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Combined effect of tributyltin and benzo[a] pyrene on the levels of sex hormone and vitellogenin in female Sebastiscus marmoratu

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Journal of Environmental Sciences 2006年第2期18卷 359-363页

作者： ZHENG Rong-hui WANG Chong-gang ZUO Zheng-hong chen yi-xin ZHAO Yang School of Life-Sciences Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

Tributyltin（TBT）, an organometal used as an antifouling biocide, has been reported to induce masculinization of fish. Benzo [a]pyrene （BaP）, a widespread carcinogenic polycyclic aromatic hydrocarbon, has been reported that its microsomal metabolites can produce an estrogenic response when tested in vitro. This study was therefore designed to examine the potential in vivo influence of TBT, BaP and their mixture on sex hormone levels in serum of Sebastiscus marmoratus, which were given 2 separate intraperitoneally （ip） injections（a single injection every 7 d） of TBT（0.5, 1, 5 and 10 mg/kg）, BaP（0.5, 1, 5 and 10 mg/kg）, or both in combination（0.5, 1, 5 and 10 mg/kg）; control fish received olive oil vehicle only. Six days after the 2nd injection, serum samples were collected and analyzed for sex hormone levels and alkali labile protein phosphorus （ALPP）, which is related to the yolk precursor protein vitellogenin. The pollutants at all doses significantly reduced serum testosterone, estradiol and ALPP content after 2 injections compared with the corresponding controls. The reduction of the estradiol levels should be response for the decrease of the vitellogenin levels. The results in the present study suggested that aromatase seems not the major target acted by TBT and BaP in fish. This study demonstrated that TBT or BaP exposure both inhibit the reproductive potential in female Sebastiscus marmoratus. Combined effect of TBT and BaP on the serum testosterone, estradiol and ALPP was not antagonism from the anticipation.

关键词： tributyltin benzo[a]pyrene estosterone 17β-estradiol vitellogenin Sebastiscus marmoratus

Antioxidant responses to benzo[a]pyrene,tributyltin and their mixture in the spleen of Sebasticus marmoratus

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Journal of Environmental Sciences 2007年第9期19卷 1129-1135页

作者： WU Yu-qiong WANG Chong-gang WANG Yun ZHAO Yang chen yi-xin ZUO Zheng-hong Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 China. Bioengineering Department MinBei Vocational and Technical College Jianyang 354200 China

It has been reported that there is an interaction between Benzo[a]pyrene （BaP）, a widespread carcinogenic polycyclic aromatic hydrocarbon, and tributyltin （TBT）, an organometal used as an antifouling biocide. This study was therefore designed to examine the potential in vivo influence of BaP, TBT and their mixture on splenic antioxidant defense systems of Sebastiscus marmoratus. The fish were exposed to water containing environmentally relevant concentrations of BaP, TBT and their mixture. Spleens were collected for biochemical analysis after exposure for 7, 25, 50 d and after recovery for 7, 20 d. Cotreatment with BaP and TBT for 7 d potentiated the induction of glutathione peroxidase （GPx） activity by BaP or TBT alone. The cotreatment for 25 and 50 d resulted in inhibition of GPx activity, which was similar to the effect of TBT. Splenic glutathione S-transferase （GST） activities were significantly elevated in S. marmoratus exposed to BaP starting from 7 d and remained high up to 25 d. However, no further activity change was found with prolonged exposure. Cotreatment of BaP and TBT primarily inhibited the GST activity, which was similar to the effect of TBT. Cotreatment with BaP and TBT for 25 or 50 d potentiated the depletion of GSH （glutathione） by BaP or TBT alone. MDA （malondialdehyde） contents in spleen of S. marmoratus were not significantly altered compared with the control during the test period. Spleen, as an immune organ, is sensitive to exposure of BaP or TBT. It should have an effective mechanism to counteract oxidative damage. Antioxidative defense systems in spleen of S. marmoratus should be considered as potential biomarkers. Short-term exposure of BaP or TBT could result in induction of antioxidant defense system. A significant decrease of these indices, such as GSH, GST, GPx might indicate more severe contamination.

关键词： tributyltin benzo[a]pyrene antioxidant defense combined effect Sebastiscus marmoratus

Hydrogen sulfide:third gaseous transmitter,but with great pharmacological potential

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Acta Pharmacologica Sinica 2007年第11期28卷 1709-1716页

作者： Chang-qing chen Hong xin yi-zhun ZHU Department Of Pharmacology,School of Pharmacy,Fudan University Shanghai 200032,China Department of Pharmacology School of Pharmacy Fudan University Shanghai China

Hydrogen sulfide (H2S),which is well known traditionally as a toxic gas,has beenproven to be produced endogenously by 3 enzymes in mammalian tissues andplays important roles in physiological and pathophysiological *** thecentral nervous system,H2S functions as not only a neuromodulator,but also aneuroprotectant against oxidative *** the cardiovascular system,H2S re-laxes vascular smooth muscles by the activation of KATPchannels and inhibitssmooth muscle cell proliferation via the mitogen-activated protein kinase signal-ing *** effects are important for maintaining blood pressure andpreventing vessel structural remodeling,and identifies H2S as an important factorin the development of some vascular diseases,such as hypertension.H2S alsoshows cardioprotective effects in ischemic myocardium and septic and *** studies have demonstrated a new mechanism to explain the motoreffect of H2S on the rat detrusor muscle,which is through the activation of thecapsaicin-sensitive primary *** review focuses on the recent researchachievements on H2S and discloses the great potential of H2S as the third gaseoustransmitter in cardiac protection.

关键词： hydrogen sulfide cystathionine β-synthetase cystathionine γ-lyase KATP channels capsaicin

Modified Sijunzi Granules Exhibit Hemostatic Effect by Activating Akt and Erk Signal Pathways via Regulating 5-HT and Its Receptors Levels

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Chinese Journal of Integrative Medicine 2024年第12期30卷 1121-1127页

作者： WANG Jun ZHANG Xue-ying KANG Yan-hong ZHANG Yun chen xin-yi ZHOU Jia-li MA Wei Department of Hematology the First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou 310006China Department of Hemato-Oncology Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100700China Hunter Biotechnology Inc.Hangzhou 310051China

Objective:To investigate the hemostatic effect of modified Sijunzi Granules(MSG)in primary immune thrombocytopenia(ITP)zebrafish model and explore the potential ***:AB strain wild type zebrafish were treated with simvastatin(6μmol/L)for 24 h to establish the hemorrhage model(model control group).The zebrafish were treated with MSG at different doses(55.6,167,and 500μg/mL),*** hemostatic effect was assessed by examining the intestinal bleeding and hemostatic rate.5-hydroxytryptamine(5-HT)content was determined using enzyme-linked immunosorbent assay(ELISA)*** expressions of5-HT2aR,5-HT2bR,and SERT genes were detected by quantitative real-time polymerase chain reaction(PCR).The protein expressions of protein kinase B(Akt),p-Akt,extracellular regulated protein kinases(Erk),and p-Erk were examined using Western blot ***:The intestinal bleeding rate was 37%,40%,and 80%in the55.6,167,and 500μg/mL dose of MSG,respectively,in which 55.6 and 167μg/mL MSG dose groups were associated with significantly decreased intestinal bleeding rate when compared with the model control group(70%,P<0.05).Significantly higher hemostatic rates were also observed in the 55.6μg/mL(54%)and 167μg/mL(52%)MSG dose groups(P<0.05).MSG increased the 5-HT content and mRNA expression levels of 5-HT2aR,5-HT2bR,and SERT(P<0.05).In addition,caspase3/7 activity was inhibited(P<0.05).Significant increase in p-Akt and p-Erk was also detected after treatment with MSG(P<0.05).Conclusions:MSG could reduce the incidence and severity of intestinal bleeding in zebrafish by activating MAPK/Erk and PI3K/Akt signal pathways through regulating the levels of 5-HT and its receptors,which may provide evidence for the treatment of ITP.

关键词： modified Sijunzi Granules Chinese medicine primary immune thrombocytopenia zebrafish 5-hydroxytryptamine 5-hydroxytryptamine receptor mitogen-activated protein kinase/extracellular regulated protein kinases signal pathway phosphoinositide3-kinases/protein kinase B signal pathway

LONG-TERM OBSERVATION FOR OSSEOINTEGRATION OF CALCIUM PHOSPHATE SOL-GEL COATING ON Ti-6Al-4V IMPLANTS

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Chinese Medical Sciences Journal 2007年第3期22卷 183-186页

作者： cheng Yang Yuan-ying Li Li-e Meng Jing-xin chen yi Huang Stomatology Center Union Hospital Tongji Medical College Huazhong University of Science ＆ Technology Wuhan 430022

Objective To observe the long-term impact of calcium phosphate （CaP） sol-gel coating on bone growth around porous-surfaced implant. Methods The porous-surfaced Ti-6Al-4V implants were prepared with the addition of a thin film of CaP sol-gel coating, and implanted into the tibiae of 8 rabbits, each with two implants. Implanted sites were allowed to heal for 2, 8, 12, and 24 weeks, after which specimens were obtained for scanning electron microscope analysis using the freezefracture technique. Results The sol-gel coated implants recovered by freeze-fracture technique showed extensive bone growth from the endosteum along the implant surface. The bone was in direct contact with the CaP layer. The cement line-like layer was clearly demonslxated to be an intervening electron dense afibrillar layer between the CaP coat and the overlying newly deposited bone. The stability and osseointegration of the porous-surfaced implants seemed not to be affected by the osteoclastic resorption of CaP layer occurred during 24 weeks of healing. Conclusion Based on the findings in the long-term observation, the addition of a thin layer of CaP promotes an extensive osseointegrated interface between the porous-surfaced Ti-6Al-4V implants and the newly deposited bone.

关键词： calcium phosphate sol-gel implant osteoconduction scanning electron microscopy

Effects of Compound Zhebei Granule(复方浙贝颗粒) Combined with Doxorubicin on Expression of Specific Surface Antigens in Mice with Transplanted KG-1a Cells

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Chinese Journal of Integrative Medicine 2018年第3期24卷 213-217页

作者： ZHANG Yu HOU li chen xin-yi Department of Integrated Chinese and Western Medicine Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for Cancer Tianjing 300060China Key Laboratory of Cancer Prevention and Therapy Tianjin 300060 China Tianjin Clinical Research Center for Cancer Tianjin 300060 China Department of Hematology Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine Beijinq 100700 China

Objective：To investigate the effects of Compound Zhebei Granule（复方浙贝颗粒,CZG）combined with doxorubicin hydrochloride（adriamycin,ADM）on specific surface antigens in mice with KG-1a transplanted ***：A subcutaneous tumor xenograft model was established by injection of the acute myeloid leukemia cell line KG-1a into the axillary flanks of BALB/c-nude ***-four tumor bearing mice were divided into 4groups according to a random number table,including normal saline control group,ADM group,high-dose CZG group,and mid-dose CZG group,with 6 mice in each *** administration occurred on the 14th day after cell inoculation,and normal saline control group mice were gavaged with normal saline at 0.2 m L/10 g every other *** group mice were intraperitoneally injected with ADM 1 mg/kg[conversion of adults,40 mg/（m^2·d）]every other ***-and mid-dose CZG groups mice were gavaged with CZG at the dose of 8 and 4 g/kg respectively every other day and intraperitoneally injected with ADM（1 mg/kg）every other *** administration period lasted for10 *** tumor xenografts were made into mononuclear cell suspensions after dissection,and the expressions of specific surface antigens,including CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋,in KG-1a cell line tumor xenografts were detected by flow ***：Compared with the control and ADM groups,expression of CD34~＋CD38^-in the two CZG groups was significantly lower（P〈0.05）.Compared with the control group,expression of CD34~＋CD38^-CD123~＋in the two CZG groups decreased（P〈0.05）.The high-dose CZG group showed more obvious outcomes compared with the ADM group（P〈0.05）.Compared with the control and ADM groups,the expression of CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in the two CZG groups decreased（P〈0.05）.Conclusions：CZG combined with doxorubicin could reduce the expression of CD34~＋CD38^-,CD34~＋CD38^-CD123~＋,CD34~＋CD38^-CD96~＋and CD34~＋CD38^-CD33~＋in KG-1a cell line tumor xenograf

关键词： acute myeloid leukemia Chinese medicine doxorubicin adriamycin

Efficiency-enhanced AlGaInP Light-Emitting Diodes with Thin Window Layers and Coupled Distributed Bragg Reflectors

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Chinese Physics Letters 2011年第6期28卷 305-307页

作者： chen yi-xin SHEN Guang-Di ZHU Yan-Xu GUO Wei-Ling LI Jian-Jun Optoelectronic Technology Laboratory Beijing University of TechnologyBeijing 100124

A new structure of high-brightness light-emitting diodes(LED)is experimentally *** thin window layer is composed of a 300-nm-thick indium-tin-oxide layer and a 500-nm-thick GaP layer for both current spreading and light *** two coupled distributed Bragg reflectors(DBRs)with one for reflecting normal incidence light and the other for reflecting inclined incidence light which is emitted to the GaAs substrate are employed in the LED *** coupled DBRs in the LED can provide high reflectivity with wide-angle *** efficiency-enhanced AlGaInP LED has the luminance intensity increase of more than 50%compared with conventional LEDs and high reliability with the saturation current 130 mA.

关键词： Efficiency thick reflectivity

High-throughput screening of CO_(2) cycloaddition MOF catalyst with an explainable machine learning model

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Green Energy & Environment 2025年第1期10卷 132-138页

作者： Xuefeng Bai yi Li Yabo Xie Qiancheng chen xin Zhang Jian-Rong Li Beijing Key Laboratory for Green Catalysis and Separation and Department of Chemical Engineering College of Materials Science&EngineeringBeijing University of TechnologyBeijing100124China

The high porosity and tunable chemical functionality of metal-organic frameworks(MOFs)make it a promising catalyst design ***-throughput screening of catalytic performance is feasible since the large MOF structure database is *** this study,we report a machine learning model for high-throughput screening of MOF catalysts for the CO_(2) cycloaddition *** descriptors for model training were judiciously chosen according to the reaction mechanism,which leads to high accuracy up to 97%for the 75%quantile of the training set as the classification *** feature contribution was further evaluated with SHAP and PDP analysis to provide a certain physical understanding.12,415 hypothetical MOF structures and 100 reported MOFs were evaluated under 100℃ and 1 bar within one day using the model,and 239 potentially efficient catalysts were *** them,MOF-76(Y)achieved the top performance experimentally among reported MOFs,in good agreement with the prediction.

关键词： Metal-organic frameworks High-throughput screening Machine learning Explainable model CO_(2)cycloaddition

Targeted bile acids and gut microbiome profiles reveal the hepatoprotective effect of WZ tablet(Schisandra sphenanthera extract) against LCA-induced cholestasis

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Chinese Journal of Natural Medicines 2020年第3期18卷 211-218页

作者： LI Dong-Shun HUANG Quan-Fei GUAN Li-Huan ZHANG Hui-Zhen LI Xi FU Kai-Li chen yi-xin WAN Jian-Bo HUANG Min BI Hui-Chang Guangdong Provincial Key Laboratory of New Drug Design and Evaluation School of Pharmaceutical SciencesSun Yat-Sen UniversityGuangzhou 510000China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao 999078China

Cholestasis is caused by the obstacle of bile formation or secretion and can develop into severe liver diseases. We previously reported the ethanol extract of Schisandra sphenanthera(Wuzhi tablet, WZ) can significantly protect against lithocholic acid(LCA)-induced intrahepatic cholestasis in mice, partially due to the activation of PXR pathway and promotion of liver ***, the effect of WZ on the bile acids profile and gut microbiome in cholestastic mice remain unknown. In this study, the effect of WZ against LCA-induced liver injury was evaluated and its effect on the bile acids metabolome and gut microbiome profiles in cholestastic mice was further investigated. Targeted metabolomics analysis was performed to examine the change of bile acids in the serum, liver, intestine and feces. The change of intestinal flora were detected by the genomics method. Targeted metabolomics analysis revealed that WZ enhanced the excretion of bile acids from serum and liver to intestine and feces. Genomics analysis of gut microbiome showed that WZ can reverse LCA-induced gut microbiome disorder to the normal level. In conclusion, WZ protects against LCAinduced cholestastic liver injury by reversing abnormal bile acids profiles and alteration of gut microbiome.

关键词： Cholestasis Wuzhi tablet Schisandra sphenanthera Metabolome Gut microbiome Bile acids