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Novel Quartz Crystal Microbalance Biosensor for Determination of Organophosphorous Dichlorvos

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稀有金属材料与工程 2006年第A3期35卷 282-283页

作者： cuiping chen Guangzhong Xie Hongjuan Zeng Yadong Jiang Key Laboratory of Electronic Thin Films and Integrated Devices School of Opto-electrical Information University of Electronic Science ＆ Technology of China Chengdu 610054 China

A novel quartz crystal microbalance (QCM) biosensor for the determination of organophosphorus pesticide,such as dichlorvos (DDVP),has been fabricated with poly (3,4-ethylenedioxythiophene)/Poly (4-styrenesulfonate) (PEDOT/PSS) and cholinesterase butyl (BuChE).It is found that PEDOT/PSS coated sensor exhibits the highest sensitivity for DDVP among PEDOT,polyvinyl pyrrolidone,polyvinyl alcohol and polyacrylic acid sensor *** conditions to prepare PEDOT/PSS coated sensor are *** sensitivity of the PEDOT/PSS hiosensor with BuChE is higher than the others without BuChE The determination of dichlorvos in the range from 400μg/g to 2400μg/g used PEDOT/PSS with BuChE coated hiosensor was obtained.

关键词： QCM biosensor DDVP cholinesterase PEDOT/PSS

Big data challenge： a data management perspective

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Frontiers of Computer Science 2013年第2期7卷 157-164页

作者： Jinchuan chen Yueguo chen Xiaoyong DU cuiping LI Jiaheng LU Suyun ZHAO Xuan ZHOU Key Laboratory of Data Engineering and Knowledge Engineering School of Information Renmin University of China China Beijing 100872

There is a trend that, virtually everyone, rang- ing from big Web companies to traditional enterprisers to physical science researchers to social scientists, is either al- ready experiencing or anticipating unprecedented growth in the amount of data available in their world, as well as new op- portunities and great untapped value. This paper reviews big data challenges from a data management respective. In partic- ular, we discuss big data diversity, big data reduction, big data integration and cleaning, big data indexing and query, and fi- nally big data analysis and mining. Our survey gives a brief overview about big-data-oriented research and problems.

关键词： big data performance databases

Electrical stimulation of acupoint combinations against deep venous thrombosis in elderly bedridden patients after major surgery

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Journal of Traditional Chinese Medicine 2013年第2期33卷 187-193页

作者： Lili Hou cuiping chen Lei Xu PeihaoYin Wen Peng Department of Nursing Shanghai Pulmonary Hospital Department of Nursing Tenth People's Hospital of Tongji University Department of Nursing Putuo HospitalShanghai University of Chinese Medicine Department of General Surgery Putuo HospitalShanghai University of Chinese Medicine Department of Kidney Putuo HospitalShanghai University of Chinese Medicine

OBJECTIVE:To compare the effects of electrical stimulation of different acupoint combinations among postoperative bedridden elderly patients on hemorheology and deep venous blood flow velocity and investigate the role of electrical stimulation against deep vein thrombosis(DVT).METHODS:From November 2010 to October 2011,a total of 160 elderly bedridden patients after major surgery were divided into the conventional care group,invigorating and promoting Qi group,blood-activating and damp-eliminating group,and acupoint-combination stimulation *** blood viscosity,plasma viscosity,D-dimer levels,lower limb skin temperature,lower limb circumference,and flow velocities of the external iliac vein,femoral vein,popliteal vein,and deep calf veins in all patients were documented and compared among the four ***:Whole blood viscosity,plasma viscosity,D-dimer levels,and lower limb circumference were significantly reduced in the blood-activating and damp-eliminating group compared with the conventional care group(P0.05).Lower limb venous flow velocities were accelerated in the invigorating and promoting Qi group compared with the other groups,excluding the acupoint-combination stimulation group(P<0.05).CONCLUSION:Hemorheological indices in postoperative bedridden elderly patients were improved after combined electrical stimulation at Yinlingquan(SP 9) and Sanyinjiao(SP 6).Combined electrical stimulation at Zusanli(ST 36) and Taichong(LR 3),on the other hand,accelerated lower limb venous flow.

关键词： Electric stimulation Acupuncturepoint Venous thrombosis Blood flow velocity Aged Nursing care

Preparation and Characterization of PEDOT Films for NO_2 Gas Sensors

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稀有金属材料与工程 2006年第A3期35卷 178-179页

作者： Hongjuan Zeng Yadong Jiang Guangzhong Xie Junsheng Yu cuiping chen School of Optoelectronic Information University of Electronic Science and Technology of China Chengdu 610054 China

A novel NO_2 gas sensor was constructed by depositing poly(3,4-ethylenedioxyphioen)(PEDOT)films on the surface of interdigital *** conducting polymer PEDOT films were prepared by chemical *** conductibility tests indicated that the resistances of PEDOT films increased with the increase of NO_2 *** mechanism of increasing resistance of PEDOT films could be suggested that electrons of NO_2-gas acted as the electron donor to p-type semiconductor of PEDOT with the consequence of reducing the numbers of holes in the PEDOT and the increase resistance of the thin *** linear relationship between the shift of resistance and the concentration of NO_2 existed in a range from 10μg/g to 30μg/*** sensitivity of PEDOT films to NO_2 was 10Ω/10^(-6).

关键词： conducting polymer preparation characterization NO2-sensitivity

Expression and intercellular trafficking of the VP22 protein of CVI988/Rispens vaccine strain of Marek’s disease virus

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Science China(Life Sciences) 2007年第1期50卷 75-79页

作者： chen HongJun, SONG cuiping, QIN AiJian & ZHANG chenFei Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China Key Lab of Jiangsu Preventive Veterinary Medicine Yangzhou University Yangzhou China

The viral protein 22 (VP22) in the tegument of Marek’s disease virus serotype 1 (MDV-1) plays an im-portant role in cell-to-cell spread and viral propagation. Antiserum against the carboxyl terminus of VP22 was prepared by immunizing mice with recombinant VP22 expressed in E. coli, and used to in-vestigate its expression in chicken embryo fibroblast (CEF) cells infected with different MDV-1 strains. At an infection dose of PFU=50, intercellular trafficking of the VP22 into the nuclei of the surrounding receipt cells was detected as early as 3 hours post infection. By 6 hours after infection (before viral plague formation), the protein was detected in the whole nuclei of the recipient cells with no difference among MDV-1 strains CVI988/Rispens, GA and RB1B. Intra-nuclear accumulation of the VP22 protein was further increased when the viral plagues started to form. These results indicate that, albeit the ex-istence of the 201TKSERT206 deletion, the VP22 of the CVI988/Rispens vaccine strain has also intercel-lular-trafficking function, which might serve as a potential alternative delivering protein instead of virulent strains VP22.

关键词： Marek’s disease virus serotype 1 CVI988/Rispens strain VP22 intercellular trafficking

DYNAMICS OF A KIND OF RIFT VALLEY FEVER MODEL

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Annals of Differential Equations 2010年第2期26卷 127-135页

作者： Wei chen, cuiping Li (Dept. of Math. and LMIB, Beihang University, Beijing 100191)

A novel mathematical model of the epidemiology of Rift Valley fever (RVF) is studied, which is an ordinary differential equation model for a population of mosquito species and the hosts. A disease-free equilibrium is discussed as well as its local stability. The prevalence of disease is proved under some conditions. Finally the vertical transmission is considered in a model for such a mosquito population.

关键词： epidemic models vertical transmission endemic equilibrium Rift Valley fever local stability

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藜麦BADH基因家族鉴定及生物信息学分析

分子植物育种 2023年第19期21卷 6276-6284页

作者：陈翠萍青海大学农林科学院西宁810016

耐盐性是藜麦重要的优良特征之一,甜菜碱醛脱氢酶(BADH)基因是参与藜麦耐盐胁迫途径的关键酶。然而,关于藜麦BADH基因家族的研究却很少。本研究基于藜麦基因组数据库,进行藜麦BADH基因家族筛选及生物信息学分析。结果表明,藜麦共筛选到3... 详细信息

耐盐性是藜麦重要的优良特征之一,甜菜碱醛脱氢酶(BADH)基因是参与藜麦耐盐胁迫途径的关键酶。然而,关于藜麦BADH基因家族的研究却很少。本研究基于藜麦基因组数据库,进行藜麦BADH基因家族筛选及生物信息学分析。结果表明,藜麦共筛选到3个BADH基因成员,BADH蛋白氨基酸长度分别为500 aa和501 aa,蛋白分子量为54.47~54.80 kD,理论等电点为5.26~5.44,呈酸性,蛋白性质稳定,为疏水性蛋白;预测BADH蛋白亚细胞定位于叶绿体、线粒体和过氧物酶体,且无跨膜结构域,均属于ALDH-SF super family(醛脱氢酶超家族);基因结构分析表明,藜麦BADH基因家族均含有15个外显子和14个内含子,基因结构相似;系统进化分析表明,藜麦BADH与中亚滨藜、菠菜和籽粒苋同属一分支;启动子顺式作用元件分析显示,BADH基因启动子含有多个与光响应、植物激素、胁迫和植物生长相关的顺式作用元件。本研究通过生物信息学对藜麦BADH基因家族进行分析,为进一步研究BADH基因功能提供参考。

关键词：藜麦 BADH 生物信息学分析

逃亡·反抗·间离——张大春《城邦暴力团》的第一人称叙述者

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华文文学 2017年第5期 105-109页

作者：陈翠平广东技术师范学院文学与传媒学院

张大春的武侠小说《城邦暴力团》兼用第三人称和第一人称叙述视角,呈现出传奇与反传奇双重色彩。小说以完全不会武功的读书人视角,表达了现代知识分子关于隐遁和逃亡、写作的意义与局限等问题的思考。第一人称叙述者的后设叙事,则使小... 详细信息

张大春的武侠小说《城邦暴力团》兼用第三人称和第一人称叙述视角,呈现出传奇与反传奇双重色彩。小说以完全不会武功的读书人视角,表达了现代知识分子关于隐遁和逃亡、写作的意义与局限等问题的思考。第一人称叙述者的后设叙事,则使小说中以第三人称视角叙述的内容成为可疑的虚拟文本。

关键词：张大春武侠小说《城邦暴力团》第一人称叙述者

基于SSR标记的藜麦种质资源遗传多样性分析与指纹图谱构建

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江苏农业科学 2022年第13期50卷 26-31页

作者：陈翠萍刘洋青海大学农林科学院青海西宁810016 农业农村部植物新品种测试(西宁)分中心青海西宁810016

为了分析藜麦种质资源的遗传基础和遗传多样性,为藜麦种质资源的保护与高效利用提供理论依据。本研究以收集到的48份国内外藜麦种质资源为材料,采用简单重复序列(SSR)标记的方法,分析了藜麦种质资源的遗传多样性并构建DNA指纹数据库。... 详细信息

为了分析藜麦种质资源的遗传基础和遗传多样性,为藜麦种质资源的保护与高效利用提供理论依据。本研究以收集到的48份国内外藜麦种质资源为材料,采用简单重复序列(SSR)标记的方法,分析了藜麦种质资源的遗传多样性并构建DNA指纹数据库。结果表明,共筛选出25对多态性SSR分子标记,所有标记共检测到134个等位变异,每对引物检测出3~13个等位变异;Nei s基因多样性指数(H)为0.1075~0.3687,均值为0.2424;Shannon信息指数(I)为0.2043~0.5516,均值为0.3833;多态性信息含量(PIC)为0.3788~0.8522,均值为0.6042。聚类分析结果表明,48份藜麦种质资源被分为4个组,相近地区或生态环境的种质资源多聚在一组;利用3对SSR引物组合,构建了48份藜麦资源数字指纹图谱。本研究结果表明48份藜麦种质资源遗传多样性丰富;所筛选的SSR标记用于藜麦种质资源遗传多样性分析及品种鉴定是可行的。

关键词：藜麦 SSR标记遗传多样性聚类分析指纹图谱

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