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Mutation in a non-force-bearing region of protein L influences force-dependent unfolding behavior

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Chinese Physics B 2024年第7期33卷 624-629页

作者：蒋环杰王艳伟陈家媛胡丹潘海郭子龙陈虎 Department of Physics Wenzhou UniversityWenzhou 325035China Center of Biomedical Physics Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325000China Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Laboratory for Soft Functional Materials ResearchDepartment of PhysicsXiamen UniversityXiamen 361005China

Single-molecule magnetic tweezers(MTs) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MTs to measure the force-dependent folding and unfolding rates of both protein L(PLWT) and its Y47W mutant(PLY47W) where the mutation point is not at the force-bearing β-strands. The measurements were conducted within a force range of 3–120 pN. Notably, the unfolding rates of both PLWT and PWY47W exhibit distinct force sensitivities below 50 pN and above 60 pN, implying a two-barrier free energy landscape. Both PLWT and PLY47W share the same force-dependent folding rate and the same transition barriers,but the unfolding rate of PLY47W is faster than that of PLWT. Our finding demonstrates that the residue outside of the force-bearing region will also affect the force-induced unfolding dynamics.

关键词： protein folding magnetic tweezers protein L

The new insights of hyperbaric oxygen therapy:focus on inflammatory bowel disease

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Precision Clinical Medicine 2024年第2期7卷 159-169页

作者： Leilei chen Yan Wang Huihui Zhou Yi Liang Fengqin Zhu Guangxi Zhou Cheeloo College of Medicine Shandong UniversityJinan 250012China Department of Gastroenterology Affliated Hospital of jining Medical University Jining Medical UniversityJining 272000China Department of Hyperbaric Oxygen Affliated Hospital of jining Medical UniversityJining Medical UniversityJining 272000China

Inflammatory bowel diseases(IBD),with an increasing incidence,pose a significant health *** there have been significant advances in the treatment of IBD,more progress is still *** oxygen therapy(HBOT)has been shown to treat a host of conditions such as carbon monoxide poisoning,decompression sickness,and gas *** the last few years,there has been an increase in research into the use of HBOT as an adjunct to conventional treatment for *** research has shown that HBOT may exert its therapeutic effects by decreasing oxidative stress,inhibiting mucosal inflammation,promoting ulcer healing,influencing gut microbes,and reducing the incidence of IBD *** paper aims to provide a comprehensive review of experimental and clinical trials exploring HBOT as a supplement to IBD treatment strategies.

关键词： hyperbaric oxygen therapy inflammatory bowel disease mucosal inflammation

Multi-Label Feature Selection Based on Improved Ant Colony Optimization Algorithm with Dynamic Redundancy and Label Dependence

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Computers, Materials and Continua 2024年第1期81卷 1157-1175页

作者： Cai, Ting Ye, Chun Ye, Zhiwei chen, Ziyuan Mei, Mengqing Zhang, Haichao Bai, Wanfang Zhang, Peng School of Computer Hubei University of Technology Wuhan 430068 China Xining Big Data Service Administration Xining 810000 China Wuhan Fiberhome Technical Services Co. Ltd Wuhan 430205 China

The world produces vast quantities of high-dimensional multi-semantic data. However, extracting valuable information fromsuch a largeamount of high-dimensional andmulti-label data is undoubtedly arduous and challenging. Feature selection aims to mitigate the adverse impacts of high dimensionality in multi-label data by eliminating redundant and irrelevant features. The ant colony optimization algorithmhas demonstrated encouraging outcomes in multi-label feature selection, because of its simplicity, efficiency, and similarity to reinforcement learning. Nevertheless, existing methods do not consider crucial correlation information, such as dynamic redundancy and label correlation. To tackle these concerns, the paper proposes a multi-label feature selection technique based on ant colony optimization algorithm (MFACO), focusing on dynamic redundancy and label correlation. Initially, the dynamic redundancy is assessed between the selected feature subset and potential ***, the ant colony optimization algorithmextracts label correlation fromthe label set,which is then combined into the heuristic factor as label weights. Experimental results demonstrate that our proposed strategies can effectively enhance the optimal search ability of ant colony, outperforming the other algorithms involved in the paper. © 2024 The Authors. Published by Tech Science Press.

关键词： Ant colony optimization

Single-cell transcriptomics reveals cell atlas and identifies cycling tumor cells responsible for recurrence in ameloblastoma

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International Journal of Oral Science 2024年第2期16卷 251-264页

作者： Gan Xiong Nan Xie Min Nie Rongsong Ling Bokai Yun Jiaxiang Xie Linlin Ren Yaqi Huang Wenjin Wang chen Yi Ming Zhang Xiuyun Xu Caihua Zhang Bin Zou Leitao Zhang Xiqiang Liu Hongzhang Huang Demeng chen Wei Cao cheng Wang Hospital of Stomatology Sun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Stomatology Sun Yat-sen UniversityGuangzhouChina Guanghua School of Stomatology Sun Yat-sen UniversityGuangzhouChina Department of Periodontics Affiliated Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineGuangzhouChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Center for Translational Medicine The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhouChina Department of Oral and Maxillofacial Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouChina Department of Oral and Maxillofacial&Head and Neck Oncology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina National Center for Stomatology National Clinical Research Center for Oral diseasesShanghai Key Laboratory of StomatologyShanghaiChina

Ameloblastoma is a benign tumor characterized by locally invasive phenotypes,leading to facial bone destruction and a high recurrence ***,the mechanisms governing tumor initiation and recurrence are poorly ***,we uncovered cellular landscapes and mechanisms that underlie tumor recurrence in ameloblastoma at single-cell *** results revealed that ameloblastoma exhibits five tumor subpopulations varying with respect to immune response(IR),bone remodeling(BR),tooth development(TD),epithelial development(ED),and cell cycle(CC)*** note,we found that CC ameloblastoma cells were endowed with stemness and contributed to tumor recurrence,which was dominated by the EZH2-mediated *** EZH2 effectively eliminated CC ameloblastoma cells and inhibited tumor growth in ameloblastoma patient-derived *** data described the tumor subpopulation and clarified the identity,function,and regulatory mechanism of CC ameloblastoma cells,providing a potential therapeutic target for ameloblastoma.

关键词： inhibited cycling eliminated

Biallelic variants in RBM42 cause a multisystem disorder with neurological,facial,cardiac,and musculoskeletal involvement

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Protein & Cell 2024年第1期15卷 52-68页

作者： Yiyao chen Bingxin Yang Xiaoyu Merlin Zhang Songchang chen Minhui Wang Liya Hu Nina Pan Shuyuan Li Weihui Shi Zhenhua Yang Li Wang Yajing Tan Jian Wang Yanlin Wang Qinghe Xing Zhonghua Ma Jinsong Li He-Feng Huang Jinglan Zhang chenming Xu Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China bstetrics and Gynecology Hospital Institute of Reproduction and DevelopmentFudan UniversityShanghai 200011China Intermational Peace Matermity and Child Health Hospital School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Shanghai Key Laboratory of Embryo Original Diseases Shanghai 200030China State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCAS Center for Excellence in Molecular Cell ScienceUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China State Key Laboratory of Rice Biology the Key Laboratory of Molecular Biology of Crop Pathogens and InsectsInstitute of BiotechnologyZhejiang UniversityHangzhou 310058China Verna and Marrs McLean Department of Biochemistry and Molecular Biology Baylor College of MedicineHoustonTX 77030USA Children's hospital of Fudan University Shanghai 201102China School of Life Science and Technology Shanghai Tech UniversityShanghai 201210China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024ZhejiangChina Research Units of Embryo Original Diseases Chinese Academy of Medical Sciences(No.2019RU056)Shanghai 200011China

Here,we report a previously unrecognized syndromic neurodevelopmental disorder associated with biallelic loss-of-function variants in the RBM42 *** patient is a 2-year-old female with severe central nervous system(CNs)abnormalities,hypotonia,hearing loss,congenital heart defects,and dysmorphic facial *** whole-exome sequencing(WEs)reveals that the patient has two compound heterozygous variants,c.304C>T(p.R102*)and c.1312G>A(p.A438T),in the RBM42 gene which encodes an integral component of splicing complex in the RNA-binding motif protein *** p.A438T variant is in the RRM domain which impairs RBM42 pro-tein stability in ***,p.A438T disrupts the interaction of RBM42 with hnRNP K,which is the causa-tive gene for Au-Kline syndrome with overlapping disease characteristics seen in the index *** human R102*or A438T mutant protein failed to fully rescue the growth defects of RBM42 ortholog knockout△FgRbp1 in Fusarium while it was rescued by the wild-type(WT)human RBM42.A mouse model carying Rbm42 compound heterozygous variants,c.280C>T(p.Q94*)and c.1306_1308delinsACA(p.A436T),demonstrated gross fetal develop-mental defects and most of the double mutant animals died by ***-seq data confirmed that Rbm42 was involved in neurological and myocardial functions with an essential role in alternative splicing(As).Overall,we present clinical,genetic,and functional data to demonstrate that defects in RBM42 constitute the underlying etiology of a new neurodevelopmental disease which links the dysregulation of global AS to abnormal embryonic development.

关键词： RBM42 gene RNA-binding protein neurodevelopmental disorder Au-Kline syndrome alternative splicing

Cost-efficient quantum access network boosts practical deployment of quantum key distribution network

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 169-169页

作者： Hua-Lei Yin Zeng-Bing chen Department of Physics and Beijing Key Laboratory of Opto-electronic Functional Materials and Micro-nano Devices Key Laboratory of Quantum State Construction and Manipulation(Ministry of Education)Renmin University of ChinaBeijing 100872China National Laboratory of Solid State Microstructures and School of Physics Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China

The security of information is not assured over extended periods,especially with the rapid advancement of quantum *** address this challenge,quantum cryptography,rooted in quantum physics,offers comprehensive toolkits for key distribution[1],secret sharing[2],digital signatures[3]and other cryptographic tasks[4]with information-theoretical *** feasibility of quantum cryptography,particularly quantum key distribution(QKD)。

关键词： quantum distribution signature

In situ single iron atom doping on Bi_(2)WO_(6)monolayers triggers efficient photo-fenton reaction

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Environmental Science and Ecotechnology 2024年第4期20卷 315-322页

作者： Wei Liu Peifang Wang Juan chen Xin Gao Huinan Che Xiaozhi Su Bin Liu Yanhui Ao Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes Ministry of EducationCollege of EnvironmentHohai UniversityNo.1Xikang RoadNanjing210098China School of Chemical and Biomedical Engineering Nanyang Technological UniversitySingapore637459Singapore Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai201204China

Developing an efficient photocatalytic system for hydrogen peroxide(H_(2)O_(2))activation in Fenton-like processes holds significant promise for advancing water purification ***,challenges such as high carrier recombination rates,limited active sites,and suboptimal H_(2)O_(2)activation efficiency impede optimal *** we show that single-iron-atom dispersed Bi_(2)WO_(6)monolayers(SIAD-BWOM),designed through a facile hydrothermal approach,can offer abundant active sites for H_(2)O_(2)*** SIAD-BWOM catalyst demonstrates superior photo-Fenton degradation capabilities,particularly for the persistent pesticide dinotefuran(DNF),showcasing its potential in addressing recalcitrant organic *** reveal that the incorporation of iron atoms in place of tungsten within the electron-rich[WO_(4)]^(2-)layers significantly facilitates electron transfer processes and boosts the Fe(II)/Fe(III)cycle *** experimental investigations and theoretical analyses further elucidate how the atomically dispersed iron induces lattice strain in the Bi_(2)WO_(6)monolayer,thereby modulating the d-band center of iron to improve H_(2)O_(2)adsorption and *** research provides a practical framework for developing advanced photo-Fenton catalysts,which can be used to treat emerging and refractory organic pollutants more effectively.

关键词： H_(2)O_(2)activation Water treatment Bismuth tungstate Photo-fenton catalysts Emerging organic pollutants

Deep learning-based modeling method for probabilistic LCF life prediction of turbine blisk

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Propulsion and Power Research 2024年第1期13卷 12-25页

作者： cheng-Wei Fei Yao-Jia Han Jiong-Ran Wen chen Li Lei Han Yat-Sze Choy Department of Aeronautics and Astronautics Fudan UniversityShanghai 200433China Department of Mechanical Engineering Hong Kong Polytechnic UniversityHong Kong 999077China

Turbine blisk is one of the typical components of gas turbine *** fatigue life of turbine blisk directly affects the reliability and safety of both turbine blisk and aeroengine *** monitor the performance degradation of an aeroengine,an efﬁcient deep learning-based modeling method called convolutional-deep neural network(C-DNN)method is proposed by absorbing the advantages of both convolutional neural network(CNN)and deep neural network(DNN),to perform the probabilistic low cycle fatigue(LCF)life prediction of turbine blisk regarding uncertain influencing *** the C-DNN method,the CNN method is used to extract the useful features of LCF life data by adopting two convolutional layers,to ensure the precision of C-DNN *** two close-connected layers in DNN are employed for the regression modeling of aeroengine turbine blisk LCF life,to keep the ac-curacy of LCF life *** the probabilistic analysis of turbine blisk and the com-parison of methods(ANN,CNN,DNN and C-DNN),it is revealed that the proposed C-DNN method is an effective mean for turbine blisk LCF life prediction and major factors affecting the LCF life were gained,and the method holds high efﬁciency and accuracy in regression modeling and *** study provides a promising LCF life prediction method for complex structures,which contribute to monitor health status for aeroengines operation.

关键词： Convolutional-deep neural network Low cycle fatigue Life prediction Turbine blisk Probabilistic prediction

Genomic profiling of colorectal cancer in large-scale Chinese patients:amplification and somatic mutations in ERBB2

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Oncology Research 2024年第9期32卷 1429-1438页

作者： YUZHI LIU EVELYNE BISCHOF ZHIQIN chen JIAHUAN ZHOU BEI ZHANG DING ZHANG YONG GAO MING QUAN Department of Oncology Shanghai East HospitalTongji University School of MedicineShanghaiChina State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer InstituteDepartment of OncologyRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Jiangsu Hengrui Pharmaceuticals Co. Ltd.ShanghaiChina D Medicines Inc. ShanghaiChina

Objectives:Human epidermal growth factor receptor 2(HER2)-targeted therapies have demonstrated potential benefits for metastatic colorectal cancer(mCRC)patients with HER2 amplification,but are not satisfactory in cases of HER2 mutant ***:Consequently,further elucidation of amplifications and somatic mutations in erythroblastic oncogene B-2(ERBB2)is *** genomic profiling was conducted on 2454 Chinese CRC cases to evaluate genomic alterations in 733 cancer-related genes,tumor mutational burden,microsatellite instability,and programmed death ligand 1(PD-L1)***:Among 2454 CRC patients,85 cases(3.46%)exhibited ERBB2 amplification,and 55 cases(2.24%)carried ERBB2 mutation.p.R678Q(28%),p.V8421(24%),and p.S310F/Y(12%)were the most prevalent of the 16 detected mutation *** comparison to the ERBB2 altered(alt)group,KRAS/BRAF mutations were more prevalent in ERBB2 wild-type(wt)samples(ERBB2wt ***2alt,KRAS:50.9%vs.25.6%,p<0.05;BRAF:8.5%vs.2.3%,p<0.05).32.7%(18/55)of CRCs with ERBB2 mutation exhibited microsatellite instability high(MSI-H),while no cases with HER2 amplification displayed *** genes varied between ERBB2 copy number variation(CNV)and ERBB2 single nucleotide variant(SNV);TP53 alterations tended to co-occur with ERBB2 amplification(92.3%)as opposed to ERBB2 mutation(58.3%).KRAS and PIK3CA alterations were more prevalent in ERBB2 SNV cases(KRAS/PIK3CA:45.8%/31.2%)compared to ERBB2 amplification cases(KRAS/PIK3CA:14.1%/7.7%).Conclusion:Our study delineates the landscape of HER2 alterations in a large-scale cohort of CRC patients from *** findings enhance our understanding of the molecular features of Chinese CRC patients and offer valuable implications for further investigation.

关键词： Colorectal cancer ERBB2 HER2 Human epidermal growth factor receptor 2 Genetic profiling Precision oncology