检索结果-南通市图书馆

Early expression of surfactant proteins D in Fusarium solani infected rat cornea

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2012年第3期5卷 297-300页

作者： cheng-Ye che, Gui-Qiu Zhao Department of Ophthalmology the Affiliated Hospital of Qingdao University Qingdao 266003 Shandong Province China Binzhou Hubin Eye Hospital Binzhou 256600 Shandong Province China

AIM: To investigate the early expression of surfactant proteins D(SP-D) in Fusarium solani infected rat cornea. METHODS: Wistar rats were divided into group A, B and C randomly. The right eyes were chosen as the experiment one. Group A was control group. Group B was not inoculated with Fusarium solani. Group C was taken as fusarium solani keratitis model. Five rats in group B and C were executed randomly at 6, 12, 24, 48 and 96 hours respectively after the experimental model being established. The expression of SP-D was assessed through immunohistochemistry and reverse transcription polyrnerase chain reaction(RT-PCR). RESULTS: RT-PCR detected that the SP-D mRNA expression was low in the corneal of normal rats and group B. The expression of fungal infected cornea increased gradually and reached the peak at 24 hours in group C. The synchronous expression of group B and C were in significant difference (Pchemisty discovered the protein of SP-D expression was increased gradually from 12 hours and reached the peak at 48 hours in group C. The synchronous expression of group B and C were also in significant difference (P<0.01). CONCLUSION: There exists SP-D in rat corneal tissue and the expression is significantly increased at the early period of fusarium solani infected cornea. SP-D may play a role in the early innate immunity response of the corneal resistance to Fusarium solani infection.

关键词： keratitis Fusarium solani surfactant protein D innate immune

Spin-modulated near-infrared-Ⅰ emission in a xanthene-decorated Fe(Ⅱ) spin-crossover complex

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China chemistry 2025年

作者： cheng Yi Duyong chen Shihui Zhang Rui Cai Chunying Duan Yinshan Meng Tao Liu State Key Laboratory of Fine chemicals Frontier Science Center for Smart Materials and Chemical Engineering School of Chemical Engineering Dalian University of Technology Instrumental Analysis Center Dalian University of Technology School of chemistry Dalian University of Technology Liaoning Binhai Laboratory

Molecular materials showing synergetic coupling of near-infrared （NIR） fluorescence and spin-crossover （SCO） are promisingly applicable in in-vivo bioimaging,temperature sensing,and spintronic *** the related study faces formidable *** this work we graft the NIR fluorophore xanthene onto the dipyridyl substituted triazole ligand and construct a new Febased SCO material,{FeⅡ（ddxc-abpt）2[N（CN）2]2}(1,ddxc-abpt:（E）-4-(((3,5-di（pyridin-2-yl）-4H-1,2,4-triazol-4-yl)imino)methyl)-N,N-diethyl-2,3-dihydro-1H-xanthen-6-amine).Magnetic studies indicate that compound 1 exhibits thermally induced SCO property in both the solid and solution ***-temperature fluorescence emission spectra reveal a remarkable 7-fold increase in photoluminescence intensity upon the transition from the low-spin state at 110 K to the high-spin state at 280 K,which is the record of all reported coupled fluorescence-SCO ***-temperature ultraviolet-visible （UV-Vis）absorption spectra show a significant change in absorption intensity within the fluorescence emission window,consistent with the F?rster resonance energy transfer （FRET） ***-dependent density functional theory （TD-DFT） calculations demonstrate that the strong coupling is attributed to disrupted energy transfer between the xanthene fluorophore and high-spin *** work provides a feasible approach for expanding the synergetic SCO-fluorescence materials to the NIR-Iwavelength region,and hopefully contributes to the applications of tunable NIR-SCO molecule-based sensors and devices.

关键词：

Transient chemical-mediated epigenetic modulation confers unrestricted lineage potential on human primed pluripotent stem cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年

作者： Shi chen Yuanyuan He Lejun Lv Bei Liu cheng Li Hongkui Deng Jun Xu Department of Cell Biology School of Basic Medical Sciences Peking University Stem Cell Research Center Peking University Health Science Center Peking University Academy of Advanced Interdisciplinary Studies Peking University BeiCell Therapeutics School of Life Sciences Center for Bioinformatics Center for Statistical Science Peking University MOE Engineering Research Center of Regenerative Medicine School of Basic Medical Sciences State Key Laboratory of Natural and Biomimetic Drugs Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation College of Life Sciences Peking-Tsinghua Center for Life Sciences Peking University

Human primed pluripotent stem cells are capable of generating all the embryonic ***,their extraembryonic trophectoderm potentials are *** remains unclear how to expand their developmental potential to trophectoderm *** we show that transient treatment with a cocktail of small molecule epigenetic modulators imparts trophectoderm lineage potentials to human primed pluripotent stem cells while preserving their embryonic *** chemically treated cells can generate trophectoderm-like cells and downstream trophoblast stem cells,diverging into syncytiotrophoblast and extravillous trophoblast *** and CUT&Tag analyses reveal that these induced cells share transcriptional profiles with in vivo trophectoderm and cytotrophoblast,and exhibit reduced H3K27me3 modification at gene loci specific to trophoblast lineages compared with primed pluripotent *** exploration highlighted the critical roles of epigenetic modulators HDAC2,EZH1/2,and KDM5s in the activation of trophoblast lineage *** findings demonstrate that transient epigenetic resetting activates unrestricted lineage potential in human primed pluripotent stem cells,and offer new mechanistic insights into human trophoblast lineage specification and in vitro models for studying placental development and related disorders.

关键词：

The Effect of Glycation of CD59 on Complement-Mediated Cytolysis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2005年第4期2卷 313-317页

作者： ying cheng Meihua Gao Department of Immunology Medical College of Qingdao University Qingdao Shandong China

Vascular proliferation is one of the major causes for morbidity and mortality in diabetes. However, the cellular and molecular mechanisms that link hyperglycemia to this complication remain unclear. In present study, we demonstrated by site-directed mutagenesis that mutated CD59 was more susceptible to glycation-inactivation for hyperglycemia. Mutated and wild-type CD59s were stably expressed in Chinese hamster ovary cells using the pALTER-MAX mammalian expression vector. Western blot, FACS and immunological fluorescence were conducted to confirm that CD59s were tethered to the plasma membrane. Compared to wild-type CD59, human CD59 mutants led to a significant increase in dye release assay. These results indicate that there may be some mutations of CD59 in diabetes population and the mutated CD59, which is more likely to be of glycation than the wild-type, may help to explain the distinct propensity of diabetes subjects to develop vascular proliferation complications.

关键词： CD59 site-directed mutagenesis eukaryotic expression vector

Measuring the tilt of primordial gravitational-wave power spectrum from observations

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2019年第11期62卷 135-137页

作者： Jun Li Zu-cheng che Qing-Guo Huang CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Synergetic Innovation Center for Quantum Effects and Applications Hunan Normal UniversityChangsha 410081China

Primordial gravitational waves (GWs) encode the information about inflation [1], and the power spectrum of primordial GWs have attracted a lot of attentions. There are in general two crucial properties for the primord... 详细信息

关键词： PTA BK Measuring the tilt of primordial gravitational-wave power spectrum from observations

Feasibility Study on Fabrication of Geopolymer Bricks by Wasted Grinding Wheel at Room Temperature

在线全文

学校读者我要写书评

暂无评论

Journal of Renewable Materials 2020年第12期8卷 1657-1670页

作者： Yi-che Hsieh Ta-Wui cheng Chia-Ho Wu Department of Materials and Mineral Resources Engineering National Taipei University of TechnologyTaipei10608Taiwan Institute of Mineral Resources Engineering National Taipei University of TechnologyTaipei10608Taiwan

In this study,the feasibility of producing eco-friendly bricks by using geopolymer technology and a waste grinding wheel(WGW)from the grinding wheel industries was ***,in order to meet industrial needs,for instance,in Taiwan,approximately 500,000 grinding wheels are used *** is,a large number of“waste”grinding wheels are ***,few studies have been conducted on the use of WGWs as raw materials in geopolymer *** use of geopolymer technology to form bricks can avoid the utilization of clay and cement and even prevent the use of a high-temperature process in ***,it can decrease CO_(2) emission and energy consumption and thus,protect the *** this study,the following three major factors were considered:press-forming pressure(70 and 100 kgf/cm2),NaOH molar concentration(2 and 4M),and the ratio of binder fineaggregate(1:3,1:4,and 1:5).Under these conditions,the specimens were tested using the compressive strength test,water absorption test,microstructure analysis,a freezing–thawing test and toxicity characteristic leaching procedure *** optimal formulation was composed of 1:4 binder fine-aggregate ratio,4M NaOH concentration,and 100-kgf/cm2 ***,we used a WGW and achieved a compressive strength of 50.6 MPa after 28 days,which was greater than 32 MPa and conformed to the Grade A brick standard of National Standards of the Republic of China(13295).In conclusion,this brick fabrication method based on geopolymer technology was not only beneficial to the environment but also improved the efficiency of reutilizing WGW.

关键词： Geopolymer waste grinding wheel geopolymer bricks

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

思想政治教育数字化的价值意蕴及实践进路

新疆师范大学学报（哲学社会科学版） 2025年第2期46卷 128-136页

作者：于祥成刘成合肥工业大学安徽合肥230009 武汉大学马克思主义学院湖北武汉430064

随着数字技术的广泛应用和教育数字化转型发展,数字化变革成为思想政治教育创新发展的必然选择。面对新的时代境遇,要全面把握思想政治教育数字化的内涵和特征,深刻认识思想政治教育数字化在推动思想政治教育认知重建、资源重组、话语... 详细信息

随着数字技术的广泛应用和教育数字化转型发展,数字化变革成为思想政治教育创新发展的必然选择。面对新的时代境遇,要全面把握思想政治教育数字化的内涵和特征,深刻认识思想政治教育数字化在推动思想政治教育认知重建、资源重组、话语重构、形态重塑等方面的重要价值。同时,要秉承数字理念,构建思想政治教育数字范式;搭建数字平台,优化思想政治教育数字服务;培育数字素养,提升思想政治教育数字技能;厚植“数字土壤”,形塑思想政治教育数字生态,积极探索思想政治教育数字化实践创新路径。

关键词：思想政治教育数字化数字技术实践进路

Spontaneously bundled nanotubes exhibit greatly enhanced emission via inter-nanotube energy transfer

在线全文

学校读者我要写书评

暂无评论

Science China chemistry 2016年第10期59卷 1348-1351页

作者： cheng Peng Juan Yang Yifan Zhang Yibin Zhang Yanke che Beijing National Laboratory for Molecular Sciences Key Laboratory of PhotochemistryInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Institute of Applied chemistry Henan Polytechnic UniversityJiaozuo 454003China

Spontaneously bundled nanotubes assembled from chiral perylene diimide molecules exhibit greatly enhanced emission via inter-nanotube excitation energy transfer. The formation of densely packed superstructures is an effective approach for high fluorescence quantum yield in assembled nanomaterials without compromising optoelectronic properties.

关键词： nanotube fluorescence quantum yield inter-tube energy transfer

Two novel rhodamine-perylenediimide fluorescent probes:Synthesis, photophysical properties, and cell imaging

在线全文

学校读者我要写书评

暂无评论

Chinese chemical Letters 2016年第6期27卷 879-886页

作者： Huan-Ren cheng ying Qian School of chemistry and chemical Engineering Southeast University

In this study, two novel dual-switch fluorescent chemosensors based on rhodamine-peryleneiimide have been designed and synthesized. The dual-switching behaviors of the sensors were based on the structural transformations of rhodamine and an intramolecular photoinduced electron transfer（PET）process from rhodamine to perylenediimide. These probes exhibited excellent sensitivity to protons with enhanced fluorescence emission from 500 nm to 580 nm. The fluorescence changes of probes were reversible within a wide range of p H values from 2.0 to 11.0. Moreover, the sensors exhibited high selectivity, short response time, and long lifetime toward protons. The possible mechanism was investigated by the DFT calculation and-1H NMR. According to the experiment of confocal laser scanning microscopy, these probes could be used to detect the acidic p H variations in living *** the Fundamental Research Funds for the National Natural Science Foundation of China （No. 61178057） and for the Central Universities （No. CXLX12_0085）. The characteristic data of compounds were in the Supplementary information.

关键词： Perylenediimide Rhodamine Photoinduced electron transfer Dual-switch