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Energy requirements of Dorper×thin-tailed Han crossbred ewes during non-pregnancy and lactation

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Journal of Integrative Agriculture 2015年第12期14卷 2605-2617页

作者： LOU Can SI Bing-wen DENG Kai-dong MA Tao JIANG cheng-gang TU Yan ZHANG Nai-feng JI Shou-kun chen dan-dan DIAO Qi-yu Feed Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Feed Biotechnology Ministry of Agriculture College of Animal Science Jinling Institute of Technology

This experiment was conducted to investigate the energy requirement of Dorper×thin-tailed Han crossbred ewes during non-pregnancy and lactation. Fifteen ewes after parturition were randomly assigned to three treatments： ad libitum（100%） feed intake and 80 or 60% ad libitum intake, and another nine non-pregnant ewes were assigned to a blank control group. Digestibility trials were performed in the non-pregnant ewes and in the lactating ewes on the 20 th, 50 th, and 80 th d of lactation. In parallel with the digestibility trial, a respirometry experiment was conducted to determine the methane and carbon dioxide production with an open-circuit respirometry system that was equipped with respiratory chambers. The net energy（NE） and metabolizable energy（ME） requirements for maintenance and growth were calculated using the carbon and nitrogen balance method. The results revealed that the carbon（C） and nitrogen（N） excretions and energy losses at faeces and urine, as well as the output of methane and CO2, increased significantly with decreasing feed intake（P〈0.01）. The apparent digestibilities of C in th e stages of non-pregnancy and early, middle and late lactation were 55.8–58.3%, 62.5–73.8%, 64.8–71.3%, and 61.7–65.0%, respectively, and the apparent digestibilities of N were 45.2–51.3%, 73.7–82.7%, 72.8–80.5%, and 73.6–76.5%, respectively. The corresponding energy apparent digestibilities were 52.0–56.3%, 60.7–76.6%, 61.0–68.8%, and 61.4–67.7%, respectively. The ME/DE（digestible energy） values were 79.5–85.9%, 79.4–83.5%, 81.0%–85.3% and 78.6–82.9%, respectively. The maintenance requirements of NE, ME, and the efficiencies of ME utilisation for maintenance during the stages of non-pregnancy and early, middle and late lactation were 215.5, 253.1, 247.7, and 244.7 k J kg^（–1） BW0.75 d, and 372.4, 327.1, 320.9, and 362.0 k J kg^（–1） BW0.75 d, and 0.58, 0.77, 0.77, and 0.68, respectively. The ME requirement for the growth of non-pregnant ewes was 31.3 MJ kg^（–1） BW gain.

关键词： net energy metabolizable energy ewe lactation digestible energy

Exogenous p27KIP1 expression induces anti-tumour effects and inhibits the EGFR/PI3K/Akt signalling pathway in PC3 cells

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Asian Journal of Andrology 2009年第6期11卷 669-677页

作者： Jun chen dan Xia Jin-dan Luo Ping Wang Department of Urology The First Affiliated Hospital Medical College of ZheJiang University Hangzhou 310003 China

p27 is a cyclin-dependent kinase inhibitor that regulates the progression of cells from G1 to S phase of the cell cycle. Loss of p27 has been associated with disease progression and with an unfavourable outcome in prostate cancer. In this study, we investigated whether exogenous p27 expression in the human androgen-independent prostate cancer PC3 cell line had any effect on cell growth, and we studied the molecular mechanisms involved. p27 expression was restored in PC3 cells by plasmid delivery. Cell proliferation and apoptosis were assessed in PC3 cells transfected with p27. We also investigated the effects of p27 on the epidermal growth factor receptor （EGFR）/ phosphatidylinositol 3-kinase （PI3K）/Akt signalling pathway in PC3 cells. By restoring p27 expression in PC3 cells, we observed that p27 reduced proliferation and induced arrest in G0/G1 phase. Moreover, p27-transfected PC3 cells underwent apoptosis, as shown by flow cytometric analysis and western blotting analysis of Bcl-2, Bax, Bad, caspase-3 and poly（ADP-ribose）polymerase expression. Furthermore, the p27-induced anti-tumour action corre- lated with inhibition of the EGFR/PI3K/Akt signalling pathway, as confirmed by western blotting analysis and densitometry of EGFR, PI3K （p85）, Akt and p-Akts473 expression. Our results suggest that exogenous expression of p27 inhibits the proliferation of PC3 cells through induction of G1 arrest and apoptosis, and this process correlates with inhibition of the EGFR/PI3K/Akt signalling pathway.

关键词： apoptosis EGFR p27 prostate cancer cells signalling pathway

Unprotected Sex with Casual Partners: A Neglected Source of HIV Transmission among Members of the Yi Minority in Southwestern China

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Biomedical and Environmental Sciences 2014年第10期27卷 824-831页

作者： QIN Si LI A Sha MA Ming Ju LUO Qi WANG Xiao Lin NAN Lei ZENG Can XIAO Lin LI Qiao Qiao ZUO Jian Bing chen dan dan CUI Xin Yu LUAN Rong Sheng Department of Epidemiology West China School of Public Health Sichuan University Department of Preventive Medicine Medical School Hubei University for Nationalities Center for Disease Control and Prevention of Gan luo County Center for Disease Control and Prevention of Liangshan Prefecture

The Yi are the largest and most disadvantaged ethnic minority population in southwestern China. This region contains over eight million Yi, with approximately 2.4 million living in the Liangshan Yi Autonomous Prefecture （Liangshan） in Sichuan Province. Liangshan is located along one of the main drug trafficking routes from the ＇Golden Triangle＇ to northwest and central China, making it one of the largest illicit drug production and distribution centers in China.

关键词： HIV Unprotected Sex with Casual Partners A Neglected Source of HIV Transmission among Members of the Yi Minority in Southwestern China

Study on hypoglycemic and hypolipidemic effects of Dayi bacterial prescription tea

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中国药理学与毒理学杂志 2019年第10期 923-924页

作者： LI Shuang-long ZHU Yong-liang WU Meng-yuan chen dan-dan GAO Lin-rui QIU Xiang-jun Medical College of Henan University of Science and Technology Yunnan Dayi Microbial Technology Co. Ltd

OBJECTIVE To study the hypoglycemic and hypolipidemic effects of concentrated extracts of Dayi four bacterial prescription tea and also its effects on liver and kidney function by establishing a high-sugar and high-fat animal model in mice. METHODS 220 healthy male mice were randomly divided into 7 groups:normal control group（NC）, positive control group（PC）,model control group（HC） and experimental group（group A, B, C and D of Dayi bacterial prescription tea concentrated extract）; 35 mice in NC group and PC group, and30 mice in other groups, respectively. The NC group was fed with normal basic diet, while the PC group, the HC group and experimental group were fed with high-sugar and high-fat diet. The model was built for 8 weeks. Starting from the ninth week, the experimental group was given concentrated extract of Dayi Fang Tea by gavage; group A(0.318 g·kg;DYA), group B(0.3 g·kg;DYB), group C(0.222 g·kg;DYC), group D(0.298 g·kg;DYD), and the PC group was given 83.33 mg · kg;metformin and3.33 mg·kg;lovastatin by gavage. At the thirteenth and fifteenth week, the animals were killed, blood was taken,serum was separated, glucose（Glu）, total cholesterol（TC）, triglyceride（TG）, high density lipoprotein cholesterol（HDL-C）, alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, total protein（TP）, albumin（ALB）, urea and creatinine level were detected using a biochemical analyzer. RESULTS The study showed thatthe levels of serum Glu, TC and TG were significantly increased in the model group compared with the NC group（P<0.01）.Compared with the model group, the levels of serum Glu,TC and TG in the four experimental groups decreased significantly（P<0.01）, the levels of HDL-C increased,and the levels of serum insulin increased. Compared with the NC group and the PC group, the levels of serum Glu, TC and TG in the four experimental groups were increased significantly（P<0.01）. There was no significant difference in TG level. CONCLUSION The concentrated extracts of four kin

关键词： bacterium tea hyperglycemia hyperlipidemia high-sugar and high-fat diet

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共刺激信号在支气管哮喘治疗中的应用前景

中华结核和呼吸杂志 2007年第1期30卷 73-75页

作者：陈丹丹邱晨暨南大学第二临床医学院呼吸科深圳518020

支气管哮喘（简称哮喘）是由多种炎性细胞及活性因子的级联反应引起的慢性气道炎症，在这些炎性细胞中，T淋巴细胞起着核心作用。T淋巴细胞的活化是哮喘炎症级联反应的触发点，活化过程不仅需要抗原特异的识别信号，而且需要T淋巴细胞... 详细信息

支气管哮喘（简称哮喘）是由多种炎性细胞及活性因子的级联反应引起的慢性气道炎症，在这些炎性细胞中，T淋巴细胞起着核心作用。T淋巴细胞的活化是哮喘炎症级联反应的触发点，活化过程不仅需要抗原特异的识别信号，而且需要T淋巴细胞表面共刺激分子与抗原提呈细胞（APC）表面相应配体结合产生的共刺激信号。

关键词：共刺激信号支气管哮喘哮喘治疗慢性气道炎症抗原提呈细胞 T淋巴细胞表面共刺激分子级联反应

Molecular Cloning and Expression Analysis of Potassium Transporter Gene,MaHAK5,in Mulberry

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Molecular Cloning and Expression Analysis of Potassium Trans...

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

2017中国长三角遗传学大会

作者： chen dan-dan Zhao Wei-Guo Jiangsu University of Science and technology

Potassium(K +) is one of the many elements necessary for plant growth and development,and is the only one that plants need to have a higher concentration of *** order to cope with the low potassium stress,the plant evolves a series of complex signal regulation ***5 is an important high-affinity potassium transporter,which is mainly responsible for potassium uptake under low potassium conditions,and low potassium stress can significantly induce transcription of *** this experiment,the complete reading frame(ORF) of the gene was cloned from the young leaves of mulberry Yu 71-1,named *** analysis showed that the MaHAK5 is 2625 bp long,It’s open reading frame(ORF) is of 2478 bp,and encoding 828 amino acids with a predicted molecular weight of 92.17 kD and an isoelectric point of *** analysis based on MaHAK5 with other species showed that mulberry had a closer relationship with Chuansang(Moras notabilis XP10112002.1),Jujube(Ziziphus jujuba XP15898221.1),Fragaria vesca(Fragaria vesca *** XP04299195.2).Drought,high salt and low temperature stress could affect the expression level of MaHAK5 gene in mulberry,and the expression level of MaHAK5 gene was increased and the physiological and biochemical indicators were affected by low potassium treatment.

关键词： Mulberry MaHAK5 Gene cloning Sequence characteristics Gene expression Physiological and biochemical indicators

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Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying chen Yinyi Xiong Qin Zhou Ling-Qiang Zhu dan Liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

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《尚书》轴心话语的现代阐释及跨文化传译

厦门大学学报（哲学社会科学版） 2018年第3期68卷 88-99页

作者：陈丹丹扬州大学外国语学院江苏扬州225009

《尚书》作为中国乃至世界最早的"政史之典",是历代阐释的重要对象,也是西方汉学家最早关注并翻译的中国经典之一。"德"与"天"是《尚书》的轴心话语,反映中华文明核心思想,是《尚书》诠释的关节点。从现代诠释学的视角进行研究发现:"德... 详细信息

《尚书》作为中国乃至世界最早的"政史之典",是历代阐释的重要对象,也是西方汉学家最早关注并翻译的中国经典之一。"德"与"天"是《尚书》的轴心话语,反映中华文明核心思想,是《尚书》诠释的关节点。从现代诠释学的视角进行研究发现:"德"字从甲骨初文到现代定型过程中的不断阐释带来了意义的突破,实现了其含义从描摹人类活动到具有哲学特征的意识形态意义的建立;"天"因其文字来源始终与一种至上的神秘力量紧密联系,最终发展上升成为一种哲学命题,铸造出中国人的思维模式。"德"和"天"的传译也是一种阐释,译者和文本在历史性相遇和融合的过程中进行着意义的消解和建构。成功的译文应该基于合理的文本阐释,将中华思想概念表达出来,传译出去,这样便达到了典籍外译的目的。

关键词：尚书轴心话语诠释学现代阐释跨文化传译

机械化学法制备金属-有机骨架及其复合物研究进展

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无机化学学报 2020年第10期36卷 1805-1821页

作者：陈丹丹衣晓虹王崇臣北京建筑大学建筑结构与环境修复功能材料北京市重点实验室北京100044 北京建筑大学环境与能源工程学院北京100044

金属-有机骨架(MOFs)是由金属离子/簇和多齿状有机配体通过配位键桥联而形成的多孔晶态材料。MOFs材料具有孔隙率高、比表面积大、尺寸可调、结构易修饰、功能多样化等特点,使其在气体吸附、分离和催化等方面都具有潜在应用价值。到目... 详细信息

金属-有机骨架(MOFs)是由金属离子/簇和多齿状有机配体通过配位键桥联而形成的多孔晶态材料。MOFs材料具有孔隙率高、比表面积大、尺寸可调、结构易修饰、功能多样化等特点,使其在气体吸附、分离和催化等方面都具有潜在应用价值。到目前为止,在MOFs合成的几种常见方法中,机械化学法(即在无溶剂或极少量溶剂的情况下研磨固体反应物进行的反应)作为一种清洁、绿色、高效的合成手段逐渐引起人们的关注。本综述总结了近年来机械化学合成MOFs及其复合物的典型进展,目的是为机械化学法合成MOFs及其复合材料提供一个通用而易于理解的概述。目前的研究进展表明,机械化学法是一种实用且环境友好的合成方法,为低成本、宏量生产MOFs及其复合物提供了可能。

关键词：金属-有机骨架复合物机械化学法宏量合成