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Transcatheter Aortic Valve Replacement in Patients With Pure Native Aortic Regurgitation:Results From a Multicenter Registry Study

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Cardiology Discovery 2024年第2期4卷 134-141页

作者： Xiaofei Gao Juan Zhang Xiangquan Kong Jing chen Xiang chen Longyan Zhang Xinyong Cai Jiancheng Zhui Nailiang Tian Zhen Ge Bin Wang Qing Zhou Xi Su Lang Hong Yan Wang Hong Jiang Junjie Zhang shaoliang chen Department of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210000China Department of Cardiology Renmin Hospital of Wuhan UniversityWuhan UniversityWuhanHubei 430000China Department of Cardiology Xiamen Cardiovascular HospitalXiamen UniversityXiamenFujian 361000China Department of Cardiology Wuhan Asian Heart HospitalWuhanHubei 430000China Department of Cardiology Jiangxi Provincial People's HospitalNanchang UniversityJiangxi 330000China

Objective:Patients with untreated severe aortic regurgitation(AR)have a high risk of *** transcatheter aortic valve replacement(TF-TAVR)is a treatment option for AR;however,the safety and efficacy of this technique have not been sufficiently *** study aimed to evaluate the clinical and anatomical variables correlating with device success of TF-TAVR using a self-expanding valve system for pure ***:Patients with pure native severe AR who underwent TF-TAVR using a self-expanding valve system were registered at 5 Chinese *** primary endpoint was device success at 1 month after *** secondary endpoint was the composite of major adverse cardiovascular events(MACE)at 6 months,including all-cause death,ischemic stroke,emergency conversion to cardiac surgery,and permanent pacemaker *** was used to analyze the left ventricular function before the TAVR procedure and during *** logistic regression and Cox regression analyses were performed to find relevant independent risk ***:Between September 2019 and February 2022,79 patients with AR were enrolled in the *** 1 month,device success was achieved in 60(75.9%)*** 6 months,29(36.7%)patients had *** revealed improved left ventricular function after *** regression analysis demonstrated that the Society of Thoracic Surgeons risk score(odds ratio 0.760,95%confidence interval(Cl):0.584-0.989;P=0.041)and annulus perimeter(odds ratio 0.888,95%Cl:0.796-0.992;P=0.035)were 2 predictors of device ***,annulus perimeter(<80.2mm),but not Society of Thoracic Surgeons risk score,was associated with a significant reduction in MACE at 6 months(hazard ratio 2.223,95%Cl:1.060-4.659;P=0.028).Conclusions:TF-TAVR using a self-expanding valve system appears to be a safe and feasible treatment for patients with pure native severe AR,particularly those with a less enlarged annulus.

关键词： Aortic valve Aortic regurgitation Transcatheter aortic valve replacement Device success Self-expanding valve

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GRK2-YAP signaling is implicated in pulmonary arterial hypertension development

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Chinese Medical Journal 2024年第7期137卷 846-858页

作者： Peng Ye Yunfei Deng Yue Gu Pengfei Liu Jie Luo Jiangqin Pu Jingyu chen Yu Huang Nanping Wang Yong Ji shaoliang chen Division of Cardiovascular Molecular Laboratory Third Clinical CollegeNanjing Medical UniversityNanjingJiangsu 210006China Division of Cardiovascular Laboratory Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China Division of Cardiology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200080China Division of Pulmonary Surgery Wuxi People’s HospitalNanjing Medical UniversityWuxiJiangsu 300247China Institute of Vascular Medicine The Chinese University of Hong KongHongkong 999077China Health Science Center East China Normal UniversityShanghai 200241China School of Pharmacy Nanjing Medical UniversityNanjingJiangsu 210004China Division of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China

Background:Pulmonary arterial hypertension(PAH)is characterized by excessive proliferation of small pulmonary arterial vascular smooth muscle cells(PASMCs),endothelial dysfunction,and extracellular matrix remodeling.G protein-coupled receptor kinase 2(GRK2)plays an important role in the maintenance of vascular tone and blood ***,the role of GRK2 in the pathogenesis of PAH is ***:GRK2 levels were detected in lung tissues from healthy people and PAH patients.C57BL/6 mice,vascular smooth muscle cell-specific Grk2-knockout mice(Grk2^(∆SM22)),and littermate controls(Grk2^(flox/flox))were grouped into control and hypoxia mice(n=8).Pulmonary hypertension(PH)was induced by exposure to chronic hypoxia(10%)combined with injection of the SU5416(cHx/SU).The expression levels of GRK2 and Yes-associated protein(YAP)in pulmonary arteries and PASMCs were detected by Western blotting and immunofluorescence *** mRNA expression levels of Grk2 and Yes-associated protein(YAP)in PASMCs were quantified with real-time polymerase chain reaction(RT-PCR).Wound-healing assay,3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay,and 5-Ethynyl-2′-deoxyuridine(EdU)staining were performed to evaluate the proliferation and migration of ***,the interaction among proteins was detected by immunoprecipitation ***:The expression levels of GRK2 were upregulated in the pulmonary arteries of patients with PAH and the lungs of PH ***,cHx/SU-induced PH was attenuated in Grk2^(∆SM22) mice compared with littermate *** amelioration of PH in Grk2^(∆SM22) mice was accompanied by reduced pulmonary vascular *** vitro study further confirmed that GRK2 knock-down significantly altered hypoxia-induced PASMCs proliferation and migration,whereas this effect was severely intensified by overexpression of *** also identified that GRK2 promoted YAP expression and nuclear translocation in PASMCs,resulting in excessive PA

关键词： Pulmonary arterial hypertension GRK2 YAP Pulmonary artery smooth muscle cell Hypoxia

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Thirty-day Outcomes of First-in-man Implantation of a Novel Transcatheter Edge-to-edge Mitral Repair System in Patients With Severe Mitral Regurgitation

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Cardiology Discovery 2024年第2期4卷 142-147页

作者： Kai Xu Yan Wang shaoliang chen Xiangbin Pan Ben He Ruiyan Zhang Bin Wang Junjie Zhang Zhengbin Zhu Bin Wang Da Zhu Yaling Han Department of Cardiology General Hospital of Northern Theater CommandShenyangLiaoning 110016China Department of Cardiology Xiamen Cardiovascular Hospital Xiamen UniversityXiamenFujan 361102China Department of Cardiology Nanjing First HospitalNanjingJiangsu 210006China Department of Structure Heart Disease Fuwai Yunnan Cardiovascular HospitalKunmingYunnan 650102China Department of Cardiology Shanghai Chest HospitalShanghai 200030China Department of Cardiology Rujin Hospital Shanghai Jiaotong University School of MedicineShanghai 200025China

Objectives:The aim of this multicenter,prospective,single-arm pilot study(*** number:NCT05040074)was to observe the procedural and 30-day results of the novel transcatheter mitral valve repair system,SQ-Kyrin■-M Clip(Shenqi Medical,Shanghai,China),in patients with severe mitral regurgitation(MR).Methods:The heart team considered patients from 5 centers in China with clinically significant functional mitral regurgitation≥3+despite optimal medical therapy or degenerative mitral regurgitation≥3+with high surgical risk as candidates for transcatheter *** patients received transcatheter edge-to-edge mitral valve repair under general *** primary outcome was technical success,which included all of the following measured at the exit from the catheterization laboratory:(1)absence of procedural mortality;(2)successful access,delivery,and retrieval of the device delivery system;(3)successful deployment and correct positioning of the frst intended device;and(4)no emergency surgery or reintervention related to the device or access *** secondary outcomes included all-cause mortality,serious adverse events,device success,and procedural success 30 d after the ***:From June 2021 to December 2021,18 patients were enrolled in this study with age(75.7±7.4)*** patients had MR 4+,while 3 had MR 3+.Technical success was achieved in all patients,including 6 degenerative mitral regurgitation and 12 functional mitral regurgitation *** was no all-cause mortality at 30 *** patient had single leaflet device attachment within 30 d,which was regarded as a serious adverse event,and the patient was successfully treated with reintervention by implanting another *** patient's transmitral gradient was 6 mmHg(>5 mmHg),with an effective orifice area of 2.57 cm^(2) after the *** patients had device success and procedural success at 30 d *** patients had MR 1+,3 had MR 2+,and only 1

关键词： Heart failure Mitral regurgitation Transcatheter mitral valve repair Transcatheter edge-to-edge repair

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Chinese Society of Cardiology guidelines on the diagnosis and treatment of adult fulminant myocarditis

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Science China(Life Sciences) 2024年第5期67卷 913-939页

作者： Chinese Society of Cardiology,Chinese Medical Association,Writing Group Jiangang Jiang Hongyang Shu Dao Wen Wang Rutai Hui chenze Li Xiao Ran Hong Wang Jing Zhang Shaoping Nie Guanglin Cui Dingcheng Xiang Qun Shao Shengyong Xu Discussion experts:Ning Zhou Yuming Li Wei Gao Yuguo chen Yuan Bian Guoping Wang Liming Xia Yan Wang Chunxia Zhao Zhiren Zhang Yuhua Zhao Jianan Wang shaoliang chen Hong Jiang Jing chen Xianjin Du Mao chen Yinxian Sun Sheng Li Hu Ding Xueping Ma Hesong Zeng Li Lin Shenghua Zhou Likun Ma Ling Tao Juan chen Yiwu Zhou Xiaomei Guo 不详 Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Fuwai Hospital National Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100037China Zhongnan Hospital of Wuhan University Wuhan 430062China Fuwai Huazhong Cardiovascular Hospital Zhengzhou 450003China Beijing Anzhen Hospital Capital Medical UniversityBeijing 100029China Guangzhou General Hospital of Guangzhou Military Command Guangzhou 510010China Harbin Medical University Cancer Hospital Harbin 150081China Union Hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Taida Hospital Tianjin 300457China Peking University Third Hospital Beijing 100191China Qilu Hospital of Shandong University Jinan 250012China Kanghua Hospital DongguanGuangzhou 523080China Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003China Nanjing First Hospital Nanjing Medical UniversityNanjing 210006China Renmin Hospital of Wuhan University Wuhan 430060Wuhan West China Hospital Sichuan UniversityChengdu 610044China First Hospital of China Medical University Shenyang 110002China General Hospital of Ningxia Medical University Yinchuan 750003China The Second Xiangya Hospital Central South UniversityChangsha 410012China The First Affiliated Hospital of USTC University of Science and Technology of ChinaHefei 230002China The First Affiliated Hospital of Air Force Medical University Xi’an 710032China Central Hospital of Wuhan City Wuhan 430014China Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

Fulminant myocarditis is an acute diffuse inflammatory disease of *** is characterized by acute onset,rapid progress and high risk of *** pathogenesis involves excessive immune activation of the innate immune system and formation of inflammatory *** to China’s practical experience,the adoption of the“life support-based comprehensive treatment regimen”(with mechanical circulation support and immunomodulation therapy as the core)can significantly improve the survival rate and long-term *** emphasis is placed on very early identification,very early diagnosis,very early prediction and very early treatment.

关键词： fulminant myocarditis guidelines life support-based comprehensive treatment regimen diagnosis treatment cytokine storm overactivation of immunity

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港口航道工程中钻孔灌注桩施工技术应用研究——以某港口航道工程为例

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现代工程科技 2024年第9期3卷 85-88页

作者：陈绍良中交第四航务工程局第二工程有限公司广东广州510220

由于港口航道工程施工现场特殊的地质、水文条件,其对地基承载力要求较高,因此此类项目的钻孔灌注桩施工技术难度较高。结合某港口航道工程实践,对钻孔灌注桩施工技术应用展开了研究,优化了钻孔灌注桩施工方案。项目采取正循环泥浆护壁... 详细信息

由于港口航道工程施工现场特殊的地质、水文条件,其对地基承载力要求较高,因此此类项目的钻孔灌注桩施工技术难度较高。结合某港口航道工程实践,对钻孔灌注桩施工技术应用展开了研究,优化了钻孔灌注桩施工方案。项目采取正循环泥浆护壁进行钻进,同时加深主桩孔深,严格控制泥浆质量,有效解决了钻孔灌注桩水下作业的钻孔和清孔难题。工程实践表明,在软弱地基进行港口航道工程施工时,钻孔灌注桩工艺可以有效提升工程场地整体承载力,保证桩基项目施工质量。

关键词：港口航道钻孔灌注桩施工技术

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Ca^(2+)-ATPase参与植物耐盐性调控的研究进展

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植物研究 2024年第5期44卷 641-654页

作者：马秀英李金克周晓阳陈少良济宁学院生命科学与工程学院曲阜273155 北京林木分子设计育种高精尖创新中心北京林业大学生物科学与技术学院北京100083 北京林业大学实验室与设备管理处北京100083

盐胁迫下细胞质Ca^(2+)浓度升高,细胞会激活Ca^(2+)调节的靶酶或者与Ca^(2+)高度亲和的受体蛋白,其中,与Ca^(2+)高度亲和的受体蛋白中,植物钙泵(Ca^(2+)-ATPase)是P型ATP酶,包含内质网Ca^(2+)-ATPases与质膜Ca^(2+)-ATPas‐es,通过主动运输将Ca^(2+)从细胞质转移到质外体或细胞器。大量研究表明,植物的耐盐性在很大程度上与其维持钙泵即Ca^(2+)-ATPase活性的能力有关。多种植物Ca^(2+)-ATPase对盐胁迫表现出敏感性,并受到外源Ca^(2+)的保护,表明外源钙处理与Ca^(2+)-ATPase活性可能在盐胁迫下的细胞内钙稳态和信号转导中起重要作用。该研究概述了植物Ca^(2+)-ATPase类型、结构与性质,亚细胞定位Ca^(2+)-ATPase及外源钙与亚细胞定位Ca^(2+)-ATPase参与植物耐盐调控研究进展,重点对质膜、液泡膜、核膜、内质网及高尔基体Ca^(2+)-ATPases参与植物耐盐调控的研究进展进行了综述,并提出展望。该研究为了解植物耐盐性生理及分子机制提供帮助,同时为作物耐盐栽培提供新思路。

关键词： Ca^(2+)-ATPase 质膜液泡膜内质网核膜盐胁迫

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胡杨PeMAX2调控拟南芥耐旱性

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北京林业大学学报 2024年第6期46卷 106-117页

作者：马思圆尧俊李静安珂悦赵瑞赵楠周晓阳陈少良北京林业大学生物科学与技术学院林木、花卉遗传育种教育部重点实验室北京100083 广东省森林培育与保护利用重点实验室广东省林业科学研究院广东广州510520 北京林业大学验室与设备管理处北京100083

【目的】MAX2能抑制植物的分枝,且是独角金内酯信号传导途径的关键调控因子。MAX2同时还参与多种激素间的交叉互作,在植物抵御生物和非生物胁迫中发挥至关重要的作用。前期研究发现胡杨PeMAX2能调控拟南芥的离子平衡和耐盐性,但对于PeM... 详细信息

【目的】MAX2能抑制植物的分枝,且是独角金内酯信号传导途径的关键调控因子。MAX2同时还参与多种激素间的交叉互作,在植物抵御生物和非生物胁迫中发挥至关重要的作用。前期研究发现胡杨PeMAX2能调控拟南芥的离子平衡和耐盐性,但对于PeMAX2的耐旱功能还知之甚少,本文将探索胡杨PeMAX2调控拟南芥耐旱性的作用机制。【方法】将胡杨PeMAX2在拟南芥中异源表达,进行渗透胁迫和干旱胁迫,研究过表达PeMAX2拟南芥的生理和分子响应机制。【结果】(1)在长期干旱胁迫下,胡杨叶片PeMAX2基因表达水平升高。(2)甘露醇处理后,PeMAX2转基因拟南芥的种子萌发率和根长均高于野生型拟南芥(WT)和max2突变体,并且转基因株系细胞膜受损较轻。渗透胁迫后,转基因株系超氧化歧化酶、过氧化物酶、过氧化氢酶活性及其编码基因上调表达的升高幅度均显著高于拟南芥WT和max2突变体,转基因株系调控根细胞H_(2)O_(2)水平的能力更强。(3)土壤干旱处理10 d后,转基因株系叶绿素含量下降幅度低于WT和max2突变体,而且过表达株系在干旱胁迫下能维持较高水平的PSⅡ最大光化学效率、相对电子传递效率和实际光合量子产量;在碳同化和气孔导度方面,转基因拟南芥叶片的净光合速率和气孔导度均高于WT和突变体,说明过表达PeMAX2提高了拟南芥转基因植株在干旱条件下光合作用的能力。土壤复水后WT和突变体叶绿素含量、荧光和光合作用的恢复程度也明显低于转基因株系。【结论】过表达胡杨PeMAX2可以提高拟南芥的耐旱性,主要是由于过表达Pe MAX2提高了转基因拟南芥活性氧平衡的调控能力,减弱了干旱胁迫对生物膜的氧化损伤和光合作用的抑制。

关键词：胡杨 PeMAX2 拟南芥耐旱性抗氧化酶光合作用

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过表达胡杨PePKS5提高拟南芥耐盐性

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北京林业大学学报 2024年第2期46卷 62-74页

作者：温馨殷可欣马思圆张小萌赵楠周晓阳赵瑞陈少良北京林业大学生物科学与技术学院林木、花卉遗传育种教育部重点实验室北京100083 北京林业大学实验室与设备管理处北京100083

【目的】盐胁迫是影响植物生长发育的不利环境因子。蛋白激酶PKS5作为植物盐超敏感信号转导途径的重要组分,在植物响应盐胁迫过程中具有重要调节功能。本文旨在生理水平和分子水平上,探究胡杨PePKS5基因在植物耐受盐胁迫过程中的调节作... 详细信息

【目的】盐胁迫是影响植物生长发育的不利环境因子。蛋白激酶PKS5作为植物盐超敏感信号转导途径的重要组分,在植物响应盐胁迫过程中具有重要调节功能。本文旨在生理水平和分子水平上,探究胡杨PePKS5基因在植物耐受盐胁迫过程中的调节作用。【方法】克隆胡杨PePKS5基因,并在拟南芥中过表达,获得T3代转基因拟南芥纯合体。观察盐胁迫下转基因拟南芥株系的耐盐表型,测定其过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶活性及盐胁迫响应基因的表达量。利用非损伤微测技术测定转基因拟南芥株系根尖Na^(+)、K^(+)动态离子流,利用激光共聚焦显微镜观测转基因拟南芥株系根尖Na^(+)和H_(2)O_(2)含量。测定盐处理后转基因拟南芥土培幼苗的叶绿素荧光参数、光合参数等生理指标,揭示PePKS5基因在盐胁迫下对拟南芥的生理调节作用。构建亚细胞定位载体,通过瞬时转化烟草,对PePKS5蛋白进行亚细胞定位观测。【结果】(1)PePKS5基因的CDS序列编码417个氨基酸,PePKS5氨基酸序列与毛白杨PtPKS5相似度最高,并且亲缘关系最近。(2)PePKS5定位于细胞质和细胞核中。(3)NaCl处理6 h后,胡杨叶片PePKS5基因表达量上调,72 h后逐渐恢复至正常水平。(4)盐胁迫下过表达PePKS5基因的拟南芥株系(PePKS5-OE10和PePKS5-OE15)的生存率和根长均明显高于野生型(WT)和转空载体(VC)株系。(5)与WT和VC株系相比,PePKS5-OE10和PePKS5-OE15株系的POD和CAT活性增强,APX1和CAT2基因的表达量均升高。(6)盐胁迫下PePKS5-OE10和PePKS5-OE15株系的Na^(+)外排和K^(+)的内流明显高于WT和VC株系,并且在根尖中积累的Na^(+)浓度和H_(2)O_(2)浓度明显低于WT和VC株系。(7)盐胁迫下PePKS5-OE10和PePKS5-OE15株系的叶绿素相对含量、PSⅡ最大光量子效率、实际光合量子产量和相对电子传递速率均高于WT和VC株系。(8)盐胁迫下PePKS5-OE10和PePKS5-OE15的净光合速率高于WT和VC株系,而胞间CO_(2)浓度、气孔导度和蒸腾速率均低于WT和VC株系。【结论】过表达胡杨PePKS5基因能够增强拟南芥对Na^(+)的外排能力,维持植物活性氧的平衡状态,以及保持相对稳定的光合作用能力,从而提高拟南芥的耐盐能力。

关键词：胡杨蛋白激酶盐胁迫 PKS5 离子平衡活性氧

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Dosimetry of chimeric TNT in lung tumor patients

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Nuclear Science and Techniques 2007年第4期18卷 227-231页

作者： chen Yangchun chen shaoliang JU Dianwen SHI Hongcheng YAO Zhifeng Nuclear Medicine Department Zhongshan Hospital Fudan University Shanghai200032 China PET/CT Center of the First Affiliated Hospital of Guangzhou Medical College Guangzhou 510230 China Shanghai MediPharm Biotech Co Ltd Shanghai 201203 China

The purpose of this study was to assess the absorbed dose of tumor and main critical organs in 131I labeled chimeric tumor necrotic treatment (chTNT). In 9 patients, a single intravenous dose of (29.6±3.7) MBq/kg was administered. Blood samples were drawn at different time intervals, and urine was collected for up to one week. Tissue distribution of 131I-chTNT was followed for up to one week by gamma camera imaging. Absorbed doses to the whole body and to normal organs were computed according to the MIRD scheme using Mirdose-3 software. S-factors for lung tumors were estimated by comparison with lungs of similar mass and position in the body. It was found that mean serum disappearance half time values for 131I-chTNT were (4.93±9.36) h and (61.7±21.2) h for α, β respectively, while that for whole body was(99±10) h. Mean urine biological clearance half time value was (90±10) h. The absorbed dose to tumor was (8.28±2.65) Gy, and the tumor-to-nontumor dose ratio was 3.95±1.55. And the mean effective dose to patients was (1.02±0.29) mSv/MBq.

关键词：肺癌放射疗法放射量碘放射性同位素

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PERIODIC CARDINAL INTERPOLATORY WAVELETS

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Chinese Annals of Mathematics,Series B 1998年第2期19卷 133-142页

作者： chen H. Xiao S. Institute of Mathematics Academia Sinica Beijing 100080 China

The authors construct periodic interpolating wavelets and their duals from a periodic function g(x) whose Fourier coefficients are positive. The corresponding decomposition and reconstruction algorithm is also given. The spline example shows that such kind of wavelets shares good localization with any desired regularity and symmetry. The construction depends essentially on the finite Fourier Transformation and the theory of circulant matrix.

关键词： Periodic wavelet Multiresolution Cardinal Interpolation Dual basis Circulant matrix

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