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Molecular cloning and expression of Pfu DNA polymerase gene and its application in long-distance PCR

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Chinese Science Bulletin 1998年第10期43卷 863-867页

作者： Bing Li cheng Gu Jindong zhao Peking Univ Coll Life Sci Beijing 100871 Peoples R China

A 2.3 kb DNA fragment containing Pfu DNA polA gene was amplified by PCR from total DNA of Pyrococcus furiosus and cloned into a pGEM-T vector. The recombinant clone pT-pfu was digested with Nco I and Xho I and the fragment was inserted into an expression vector pET3d-X. The Pfu polA gene was expressed in Escherichia coli BL21 (DE3). The gene product (Pfu) was purified with heat denaturation, polyethylenemine (PEI) precipitation and Bio-rex 70 ion-exchange chromatography. The recombinant Pfu was verified by protein N-terminal sequencing. With the recombinant Pfu, large λ DNA fragments were successfully amplified in long-distance PCR.

关键词： Pfu enzyme expression long-distance PCR.

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HEV用MH-Ni电池的研制

电源技术 2009年第3期33卷 197-199页

作者：张俊英王兴贺赵程王大伟张俊石张学增刘俊海天津和平海湾电源集团有限公司天津300384

报道了HEV用高功率MH-Ni电池的研制。通过优化设计单体及模块电池结构,优选正负极活性物质材料、基体材料及配方,提高了电池比功率,使之达到1100W/kg;改进正极配方,提高电池高温充电效率:60℃下0.5C充放电,效率达到90%;改进负极材料及配... 详细信息

报道了HEV用高功率MH-Ni电池的研制。通过优化设计单体及模块电池结构,优选正负极活性物质材料、基体材料及配方,提高了电池比功率,使之达到1100W/kg;改进正极配方,提高电池高温充电效率:60℃下0.5C充放电,效率达到90%;改进负极材料及配方,提高电池低温放电性能:-25℃1C放电达到初始容量的90%。

关键词： HEV MH-Ni电池高比功率

一种快速同步检测水稻黑条矮缩病毒和南方水稻黑条矮缩病毒的方法

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中国水稻科学 2011年第1期25卷 91-94页

作者：季英华高瑞珍张野程兆榜周彤范永坚周益军江苏省农业科学院植物保护研究所江苏南京210014 扬州大学园艺与植物保护学院江苏扬州225009

水稻黑条矮缩病毒和南方水稻黑条矮缩病毒是近年来在水稻上危害日益严重的两种病毒,由于两者同属于呼肠孤病毒科斐济病毒属,在症状、粒体形状、传播介体及寄主等方面非常相似,由此也给诊断及鉴定造成了困难。针对这一问题,通过设计简并... 详细信息

水稻黑条矮缩病毒和南方水稻黑条矮缩病毒是近年来在水稻上危害日益严重的两种病毒,由于两者同属于呼肠孤病毒科斐济病毒属,在症状、粒体形状、传播介体及寄主等方面非常相似,由此也给诊断及鉴定造成了困难。针对这一问题,通过设计简并引物,并优化体系,建立了一种快速、简便、灵敏、特异的方法,为这两种病毒的检测及鉴别提供了方便。

关键词：水稻黑条矮缩病毒南方水稻黑条矮缩病毒检测方法

Early expression of surfactant proteins D in Fusarium solani infected rat cornea

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International Journal of Ophthalmology(English edition) 2012年第3期5卷 297-300页

作者： cheng-Ye Che, Gui-Qiu zhao Department of Ophthalmology the Affiliated Hospital of Qingdao University Qingdao 266003 Shandong Province China Binzhou Hubin Eye Hospital Binzhou 256600 Shandong Province China

AIM: To investigate the early expression of surfactant proteins D(SP-D) in Fusarium solani infected rat cornea. METHODS: Wistar rats were divided into group A, B and C randomly. The right eyes were chosen as the experiment one. Group A was control group. Group B was not inoculated with Fusarium solani. Group C was taken as fusarium solani keratitis model. Five rats in group B and C were executed randomly at 6, 12, 24, 48 and 96 hours respectively after the experimental model being established. The expression of SP-D was assessed through immunohistochemistry and reverse transcription polyrnerase chain reaction(RT-PCR). RESULTS: RT-PCR detected that the SP-D mRNA expression was low in the corneal of normal rats and group B. The expression of fungal infected cornea increased gradually and reached the peak at 24 hours in group C. The synchronous expression of group B and C were in significant difference (P<0.01). Immunohistochemisty discovered the protein of SP-D expression was increased gradually from 12 hours and reached the peak at 48 hours in group C. The synchronous expression of group B and C were also in significant difference (P<0.01). CONCLUSION: There exists SP-D in rat corneal tissue and the expression is significantly increased at the early period of fusarium solani infected cornea. SP-D may play a role in the early innate immunity response of the corneal resistance to Fusarium solani infection.

关键词： keratitis Fusarium solani surfactant protein D innate immune

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白光LED的最新进展

发光学报 2000年第4期21卷 380-382页

作者：尹长安赵成久刘学彦侯凤勤郑岩卢景贵蒋大鹏申德振中国科学院激发态物理开放研究实验室

重点对近年来白光LED的进展进行评述。

关键词：白光发光二极管激发光谱显色指数视觉函数亮度色坐标

Microstructure and mechanical properties of spark plasma sintered Ti–Mo alloys for dental applications

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International Journal of Minerals,Metallurgy and Materials 2014年第5期21卷 479-486页

作者： Xin Lu Bo Sun Teng-fei zhao Lu-ning Wang cheng-cheng Liu Xuan-hui Qu State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing School of Materials Science and Engineering University of Science and Technology Beijing

Ti-Mo alloys with various Mo contents from 6wt% to 14wt% were processed by spark plasma sintering based on elemental pow- ders. The influence of sintering temperature and Mo content on the microstructure and mechanical properties of the resulting alloys were investigated. For each Mo concentration, the optimum sintering temperature was determined, resulting in a fully dense and uniform microstructure of the alloy. The optimized sintering temperature gradually increases in the range of 1100-1300℃ with the increase in Mo content. The microstructure of the Ti-（6-12）Mo ahoy consists of acicular α phase surrounded by equiaxed grains of 13 phase, while the Ti-14Mo al- loy only contains single 13 phase. A small amount of fine α lath precipitated from 13 phase contributes to the improvement in strength and hardness of the alloys. Under the sintering condition at 1250℃, the Ti-12Mo alloy is found to possess superior mechanical properties with the Vickers hardness of Hv 472, the compressive yield strength of 2182 MPa, the compression rate of 32.7%, and the elastic modulus of 72.1 GPa. These results demonstrate that Ti-Mo alloys fabricated via spark plasma sintering are indeed a perspective candidate alloy for dental applications.

关键词： titanium alloys spark plasma sintering microstructure mechanical properties

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三级医院输血科(血库)的规范化管理及方向

健康研究 2009年第4期29卷 274-276页

作者：洪慧东於王骐冯晓陈兆军杭州师范大学附属医院检验科浙江杭州310015

医院输血科(血库)是衔接供血与临床用血的重要桥梁,因此它的建设与管理至关重要。本院输血科根据卫生部颁布的相关法规,因时制宜,制定出一系列规范化的管理制度,保证了安全输血的有效实施。本文就其进行分析总结。

关键词：输血科规范化管理

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复吹转炉炼钢过程脱碳模型

钢铁研究 2006年第6期34卷 12-15,24页

作者：林东赵成林张贵玉彭飞邹宗树东北大学材料与冶金学院辽宁沈阳110004 本溪钢铁(集团)公司炼钢厂辽宁本溪117000

通过分析复吹转炉脱碳过程的冶金机理,应用冶金热力学、动力学和传输理论,建立描述转炉脱碳过程的动力学模型,确定了模型的有关参数。模型的关键在于正确计算并比较钢液中[C]和[O]的传质速度,找到转炉冶炼脱碳过程的限制环节,并通过一... 详细信息

通过分析复吹转炉脱碳过程的冶金机理,应用冶金热力学、动力学和传输理论,建立描述转炉脱碳过程的动力学模型,确定了模型的有关参数。模型的关键在于正确计算并比较钢液中[C]和[O]的传质速度,找到转炉冶炼脱碳过程的限制环节,并通过一系列热力学计算得到进入钢液的氧在各元素间的分配比例,从而得到转炉中脱碳反应的速度方程。将模型应用于本钢150 t转炉冶炼过程,模型在不同时刻的计算结果与现场实测值吻合较好。

关键词：复吹转炉炼钢传质速度氧分配比动力学模型

Mechanism of Li^(+)/Na^(+)separation by crown ether and butyrate acid root

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Rare Metals 2023年第4期42卷 1238-1248页

作者： Yong-Pan Tian cheng-cheng Wang Fan Zhang Sen Huang Liang Xu Zhuo zhao Bi-Hai Tong School of Metallurgical Engineering Anhui University of TechnologyMa’anshan243002China

Lithium is critical for economic growth since it is the primary component of ***^(+)is one of the main impurity ions in solution during the separation and enrichment of Li^(+).According to the size-matching effect between the cavities of crown ethers and Li+,crown ethers can selectively adsorb Li^(+).Herein,1,8-dihydroxyl-4,4,5,5-tetramethylbenzo-14-crown-4 was synthesized and used to extract lithium from a Li^(+)/Na^(+)mixed *** functional theory(DFT)was used to explore the properties of complexes with *** results show that the interactions between crown ethers and metal ions are due to electrostatic *** functional groups can synergistically extract Li^(+)/Na^(+)from solutions with the oxygen atom in the crown ether *** stability of the complex is also enhanced by van der Waals interactions between the butyrate acid root and crown ether.1,8-dihydroxyl-4,4,5,5-tetramethylbenzo-14-crown-4 has a stronger interaction with lithium butyrate than with sodium butyrate for most *** adsorption selectivity for Li+is proportional to the number of ether oxygen atoms that interact with Li^(+).The Li^(+)extraction efficiency increases from 3.93%to 20.93%in lithium hydroxide solution with the presence of butyrate acid *** the butyrate acid root is added to the mixed Li^(+)/Na^(+)solution,the Li^(+)extraction efficiency increases from 6.54%to 31.20%,while the Li^(+)/Na^(+)separation coefficient decreases from33.25 to 1.32.

关键词： Crown ether Selectivity Lithium Density functional theory(DFT) Electrostatic effect