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Gene Expression Patterns in Different Wool Densities of Rex Rabbit Using cDNA Microarray

Agricultural Sciences in China 2011年第4期10卷 595-601页

作者： chen sai-juan LIU Tao LIU Ya-juan DONG Bing GU Zi-lin College of Animal Science and Technology Agricultural University ofHebei Baoding 071001 P.R. China Ringpu （Baoding） Biological Pharmaceutical Co. Ltd. Baoding 071000 P.R. China Mountain Area Research Institute Agricultural University of Hebei Baoding 071001 P.R.China Division of Scientific Research Agricultural University of Hebei Baoding 071001 P.R.China

This study was carried out to identify gene expression patterns in different wool densities of rex rabbit. The mid-dorsal skin samples from 8 rex rabbits were studied. They were divided into two groups according to different wool densities. Total RNA was isolated and labeled by reverse transcription reaction with Cy5-dCTP and Cy3-dCTP for cDNA probe. The cDNA probe was hybridized with cDNA microarrays containing 14 601 rabbit’s genes. The differently expressed genes were analysed with the Gene Ontology （GO） classification and the pathway analysis. Hierarchical clustering was performed to clarify genes in association with different wool densities. The 2 657 differentially expressed genes were identified. Among them, 1 103 genes were functionally known genes, 687 genes were up-regulated and 419 were down-regulated. GO analysis indicated that these altered genes were associated with metabolism, signal transduction, cell cycle, cell adhesion, cell proliferation, cell division, apoptosis, and other processes. KEGG analysis showed that 95 signal pathways associated with up-regulated genes and 87 signal pathways associated with down-regulated genes had changed significantly （P0.05）. Some important differentially expressed genes in different wool densities of rex rabbit were identified, such as MMP2, TGF-β1, TGF-β2, IGF-1, ITGB1, RPS6KB1, BMP2, ActRIIB, CDK2, and CCNA2. Hierarchical clustering analysis separated the differentially expressed genes into 5 main characteristic groups. Data from cDNA microarray experiments clearly distinguished between group A and group B. Some important genes were identified, which might be useful in further study on wool density markers of rex rabbit.

关键词： rex rabbit wool density gene expression cDNA microarray GO and KEGG analysis

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酶解豆粕对肉鸡生长性能、抗氧化能力、免疫性能和肠道屏障功能的影响

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饲料研究 2024年第6期47卷 36-42页

作者：范东峰梁慧情吴峰洋陈赛娟陈宝江魏忠华河北农业大学动物科技学院河北保定071001 河北省山区农业技术创新中心河北保定071001 秦皇岛启浩生物科技有限公司河北秦皇岛066003 河北省畜牧兽医研究所河北保定071000

试验旨在研究酶解豆粕对肉鸡生长性能、抗氧化能力、免疫性能和肠道屏障功能的影响。选取400只1日龄健康的科宝肉鸡,随机分为5个组,每组8个重复,每个重复10只。对照组饲喂基础饲粮,试验组(T1组、T2组、T3组和T4组)饲喂分别用1%、2%、3%... 详细信息

试验旨在研究酶解豆粕对肉鸡生长性能、抗氧化能力、免疫性能和肠道屏障功能的影响。选取400只1日龄健康的科宝肉鸡,随机分为5个组,每组8个重复,每个重复10只。对照组饲喂基础饲粮,试验组(T1组、T2组、T3组和T4组)饲喂分别用1%、2%、3%和4%酶解豆粕替代基础饲粮中等量豆粕的试验饲粮。试验期42 d。结果表明,与对照组相比,试验组肉鸡的平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)均无显著差异(P>0.05)。与对照组相比,T3组肉鸡回肠丙二醛(MDA)含量显著降低(P0.05)。与对照组相比,T2组和T4组肉鸡血清D-乳酸(D-LA)含量显著降低(P0.05)。研究表明,在肉鸡饲粮中添加酶解豆粕可以提高机体抗氧化能力和免疫性能,改善肠道屏障功能,使用3%的酶解豆粕替代肉鸡饲粮中等量的豆粕效果明显。

关键词：酶解豆粕肉鸡抗氧化能力免疫性能

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Exploration of the financing and management model of a children＇s critical disease security system in China based on the implementation of Shanghai Children Hospital Care Aid

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Chinese Medical Journal 2011年第6期124卷 947-950页

作者： ZHANG Zhi-ruo WEN Zhao-jun chen sai-juan chen Zhu Shanghai Jiao Tong University School of Public Health Shanghai 200025 China Shanghai Institute of Hematology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

This study is designed to serve as a reference for the establishment of health security systems for children＇s critical diseases. Through analysis of the operation of Shanghai Children Hospital Care Aid （SCHCA）, this study explored the financing model and management of a children＇s critical disease healthcare system and analyzed the possibility of expanding this system to other areas. It is found that a premium as Iow as RMB 7 per capita per year under SCHCA can provide high-level security for children＇s critical diseases. With the good experience in Shanghai and based on the current basic medical insurance system for urban residents and the new rural cooperative medical scheme （NRCMS）, it is necessary and feasible to build a health security system for children＇s critical diseases at the national level.

关键词： children critical diseases health security system

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Effects of seepage pressure on the mechanical behaviors and microstructure of sandstone

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第6期16卷 2033-2051页

作者： Xuewei Liu Juxiang chen Bin Liu sai Wang Quansheng Liu Jin Luo State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China Faculty of Engineering China University of GeosciencesWuhan430074China Key Laboratory of Safety for Geotechnical and Structural Engineering of Hubei Province School of Civil EngineeringWuhan UniversityWuhan430072China

Surrounding rocks of underground engineering are subjected to long-term seepage pressure,which can deteriorate the mechanical properties and cause serious *** order to understand the impact of seepage pressure on the mechanical property of sandstone,uniaxial compression tests,P-wave velocity measurements,and nuclear magnetic resonance(NMR)tests were conducted on saturated sandstone samples with varied seepage pressures(i.e.0 MPa,3 MPa,4 MPa,5 MPa,6 MPa,7 MPa).The results demonstrate that the mechanical parameters(uniaxial compressive strength,peak strain,elastic modulus,and brittleness index),total energy,elastic strain energy,as well as elastic strain energy ratio,decrease with increasing seepage pressure,while the dissipation energy and dissipation energy ratio ***,as seepage pressure increases,the micro-pores gradually transform into meso-pores and *** increases the cumulative porosity of sandstone and decreases P-wave *** numerical results indicate that as seepage pressure rises,the number of tensile cracks increases progressively,the angle range of microcracks is basically from 50-120to 80-100,and as a result,the failure mode transforms to the tensile-shear mixed failure ***,the effects of seepage pressure on mechanical properties were *** results show that decrease in the effective stress and cohesion under the action of seepage pressure could lead to deterioration of strength behaviors of sandstone.

关键词： Rock mechanics Mechanical property Seepage pressure Numerical simulation Microcracks

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Perception-Driven Learning of High-Dynamic Jumping Motions for Single-Legged Robots

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Journal of Bionic Engineering 2024年第4期21卷 1733-1746页

作者： Nengxiang Sun Fei Meng sai Gu Botao Liu Xuechao chen Zhangguo Yu Qiang Huang School of Mechatronical Engineering Beijing Institute of TechnologyBeijing100081China Key Laboratory of Biomimetic Robots and Systems Ministry of EducationBeijing100081China

Legged robots show great potential for high-dynamic motions in continuous interaction with the physical environment,yet achieving animal-like agility remains significant *** animals usually predict and plan their next locomotion by combining high-dimensional information from proprioception and exteroception,and adjust the stiffness of the body’s skeletal muscle system to adapt to the current *** control methods have limitations in handling high-dimensional state information or complex robot motion that are difficult to plan manually,and Deep Reinforcement Learning(DRL)algorithms provide new solutions to robot motioncontrol *** by biomimetics theory,we propose a perception-driven high-dynamic jump adaptive learning algorithm by combining DRL algorithms with Virtual Model Control(VMC)*** robot will be fully trained in simulation to explore its motion potential by learning the factors related to continuous jumping while knowing its real-time jumping *** policy trained in simulation is successfully deployed on the bio-inspired single-legged robot testing platform without further *** results show that the robot can achieve continuous and ideal vertical jumping motion through simple training.

关键词： Deep reinforcement learning High-dynamic jump Perception driven Single-legged robot

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Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity

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Signal Transduction and Targeted Therapy 2024年第1期9卷 259-270页

作者： Yan Sun Xiu-Na Yang Shuang-Shuang Yang Yi-Zhu Lyu Bing Zhang Kai-Wen Liu Na Li Jia-chen Cui Guang-Xiang Huang cheng-Lin Liu Jie Xu Jian-Qing Mi Zhu chen Xiao-Hu Fan sai-juan chen Shuo chen Shanghai Institute of Hematology National Research Center for Translational MedicineState Key Laboratory of Medical GenomicsRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Department of Hematology Second Hospital of Dalian Medical UniversityDalian 116023China National Facility for Protein Science Shanghai Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China Legend Biotech China Nanjing 211112China Shanghai Immune Therapy Institute Shanghai Cancer InstituteState Key Laboratory of Oncogenes and Related GenesRenji Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200127China Wondercel Biotechnology Shenzhen 518052China

Ligand-induced receptor dimerization or oligomerization is a widespread mechanism for ensuring communication specificity,safeguarding receptor activation,and facilitating amplification of signal transduction across the cellular ***,cell-surface antigeninduced multimerization(dubbed AIM herein)has not yet been consciously leveraged in chimeric antigen receptor(CAR)engineering for enriching T cell-based *** co-developed ciltacabtagene autoleucel(cilta-cel),whose CAR incorporates two B-cell maturation antigen(BCMA)-targeted nanobodies in tandem,for treating multiple *** we elucidated a structural and functional model in which BCMA-induced cilta-cel CAR multimerization amplifies myeloma-targeted T cell-mediated *** analysis of BCMA–nanobody complexes revealed atomic details of antigen–antibody hetero-multimerization whilst analytical ultracentrifugation and small-angle X-ray scattering characterized interdependent BCMA apposition and CAR juxtaposition in ***-induced nanobody CAR multimerization enhanced cytotoxicity,alongside elevated immune synapse formation and cytotoxicity-mediating cytokine release,towards myeloma-derived *** results provide a framework for contemplating the AIM approach in designing next-generation CARs.

关键词： cytotoxicity Antigen elevated

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NIR-IIb-triggered photodynamic therapy combined with chemotherapy platform based on rare-earth-doped nanoparticles

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Rare Metals 2024年第7期43卷 3220-3231页

作者： Zhuo Tu Yong Wan juan Ge chen-chen Li Tao Liang Zhen Li Chemistry and Chemical Engineering Hubei UniversityWuhan 430062China

Photodynamic therapy(PDT),with high spatiotemporal selectivity,minimal side effects and less drug resistance,has emerged as a promising approach for the treatment of ***,inadequate penetration depth and poor efficacy seriously restrict its practical *** address these challenges,we developed a combined therapy platform(RENP@C/D-FA)based on rare-earth-doped nanoparticles(RENPs)with a structure of NaErF_(4):Tm(0.5%)@NaYF_(4),on which photosensitizer Ce6(chlorin e6)and chemo-therapeutic drug(DOX)(doxorubicin)were loaded by encapsulating with an amphiphilic polymer distearoyl phosphatidylethanolamine-polyethylene glycol-folic acid(DSPE-PEG-FA).RENPs with NaErF_(4) as the matrix possess multiple excitation/emission peaks,enabling activation of PDT under near-infrared-Ⅱb(NIR-Ⅱb,1500-1700 nm)excitation and luminescence imaging in NIR-Ⅱb region by absorbing 808 nm near-infrared ***,DOX can be selectively released in the acidic microenvironment of *** from the tumor targeting capability of folic acid,this platform can successfully ablate tumor cells and inhibit tumor growth by combining PDT and *** innovative approach holds great promise as a powerful tool for cancer treatment,showing the potential to revolutionize PDT.

关键词： Photodynamic therapy Rare-earth-doped nanoparticles NIR-IIb Chemotherapy Combined therapy

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中药成分靶向治疗急性髓系白血病研究进展

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中国中西医结合杂志 2009年第1期29卷 14-18页

作者：镇涛陈赛娟上海交通大学医学院附属瑞金医院上海血液学研究所医学基因组国家重点实验室中国科学院上海生命科学研究院健康科学研究所上海200025

白血病是一类基因组发生动态变化的造血干/祖细胞疾病,以异常造血细胞的恶性增殖、分化受阻、凋亡受抑并造成正常血细胞减少为特征,白血病是一种常见的疾病,发病率在肿瘤疾病中居第6位。资料显示,全球每年新增的白血病患者30万人,而死... 详细信息

白血病是一类基因组发生动态变化的造血干/祖细胞疾病,以异常造血细胞的恶性增殖、分化受阻、凋亡受抑并造成正常血细胞减少为特征,白血病是一种常见的疾病,发病率在肿瘤疾病中居第6位。资料显示,全球每年新增的白血病患者30万人,而死于白血病者亦达22.2万人;我国每年新增白血病患者4万余人,发病率约为3－4/10万,并且还处于不断上升的趋势。[第一段]

关键词：急性髓系白血病靶向治疗中药成分白血病患者细胞疾病血细胞减少动态变化恶性增殖

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FIPIL1-PDGFRα alone or with other genetic abnormalities reveals disease progression in chronic eosinophilic leukemia but good response to imatinib

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Chinese Medical Journal 2008年第10期121卷 867-873页

作者： WANG Lin-na PAN Qin FU Jian-fei SHI Jing-yi JIN Jie LI Jun-ming HU Jion ZHAO Wei-li chen Zhu chen sai-juan State Key Laboratory of Medical Genomics Shanghai institute of Hematology Shanghai Ruijin Hospital Shanghai Jiao TongUniversity School Of Medicine Shanghai 200025 China Zhejiang Institute of Hematology First Affiliated HospitalZhejiang University School of Medicine Hangzhou Zhejiang 310003 China

Background The FIP1L1-PDGFRa fusion gene plays an important role in the pathogenesis of chronic eosinophilic leukemia （CEL） and is a direct therapeutic target of the tyrosine kinase inhibitor imatinib mesylate. Methods In 24 hypereosinophilic syndromes （HES） patients, using reverse transcriptase-polymerase chain reaction （RT-PCR）, nested PCR and sequence analysis, we investigated the frequency of FIPIL1-PDGFRa and other abnormalities of tyrosine kinase family genes like PDGFRa, PDGFRβ, C-KIT, FGFR1, ABL and FLT3 as well as gene mutation ＂hotspots＂, like MPL515 and JAK2V617F, frequently involved in myeloproliferative diseases. Fluorescence in situ hybridization was used to confirm the 4q 12 deletion. Results The FIP1L1-PDGFRa fusion transcript was found in 8 （33%） of 24 patients with HES, corresponding to the chromosome 4q12 deletion identified by FISH. The FIP1L 1-PDGFRα-associated patients diagnosed with CEL, frequently had hepatosplenomegaly, eosinophil-related tissue damage, anemia, thrombocytopenia, myelofibrosis and a short overall survival time. Nevertheless, imatinib mesylate induced rapid and complete hematological responses in treated FIP1L1-PDGFRa cases, followed by molecular remission and reversal of myelofibrosis. FIP1L1-PDGFRa fusion could co-exist with other mutations of tyrosine kinase family genes, like FLT3 or PDGFRβ. We also demonstrated that the SNPs of PDGFRβ were associated with selective splicing of exon 19 in case 20. Conclusions Correlating the CEL genotype with phenotype, FIP1L1-PDGFRa emerges as a relatively homogeneous clinicobiological entity that co-exists with other abnormalities of tyrosine kinase family genes. It reflects the disease progression and there is a good response to imatinib. Detection of the FIP1L 1-PDGFFla fusion gene is valid for both CEL diagnosis and therapy surveillance.

关键词： chronic eosinophilic leukemia FIP1L1-PDGFRa tyrosine kinase imatinib mesylate myeloproliferative disease

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Body-force modeling considering induced upstream effects for a transonic compressor with total temperature distortion

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Chinese Journal of Aeronautics 2024年第6期37卷 7-19页

作者： Jiahui QIU chen YANG Wenqiang ZHANG Min ZHANG juan DU Digital Twin Research Center Institute of Engineering ThermophysicsChinese Academy of SciencesBeijing 100190China School of Engineering and Science University of Chinese Academy of SciencesBeijing 100190China National Key Laboratory of Science and Technology on Advanced Light-duty Gas-turbine Chinese Academy of SciencesBeijing 100190China School of Mechatronical Engineering Beijing Institute of TechnologyBeijing 100081China

To numerically study the impact of total temperature distortion on a transonic compressor with reduced computational costs,a Body-Force Model(BFM)is ***,the interactions between the distorted flow and the compressor are analyzed using full-annulus Unsteady Reynolds-Averaged Navier-Stokes(URANS)results and the orbit *** is found that the induced swirl distortion and the mass flux nonuniformity are intensified in the compressor upstream flow field.A correction factor is thus added to the BFM to account for the effect of the induced swirl,which is crucial for the accurate representation of distortion transfer in the ***,steady simulations with large-amplitude 180circumferential total temperature distortion are performed using the developed *** is shown that the distorted compressor map simulated with the BFM matches well with URANS *** circumferential phase shift of total temperature and the generation of the additional total pressure distortion across the rotor are in line with the time-averaged URANS flow *** compressor upstream effects on the distorted inflow can also be exactly *** above-mentioned results demonstrate the BFM developed in this paper can effectively capture the distorted flow features inside the compressor,and significantly reduce the computational costs by five orders of magnitude compared with URANS.

关键词： Upstream effect Orbit method Induced swirl distortion Body force model Correction factor Time-averaged URANS

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