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A Study on the Activity of Carboxylesterase and the Differential Expression of Its Gene in the Midguts of Bombyx mori Resistant to BmDNV-Z

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Agricultural Sciences in China 2007年第8期6卷 1018-1026页

作者： GAO Gui-tian chen ke-ping YAO Qin chen Hui-qing WANG Lin-ling XU Jia-ping ZHAO Yuan WANG Yong-jie Institute of Life Sciences Jiangsu University Zhenjiang 212013 P.R.China Department of Food Enginneering Shaanxi Normal University Xi＇an 710062 P.R.China

This study was to discuss the relationship among the change in the activity of Bombyx mori carboxylesterase （BmCarE） in the midguts, the differential expression of BmCarE gene （bmcare） in the midguts, and the ability of Bombyx mori resistant to densonucleosis virus （BmDNV）, and to elucidate the molecular mechanism of resistance to BmDNV-Z. With two silkworm strains, HUABA, which is susceptible to BmDNV-Z, and BC8 （a near isogenic line of HUABA）, which is completely resistant to the same virus, as materials, the activity of BmCarE in the midgut was determined by Bio-Tek Synergy, and the differential expression of bmcare between the two strains was investigated by real-time fluorescence quantitative PCR, both at 12, 36, and 72 h post oral inoculation of the two strains with virus （hereafter referred as inoculation）. While the activity of BmCarE in the midguts of BC8 inoculation group at 12 h post inoculation was higher than that in the BC8 control group, the HUABA inoculated group, and the HUABA control group by 3.28, 2.26, and 3.02 times, respectively, with the difference being highly significant （P 〈 0.01）, there was no statistical difference among the other groups. The relative expression level of bmcare in the midguts of BC8 inoculation group at 12 h post inoculation was higher than that in the BC8 control group, the HUABA inoculation group, and the HUABA control group by 17.714, 21.76, and 15.09 times, respectively, with the difference being highly significant （P 〈 0.01）, and there was no statistical difference among other groups. The elevation of BmCarE activity and expression level of bmcare in the resistant strain at 12 h post inoculation may relate to the resistant gene （nsd/nsd） and the stimulation of BmDNV-Z. The molecular basis for the elevation of BmCarE activity in the resistant strain BC8 may be the change in the expression level of bmcare.

关键词： Bombyx mori resistance BmDNV-Z midgut carboxylesterase enzyme activity differential expression

Laparoscopic resection of synchronous intraductal papillary mucinous neoplasms:A case report

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World Journal of Gastroenterology 2012年第44期18卷 6510-6514页

作者： Xiao-Wu Xu Rong-Hua Li Wei Zhou Jie Wang Ren-Chao Zhang ke chen Yi-ping Mou Department of General Surgery Sir Run Run Shaw Hospital School of Medicine Zhejiang Uni- versity Hangzhou 310016 Zhejiang Province China

We describe herein a 68-year-old woman who was diagnosed with a quite rare entity of intraductal papillary mucinous neoplasms(IPMNs) occurring simultaneously in the left lateral lobe of liver and the tail of *** computed tomography and magnetic resonance cholangiopancreatography showed a cystic dilatation of the pancreatic duct in the pancreatic tail,which suggested an IPMN,and multiple intrahepatic duct stones in the left lateral *** patient underwent a laparoscopic left lateral hepatolobectomy and spleenpreserving distal ***-operative finding of massive mucin in the dilated bile duct implied an intraductal mucinous tumor in the *** diagnosis of synchronous IPMNs in the liver and pancreas was confirmed by pathological *** patient was followed up for 6 mo without signs of *** several cases of IPMN of liver without any pancreatic association have been reported,the simultaneous occurrence of IPMNs in the liver and pancreas is very *** the best of our knowledge,it is the first reported case treated by laparoscopic resection.

关键词： Laparoscopy Pancreas Liver Intraductalpapillary mucinous neoplasm Synchronous neoplasm

Prevalence of Atrial Fibrillation in China and Its Risk Factors

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Biomedical and Environmental Sciences 2013年第9期26卷 709-716页

作者： LI Ying WU Yang Feng chen ke ping LI Xian ZHANG Xing XIE Gao Qiang WANG Fang Zheng ZHANG Shu State key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Disease Chinese Academy of Medical Sciences and Peking Union Medical College Peking University Clinical Research Institute The George Institute for Global Health at Peking University Health Science Center

Objective To study the prevalence of atrial fibrillation （AF） and the relation with its risk factors in China. Methods A total of 19 363 participants （8635 males and 10 728 females） aged 〉35 years in geographically dispersed urban and rural regions of China were included in this cross-sectional survey. All participants received questionnaire, physical and blood examination. Echocardiography were performed for AF patients found in the survey. Results Of the 19 363 participants, 199 were diagnosed with AF. The estimated age-standardized prevalence of AF was 0.78% in men and 0.76% in women. The prevalence of AF in participants aged 〈60 years was 0.41% in men and 0.43% in women, and was 1.83% in both men and women aged 〉_60 years. About 19.0% of males and 30.9% of females with AF were diagnosed with valve disease. Age- and sex-adjusted multivariable logistic regression analysis revealed that myocardial infarction, left ventricular hypertrophy （LVH）, obesity, and alcohol consumption were associated with a increased risk of AF（P〈0.05）. Conclusion The age standardized prevalence of AF is 0.77% in the participants enrolled in the present study. The number of AF cases aged 〉35 years is 5.26 million according to 2010 Chinese Census. Most risk factors for AF, identified mainly in Western countries, are also detected in China.

关键词： Atrial fibrillation Epidemiology Risk factors Chinese population

Impact of automatic threshold capture on pulse generator longevity

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Chinese Medical Journal 2006年第11期119卷 925-929页

作者： chen Ruo-han chen ke-ping WANG Fang-zheng HUA Wei ZHANG Shu Centre of Arrhythmia Diagnosis and Treatment Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical College Beijing 100037 China

Background The automatic, threshold tracking, pacing algorithm developed by *** Medical, verifies ventricular capture beat by beat by recognizing the evoked response following each pacemaker stimulus. This function was assumed to be not only energy saving but safe. This study estimated the extension in longevity obtained by AutoCapture （AC） compared with pacemakers programmed to manually optimized, nominal output. Methods Thirty-four patients who received the St. Jude Affinity series pacemaker were included in the study. The following measurements were taken： stimulation and sensing threshold, impedance of leads, evoked response and polarization signals by 3501 programmer during followup, battery current and battery impedance under different conditions. For longevity comparison, ventricular output was programmed under three different conditions：（1） AC on; （2） AC off with nominal output, and （3） AC off with pacing output set at twice the pacing threshold with a minimum of 2.0 V. Patients were divided into two groups： chronic threshold is higher or lower than 1 V. The efficacy of AC was evaluated. Results Current drain in the AC on group, AC off with optimized programming or nominal output was （14.33±2.84） mA, （16.74±2.75） mA and （18.4±2.44） mA, respectively （AC on or AC off with optimized programming vs. nominal output, P〈0.01）. Estimated longevity was significantly extended by AC on when compared with nominal setting [（103±27） months, （80±24） months, P〈0.01）. Furthermore, compared with the optimized programming, AC extends the longevity when the pacing threshold is higher than 1 V. Conclusion AC could significantly prolong pacemaker longevity; especially in the patient with high pacing threshold.

关键词： autocapture current drain longevity pulse generator

Evaluation of NR2B peptide as subunit vaccines against experimental neuropathic pain

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Chinese Medical Journal 2007年第8期120卷 643-647页

作者： WANG Gong-ruing TIAN Yu-ke chen Jian-ping TIAN Xu-bi GAO Feng YANG Hui AN ke MA Guo-ping Department of Anaesthesiology Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Background NR2B containing N-methyI-D-aspartate （NMDA） receptor plays an important role in the facilitation and maintenance of neuropathic pain. The discrete distribution of NR2B subunit in the central nervous system （CNS） may support reduced side effects of agents that act selectively at this site. Therefore, we investigated the hypothesis that a humoral autoimmune response targeting the NR2B subunit of NMDA receptor relieves pain like behaviours produced by peripheral injury. Methods Rats were immunized subcutaneously with NR2B-keyhole Limpet Hemocyanin （NR2B-KLH） three times at two-week intervals. NR2B specific IgG titres in sera and cerebrospinal fluid were determined by indirect ELISA. Seven days after the third immunization, 2 of the 3 terminal branches of the sciatic nerve （tibial and common peroneal nerves） were tightly ligated. Behavioural testing was carried out on every other day after surgery, until 7 days after surgery. The lumbar spinal cord （L4-6） was removed on day 7 after ligation. The expression of NR2B protein in the lumbar spinal cord was determined using Western blotting. Results After the second vaccination, NR2B specific IgG in sera was detected to be 〉0.5 μg/ml in six of nine rats. After the third vaccination, all the immunized rats had 〉2.2 μg/ml. Titres of NR2B specific IgG in sera peaked 42 days post initial immunization and persisted for over 70 days. No NR2B specific IgG was detected in sera from PBS or KLH group. The behavioural thresholds in NR2B group were significantly higher than those in PBS and KLH groups on day 7 after ligation. NR2B specific IgG in CSF in NR2B group could not be detected on day 1 before spinal dissection; but could be detected on day 7 after surgery. The expression of NR2B protein in group NR2B was significantly lower than in PBS and KLH groups on day 7 after surgery. Conclusion The NR2B peptide could be used as a vaccine against neuropathic pain, which could be associated with reduction of NR2B protein in

关键词： peripheral neuropathic pain immunization NR2B peptide vaccine spared nerve injury

Prognostic factors affecting the all-cause death and sudden cardiac death rates of post myocardial infarction patients with low left ventricular ejection fraction

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Chinese Medical Journal 2009年第7期122卷 802-806页

作者： DAI Shi-mo ZHANG Shu chen ke-ping HUA Wei WANG Fang-zheng chen Xin Center for Arrhythmia Diagnosis and Treatment Fu Wai Hospitaland Cardiovascular Institute Chinese Academy of MedicalSciences & Peking Union Medical College Beijing 100037 China

Background Post myocardial infarction （post-MI） patients with low left ventricular ejection fraction （LVEF） have been candidates for an implantable cardioverter-defibrillator （ICD） since the Multicenter Automatic Defibrillator Implantation Trail Ⅱ （MADIT Ⅱ）. However, due to the high costs of ICDs, widespread usage has not been accepted. Therefore, further risk stratification for post-MI patients with low LVEF may aid in the selection of patients that will benefit most from ICD treatment. Methods Four hundred and seventeen post-MI patients with low LVEF （≤35%） were enrolled in the study. All the patients received standard examination and proper treatment and were followed up to observe the all-cause death rate and sudden cardiac death （SCD） rate. Then COX proportional-hazards regression model was used to investigate the clinical factors which affect the all-cause death rate and SCD rate. Results Of 55 patients who died during （32±24） months of follow-up, 37 （67%） died suddenly. After adjusting for baseline clinical characteristics, multivariate COX proportional-hazards regression model identified the following variables associated with death from all causes： New York Heart Association （NYHA） heart failure class ≥Ⅲ （Hazard ratio： 2.361）, LVEF ≤20% （Hazard ratio： 2.514）, sustained ventricular tachycardia （Hazard ratio： 6.453）, and age 〉70 years （Hazard ratio： 3.116）. The presence of sustained ventricular tachycardia （Hazard ratio： 6.491） and age ≥70 years （Hazard ratio： 2.694） were specifically associated with SCD. Conclusions In the post-MI patients with low LVEF, factors as LVEF ≤20%, age ≥70 years, presence of ventricular tachycardia, and NYHA heart failure class ≥111 predict an adverse outcome. The presence of sustained ventricular tachycardia and age ≥70 years was associated with occurrence of SCD in these patients.

关键词： myocardial infarction death, sudden, cardiac heart failure

Short-term effect of cardiac resynchronization therapy in patients with ischaemic or nonischaemic cardiomyopathy

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Chinese Medical Journal 2006年第18期119卷 1507-1510页

作者： HUA Wei NIU Hong-xia WANG Fang-zheng ZHANG Shu chen ke-ping chen Xin Department of Clinical Electrophysiology Fuwai Hospital and Cardiovascular Institute Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100037 China

Background Patients with heart failure were candidates for cardiac resynchronization therapy （CRT） regardless of underlying aetiology. This study observed the effect of CRT in patients with ischaemic or nonischaemic cardiomyopathy. Methods One hundred and forty-two patients with refractory chronic heart failure and left bundle branch block received cardiac resynchronization therapy, 91 men and 51 women, average age 60 years. Left ventricular ejection fraction （LVEF） was severely depressed （mean 29%）, left ventricular end diastolic diameter （LVEDD） enlarged （mean 72 mm） and QRS width was lengthened （mean 147 ms）. Ninety-eight had nonischaemic cardiomyopathy and 44 had ischaemic cardiomyopathy. Results After cardiac resynchronization therapy, the heart function was significantly improved. The mean LVEF increased from 29% to 36% after pacing. In patients with nonischaemic cardiomyopathy, the LVEF was improved from 28% to 37%, and in patients with ischaemic cardiomyopathy, the LVEF was improved from 30% to 36%. No significant difference of the improvement was found between the two groups （P〉0.05）. Conclusions Cardiac resynchronization therapy could significantly improve cardiac function in patients with chronic heart failure regardless of the underlying heart disease.

关键词： cardiac resynchronization therapy cardiomyopathy, ischaemic nonischaemic

Complications of cardiac resynchronization therapy in patients with congestive heart failure

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Chinese Medical Journal 2006年第6期119卷 449-453页

作者： NIU Hong-xia HUA Wei WANG Fang-zheng ZHANG Shu chen ke-ping chen Xin Centre of Arrhythmia Diagnosis and Treatment Fuwai HospitalChinese Academy of Medical Sciences and Peking Union MedicalCollege Beijing 100037 China

Background Previous clinical studies have suggested that patients with congestive heart failure and intraventricular conduction delay could benefit from cardiac resynchronization therapy （CRT）. Implantation of left ventricular lead is a complex procedure with some potential for complications. This study was conducted to analyse the complications of CRT in patients with congestive heart failure.Methods Totally 117 patients, 86 males and 31 females, mean age of 53 years, with congestive heart failure and intraventricular conduction delaywere enrolled in this study. Venography was performed on all patients. Different types of coronary sinus leads were used to pace the left ventricle.Results Left ventricular lead was attempted to implant through coronary sinus for all the 117 patients and was successfully implanted in 111 patients. The success rate was 94.9%. Main complications rate was 6.8%, including coronary sinus dissection in 4 patients, phrenic nerve stimulation required lead repositioning in 2 patients and lead dislodgement in 2 patients.Conclusions It is feasible and safe to pace left ventricle through coronary sinus. However, there are some procedural complications.

关键词： cardiac resynchronization therapy congestive heart failure compfication

Home monitoring system improves the detection of ventricular arrhythmia and inappropriate shock

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Chinese Medical Journal 2012年第19期125卷 3421-3424页

作者： WANG Huan HUA Wei DING Li-gang WANG Jing chen ke-ping ZHANG Shu Centre of Arrhythmia Diagnosis and Treatment Fuwai HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100037China

Background The impact of home monitoring system in the early detection of ventricular arrhythmia and inappropriate shock in daily work is not clear. The aim of this study was to investigate the impact of home monitoring system on the early detection of ventricular arrhythmia and inappropriate shock in daily clinical practice. Methods Cases of implantable cardioverter defibrillator （ICD） implantation with or without the home monitoring system from June 2010 to October 2011 at our center were reviewed. Follow-up was scheduled after implantation. Data relating to the home monitoring ICD were retrieved using a remote transmitter system. Data relating to the other devices were obtained during scheduled follow-up or unscheduled visits. Results Our study involved 69 patients （mean age （68.4＋17.6） years, 64.3% males, 26 in the home monitoring group vs. 43 in the non-home monitoring group）. In all, 561 ventricular arrhythmia episodes were detected in 17 patients （39.5%） in the non-home monitoring group： 495 episodes were ventricular tachycardia and 66 episodes were ventricular fibrillation; among these, 476 episodes of ventricular tachycardia and 45 episodes of ventricular fibrillation were appropriately diagnosed （96.1% and 68.2%, respectively）. In the home monitoring group, 389 ventricular arrhythmia episodes were transmitted by the home monitoring system in nine patients （34.6%）： 348 ventricular tachycardia episodes and 41 ventricular fibrillation episodes. Device detection was appropriate in 348 ventricular tachycardia episodes （100.0%） and 36 ventricular fibrillation episodes （87.8%）. The home monitoring group showed a higher appropriate detection rate of ventricular tachycardia （P 〈0.01） and ventricular fibrillation （P=0.02）. The proportion of inappropriate shock was comparable in the two groups （6/11 in the non-home monitoring group vs. 1/7 in the home monitoring group; m=0.08）. Conclusions The home monitoring ICD was able to provide information relating to inappropri

关键词： home monitoring system ventricular tachyarrhythmia implantable cardioverter defibrillator