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Diversity of wood-decaying fungi in Haikou Forestry Farm,Yunnan Province,***

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Studies in Fungi 2021年第1期6卷 365-377页

作者： He X chen jz Zhao CL College of Biodiversity Conservation Southwest Forestry UniversityKunming 650224P.R.China

Nine field trips carried out in Haikou Forestry Farm,Yunnan Province,*** resulted in 681 specimens of wood-decaying *** present paper summarizes 52 species collected that are distributed in 37 genera,16 families,6 orders including their hosts and substrates.A checklist of wood-decaying fungi in Haikou Forestry Farm is also *** analysis of ITS nrRNA gene region was performed for all the collected samples with maximum likelihood,maximum parsimony and Bayesian inference *** phylogenetic tree showed that fifty-two species nested in sixteen families belonging to six orders in Agaricomycetes.

关键词： Basidiomycota biological resources diversity Haikou Forestry Farm mycota

3D－QSAR　STUDY　ON　HUPERZINE　A　ANALOGS　WITH　MOLECULAR　MODELING　AND　COMPARATIVE　MOLECULAR　FIELD　ANALYSIS（COMFA）　METHODS

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Chinese Chemical Letters 1996年第3期7卷 253-256页

作者： Jiang, HL chen, KX chen, jz Tang, Y Wang, QM Li, Q Shen, X Ji, RY CHINESE ACAD SCI SHANGHAI INST MAT MEDSHANGHAI 200031PEOPLES R CHINA

On the basis of conformational search for ten huperzine A analogs according to the structure ofAChE active site witll our approach BCSPL,we performed a 3D-QSAR analysis for these compounds usingmolecular modeling and CoMFA method. The calculation results can be used to explain the relationshipbetween the structUre properties and the activities.

关键词： QSAR STUDY ON HUPERZINE ANALYSIS ANALOGS

CYCLIC　VOLTAMMETRY　STUDY　ON　ANTIMALARIAL　MECHANISM　OF　ARTEMISININ（QINGHAOSU）DERIVATIVES

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Chinese Chemical Letters 1996年第7期7卷 623-626页

作者： Jiang, HL chen, KX Tang, Y chen, jz Li, Y Wang, QM Li, Q Ji, RY Zhuang, QK CHINESE ACAD SCI SHANGHAI INST MAT MEDSHANGHAI 200031PEOPLES R CHINA BEIJING UNIV DEPT CHEMBEIJING 100871PEOPLES R CHINA

Cyclic voltammetry method waws used to determine the cyclic voltammogram of artemisininderivatives and reduction potentials(RPs)of these compounds were obtained. Combining the determinationresults of Zhang and co-workers,QSAR analysis was performed between the activity and reductionpotentials(RPs),which illustrated the antimalarial mechanism of artemisinin analogs, The experimentalresult verified and tested our previous theoretical calculation results.

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低强度次声对成骨样细胞骨桥素和骨粘连蛋白mRNA表达的作用

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中国组织工程研究与临床康复 2007年第2期11卷 271-274页

作者：王斌陈景藻刘静郭国珍解放军第四军医大学西京医院康复医学与理疗学教研室陕西省西安市710032 解放军第四军医大学放射生物学教研室陕西省西安市710032

目的:观察不同频率低强度次声对成骨细胞细胞外基质骨桥素和骨粘连蛋白mRNA的影响。方法:实验于2005-09/11在解放军第四军医大学放射生物学教研室完成。采用4个6孔培养板进行小鼠成骨样细胞系MC3T3细胞的培养,设对照组,4,12,20 Hz组4组... 详细信息

目的:观察不同频率低强度次声对成骨细胞细胞外基质骨桥素和骨粘连蛋白mRNA的影响。方法:实验于2005-09/11在解放军第四军医大学放射生物学教研室完成。采用4个6孔培养板进行小鼠成骨样细胞系MC3T3细胞的培养,设对照组,4,12,20 Hz组4组。每日上午8时分别放入次声仓内,对照组在次声仓内无次声输出,4,12,20 Hz组接受100 dB,频率为4,12,20 Hz的次声作用30 min,1次/d,连续3d。在第3天次声作用后2,4,8h分别进行成骨样细胞骨桥素及骨粘连蛋白mRNA原位杂交染色,光学显微镜下进行图像分析,测定细胞阳性物质平均灰度,进行分析。结果:①骨粘连蛋白mRNA阳性表达:次声波作用后2,4h,4,12,20 Hz组高于对照组(2h:98.3±12.4,96.3±11.2,95.9±10.4,60.3±7.9;4h:103.7±12.4,90.9±11.7,85.4±11.6,62.7±7.1;P均0.05)。②骨桥素mRNA阳性表达:次声波作用后2,4h,4,12,20 Hz组高于对照组(2h:95.4±11.2,98.3±12.9,92.3±12.7,56.6±7.2;4h:91.5±13.6,94.7±10.2,87.6±11.7,58.6±6.8;P均0.05)。结论:100 dB次声波30 min/d作用能促进成骨细胞的骨桥素及骨粘连蛋白的表达一过性增高,在4,12,20 Hz不同频率间未观察到效应差别。

关键词：声学成骨细胞细胞骨架

The Synthesis of 6-Alkyl-5-Fluorouracil Derivatives

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Chinese Chemical Letters 2000年第11期11卷 967-970页

作者： You, jz chen, SY chen, YZ Zhejiang Univ Dept Chem Hangzhou 310027 Peoples R China Lanzhou Univ Natl Lab Appl Organ Chem Lanzhou 730000 Peoples R China

6-alkyl-5-fluorouracil derivatives (5a similar to 5f) were synthesized by facile alkylation of lithiation of 5-fluorouracil derivatives with mthyl iodide (MeI) or alkyl trifluoromethanesulfonate (ROTf) in yield of 42 similar to 58%. We found that the methylated product was ethyl-substituted derivatives, not methyl-substituted derivatives.

关键词： FN MHz Cl The Synthesis of 6-Alkyl-5-Fluorouracil Derivatives

Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

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World Journal of Gastroenterology 2000年第3期6卷 365-370页

作者： Gao Y Hu HZ chen K Yang jz First Mil Med Univ Zhujiang Hosp Dept Hepatobiliary Surg Guangzhou 510282 Guangdong Provi Peoples R China

AIM To develop a culture mode providingdurable biomaterials with high yields andactivities used in bioartificial *** Hepatocytes were isolated from awhole pig liver by Seglen’s method of orthotopicperfusion with *** culture onmicrocarriers,primary porcine hepatocyteswere inoculated at a concentration of 5×10~7/mLinto the static culture systems containing 2 g/LCytodex-3,then supplemented with 100 mL/Lfetal calf serum(FCS)or 100 mL/L porcineportal vein serum(PPVS)*** aggregate culture hepatocytes wereinoculated into 100 mL siliconized flasks at aconcentration of 5.0×10~6/*** In culture on microcarriershepatocytes tended to aggregate on Cytodex-3obviously after being *** multi-cellular aggregated spheroids could be found inthe two systems 24 h-48 h after hepatocyteswere *** morphological charact-eristics and synthetic functions were maintainedfor 5 wk in FCS culture system and 8 wk in PPVSculture *** spheroidal aggregate cultureabout 80%-90% isolated hepatocytes becameaggregated spheroids 24h after cultured insuspension and mean diameter of the spheroidswas 100μ*** relationship among thehepatocytes resembled that in the liver in *** functions of albumin and urea of the spheroids were twice those of hepatocytescultured on *** As high-yields and high-activitymodes of culture on microcarriers or inspheroidal aggregate culture with portal veinserum are promising to provide biomaterials forbioartificial liver(BAL)efficiently.

关键词： porcine hepatocytes microcarriers cell culture spheroidal aggregate culture portal vein serum

Room-Temperature Synthesis of Fe-containing Mesoporous Silica

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Chinese Chemical Letters 1996年第11期7卷 1057-1058页

作者： Yuan, ZY chen, TH Wang, jz Li, HX NANKAI UNIV DEPT CHEMTIANJIN 300071PEOPLES R CHINA

A Fe-containing mesoporous silica has been synthesized at room temperature using alkylamine as templating surfactant; XRD, IR, ESR and Si-29 MAS NMR spectra provided evidence of the presence of framework and non-frame... 详细信息

关键词： Fe Room-Temperature Synthesis of Fe-containing Mesoporous Silica

Hypersensitivity and its application to structural analysis of lanthanide complexes

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Chinese Journal of Chemistry 1997年第1期15卷 49-53页

作者： Yang, W chen, M Gao, jz Yang, WD Kang, JW Yang, HW NW NORMAL UNIV DEPT PHYSLANZHOU 730070GANSUPEOPLES R CHINA

Hypersensitivity of the ternary complexes Ln(beta-dik)(4)HMQ(where Ln=Nd, Ho, Er, beta-dik=acetylacetonate(AA), dibenzoylmethanate(DBM) and MQ=4-methylquinoline) in acetone solution is studied based on the dynamic coupling model. The structures of these complexes in solution are deduced. Their coordination polyhedrons are all distorted square antiprism. Neodymium complexes have D-2 symmetry, while holmium and erbium complexes have exact D-2d symmetry.

关键词： hypersensitivity structural analysis lanthanide ternary complexes

Cloning and sequence analysis of human genomic DNA of augmenter of liver regeneration

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World Journal of Gastroenterology 2000年第2期6卷 275-277页

作者： cheng J Zhong YW Liu Y Dong J Yang jz chen JM 302 Hosp PLA Gene Therapy Res Ctr Inst Infect Dis Beijing 100039 Peoples R China

INTRODUCTIONThe liver is one of the organs,which have potentialregenerative capability in mammalian *** study of the canine model indicated that theliver could regenerate to original size after 70%hepatectomy in only ... 详细信息

关键词： augmenter liver regeneration cloning genomic DNA intron exon