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维普期刊数据库博看期刊

BODIPY-derived ratiometric fluorescent sensors: pH-regulated aggregation-induced emission and imaging application in cellular acidification triggered by crystalline silica exposure

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Science China Chemistry 2018年第11期61卷 1413-1422页

作者： Yang Bai Danyang Liu Zhong Han Yuncong chen zhongyan chen Yang Jiao Weijiang He Zijian Guo State Key Laboratory of Coordination Chemistry Coordination Chemistry Institute School of Chemistry and Chemical EngineeringNanjing University

Modification of classic fluorophore to possess the emission transitions between aggregation-induced emission （ALE） and intrinsic emission offers reliable approach to the design of ratiometric fluorescent sensors. In this study, a proton acceptor benzimidazole was integrated with BODIPY to form three compounds, BBI-1/2/3, which demonstrated the AIE （-595 nm, Iagg） in neutral aqueous medium and intrinsic BODIPY emission （-510 nm,Iint） in acidic medium. All the three showed the ratiometric pH sensing behavior in a dual excitation/dual emission mode, yet BBI-3 displayed still the dual emission ratiometric pH sensing ability. The pH-dependent emission ratio/int/Iagg of the three were fully reversible, and no interference was observed from normal abundant chemical species in live cells. Their different pKa （BBI-1, pKa 4.4; BBI-2, pKa 2.7; BBI-3, pKa 3.6） suggested that the substituents on benzimidazole moiety were essential to govern their functioning pH range. The ratiometric imaging of BBI-1 in A549 cells provided an effective intracellular pH （pHi） calibration formula corresponding to emission ratio oflJ Iint/Iagg. Ratiometric pH imaging in A549 cells upon small particle exposure confirmed the particle-induced cellular acidification with this formula. Both particle size and the chemical nature of the particle contribute to the observed acidification effect. The synchronization of lysosome disruption to cellular acidification in A549 cells upon crystalline silica exposure was directly observed for the first time with BBI-1, showing the potential application of BBI-1 in the study of silicosis and other related diseases. This study de- monstrated that endowing fluorophore with AIE/intrinsic emission transition could be a promising strategy for ratiometric sensor design.

关键词： fluorescence imaging sensor cellular acidification aggregation-induced emission crystalline silica lysosome BODIPY

Distribution and Forming Model of Fluvial Terrace in the Huangshui Catchment and its Tectonic Indication

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Acta Geologica Sinica(English Edition) 2010年第2期84卷 415-423页

作者： WANG Xianyan LU Huayu Jef VANDENBERGHE chen zhongyan LI Langpin Geographic and Oceanographic Sciences Nanjing University Nanjing 210093 China Institute of Earth Sciences VU University Amsterdam De Bpoelelaan 1085 1081 HVAmsterdam The Netherlands Department of Geography Qinghai Normal University Xining 830008 China

The Huang Shui River, a main tributary of the Yellow River, crosses a series of tectonically subsided and uplifted areas that show different patterns of terrace formation. The distribution of fluvial terrace of the Huang Shui River is studied through topographic and sedimentologic terrace mapping. Three terraces in the Haiyan Basin, four terraces in the Huangyuan Basin, 19 terraces in the Xi＇ning Basin （the four high terraces may belong to another river）, nine terraces in the Ping＇an Basin, five terraces in the Ledu Basin and 12 terraces in the Minhe Basin are recognized. Sedimentology research shows that the geomorphologic and sedimentological pattern of the Huang Shui River, which is located at the margin of Tibet, are different from that of the rivers at other regions. The formation process of the terrace is more complicated at the Huang Shui catchment： both accumulation terrace and erosion terrace were formed in each basin and accumulation terraces were developed in some basins when erosion terraces were formed in other basins, indicating fluvial aggradation may occur in some basins simultaneously with river incision in other basins. A conceptual model of the formation process of these two kinds of fluvial terraces at Huang Shui catchment is brought forward in this paper. First, the equilibrium state of the river is broken because of climatic change and/or tectonic movement, and the river incises in all basins in the whole catchment until reaching a new equilibrium state. Then, the downstream basin subsides quickly and the equilibrium state is broken again, and the river incises at upstream basins while the river accumulates at the subsidence basin quickly until approaching a new equilibrium state again. Finally, the river incises in the whole catchment because of climatic change and/or tectonic movement and the accumulation terrace is formed at the subsidence basin while the erosion terrace is formed at other basins. The existence of the accumulation terrace im

关键词： Huangshui catchment fluvial terrace tectonic subsidence geomorphologic evolution late Cenozoic

味觉事件诱发电位仪的研制和味觉事件诱发电位测试验证

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中国中西医结合耳鼻咽喉科杂志 2024年第2期32卷 143-148页

作者：周小林陈忠岩李春景刘剑锋中日友好医院耳鼻咽喉头颈外科北京100029 北京协和医学院北京育达医疗科技股份有限公司

目的自主研制味觉刺激器,在此基础上组建味觉事件诱发电位仪,并使用该装置进行味觉事件诱发电位测试,以验证该系统的的可行性、特异性和可靠性。方法自主研制电动味觉刺激器,该刺激器主要包括味剂输送系统,可编程控制器、人机界面和... 详细信息

目的自主研制味觉刺激器,在此基础上组建味觉事件诱发电位仪,并使用该装置进行味觉事件诱发电位测试,以验证该系统的的可行性、特异性和可靠性。方法自主研制电动味觉刺激器,该刺激器主要包括味剂输送系统,可编程控制器、人机界面和味剂释放的口器。该刺激器能精准释放味觉刺激。用Neuro-Audio脑电平台作为脑电记录和分析系统。招募20名健康受试者进行味觉事件诱发电位测试。测试包括使用无味的蒸馏水和不同浓度的盐水(0.3%和0.6%)作为咸味刺激测试,另外在局部麻醉状态下进行咸味刺激。刺激17次进行叠加,记录电极为Cz和Fz。结果使用蒸馏水作为刺激剂时记录不到脑电波形,应用盐水(0.3%和0.6%)作为咸味刺激剂时均能记录到相应的脑电波形。典型的波形包括P1、N1、P2和N2波。而且在舌部表面麻醉后上述波形消失。另外随着咸味刺激浓度增加Cz和Fz记录到的P1、P2潜伏期明显缩短,差异具有统计学意义。结论本团队自主研发的味觉刺激器结合脑电记录系统能成功记录到特异性味觉事件诱发电位。

关键词：味觉事件诱发电位味觉味觉刺激器特异性

A ratiometric fluorescent sensor for tracking Cu(Ⅰ) fluctuation in endoplasmic reticulum

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Science China Chemistry 2019年第4期62卷 465-474页

作者： Jungu Guo Hao Yuan Yuncong chen zhongyan chen Menglong Zhao Liang Zou Yi Liu Zhipeng Liu Qiang Zhao Zijian Guo Weijiang He State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing University Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)Nanjing University of Posts & Telecommunications Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University (NanjingTech)

A two-photon ratiometric fluorescent sensor for Cu^+ in endoplasmic reticulum(ER), CNSB, was developed via coumarin/ASBD integration based on FRET mechanism. In solution, CNSB shows reversible, highly-specific ratiometric response to Cu^+ .Moreover, CNSB exhibits suitable K_d value, suggesting the possibility of detecting Cu^+ in the living cells. The probe can enter the MCF-7 cells easily and specifically locates in the ER. The highly specific ratiometric response of CNSB toward Cu^+ in MCF-7 cells provides the sensor the capacity to visualize both exogenous and endogenous Cu^+ in the ER via fluorescence ***, CNSB was utilized to detect the fluctuation and distribution of Cu^+ under ER stress in MCF-7 cells, which confirmed directly the relationship between Cu^+ enhancement and ER stress. Meanwhile, the two-photon ability of coumarin facilitated the sensor to visualize Cu^+ fluctuation via two-photon fluorescence imaging. In addition, the spatial distribution of Cu^+ in the heart slice of the 14-day-old rat was demonstrated using CNSB. This study demonstrates the promising potential of CNSB in clarifying the Cu^+ -dependent signaling in the ER stress-related diseases.

关键词： Cu(Ⅰ) ratiometric two-photon endoplasmic reticulum-targeting endoplasmic reticulum stress heart tissue slice

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新能源装载机远程驾驶控制器开发

内燃机与动力装置 2024年第4期41卷 71-76页

作者：岳远航纪少波刘振革胡珑渝陈忠言张强山东大学能源与动力工程学院山东济南250061 汽车动力系统智能检测及高端装备山东省工程研究中心山东济南250357 山东技师学院智慧交通学院山东济南250204 山东氢探新能源科技有限公司山东济南250100

为提高某混合动力装载机远程操作性能,采用32位高性能单片机开发控制器,基于5G通信技术和Simulink软件,设计开发装载机行走系统、液压系统、制动系统、转向系统及附件系统的控制策略,并通过时延、行走制动、作业测试进行试验验证,实现... 详细信息

为提高某混合动力装载机远程操作性能,采用32位高性能单片机开发控制器,基于5G通信技术和Simulink软件,设计开发装载机行走系统、液压系统、制动系统、转向系统及附件系统的控制策略,并通过时延、行走制动、作业测试进行试验验证,实现对装载机的远程驾驶控制。测试结果表明:整车端与远程端之间数据传输完整,数据传输时延约为25 ms;随着踩下远程端制动踏板,大约经过0.3 s整车端电机转矩从100 N·m降为0,大约经过0.7 s电机转速从800 r/min降为0;装载机摇臂、动臂均能根据远程先导手柄的控制指令正确反应,但动作存在轻微滞后;远程驾驶控制逻辑设计符合远程驾驶要求,满足实际使用需要。

关键词：新能源装载机远程驾驶控制策略功能测试

维普期刊数据库博看期刊

Discovery and characterization of a new hydrothermal field at 2°N on the slow-spreading Carlsberg Ridge

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Journal of Oceanology and Limnology 2024年第4期42卷 1106-1118页

作者： zhongyan QIU Yejian WANG Xiqiu HAN Honglin LI Xing YU Ruyong CUI Mou LI Xuegang chen Jiqiang LIU Key Laboratory of Submarine Geosciences&Second Institute of Oceanography Ministry of Natural ResourcesHangzhou 310012China Qingdao Institute of Marine Geology China Geological SurveyQingdao 266071China School of Earth Science Zhejiang UniversityHangzhou 310027China Ocean College Zhejiang UniversityZhoushan 316021China

A new hydrothermal field(Tianshi)was discovered on the rift valley wall through plume anomaly surveys and geological work conducted in 2012 and 2018 between 2°35′N and 2°43′N of the slow-spreading Carlsberg Ridge(CR).Here,the results of two expeditions conducted to detect and characterize the new hydrothermal field are *** and geochemical data,as well as 14 C ages of a sediment core collected near the field are presented to reveal the hydrothermal *** show that the Tianshi field is a basalt-hosted hydrothermal *** data of the sediments collected near the field indicate a strong hydrothermal contribution,and hydrothermal Fe and Cu fluxes range from 30 to 155 mg/(cm^(2)·ka)and 0.59 to 11.49 mg/(cm^(2)·ka),*** variations in the fluxes of hydrothermal Fe indicate that there have been at least three amplified hydrothermal venting events(H 1,H 2,and H 3)in the Tianshi field over the last 35.2 ka,in 28.6-35.2 ka BP,22.0-27.6 ka BP,and 1.2-11.4 ka BP,*** event H 2 was driven by an increased magmatic production associated with sea level fall during the Last Glacial Maximum,while event H 3 was promoted by tectonic activity associated with a rapid sea level *** study further verified the role of sea level change in modulating hydrothermal activity on mid-ocean ridges.

关键词： plume anomaly sediment core new hydrothermal field hydrothermal history Carlsberg Ridge

“双循环”格局下边疆经济体系现代化提升路径研究

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云南民族大学学报（哲学社会科学版） 2021年第3期38卷 121-127页

作者：陈忠言云南大学政府管理学院云南昆明650091

经济体系现代化与新格局的建设无论在目标和内容上都相辅相成、互为支撑。边疆地区因其独特的区位优势和资源禀赋在维护国家经济安全、扩大对外开放、连接国内国际市场、促进区域协调发展方面有着重要作用。本文在剖析了高质量标准下经... 详细信息

经济体系现代化与新格局的建设无论在目标和内容上都相辅相成、互为支撑。边疆地区因其独特的区位优势和资源禀赋在维护国家经济安全、扩大对外开放、连接国内国际市场、促进区域协调发展方面有着重要作用。本文在剖析了高质量标准下经济体系现代化内涵的基础上,从稳定性、效益性、创新性、绿色性、均衡性、开放性六个维度分析了边疆地区经济体系发展情况。边疆经济体系现代化的建设必须采取有针对性的措施提升其治理效能,促进边疆地区经济社会高质量的发展。

关键词：双循环边疆治理经济体系现代化

A Standard Time Resolution Detecting System for TOF-PET

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近代物理研究所和兰州重离子加速器实验室年报：英文版 2008年第1期 31-32页

作者： chen Jinda Xu Hushan Guo zhongyan chen Ruofu Hu Zhenguo Tang Bin Yue Ke Zhang Xueheng Yu Yuhong

多维贫困视角下滇西、乌蒙片区少数民族脱贫绩效对比

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维普期刊数据库

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经济问题探索 2020年第7期 147-156页

作者：陈忠言杨晗贾鼎云南大学政府管理学院昆明650091 中国科学院健康大数据研究中心北京100101 中国人民公安大学北京100038

少数民族贫困问题仍是制约我国2020年脱贫目标的重要难题,是农村精准扶贫工作的主战场,也是2020年以后相对贫困治理的重点。多维贫困研究方法关注贫困人群的全面发展,能为建立解决相对贫困的长效机制提供理论视角和操作途径。本项研究... 详细信息

少数民族贫困问题仍是制约我国2020年脱贫目标的重要难题,是农村精准扶贫工作的主战场,也是2020年以后相对贫困治理的重点。多维贫困研究方法关注贫困人群的全面发展,能为建立解决相对贫困的长效机制提供理论视角和操作途径。本项研究根据多维贫困理论和现阶段精准扶贫政策为依据设定了评价指标,并采用国务院扶贫办监测系统的数据,对比了滇西、乌蒙片区两个典型地区的多维贫困状况。研究发现外部地理因素对少数民族的多维贫困影响较为显著;贫困程度和户主的教育水平负相关;滇西片区对少数民族脱贫干预政策有效度很高。

关键词：多维贫困少数民族长效机制相对贫困