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Journal of Environmental Sciences 2020年第4期32卷 59-66页

作者： Lihua Fan Xiaofeng Zhang Ruoxue Zeng Suhua Wang chenchen jin Yongqiang He Jiangbing Shuai Department of Food Science and Nutrition Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310016China Zhejiang Academy of Science and Technology for Inspection and Quarantine Hangzhou 310058China

To correctly assess and properly manage the public health risks associated with exposure to contaminated water,it is necessary to identify the source of fecal pollution in a watershed.In this study,we evaluated the efficacy of our two previously developed real time-quantitative PCR(qPCR)assays for the detection of swine-associated Bacteroidales genetic markers(gene 1-38,gene 3-53)in the Yangtze Delta watershed of southeastern China.The results indicated that the gene 1-38 and 3-53 markers exhibited high accuracy(92.5%,91.7%conditional probability,respectively)in detecting Bacteroidales spp.in water samples.According to binary logistic regression(BLR),these two swine-associated markers were well correlated(P<0.05)with fecal indicators(Escherichia coli and Enterococci spp.)and zoonotic pathogens(E.coli O157:H7,Salmonella spp.and Campylobacter spp.)in water samples.In contrast,concentrations of conventional fecal indicator bacteria(FIB)were not correlated with zoonotic pathogens,suggesting that they are noneffective at detecting fecal pollution events.Collectively,the results obtained in this study demonstrated that a swinetargeted qPCR assay based on two Bacteroidales genes markers(gene 1-38,gene 3-53)could be a useful tool in determining the swine-associated impacts of fecal contamination in a watershed.

关键词： Bacteroidales genes markers Water pollution Real-time quantitative PCR Fecal indicators Zoonotic pathogens

产黄曲霉毒素真菌双重荧光定量PCR检测技术的建立与应用

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中国粮油学报 2024年第2期39卷 188-195页

作者：陈冠果李可陆金虎金晨晨王龑帅江冰鲍维琪程昌勇宋厚辉张晓峰浙江农林大学动物科技学院·动物医学院浙江省畜禽绿色生态健康养殖应用技术研究重点实验室动物健康互联网检测技术浙江省工程研究中心浙江省动物医学与健康管理国际科技合作基地中澳动物健康大数据分析联合实验室杭州311300 浙江省检验检疫科学技术研究院杭州310016 浙江工业大学食品科学与工程学院杭州310014

为实现产黄曲霉毒素真菌的快速检测,本研究建立了检测产黄曲霉毒素真菌的aflR基因和nor-1基因的双重荧光定量PCR方法。分别针对aflR基因和nor-l基因保守序列,各设计2对特异性引物和探针,通过引物对间的有效性实验各筛选出1对最优的aflR... 详细信息

为实现产黄曲霉毒素真菌的快速检测,本研究建立了检测产黄曲霉毒素真菌的aflR基因和nor-1基因的双重荧光定量PCR方法。分别针对aflR基因和nor-l基因保守序列,各设计2对特异性引物和探针,通过引物对间的有效性实验各筛选出1对最优的aflR引物探针和nor-1引物探针,随后建立并优化双重荧光定量PCR的反应条件,构建标准曲线,测定该方法的重复性、灵敏度和特异性。结果显示,成功筛选出aflR-2和nor1-2 2组引物/探针组合,并优化和确定了引物/探针的最佳反应终浓度。特异性实验结果显示,本研究建立的检测方法仅对产黄曲霉毒素真菌的aflR基因和nor-1基因呈现特异性扩增,对黑曲霉、碳黑曲霉、赭曲霉和镰刀菌等常见产毒真菌的核酸样品均无扩增曲线。建立的方法重复性好,批内和批间变异系数均<3%,双重反应体系的检测灵敏度均低至1×10^(2)copies/μL。利用本研究所建立的方法与ELISA试剂盒分别对80份食品样品进行检测,结果显示,双重荧光定量PCR共检测出9份阳性样品,而ELISA试剂盒只检测出了7份阳性,表明本研究建立的方法具有更高的灵敏度和准确性。

关键词：产黄曲霉毒素真菌双重荧光定量PCR 快速检测

An integrated portable bio-monitoring system based on tough hydrogels for comprehensive detection of physiological activities

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Nano Research 2024年第1期17卷 321-332页

作者： Congcong Yang chenchen Ji Fengjiao Guo Chunjiang jin Hongyu Mi Zhongchang Wang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources School of Chemical Engineering and TechnologyXinjiang UniversityUrumqi 830017China International Iberian Nanotechnology Laboratory(INL) Avenide Mestre Jose VeigaBraga 4715-330Portugal

Advanced soft ion-conducting hydrogels have been developed rapidly in the integrated portable health monitoring equipment due to their higher sensitivity,sensory traits,tunable conductivity,and stretchability for physiological activities and personal healthcare detection.However,traditional hydrogel conductors are normally susceptible to large deformation and strong mechanical stress,which leads to inferior electro-mechanical stability for real application scenarios.Herein,a strong ionically conductive hydrogel(poly(vinyl alcohol)-boric acid-glycerol/sodium alginate-calcium chloride/electrolyte ions(PBG/SC/EI))was designed by engineering the covalently and ionically crosslinked networks followed by the salting-out effect to further enhance the mechanical strength and ionic conductivity of the hydrogel.Owing to the collective effects of the energy-dissipation mechanism and salting-out effect,the designed PBG/SC/EI with excellent structural integrity and robustness exhibits exceptional mechanical properties(elongation at break for 559.1%and tensile strength of 869.4 kPa)and high ionic conductivity(1.618 S·m^(-1)).As such,the PBG/SC/EI strain sensor features high sensitivity(gauge factor=2.29),which can effectively monitor various kinds of human motions(joint motions,facial micro-expression,faint respiration,and voice recognition).Meanwhile,the hydrogel-based Zn||MnO_(2)battery delivers a high capacity of 267.2 mAh·g^(-1)and a maximal energy density of 356.8 Wh·kg^(-1)associated with good cycle performance of 71.8%capacity retention after 8000 cycles.Additionally,an integrated bio-monitoring system with the sensor and Zn||MnO_(2)battery can accurately identify diverse physiological activities in a real-time and non-invasive way.This work presents a feasible strategy for designing high-performance conductive hydrogels for highly-reliable integrated bio-monitoring systems with excellent practicability.

关键词： Ionically conductive hydrogel strain sensor Zn-based battery integrated bio-monitoring system human motion monitoring

3D interlocked all-textile structured triboelectric pressure sensor for accurately measuring epidermal pulse waves in amphibious environments

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Nano Research 2024年第3期17卷 1923-1932页

作者： Shaobo Si chenchen Sun Yufen Wu jingjing Li Han Wang Yinggang Lin jin Yang Zhong Lin Wang Department of Optoelectronic Engineering Key Laboratory of Optoelectronic Technology and Systems Ministry of EducationChongqing UniversityChongqing 400044China College of Physics and Electronic Engineering Chongqing Normal UniversityChongqing 401331China Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 100083China School of Materials Science and Engineering Georgia Institute of TechnologyAtlantaGA 30332USA

The performance degradation and even damage of the e-textiles caused by sweat,water,or submersion during all-weather health monitoring are the main reasons that e-textiles have not been commercialized and routinized so far.Herein,we developed an amphibious,high-performance,air-permeable,and comfortable all-textile triboelectric sensor for continuous and precise measurement of epidermal pulse waves during full-day activities.Based on the principle of preparing gas by acid-base neutralization reaction,a one-piece preparation process of amphibious conductive yarn(ACY)with densely porous structures is proposed.An innovative three-dimensional(3D)interlocking fabric knitted from ACYs(0.6 mm in diameter)and polytetrafluoroethylene yarns exhibit high sensitivity(0.433 V·kPa^(-1)),wide bandwidth(up to 10 Hz),and stability(>30,000 cycles).With these benefits,98.8%agreement was achieved between wrist pulse waves acquired by the sensor and a high-precision laser vibrometer.Furthermore,the polytetrafluoroethylene yarn with good compression resilience provides sufficient mechanical support for the contact separation of the ACYs.Meanwhile,the unique skeletonized design of the 3D interlocking structure can effectively relieve the water pressure on the sensor surface to obtain stable and accurate pulse waves(underwater depth of 5 cm).This achievement represents an important step in improving the practicality of e-textiles and early diagnosis of cardiovascular diseases.

关键词： triboelectric pressure sensor amphibious fabric interlocking fabric pulse wave measurement one-piece preparation method

Simultaneous detection of Nosema bombycis,nucleopolyhedrovirus and densovirus by multiplex real-time PCR in Bombyx mori

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Simultaneous detection of Nosema bombycis,nucleopolyhedrovir...

第六届中国兽药大会

作者： Yongqiang He Shan Wu Xingmeng Lu Jiangbing Shuai Hongfei Mo Ruoxue Zen chenchen jin Huizhen Yu Zhejiang Entry-Exit Inspection and Quarantine Bureau Institute of Sericulture and Apiculture Zhejiang University

An effective,multiplex real-time PCR assay for the simultaneous detection of three major pathogens,Nosema bombycis(BmNb),nucleopolyhedrovirus(NPV) and densovirus(DNV),in B.mori was developed in this s

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水基多碳链醇自润湿流体界面特性研究

化工新型材料 2023年第1期51卷 178-184页

作者：靳辰辰邢美波张洪发王瑞祥北京建筑大学环境与能源工程学院北京市建筑能源高效综合利用工程技术研究中心北京100044

自润湿流体自发润湿高温端的特性在强化传热领域具有广阔的应用前景。对水基多碳链醇自润湿流体的气-液界面特性和固-液界面特性进行研究。选择随着碳原子数增加的正丁醇、正戊醇和正庚醇,制备了不同浓度的自润湿流体。并对紫铜表面进... 详细信息

自润湿流体自发润湿高温端的特性在强化传热领域具有广阔的应用前景。对水基多碳链醇自润湿流体的气-液界面特性和固-液界面特性进行研究。选择随着碳原子数增加的正丁醇、正戊醇和正庚醇,制备了不同浓度的自润湿流体。并对紫铜表面进行了不同加工方式的抛光打磨来研究自润湿流体的界面特性。结果表明:随着温度升高,高碳醇水溶液的表面张力先减小后增大,呈抛物线型。并且能够显著地降低水溶液的表面张力,在20~80℃范围内,7%丁醇水溶液表面张力平均降低了58%,2%戊醇水溶液平均降低了47%,0.1%庚醇水溶液平均降低了33%。固-液界面特性研究结果表明,在丁醇浓度<5%时,接触角随粗糙度的变化规律符合Wenzel模型预期。而当丁醇浓度≥5%时,Wenzel模型不能正确反映接触角随粗糙度的变化规律,且随着浓度的增加误差越大。此外,单向打磨紫铜表面,液滴出现形变现象。表现出明显的各向异性润湿特性,导致平槽接触角小于垂槽接触角。总之,随着水基多碳链醇自润湿流体浓度的增大和碳原子数的增加,其润湿性越好。

关键词：自润湿流体表面张力吸附特性粗糙度润湿性

从纳米水化硅酸钙到水泥净浆弹性性能多尺度递推模型

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材料导报 2024年第7期38卷 126-133页

作者：童涛涛李宗利刘士达张晨晨金鹏西北农林科技大学水利与建筑工程学院陕西杨凌712100 西北农林科技大学旱区农业水土工程教育部重点实验室陕西杨凌712100 山东电力工程咨询院有限公司济南250013

混凝土具有多尺度结构特征,其力学性能受到不同水化产物组分及微观结构的影响。基于分子动力学方法、化学计量法和均质化方法,建立了从纳米尺度水化硅酸钙(C-S-H)到水泥净浆弹性性能多尺度递推模型,其计算结果与实验数据吻合较好。基于... 详细信息

混凝土具有多尺度结构特征,其力学性能受到不同水化产物组分及微观结构的影响。基于分子动力学方法、化学计量法和均质化方法,建立了从纳米尺度水化硅酸钙(C-S-H)到水泥净浆弹性性能多尺度递推模型,其计算结果与实验数据吻合较好。基于该模型的计算结果可得:C-S-H凝胶中44%的孔隙率致使其体积模量、剪切模量和杨氏模量分别降低了约66%、53%和55%。当水灰比从0.3变化至0.5时,水泥净浆的体积模量、剪切模量和杨氏模量分别降低了约39%、30%和32%;LD C-S-H和毛细孔的体积分数分别增大了13%和20%。水化产物中C-S-H的体积分数越大,或水泥熟料中硅酸三钙的质量分数越大,水泥净浆的弹性参数越大。该模型为水泥基材料微观调控提供了指导。

关键词：纳米水化硅酸钙水泥净浆弹性性能多尺度递推模型分子动力学均质化

A discovery of two slow pulsars with FAST:“Ronin”from the globular cluster M15

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Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 166-180页

作者： Dengke Zhou Pei Wang Di Li Jianhua Fang chenchen Miao Paulo CCFreire Lei Zhang Dandan Zhang Huaxi Chen Yi Feng Yifan Xiao jintao Xie Xu Zhang Chenwu jin Han Wang Yinan Ke Xuerong Guo Rushuang Zhao Chenhui Niu Weiwei Zhu Mengyao Xue Yabiao Wang Jiafu Wu Zhenye Gan Zhongyi Sun Chengjie Wang Jie Zhang Junshuo Zhang jinhuang Cao Wanjin Lu Research Center for Astronomical Computing Zhejiang LaboratoryHangzhou 311121China National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Institute for Frontiers in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China University of Chinese Academy of Sciences Beijing 100049China New Cornerstone Science Laboratory Shenzhen 518054China Max Planck Institut f¨ur Radioastronomie Bonn 53121Germany Centre for Astrophysics and Supercomputing Swinburne University of TechnologyHawthorn 3122Australia School of Mathematical Sciences School of Physics and Electronic SciencesGuizhou Normal UniversityGuiyang 550001China Guizhou Provincial Key Laboratory of Radio Astronomy and Data Processing Guiyang 550001China School of Physics and Electronic Engineering Qilu Normal UniversityJinan 250200China School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550001China College of Physical Science and Technology Central China Normal UniversityWuhan 430079China Tencent Youtu Lab Shanghai 201103China

Globular clusters harbor numerous millisecond pulsars,but long-period pulsars(P 100 ms)are rarely found.In this study,we employed a fast folding algorithm to analyze observational data from multiple globular clusters obtained by the Five-hundredmeter Aperture Spherical radio Telescope(FAST),aiming to detect the existence of long-period pulsars.We estimated the impact of the median filtering algorithm in eliminating red noise on the minimum detectable flux density(S_(min))of pulsars.Subsequently,we successfully discovered two isolated long-period pulsars in M15 with periods approximately equal to 1.928451 and3.960716 s,respectively.On the P-˙P diagram,both pulsars are positioned below the spin-up line,suggesting a possible history of partial recycling in X-ray binary systems disrupted by dynamical encounters later on.According to timing results,these two pulsars exhibit remarkably strong magnetic fields.If the magnetic fields were weakened during the accretion process,then a short duration of accretion might explain the strong magnetic fields of these pulsars.

关键词： pulsar globular cluster FAST

网络嵌入对企业绿色创新的影响与作用机制:吸收能力的中介作用

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科技进步与对策 2021年第5期38卷 79-86页

作者：周礼金晨晨浙江工业大学社会科学研究院浙江工业大学管理学院浙江杭州310023

基于企业资源观与能力观,构建以潜在吸收能力与现实吸收能力为中介变量的链式中介模型,利用264份制造业企业调查问卷,探讨网络嵌入对企业绿色创新的影响与作用机制。研究发现:网络嵌入正向影响企业绿色创新;潜在吸收能力、现实吸收能力... 详细信息

基于企业资源观与能力观,构建以潜在吸收能力与现实吸收能力为中介变量的链式中介模型,利用264份制造业企业调查问卷,探讨网络嵌入对企业绿色创新的影响与作用机制。研究发现:网络嵌入正向影响企业绿色创新;潜在吸收能力、现实吸收能力分别在网络嵌入与企业绿色创新间起部分中介作用,并共同在网络嵌入与企业绿色创新间发挥链式中介作用。该研究拓展了网络嵌入影响企业绿色创新的内在机制,揭示了外部网络资源与内部动态能力在实现企业绿色创新中的重要性。

关键词：网络嵌入绿色创新潜在吸收能力现实吸收能力

坤泰胶囊联合唑吡坦治疗肾虚肝郁型更年期综合征睡眠障碍的疗效观察

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现代药物与临床 2024年第1期39卷 111-115页

作者：陈晨晨陈瑾姜海珍王云飞济宁医学院附属医院妇科山东济宁272029

目的探讨坤泰胶囊联合唑吡坦治疗肾虚肝郁型更年期综合征睡眠障碍的临床效果。方法选取2022年1月—2022年12月济宁医学院附属医院收治的120例肾虚肝郁型更年期综合征睡眠障碍患者,随机分为对照组与治疗组,每组各60例。对照组患者睡前口... 详细信息

目的探讨坤泰胶囊联合唑吡坦治疗肾虚肝郁型更年期综合征睡眠障碍的临床效果。方法选取2022年1月—2022年12月济宁医学院附属医院收治的120例肾虚肝郁型更年期综合征睡眠障碍患者,随机分为对照组与治疗组,每组各60例。对照组患者睡前口服酒石酸唑吡坦片,起始剂量5 mg/次,1次/d,最大剂量10 mg。治疗组在对照组基础上口服坤泰胶囊,4粒/次,3次/d。两组患者治疗12周。观察两组患者临床疗效,比较治疗前后两组患者中医证候积分和匹兹堡睡眠质量指数量表(PSQI)评分,及雌二醇(E_(2))、卵泡刺激素(FSH)和促黄体生成素(LH)水平。结果治疗后,治疗组患者总有效率较对照组显著升高(98.33%vs 88.33%,P<0.05)。治疗后,两组患者中医证候积分及PSQI评分均显著低于治疗前(P<0.05),且治疗组评分显著低于对照组(P<0.05)。治疗后,两组患者FSH和LH水平显著低于治疗前,而E_(2)水平显著高于治疗前(P<0.05),且治疗组患者性激素水平显著好于对照组(P<0.05)。结论坤泰胶囊联合唑吡坦治疗肾虚肝郁型更年期综合征睡眠障碍能有效调控患者性激素水平,改善中医证候及提高睡眠质量。

关键词：坤泰胶囊酒石酸唑吡坦片肾虚肝郁型更年期综合征睡眠障碍匹兹堡睡眠质量指数量表卵泡刺激素促黄体生成素