基中生长并显色,说明StSN2信号肽缺失后对其亚细胞定位及蛋白互作均有影响。本研究为StSN2蛋白的生物学功能研究提供了理论依据,为进一步完善该基因在马铃薯块茎休眠的功能研究奠定了实验基础。

关键词：马铃薯 StSN2 信号肽亚细胞定位酵母双杂交

Brassica rapa Genome 2.0： A Reference Upgrade through Sequence Re-assembly and Gene Re-annotation

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Molecular Plant 2017年第4期10卷 649-651页

作者： chengcheng cai Xiaobo Wang Bo Liu Jian Wu Jianli Liang Yinan Cui Feng Cheng Xiaowu Wang Institute of Vegetables and Flowers Chinese Academy of AgriculturalSciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural Genomics Beijing 100081 P.R. China

Brassica rapa includes many important crops that are cultivated as vegetables, condiments, and oilseeds. Recently, the Brassica genomes have been sequenced extensively： a B. rapa draft reference genome in 2011 （Wang et al., 2011）, a Brassica oleracea in 2014 （Liu et al., 2014）, a Brassica napus in 2014 （Chalhoub et al., 2014）, and Brassica nigra and Brassica juncea in 2016 （Yana et al.. 2016）. The first released B.

关键词： Brassica rapa includes important crops vegetables

On the topological surface states of the intrinsic magnetic topological insulator Mn-Bi-Te family

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National Science Review 2024年第2期11卷 50-64页

作者： Yuan Wang Xiao-Ming Ma Zhanyang Hao Yongqing cai Hongtao Rong Fayuan Zhang Weizhao Chen chengcheng Zhang Junhao Lin Yue Zhao Chang Liu Qihang Liu Chaoyu Chen Shenzhen Institute for Quantum Science and Engineering(SIQSE)and Department of Physics Southern University of Science and Technology(SUSTech)

We review recent progress in the electronic structure study of intrinsic magnetic topological insulators（MnBi2Te4）·（Bi2Te3）n（n = 0, 1, 2, 3） family. Specifically, we focus on the ubiquitously（nearly）gapless behavior of the topological Dirac surface state observed by photoemission spectroscopy, even though a large Dirac gap is expected because of surface ferromagnetic order. The dichotomy between experiment and theory concerning this gap behavior is perhaps the most critical and puzzling question in this frontier. We discuss various proposals accounting for the lack of magnetic effect on the topological Dirac surface state, which are mainly categorized into two pictures, magnetic reconfiguration and topological surface state redistribution. Band engineering towards opening a magnetic gap of topological surface states provides great opportunities to realize quantized topological transport and axion electrodynamics at higher temperatures.

关键词： intrinsic magnetic topological insulator topological surface states magnetic gap magnetic reconfiguration topological surface state redistribution van der Waals spacing expansion

StSN2 interacts with the brassinosteroid signaling suppressor StBIN2 to maintain tuber dormancy

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Horticulture Research 2023年第12期10卷 264-275页

作者： Shifeng Liu chengcheng cai Luopin Li He Wen Jie Liu Liqin Li Qiang Wang Xiyao Wang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130China

After harvest,potato tubers undergo an important period of dormancy,which significantly impacts potato quality and seed ***2 has been reported as a key gene for maintaining tuber dormancy;in this study,we explored the molecular mechanism by which StSN2 maintains ***2 was first identified as a candidate protein that interacts with StSN2 by co-immunoprecipitation/mass spectrometry,and both qPCR and enzyme activity experiments showed that StSN2 can promote the StBIN2 expression and *** addition,the interaction between StSN2 and StBIN2 was verified by yeast two-hybrid,luciferase complementation experiments and *** analysis and site-directed mutagenesis confirmed the critical role of cysteine residues of StBIN2 in its binding to *** to that of StSN2,overexpression of StBIN2 extended the dormancy of potato *** between StSN2 and StBIN2 increased the activity of the StBIN2 enzyme,inhibited the expression of StBZR1,and suppressed BR *** the contrary,this interaction promoted the expression of StSnRK2.2/2.3/2.4/2.6 and StABI5,key genes of ABA signaling,and the phosphorylation of StSnRK2.3,thereby promoting ABA ***,our results indicate that StSN2 interacts with StBIN2 through key cysteine residues and StBIN2 maintains tuber dormancy by affecting ABA and BR *** of this research offer new insights into the molecular mechanism by which StSN2 maintains potato tuber dormancy through interaction with StSIN2 and provide guidance for potato improvement.

关键词： dormancy maintain fir

Genome-wide transcriptome analysis reveals molecular pathways involved in leafy head formation of Chinese cabbage(Brassica rapa)

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Horticulture Research 2019年第1期6卷 95-107页

作者： XiaoXue Sun Ram Kumar Basnet Zhichun Yan Johan Bucher chengcheng cai Jianjun Zhao Guusje Bonnema Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei Collaborative Innovation Center of Vegetable Industry in HebeiDepartment of HorticultureHebei Agricultural UniversityBaoding 071001China Plant Breeding Wageningen University and ResearchWageningen 6708PBThe Netherlands Quantitative genetics department Rijk Zwaan Breeding B.V.Eerste Kruisweg 9Fijnaart 4793 RSThe Netherlands

Chinese cabbage plants go through seedling and rosette stages before forming their leafy *** cabbage plants resemble pak-choi plants at their seedling stage,but in their rosette stage the leaves of Chinese cabbage differentiate,as they increase in size with shorter *** order to understand the molecular pathways that play a role in leafy head formation,transcript abundance of young emerging leaves was profiled during development of two Chinese cabbage genotypes and a single pak-choi *** two Chinese cabbages differed in many aspects,among others earliness,leaf size and shape,leaf numbers,and leafy head ***-wide transcriptome analysis clearly separated the seedling stages of all three genotypes together with the later stages from pak-choi,from the later developmental stages of both Chinese cabbages(rosette,folding,and heading).Weighted correlation network analysis and hierarchical clustering using Euclidean distances resulted in gene clusters with transcript abundance patterns distinguishing the two Chinese cabbages from *** clusters included genes with transcript abundance affected by both genotype and developmental stage,whereas two clusters showed only genotype *** included a genotype by developmental stage cluster highly enriched with the MapMan category photosynthesis,with high expression during rosette and folding in Chinese cabbages and low expression in the heading inner leaves that are not exposed to *** other clusters contained many genes in the MapMan categories Cell,showing again differences between pak-choi and both Chinese *** discuss how this relates to the differences in leaf blade growth between Chinese cabbage and pak-choi,especially at the rosette ***,comparison of the transcriptome between leaves of two very different Chinese cabbages with pak-choi during plant development allowed the identification of specific gene categories associated with leafy head formation.

关键词： seedling heading analysis

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反激变换器缓冲回路的设计

电子器件 2013年第4期36卷 469-472页

作者：蔡程程秦会斌杭州电子科技大学CAE研究所杭州310018

变压器是反激式开关电源的核心,但在开关管关断过程中,变压器的漏感较大,能够引起较高的输出电压尖峰。通过分析反激变换器,旨在减少尖峰电压对电路的影响。文章比较RCD缓冲回路与LCD吸收回路:RCD缓冲电路的箝位电压随电阻减小而减小,... 详细信息

变压器是反激式开关电源的核心,但在开关管关断过程中,变压器的漏感较大,能够引起较高的输出电压尖峰。通过分析反激变换器,旨在减少尖峰电压对电路的影响。文章比较RCD缓冲回路与LCD吸收回路:RCD缓冲电路的箝位电压随电阻减小而减小,但损耗增大;LCD缓冲电路的LC谐振频率要求小于开关频率;能量回馈缓冲电路的箝位电压较低,不需要额外的电感。最终给出了LCD吸收回路的设计方法,以达到较好的效果来减小尖峰电压对电路性能的影响。

关键词：电路性能变压器缓冲回路 RCD LCD