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Phytochemical Characterization and Evaluation of the Antibacterial Activity of Leaf Extracts of Mitragyna inermis (Willd.) O. Ktze on the in Vitro Growth of Clinical Strains Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa Involved in Gastro Enteritis

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Journal of Biosciences and Medicines 2024年第6期12卷 101-113页

作者： Monon Kone Youssouf Zanga Traore tidiane Kamagate Ahmont Landry Claude Kablan Abdoulaye toure Karamoko Ouattara Adama Coulibaly Department of Biochemistry-Genetics Training and Research Unit Biological Sciences Peleforo Gon Coulibaly University Korhogo Cte dIvoire Department of Mathematics-Physics-Chemistry Training and Research Unit Biological Sciences Peleforo Gon Coulibaly University Korhogo Cte dIvoire Department of Biology and Health Training and Research Unit of Bioscience University of Flix Houphout-Boigny Abidjan Ivory Coast Department of Microbiology and Molecular Biology Training and Research Unit of Agriculture Fisheries Resources and Agro-Industry University of San Pedro San Pedro Cte dIvoire

Gastroenteritis constitutes a group of diarrheal diseases of infectious origin, responsible for absenteeism from work, morbidity and mortality, especially among aged people. This study aimed to evaluate the antibacterial activity of Mitragyna inermis extracts on the in vitro growth of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa involved in gastroenteritis. Phytochemical screening was carried out using two distinct methods. The detection of phytochemical compounds by tube coloring and chromatography on a thin layer of silica gel. The sensitivity of organisms was evaluated by the agar well method. The dilution method in liquid medium coupled with spreading on Mueller Hinton agar helped determine the CMB/MIC activity ratios. The investigations show that the extract has the best extraction yield (75.86% ± 0.20%) compared to the aqueous macerated (61.8% ± 0.08%) and decocted (66.6% ± 0.12%). These extracts contain several phytochemical compounds such as flavonoids, polyphenols, tannins, alkaloids, saponosides, coumarins and sterols and terpenes. These substances are endowed with biological activities and could be at the origin of antibacterial activity observed with M. inermis extracts. The analysis of antibacterial activity showed that the germs are sensitive to the extracts with inhibition diameters ranging from 8.30 ± 0.53 to 17.87 ± 0.58 mm. The ethanolic extract was the most active with diameters varying from 15.07 ± 0.62 to 17.87 ± 0.58 mm on all the germs tested. E. coli and S. aureus were the most sensitive germs to the extracts. P. aeruginosa was the least sensitive germ. Activity reports indicate that the extracts exert bactericidal activity on E. coli and S. aureus but bacteriostatic activity on P. aeruginosa. These results justify the use of M. inermis leaves in a traditional environment to treat gastroenteritis.

关键词： Phytochemical Characterization Antibacterial Activity Mitragyna inermis Gastro Enteritis

Perineal rectosigmoidectomy for strangled rectal prolapse:A case report

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Case Reports in Clinical Medicine 2014年第1期3卷 64-66页

作者： Alpha Oumar toure cheikh tidiane Diop Fode Baba toure Thomas Marcel M.Wade Gabriel Ngom Department of General Surgery Regional Hospital of OurossoguiOurossoguiSenegal Department of Pediatric Surgery Aristide Le Dantec HospitalDakarSenegal

Strangled rectal prolapse, the rare disease, is a proctological emergency. Its management is controversial. When it is not reducible and signs of ischemia are present, the Altemeierperineal rectosigmoidectomy remains the best treatment. This study aimed to report our experience on the management of strangled rectal prolapse about 1 case in a 45-year-old man, a holder of a rectal prolapse for 2 years. On admission, he had strangled prolapse for 24 hours with edema. After a vain attempt of manual reduction and installation of necrosis after 48 hours, he had an Altemeier rectosigmo?dectomy. The postoperative course was uneventful and the patient was discharged on the 6th postoperative day. The results were very good, after one year follow-up.

关键词： Strangled Rectal Prolapsed Altemeier Rectosigmoidectomy

Anatomical Dissection of the External Branch of the Superior Laryngeal Nerve to the Laboratory of Anatomy of the Faculty of Medicine of Bamako(Mali)

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Forensic Medicine and Anatomy Research 2018年第4期6卷 47-54页

作者： Abdoulaye Kante Youssouf Sidibe Babou Ba Brehima Bengaly Brehima Coulibaly Drissa Traore Drissa Ouattara Mariam Daou Tata toure Siaka Diakite Moumouna Kone Siaka Diallo Cheickh tidiane Diallo Ousmane Ibrahim toure Birama Togola Nouhoum Ongoiba Service of Surgery B CHU Point GBamakoMali Laboratory of Anatomy of the Faculty of Medicine and Odontostomatology BamakoMali ENT and Head and Neck Surgery Department CHU Mother-Child“Luxembourg”BamakoMali Service of Neurology CHU Gabriel ToureBamakMali

Aim: This study aimed at describing the anatomical variations of the external branch of the superior laryngeal nerve and at estimating the frequency of nerves at risk during the thyroid surgery. Methodology: We realized a forward-looking study from September, 2016 in May 31st, 2018 in the laboratory of anatomy of the Faculty of Medicine and Odontostomatology of Bamako in Mali. All the fresh anatomical subjects not carrying trauma and or a scar at the level of the previous region of the neck were held. The anatomical subjects were not included presenting a traumatic lesion and\or a scar of the previous region of the neck. Results: We realized 34 dissections of the external branch of the superior laryngeal nerve to 17 deathly subjects (11 men and 6 women with a sex-ratio of 1.8). The average age of the subjects was of 42 years (extremes: 18 and 70 years). Our study allowed highlighting in 100% of the cases, the external branch of the superior laryngeal nerve and the superior thyroid artery so to the right as to the left. On 34 dissected external branch of the superior laryngeal nerve, none had a previous route. However we found 28 nerves (82.4%) having a later route, stuck to the external face of the lower constrictor of the pharynx. These were not mixed with the superior thyroid artery and its branch of division and were situated outside the thyroid capsule. In 17.6% of the cases (6 cases), the nerve had a route mixed in the branch of the superior thyroid artery. These were found inside of the capsule (11.8% adhered to the artery and 5.8% crossed its branch of division). Conclusion: The risk of injury of the external laryngeal nerve during thyroid surgery procedure is never zero. It is more important on the left side.

关键词： External Laryngeal Nerve Superior Thyroid Artery Anatomy Thyroid Gland Surgery

Management of Urological Lesions Secondary to Obstetrical Gyneco Surgery in the Urology Department of the Gabriel toure University Hospital Centre

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Surgical Science 2018年第7期9卷 203-209页

作者： Amadou Berthé Mamadou tidiane Coulibaly Adama Toutou Diallo Moumine Zié Diarra Ibrahim Yattara Zanafon Outtara Amidou Domegué Ouattara Thièrno Madane Diop Urology Service Gabriel Touré University Hospital Bamako Mali Urology Service of the CHU du Point G Bamako Mali Anesthesia Unit Resuscitation at Gabriel Touré University Hospital Bamako Mali

Summarizes: The objective of this work was to study the clinical, diagnostic, therapeutic and evolutionary aspects of urological lesions secondary to gynaeco-obstetrical surgery in the urology department of the Gabriel Touré University Hospital. Material and method: It was a transversal and retrospective study carried out in the urology department of the Gabriel Touré University Hospital Centre in Bamako over 8 years. It focused on the files of 25 patients operated on for a urological lesion secondary to gynaeco-obstetrical surgery. Sociodemographic, epidemiological, diagnostic, therapeutic and evolutionary parameters were analysed. Results: Urologic lesions secondary to gynaeco-obstetrical surgery were found in 0.72% of urologically operated patients. The mean age of the patients was 39 ± 10.4 years (extremes: 18 and 60 years). The average time to diagnosis was 121, 88 ± 15 days (extremes: 0 and 365 days). Clinical signs were: oligo anuria (16%), urine leakage (52%), lumbar pain (24%). The diagnosis was made by the methylene blue test in 56% of patients, by the uro-scanner (20%) and by intravenous urography (16%). In 8% of patients, the diagnosis was made during surgery. The surgical interventions involved were: hysterectomy (48%), cesarean section (40%), genital prolapse cure (8%), ovarian cystectomy (4%). Lesions were dominated by vaginal vesico fistulas (48%) followed by ureterovaginal fistulas (20%), ureteral ligatures (16%). Treatment consisted of ureterovesical reimplantation according to Lich Gregory with ureteral intubation (36%), fistulography (48%). Healing was achieved in 92% of patients. Conclusion: Hysterectomy for cervical malignancy and cesarean section are the main etiologies of urological lesions secondary to gynaeco-obstetrical surgery. Open surgery is the only alternative for the management of these lesions in our context. Controlling anatomy is the main preventive measure.

关键词： Urologic Lesions Gyneco-Obstetrical Surgery