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Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

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Acta Oceanologica Sinica 2003年第2期22卷 243-254页

作者： chenyueqin ShaoPeng WangNing ZhouHui QuLianghu LindaK.Medlin KeyLaboratoryofGeneEngineeringofEducationMinistry BiotechnologyReserachCenterZhongshanUniversityGuangzhou510275China AlfredWegenerInstituteforPolarandMarineResearch Postfach120161Columbusstrasse27525BremerhavenGermany

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5. 8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that *** was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P. globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P. globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P. globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P .globosa to many areas, meaning that P. globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P. globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.

关键词： Genetic variation Phaeocystis globosa Phaeocystis pouchetii population dispersal rDNA ITS

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血小板电泳时间与脑电地形图相关性探讨

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现代电生理学杂志 1996年第3期3卷 111-112页

作者：黄惠军智屹忠陈月琴南京大学医学院附属南京鼓楼医院 210008 南京市脑科医院

本文分析了138例不同血小板电泳时间老年受检者的BEAM之间的关系。显示患者BEAM阳性率随血小板电泳时间延长呈正相关性增高。

关键词：脑电地形图血小板电泳时间

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