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Screening and evaluation for antibiosis resistance of the spring wheat accessions to the grain aphid,Sitobion miscanthi(Takahashi)(Hemiptera:Aphididae)

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Journal of Integrative Agriculture 2022年第8期21卷 2329-2344页

作者： Kifle Gebreegziabiher GEBRETSADIK ZHANG Yong chen ju-lian State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Tigray Agricultural Research Institute Mekelle 492Ethiopia

Resistant cultivar deployment is an effective method for cereal aphid *** greenhouse conditions,preliminary antibiosis resistance screening was conducted on 114 Ethiopian and 22 Chinese spring wheat *** performing a bioassay to determine antibiosis resistance,aphid feeding behaviour and phenolic acid content analyses were performed on the aphid resistant wheat accessions by electrical penetration graph(EPG)and high performance liquid chromatography(HPLC),*** the wheat accessions,two high resistances,27moderate-resistances,and 35 low-resistances to Sitobion miscanthi were *** antibiosis resistance test showed prolonged pre-adult and pre-reproductive periods,shorter reproductive periods,lower fecundity,an intrinsic rate(rm)of increase,and a finite rate(λ)of increase of *** on Lunxuan 145,Wane,Lunxuan 6,204511,Lunxuan 103and 5215 than those on the aphid-susceptible accession Beijing *** changes for the parameters of aphid feeding behaviour,including spending a longer time in the penetration and phloem salivation phases and less time in the phloem sap-feeding phase on the resistant wheat accessions,indicated that the aphid resistance may occur during the phloem phase and may be due to difficulties in the mechanical probing of the mesophyll ***,the HPLC analysis showed higher contents of:1)ferulic acid in Lunxuan 145,Lunxuan 103 and Lunxuan 6;2)p-coumaric acid in Lunxuan145;3)vanillic acid in Lunxuan 145,Wane and Lunxuan 6;4)syringic acid in Lunxuan 103;and 5)caffeic acid in *** contents of some phenolic acids within wheat leaves,such as p-courmaric acid and vanillic acid showed significant positive correlation with the duration of aphid development,but negative correlation with the aphid *** concentrations of these acids may be the causes of antibiosis resistance to *** identification of grain aphid-resistant wheat accessions in our study will be helpful in future b

关键词： Sitobion miscanthi spring wheat accessions antibiosis resistance electronic penetration graph high-performance liquid chromatography phenolic acid concentrations

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Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum

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Journal of Integrative Agriculture 2015年第4期14卷 698-705页

作者： ZHANG Yong FAN Jia SUN Jing-rui chen ju-lian State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

The full-length c DNA of functionally-unknown salivary protein C002 in Schizaphis graminum was cloned using rapid amplification of c DNA ends（RACE） and designated as Sg C002（Gen Bank accession no. KC977563）. It is 767 bp long and encodes a protein of 190 amino acid residues with a predicted mass of 21.5 k Da and a predicted cleavage site of N-terminal signal peptide between the 24 th and the 25 th residues. Sg C002 is specifically expressed in salivary gland with the highest level at the 2nd instar. Introducing Sg C002-specific 476-si RNA, but not 546-si RNA to aphids through artificial diet significantly suppressed Sg C002 expression. Silencing Sg C002 gene led to lethality of the aphid on wheat plants, but not on pure artificial diet. Our study demonstrated that artificial diet-mediated RNAi can be a useful tool for research on the roles of genes in aphid salivary gland, and also provided new insights into the characteristics of C002 in wheat aphids.

关键词： Schizaphis graminum salivary protein C002 c DNA clone si RNA

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Genetic Variation Among the Geographic Population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China Inferred from Mitochondrial COI Gene Sequence

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Agricultural Sciences in China 2011年第7期10卷 1041-1048页

作者： XU Zhao-huan chen ju-lian chenG Deng-fa LIU Yong Frédéric Francis College of Plant Protection Shandong Agricultural University Tai＇an 271018 P.R.China State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China Functional and Evolutionary Entomology Gembloux Agro-Bio Tech University of Liege Passage des Déportés 2 Gembloux B-5030 Belgium

In order to characterize the genetic relationship of the geographic populations of Sitobion avenae （Hemiptera： Aphididae） in China, a 588 bp region of the mitochondrial cytochrome oxidase subunit I （mtDNA-COI） gene was sequenced and analyzed among the different geographic populations. 269 individuals were collected from 17 localities in different wheat-growing areas in China that covered most of the range reported for this species. Within the sequence among these geographic populations, 15 polymorphic sites defined 16 distinct haplotypes, ranging in sequence divergence from 0.2% （one nucleotide） to 1.7% （10 nucleotides）. Of the 15 variable sites, 12 were transitional substitutions, 2 were transversional substitutions and 1 was transitional and transversional substitution. Phylogenetic analysis showed that all haplotypes were highly interconnected with each other, in absence of phylogeographic structing. Each of 8 haplotypes was found only at one locality, and the other haplotypes were the widespread distributed in the different localities. The higher genetic diversity was found in the northern China populations than that in the southern China populations. The low genetic differentiation （FST=-0.06945-0.69857） and high migration rate （Nm=0.21575-infinite） of Chinese populations suggest that dispersal over long distance is a major factor in the demography of S. avenae.

关键词： Sitobion avenae insect pest mitochondrial DNA mtDNA-COI gene geographic variation

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Population genetic structure of Sitobion miscanthi in China

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Journal of Integrative Agriculture 2022年第1期21卷 178-187页

作者： SUN Jing-xuan LI Qian TAN Xiao-ling FAN Jia ZHANG Yong QIN Yao-guo Frédéric FRANCIS chen ju-lian State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Department of Functional and Evolutionary Entomology Gembloux Agro-Bio TechUniversity of LiègeGembloux 5030Belgium Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudanjiang 157000P.R.China

The wheat aphid, Sitobion miscanthi, is one of the most destructive pests of wheat plants in the temperate regions of China. Little is known about the genetic structure evolution of the different geographic populations of S. miscanthi with its migration. In this study, we investigated the population genetic structure and demographic history of S. miscanthi by analysing 18 geographical populations across China using one mitochondrial gene, COI;one nuclear gene, EF-1α;and two endosymbiont Buchnera genes, gnd and trpA. Analysis of data from the various groups showed high haplotype diversity and low nucleotide variation. SAMOVA analysis did not find a correlation between genetic distance and geographic distance. However, areas with high population diversity exhibited high haplotype diversity. Therefore, we speculate that there are two main natural migration pathways of S. miscanthi in China. One is from Yunnan to the Sichuan Basin, and the other is from Wuhan, Xinyang and Jiaodong Peninsula areas to the northwest. Based on this hypothesis, we inferred that these aphid populations appear first in the southwestern and southern regions and spread to the north with the help of the southeastern and southwestern monsoons, which occur in spring and summer. In autumn, the aphids spread southward with the northeastern and northwestern monsoons.

关键词： Sitobion miscanthi molecular marker COI symbiotic bacteria phylogenetic tree

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Influence of Garlic Intercropping or Active Emitted Volatiles in Releasers on Aphid and Related Beneficial in Wheat Fields in China

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Journal of Integrative Agriculture 2013年第3期12卷 467-473页

作者： ZHOU Hai-bo chen ju-lian LIU Yong Frédéric Francis Eric Haubruge Claude Bragard SUN Jing-rui chenG Deng-fa State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Functional and Evolutionary Entomology Gembloux Agro-Bio Tech University of Liege College of Plant Protection Shandong Agricultural University Université catholique de Louvain Earth & Life Institute Applied Microbiology-Phytopathology

In order to develop biological control of aphids by a ＂push-pull＂ approach, intercropping using repellent emitting plants was developed in different crop and associated plant models. Garlic is one of the potential plant that could be inserted in crops to decrease the pest occurrence in neighboring crop plots. In this study, field works were conducted in wheat fields in Langfang Experimental Station, Hebei Province in China from October 2009 to july 2010 during wheat developmental season. The effect of wheat intercropping with garlic but also the volatiles emission on the incidence of the English grain aphid, Sitobion avenae Fabricius （Homoptera： Aphididae） was assessed. Natural beneficial occurrence and global yields in two winter wheat varieties that were susceptible or resistant to cereal aphid were also determined comparing to control plots without the use of garlic plant intercrop nor semiochemical releaser in the fields. S. avenae was found to be lower in garlic oil blend treatment （GOB）, diallyl disulfide treatment （DD） and wheat-garlic intercropping treatment （WGI） when compared to the control plots for both two varieties （P〈0.01）. Both intercropping and application of volatile chemicals emitted by garlic could improve the population densities of natural enemies of cereal aphid, including ladybeetles and mummified aphids. Ladybeetle population density in WGI, GOB and mummified aphids densities in WGI, DD were significantly higher than those in control fields for both two varieties （P〈0.05）. There were significant interactions between cultivars and treatments to the population densities of S. avenae. The 1 000-grain weight and yield of wheat were also increased compared to the control. Due to their potential alternatives as a biological control agent against cereal aphid, garlic intercropping and related emitted volatiles are expected to contribute to the further improvement of integrated pest management systems and to potentially reduce the amount of traditio

关键词： wheat garlic intercropping semiochemical release Sitobion avenae natural enemies

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不同小麦品种(系)叶片表面蜡质对两种麦蚜取食的影响

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应用生态学报 2007年第8期18卷 1785-1788页

作者：刘勇陈巨莲程登发山东农业大学植物保护学院山东泰安271018 中国农业科学院植物保护研究所北京100094

采用气质联用(GC-MS)和生物测定法,探讨了不同小麦品种(系)叶片表面蜡质对麦长管蚜和禾谷缢管蚜取食的影响.结果表明:SN80、SN18和ZM12叶片表面蜡质对2种蚜虫取食具有刺激作用,而SN87叶片表面蜡质无刺激作用.对4种小麦材料叶片表面蜡质... 详细信息

采用气质联用(GC-MS)和生物测定法,探讨了不同小麦品种(系)叶片表面蜡质对麦长管蚜和禾谷缢管蚜取食的影响.结果表明:SN80、SN18和ZM12叶片表面蜡质对2种蚜虫取食具有刺激作用,而SN87叶片表面蜡质无刺激作用.对4种小麦材料叶片表面蜡质进行GC-MS分析发现,其表面蜡质化学组分有所不同,但主要组分均为长链烷烃,其它组分包括7-十四碳烯、8-十五烷酮、十四烷酸乙酯和十六烷酸乙酯等.生物测定结果表明:长链烷烃(>C17)、7-十四碳烯及8-十五烷酮对两种蚜虫取食具有显著的刺激作用;而乙基柠檬酸、十四烷酸乙酯和十六烷酸乙酯对麦长管蚜取食无刺激作用;十四烷酸乙酯和十六烷酸乙酯对禾谷缢管蚜取食也无刺激作用.

关键词：麦长管蚜禾谷缢管蚜小麦叶片表面蜡质

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草地贪夜蛾在不同小麦品种上的取食选择性和适应性及其与叶片生化物质含量的关系

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昆虫学报 2021年第2期64卷 230-239页

作者：刘欢张勇陈巨莲中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193

【目的】测定草地贪夜蛾Spodoptera frugiperda在不同小麦品种上的取食选择性及适应性,探索害虫为害与小麦品种间的关系,明确黄淮海麦区主栽小麦品种对草地贪夜蛾的抗性水平,为麦田抗虫品种布局和害虫综合防治提供理论依据。【方法】比... 详细信息

【目的】测定草地贪夜蛾Spodoptera frugiperda在不同小麦品种上的取食选择性及适应性,探索害虫为害与小麦品种间的关系,明确黄淮海麦区主栽小麦品种对草地贪夜蛾的抗性水平,为麦田抗虫品种布局和害虫综合防治提供理论依据。【方法】比较了草地贪夜蛾1和3龄幼虫对15个小麦品种的取食选择性,初步筛选出1和3龄幼虫取食选择性均较高的4个品种和较低的2个品种,测定了此6个品种小麦叶片的生化物质(总蛋白、可溶性糖、总酚、单宁)含量,并通过Pearson相关性分析阐明了幼虫取食选择与叶片生化成分含量间的关系,并观察了草地贪夜蛾在6个小麦品种上的适应性。【结果】草地贪夜蛾1和3龄幼虫对淮麦46、淮麦33、KOK1679和铜麦6号的取食选择性较高,而对鲁原502和烟农21的取食选择性较低。Pearson相关性分析表明,1和3龄幼虫对不同小麦品种叶片的取食选择率与小麦叶片的总蛋白和可溶性糖含量呈正相关,与叶片总酚和单宁含量呈负相关。在不同小麦品种上草地贪夜蛾的生长发育进程存在明显差异,在铜麦6号上的寄主适合度指数最高、幼虫历期最短,而在烟农21上的幼虫历期最长。【结论】草地贪夜蛾对小麦品种适应性不同,其中烟农21对其具有较高的抗性。草地贪夜蛾幼虫偏好在总蛋白和可溶性糖含量高的小麦叶片上取食,而叶片总酚和单宁含量高则会抑制其取食,说明小麦利用营养物质和次生物质的代谢协调其不选择性和抗生性,导致品种间对草地贪夜蛾抗虫性的差异。

关键词：草地贪夜蛾小麦品种取食选择性生化成分适应性生长发育繁殖力

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荻草谷网蚜唾液蛋白基因Sm13498的克隆及功能分析

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昆虫学报 2021年第9期64卷 1009-1019页

作者：付裕王倩张勇陈巨莲中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193

【目的】荻草谷网蚜Sitobion miscanthi为我国小麦Triticum aestivum主产区麦蚜优势种;Sm13498蛋白是在荻草谷网蚜唾液腺中特异表达的唾液蛋白。本研究旨在探析荻草谷网蚜功能未知的Sm13498在调节植物防御反应中的潜在作用。【方法】基... 详细信息

【目的】荻草谷网蚜Sitobion miscanthi为我国小麦Triticum aestivum主产区麦蚜优势种;Sm13498蛋白是在荻草谷网蚜唾液腺中特异表达的唾液蛋白。本研究旨在探析荻草谷网蚜功能未知的Sm13498在调节植物防御反应中的潜在作用。【方法】基于荻草谷网蚜唾液腺转录组测序数据,PCR克隆Sm13498的cDNA全长序列,并进行生物信息学分析;采用RT-qPCR测定Sm13498在取食小麦叶片不同时间的荻草谷网蚜无翅成蚜中的表达动态;通过酵母分泌系统验证Sm13498蛋白信号肽的分泌功能;利用根癌农杆菌Agrobacterium tumefaciens介导在本氏烟Nicotiana benthamiana中瞬时表达技术鉴定Sm13498蛋白功能及亚细胞定位。【结果】克隆获得了荻草谷网蚜Sm13498 cDNA全长序列(GenBank登录号:MW346655),开放阅读框(ORF)全长783 bp,编码260个氨基酸,预测蛋白分子量28.01 kD,第1-22位氨基酸为N端信号肽。系统进化树显示,Sm13498与豌豆蚜Acyrthosiphon pisum的功能未知蛋白LOC100159087 precursor(GenBank登录号:NP_001313548.1)亲缘关系最近(氨基酸序列一致性为71.7%)。RT-qPCR结果表明,Sm13498在荻草谷网蚜无翅成蚜取食小麦叶片12 h时表达水平达到最高。含有Sm13498信号肽片段的酿酒酵母Saccharomyces cerevisiae YTK12可在YPRAA培养基正常生长,并可将无色2,3,5-氯化三苯基四氮唑(TTC)还原为不可溶的暗红色的氯化三苯基四氮唑(TTF),证实其信号肽具有分泌活性。经根癌农杆菌介导在本氏烟瞬时表达Sm13498蛋白可抑制Bcl-2相关X蛋白(Bcl-2-associated X protein,BAX)及病原菌激发子INF1诱导的程序性细胞死亡。亚细胞定位结果表明,Sm13498-GFP融合蛋白定位于本氏烟叶片细胞膜。【结论】结果说明荻草谷网蚜唾液蛋白Sm13498可抑制植物防御反应。本研究为发掘荻草谷网蚜唾液中效应子,深入解析麦蚜对小麦品种强适应性奠定了基础。

关键词：荻草谷网蚜唾液蛋白效应子基因克隆信号肽亚细胞定位

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小麦蚜虫田间调查及监测技术

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应用昆虫学报 2014年第3期51卷 853-858页

作者：周海波程登发陈巨莲植物病虫害生物学国家重点实验室中国农业科学院植物保护研究所北京100193 宁夏出入境检验检疫局银川750001

小麦蚜虫是世界范围内小麦生产中一类重要害虫。针对麦蚜世代历期短、繁殖力强,具有趋光、趋化及迁飞等生物学及行为习性;在田间多呈聚集分布,且麦蚜易受寄主植物抗性、天敌、气象因素及农田生态条件等生物与非生物因素影响等发生为害特... 详细信息

小麦蚜虫是世界范围内小麦生产中一类重要害虫。针对麦蚜世代历期短、繁殖力强,具有趋光、趋化及迁飞等生物学及行为习性;在田间多呈聚集分布,且麦蚜易受寄主植物抗性、天敌、气象因素及农田生态条件等生物与非生物因素影响等发生为害特点,本文阐述了我国小麦蚜虫田间调查、监测技术及防治策略,以期为我国小麦蚜虫综合防控提供基础科学支撑。

关键词：小麦蚜虫调查监测综合治理

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产时自控镇痛与分娩方式

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中国实用医药 2010年第21期5卷 28-29页

作者：陈菊连深圳市龙岗区横岗人民医院妇产科 518115

目的孕妇于临产后使用自控硬膜外镇痛(patient controlled epidural analgesia PCEA),观察其对分娩方式的影响。方法 70例初产妇使用PCEA(A组),70例(B组)未给予任何镇痛对照,观察两组产痛程度、母儿的情况、产程进展和分娩方式。结果 1... 详细信息

目的孕妇于临产后使用自控硬膜外镇痛(patient controlled epidural analgesia PCEA),观察其对分娩方式的影响。方法 70例初产妇使用PCEA(A组),70例(B组)未给予任何镇痛对照,观察两组产痛程度、母儿的情况、产程进展和分娩方式。结果 1.用药后两组产痛评分比较有显著性差异(F=1330.312,P=0.000);2.两组第一产程时间无显著差异(P=0.6424);第二产程时间A组长于B组并有差异(P=0.0002);3.两组剖宫产率无差异(χ2=0.22P>0.05);A组阴道手术助产率明显高于B组并有显著差异(χ2=6.56,P<0.05)。结论分娩过程中使用PCEA,不影响产程进展,增加阴道手术助产率,但建议通过加强产程管理,来降低阴道手术助产率,故此技术值得在临床推广。

关键词：孕妇自控硬膜外镇痛分娩方式

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