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Global Profiles of Acetylated Proteins in Brains of Scrapie Agents 139A- and ME7-Infected Mice Collected at Mid-Early, Mid-Late, and Terminal Stages

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Biomedical and Environmental Sciences 2022年第8期35卷 722-734页

作者： SHI Qi chen dong dong ADALATI Maimaitiming XIAO Kang GAO Li Ping YANG Xue Hua WU Yue Zhang chen Cao dong Xiao Ping State Key Laboratory of Infectious Disease Prevention and Control NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBejing 102200China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang UniversityHangzhou 310014ZhejiangChina China Academy of Chinese Medical Sciences Bejing 10000China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan 43000HubeiChina Shanghai Institute of lnfectious Disease and Biosafety Shanghai 201318China

Objective To describe the global profiles of acetylated proteins in the brains of scrapie agents 139Aand ME7-infected mice collected at mid-early,mid-late,and terminal *** The acetylated proteins from the cortex regions of scrapie agent(139A-and ME7)-infected mice collected at mid-early(80 days postinfection,dpi),mid-late(120 dpi),and terminal(180 dpi) stages were extracted,and the global profiles of brain acetylated proteins were assayed with proteomic mass *** proteins in the infected mice showing 1.5-fold higher or lower levels than that of agematched normal controls were considered as differentially expressed acetylated peptides(DEAPs).Results A total of 118,42,and 51 DEAPs were found in the brains of 139A-80,139A-120,and 139A-180dpi mice,***,390,227,and 75 DEAPs were detected in the brains of ME7-80,ME7-120,and ME7-180 dpi mice,*** overwhelming majority of DEAPs in the mid-early stage were down-regulated,and more portions of DEAPs in the mid-late and late stages were *** 22.1%(328/1,485) of acetylated peptides mapped to 74 different proteins were mitochondrial *** Encyclopedia of Genes and Genomes pathway analysis identified 39(80dpi),13(120 dpi),and 10(180 dpi) significantly changed pathways in 139A-infected ***,55,25,and 18 significantly changed pathways were observed in the 80,120,and 180 dpi samples of139A-and ME7-infected mice(P < 0.05),*** pathways were commonly involved in all tested ***,many steps in the citrate cycle(tricarboxylic acid cycle) were affected,represented by down-regulated acetylation for relevant enzymes in the mid-early stage and upregulated acetylation in the mid-late and late *** Our data here illustrated the changes in the global profiles for brain acetylated proteins during prion infection,showing remarkably inhibited acetylation in the early stage and relatively enhanced acetylation in the la

关键词： Prion Scrapie-infected mouse Acetylation Proteomics Kyoto Encyclopedia of Genes and Genomes pathway

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Preparation of PrP-specific Polyclonal Antibody via Immunization of PRNP-knockout Mice with Recombinant Human PrP Protein

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Biomedical and Environmental Sciences 2020年第7期33卷 493-501页

作者： YANG Xue Hua WU Yue Zhang XIAO Kang GAO Li Ping chen dong dong dong Xiao Ping SHI Qi State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases(Zhejiang University)National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Center for Global Public Health Chinese Center for Disease Control and PreventionBeijing 102206China Wuhan Institute of Virology Chinese Academy of ScienceWuhan 430071HubeiChina China Academy of Chinese Medical Sciences Beijing 100700China

Objective The definite diagnosis of human and animal prion diseases depends on the examination of special pathological changes and/or detection of PrPSc in the brain tissues of suspected ***,developing methods to obtain PrP antibody with good specificity and sensitivity is fundamental for prion *** We prepared a PrP-specific polyclonal antibody(pAb P54)in a PRNP-knockout mouse model via immunization with recombinant full-length human PrP protein residues 23–***,we verified that pAb in Western blot,immunohistochemistry(IHC),and immunofluorescent(IFA)*** Western blot illustrated that the newly prepared pAb P54 could react with recombinant PrP protein,normal brain PrPC from healthy rodents and humans,and pathological PrPSc in the brains of experimental rodents infected with scrapie and humans infected with different types of prion *** electrophoretic patterns of brain PrPC and PrPSc observed after their reaction with pAb P54 were nearly identical to those produced by commercial PrP monoclonal *** glycosylated PrP molecules in the brain homogenates were clearly demonstrated in the reactions of these molecules with pAb *** assay revealed apparent PrP deposits in the GdnCl-treated brain slices of 139 A-infected mice and 263 K-infected *** tests with pAb P54 also showed clear green signals surrounding blue-stained cell *** The newly prepared pAb P54 demonstrated reliable specificity and sensitivity and,thus,may have potential applications not only in studies of prion biology but also in the diagnosis of human and experimental rodent prion diseases.

关键词： Prion disease PrP Antibody PRNP-knockout mouse

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Identifying the limiting factors driving the winter wheat yield gap on smallholder farms by agronomic diagnosis in North China Plain

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Journal of Integrative Agriculture 2019年第8期18卷 1701-1713页

作者： CAO Hong-zhu LI Ya-nan chen Guang-feng chen dong-dong QU Hong-rui MA Wen-qi College of Resources and Environmental Science Hebei Agricultural University Baoding 071000 P.R.China College of Resources and Environmental Sciences China Agricultural University Beijing 100193 P.R.China National Agricultural Technology Extension and Service Center Ministry of Agriculture and Rural Affairs Beijing 100125 P.R.China

North China Plain(NCP) is the primary winter wheat production region in China, characterized by smallholder farming systems. Whereas the winter wheat average yield of smallholder farmers is currently low, the yield potential and limiting factors driving the current yield gap remain unclear. Therefore, increasing the wheat yield in NCP is essential for the national food security. This study monitored wheat yield, management practices and soil nutrient data in 132 farmers’ fields of Xushui County, Baoding City, Hebei Province during 2014–2016. These data were analyzed using variance and path analysis to determine the yield gap and the contribution of yield components(i.e., spikes per hectare, grain number per spike and 1 000-grain weight) to wheat yield. Then, the limiting factors of yield components and the optimizing strategies were identified by a boundary line approach. The results showed that the attainable potential yield for winter wheat was 10 514 kg ha^–1. The yield gaps varied strongly between three yield groups(i.e., high, middle and low), which were divided by yield level and contained 44 farmers in each group, and amounted to 2 493, 1 636 and 814 kg ha^–1, respectively. For the three yield components, only spikes per hectare was significantly different(P<0.01) among the three yield groups. For all 132 farmers’ fields, correlation between yield and spikes per hectare(r=0.51, P<0.01), was significantly positive, while correlations with grain number per spike(r=–0.16) and 1 000-grain weight(r=–0.10) were not significant. The path analysis also showed that the spikes per hectare of winter wheat were the most important component to the wheat yield. Boundary line analysis showed that seeding date was the most limiting factor of spikes per hectare with the highest contribution rate(26.7%), followed by basal N input(22.1%) and seeding rate(14.5%), which indicated that management factors in the seeding step were the most important for affecting spikes per hectare.

关键词： yield gaps smallholder limiting factors path analysis boundary line analysis

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Characteristics and geological genesis of pyrites in coal of Huainan

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Journal of Coal Science & Engineering(China) 2010年第3期16卷 311-315页

作者： chen Ping JIANG dong-dong School of Earth and Environment Anhui University of Science and Technology Huainan 232001 China

Using optical microscope and scanning electron microscopy （SEM）, revealed the content of pyrite and the microscopic or sub-microscopic occurrence features of pyrite in coal of Huainan. The samples of this study are from four different mines in Huainan coal field. The results show that, in general, the coal of Huainan is low sulfur coal, but the con- tent of pyrite in Guqiao mine is relatively higher. The occurrence types of pyrite in coal mainly include： strawberry ball pyrites, massive pyrites, tuberculoid pyrite and pyrite filling in cracks or joints. After analysis based on three indicating facies parameters IAA, IS and/R, the reason for the higher level of pyrite component is that the deoxidization of swamp wa- ter is stronger, salinity is greater and the hydrodynamic is bad. The strawberry ball pyrites found in Guqiao mostly formed in the syngenesis stage. The spherical raspberry pyrites symbiotic with clay minerals was formed due to the reaction of H2S with Fe in the grid of silicate crystal.

关键词： pyrite in coal occurrence type geological genesis

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Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent LiCoO_(2)cathode material

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie Shi Changle Hou Jingjing dong dong chen Jianzhong Li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

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Developing sustainable summer maize production for smallholder farmers in the North China Plain: An agronomic diagnosis method

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Journal of Integrative Agriculture 2019年第8期18卷 1667-1679页

作者： chen Guang-feng CAO Hong-zhu chen dong-dong ZHANG Ling-bo ZHAO Wei-li ZHANG Yu MA Wen-qi JIANG Rong-feng ZHANG Hong-yan ZHANG Fu-suo College of Resources and Environmental Sciences China Agricultural University Beijing 100193 P.R.China College of Resources and Environment Science Hebei Agricultural University Baoding 071001 P.R.China Key Laboratory of Plant-Soil Interactions Ministry of Education Beijing 100193 P.R.China National Agricultural Technology Extension and Service Center Ministry of Agriculture and Rural Affairs Beijing 100125 P.R.C hina

With an increasing population and changing diet structure, summer maize is increasingly becoming an important energy crop in China. However, traditional farmer practices for maize production are inefficient and unsustainable. To ensure food security and sustainable development of summer maize production in China, an improved, more sustainable farmer management system is needed. Establishing this system requires a comprehensive understanding of the limitations of current farming practice and the ways it could be improved. In our study, 235 plots from three villages in the North China Plain(NCP) were monitored. Maize production on farms was evaluated;our results showed that the maize yield and nitrogen partial factor productivity(PFPN) were variable on smallholder farms at 6.6–13.7 t ha^–1 and 15.4–88.7 kg kg^–1, *** farming practices also have a large environmental impact(nitrogen surplus:–64.2–323.78 kg ha^–1). Key yield components were identified by agronomic diagnosis. Grain yield depend heavily on grain numbers per hectare rather than on the 1 000-grain weight. A set of improved management practices(IP) for maize production was designed by employing a boundary line(BL) approach and tested on farms. Results showed that the IP could increase yield by 18.4% and PFPN by 31.1%, compared with traditional farmer practices(FP), and reduce the nitrogen(N) surplus by 57.9 kg ha^–1. However,in terms of IP effect, there was a large heterogeneity among different smallholder farmers’ fields, meaning that, precise technologies were needed in different sites especially for N fertilizer management. Our results are valuable for policymakers and smallholder farmers for meeting the objectives of green development in agricultural production.

关键词： smallholder farmers sustainable production yield gap agronomic diagnosis North China Plain

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Dynamic self-adaptive ANP algorithm and its application to electric field simulation of aluminum reduction cell

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Journal of Central South University 2015年第12期22卷 4731-4739页

作者：王雅琳陈冬冬陈晓方蔡国民阳春华 School of Information Science and Engineering Central South University

Region partition(RP) is the key technique to the finite element parallel computing(FEPC),and its performance has a decisive influence on the entire process of analysis and *** performance evaluation index of RP method for the three-dimensional finite element model(FEM) has been *** taking the electric field of aluminum reduction cell(ARC) as the research object,the performance of two classical RP methods,which are Al-NASRA and NGUYEN partition(ANP) algorithm and the multi-level partition(MLP) method,has been analyzed and *** comparison results indicate a sound performance of ANP algorithm,but to large-scale models,the computing time of ANP algorithm increases *** is because the ANP algorithm determines only one node based on the minimum weight and just adds the elements connected to the node into the sub-region during each *** obtain the satisfied speed and the precision,an improved dynamic self-adaptive ANP(DSA-ANP) algorithm has been *** consideration of model scale,complexity and sub-RP stage,the improved algorithm adaptively determines the number of nodes and selects those nodes with small enough weight,and then dynamically adds these connected *** proposed algorithm has been applied to the finite element analysis(FEA) of the electric field simulation of *** with the traditional ANP algorithm,the computational efficiency of the proposed algorithm has been shortened approximately from 260 s to 13 *** proves the superiority of the improved algorithm on computing time performance.

关键词： finite element parallel computing(FEPC) region partition(RP) dynamic self-adaptive ANP(DSA-ANP) algorithm electric field simulation aluminum reduction cell(ARC)

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Three-dimensional simulation of sintering crunodes of metal powders or fibers by level set method

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Journal of Central South University 2015年第7期22卷 2446-2455页

作者：谌东东郑洲顺王建忠汤慧萍曲选辉 School of Mathematics and Statistics Central South University State Key Laboratory of Porous Metal Materials (Northwest Institute for Nonferrous Metal Research) School of Materials Science and Engineering University of Science and Technology Beijing

The difference of sintering crunodes of metal powders and fibers is discussed. The mathematical model of the surface diffusion described by the difference in mean curvature is defined as a Hamilton-Jacobi-type equation, and the model is numerically solved by the level set method. The three-dimensional numerical simulations of two metal powders and fibers(the fiber angle is 0° or 90°) are implemented by this mathematical model, respectively. The numerical simulation results accord with the experimental ones. The sintering neck growth trends of metal powders and metal fibers are similar. The sintering neck radius of metal fibers is larger than that of metal powders. The difference of the neck radius is caused by the difference of geometric structure which makes an important influence on the curvature affecting the migration rate of atoms.

关键词： metal fiber metal powder sintering crunodes mean curvature three-dimensional simulation

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日本感冒类汉方制剂处方标准和上市情况分析

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中草药 2021年第22期52卷 7072-7077页

作者：董晨东王勇华润三九医药股份有限公司广东深圳518110

通过梳理日本汉方目录中的感冒药并查阅日本感冒药汉方制剂的文献、官方政策与标准等,分析日本感冒药汉方制剂的处方标准,并对其上市批准文号与生产厂家进行整理。日本感冒药汉方制剂相关标准收载的内容较为详细,制剂种类与生产厂家数... 详细信息

通过梳理日本汉方目录中的感冒药并查阅日本感冒药汉方制剂的文献、官方政策与标准等,分析日本感冒药汉方制剂的处方标准,并对其上市批准文号与生产厂家进行整理。日本感冒药汉方制剂相关标准收载的内容较为详细,制剂种类与生产厂家数量较多,其中颗粒剂最为成熟。建议加快建立我国古代经典名方关键信息,对治疗常见病的经典名方上市应用给予更多关注,并为经典名方剂型开发与改善提供有益借鉴。

关键词：汉方制剂感冒药品种分析处方标准上市情况古代经典名方

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清代台湾方志中所见郑氏政权之“灾祥”研究

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福建师范大学学报（哲学社会科学版） 2013年第2期 127-132页

作者：陈冬冬华中师范大学历史文献研究所湖北武汉430079

郑氏集团是台湾历史上第一个汉人政权,在台湾历史发展中占有重要地位。清代方志中记载了数量繁多、类型丰富的有关郑氏政权的超自然的"灾祥",往往象征着政权更迭等重要史事。早期记载较为简略的"灾祥",经过比附、因果关系的构拟以及串... 详细信息

郑氏集团是台湾历史上第一个汉人政权,在台湾历史发展中占有重要地位。清代方志中记载了数量繁多、类型丰富的有关郑氏政权的超自然的"灾祥",往往象征着政权更迭等重要史事。早期记载较为简略的"灾祥",经过比附、因果关系的构拟以及串联等手法,会层累地造成情节更加复杂的"灾祥"。清代方志中有关郑氏集团的"灾祥"反映了相信"灾祥"、官方意识与民间意识混合的历史观念,也保存了不少台湾地区气象、灾荒的材料和郑氏集团的史料。

关键词：清代方志郑氏集团灾祥

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