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黄龙病侵染增强沙糖橘对柑橘木虱吸引作用机制的初步研究

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应用昆虫学报 2024年第2期 390-400页

作者：黄克志杨玉枝闫梦梦 George Andrew charles BeATTIe 徐长宝岑伊静华南农业大学柑橘黄龙病研究室绿色农药全国重点实验室广东岭南职业技术学院 School of Science and Health Western Sydney University

【目的】黄龙病是柑橘最严重的传染性病害，柑橘木虱Diaphorina citri Kuwayama是黄龙病的媒介昆虫。与健康橘树相比，感染黄龙病的橘树对木虱的吸引作用显著增强，本研究目的是探明病树挥发物的改变是否与此现象有关。【方法】在前期... 详细信息

【目的】黄龙病是柑橘最严重的传染性病害，柑橘木虱Diaphorina citri Kuwayama是黄龙病的媒介昆虫。与健康橘树相比，感染黄龙病的橘树对木虱的吸引作用显著增强，本研究目的是探明病树挥发物的改变是否与此现象有关。【方法】在前期分析健康和染病沙糖橘Citrus reticulate ***. Shatangju嫩梢挥发物成分变化的基础上，分别在室内外测定部分差异较大的化合物单体标样及其混合物对柑橘木虱的吸引效果。【结果】雌虫对α-蒎烯（1μL/mL）、γ-萜品烯（0.01μL/mL）和芳樟醇（0.1μL/mL）具有显著的正趋性，但对柠檬烯各浓度均未表现出明显的趋性；雄虫对4种单体标样所有浓度均未表现出显著的趋性。将各种化合物按其有效浓度混合后，雌虫对6种混合物均有显著的正趋性，其中，对γ-萜品烯（0.2μL/mL）+α-蒎烯（0.8μL/mL）+芳樟醇（0.3μL/mL）具有极显著正趋向选择；雄虫对4种混合物有显著的正趋性。将化合物单体及其混合物作为诱剂，在九里香Murraya paniculata L.上测定其诱集效果。结果表明，单一物质均无显著效果，但15种混合物中有5种诱到的木虱数量显著多于对照。将其中3种混合物γ-萜品烯（20μL/mL）+α-蒎烯（80μL/mL）+芳樟醇（30μL/mL）、γ-萜品烯（2μL/mL）+α-蒎烯（8μL/mL）+芳樟醇（3μL/mL）和γ-萜品烯（20μL/mL）+芳樟醇（30μL/mL）在橘园进行测试，结果表明，3种诱剂诱到的木虱数量均显著增加。【结论】α-蒎烯、γ-萜品烯含量增加可能是沙糖橘病树对木虱吸引作用增强的部分原因，这些物质有望用于制备柑橘木虱的引诱剂，但其最佳配比还有待进一步研究。

关键词：柑橘木虱黄龙病沙糖橘寄主挥发物引诱剂

Understanding the spectrum of non-motor symptoms in multiple sclerosis:insights from animal models

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Neural Regeneration Research 2024年第1期19卷 84-91页

作者： Poornima D.e.Weerasinghe-Mudiyanselage Joong-Sun Kim Taekyun Shin Changjong Moon Department of Veterinary Anatomy and Animal Behavior College of Veterinary Medicine and BK21 FOUR programChonnam National UniversityGwangjuRepublic of Korea Department of Veterinary Anatomy College of Veterinary Medicine and Veterinary Medical Research InstituteJeju National UniversityJejuRepublic of Korea

Multiple sclerosis is a chronic autoimmune disease of the central nervous system and is generally considered to be a non-traumatic,physically debilitating neurological *** addition to experiencing motor disability,patients with multiple sclerosis also experience a variety of nonmotor symptoms,including cognitive deficits,anxiety,depression,sensory impairments,and ***,the pathogenesis and treatment of such non-motor symptoms in multiple scle rosis are still under *** studies for multiple sclerosis benefit from the use of disease-appropriate animal models,including experimental autoimmune *** to understanding the pathophysiology and developing treatments for non-motor symptoms,it is critical to chara cterize the animal model in terms of its ability to replicate certain non-motor features of multiple *** such,no single animal model can mimic the entire spectrum of *** review focuses on the non-motor symptoms that have been investigated in animal models of multiple sclerosis as well as possible underlying ***,we highlighted gaps in the literature to explain the nonmotor aspects of multiple sclerosis in expe rimental animal models,which will serve as the basis for future studies.

关键词： anxiety cognitive deficit depression experimental autoimmune encephalomyelitis motor disability neurological disorder pain pathophysiology preclinical study sensory impairments

FPR1 Antagonist (BOC-MLF) Inhibits Amniotic epithelial-mesenchymal Transition

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Current Medical Science 2024年第1期44卷 187-194页

作者： Xiao-mei HUANG e LIAO Jun-qun LIAO Ya-ling LIU Yong SHAO Department of Obstetrics and Gynecology The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070China Medical Laboratory Science The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China Department of Obstetrics Yubei Maternity and Child Healthcare HospitalChongqing 400042China

Objective:Premature rupture of membranes(PROM)is a common pregnancy disorder that is closely associated with structural weakening of fetal *** have found that formyl peptide receptor 1(FPR1)activates inflammatory pathways and amniotic epithelial-mesenchymal transition(eMT),stimulates collagen degradation,and leads to membrane weakening and membrane *** purpose of this study was to investigate the anti-inflammatory and eMT inhibitory effects of FPR1 antagonist(BOC-MLF)to provide a basis for clinical prevention of ***:The relationship between PROM,FPR1,and eMT was analyzed in human fetal membrane tissue and plasma samples using Western blotting,PCR,Masson staining,and eLISA ***(LPS)was used to establish a fetal membrane inflammation model in pregnant rats,and BOC-MLF was used to treat the LPS rat *** detected interleukin(IL)-6 in blood from the rat hearts to determine whether the inflammatory model was successful and whether the anti-inflammatory treatment was *** used electron microscopy to analyze the structure and collagen expression of rat fetal ***:Western blotting,PCR and Masson staining indicated that the expression of FPR1 was significantly increased,the expression of collagen was decreased,and eMT appeared in *** rat model indicated that LPS caused the collapse of fetal membrane epithelial cells,increased intercellular gaps,and decreased ***-MLF promoted an increase in fetal membrane collagen,inhibited eMT,and reduced the weakening of fetal ***:The expression of FPR1 in the fetal membrane of PROM was significantly increased,and eMT of the amniotic membrane was ***-MLF can treat inflammation and inhibit amniotic eMT.

关键词： formyl peptide receptor 1 BOC-MLF epithelial-mesenchymal transition premature rupture of membranes

enhancing high-temperature oxidation resistance(at 1000 °C) of nickel superalloy obtained by laser powder bed fusion via reactive electric spark treatment

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Transactions of Nonferrous Metals Society of China 2024年

作者： S.K.MUKANOV M.I.PeTRZHIK A.e.KUDRYASHOV e.A.NAUMOVA F.A.BASKOV P.A.LOGINOV e.A.LeVASHOV National University of Science and Technology MISIS

The high-temperature oxidation resistance of the nickel superalloy grown by the Laser Powder Bed Fusion (LPBF) has been significantly increased as a result of in-situ formation of a thermal barrier layer (α-Al2O3+ CaMoO4) during oxidative annealing of surface layers modified by electric spark treatment (eST). The reactive eST of the LPBF-built items based on nickel eP741NP alloy was carried out with low-melting Al-12%Si， Al-6%Ca-0.6%Si and Al-7%Ca-1%Mn electrodes. It was found that at eST done by Al-7%Ca-1%Mn electrode an intermetallic (β-NiAl + γ’-Ni3Al) 15 microns thick layer reinforced by spherical oxide (CaMe)O nanoparticles was formed. Formation of that structure increases the wear resistance of LPBF nickel superalloy by 4.5 times. Further oxidative annealing at 1000 °C lead to a formation of continuous two-layered coating with an inner layer of α-Al2O3and an outer layer of CaMoO4， which together act as an effective barrier preventing the diffusion of oxygen into the volume of the superalloy.

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Development of an active-site titrant for SARS-CoV-2 main protease as an indispensable tool for evaluating enzyme kinetics

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2349-2357页

作者： Rabea Voget Julian Breidenbach Tobias Claff Alexandra Hingst Katharina Sylvester Christian Steinebach Lan Phuong Vu Renato H.Weiße Ulrike Bartz Norbert Sträter Christa e.Müller Michael Gütschow Pharmaceutical Institute Pharmaceutical&Medicinal ChemistryUniversity of BonnBonn 53121Germany Institute of Bioanalytical Chemistry Center for Biotechnology and BiomedicineLeipzig UniversityLeipzig 04103Germany Department of Natural Sciences University of Applied Sciences Bonn-Rhein-SiegRheinbach 53359Germany

A titrant for the SARS-CoV-2 main protease(M^(pro))was developed that enables,for the first time,the exact determination of the concentration of the enzymatically active M^(pro) by active-site *** covalent binding mode of the tetrapeptidic titrant was elucidated by the determination of the crystal structure of the enzyme–titrant *** fluorogenic substrates of M^(pro),including a prototypical,internally quenched Dabcyl-eDANS peptide,were compared in terms of solubility under typical assay *** exploiting the new titrant,key kinetic parameters for the M^(pro)-catalyzed cleavage of these substrates were determined.

关键词： COVID-19 SARS-CoV-2 Main protease Peptide nitriles Fluorogenic substrates Active-site titration X-ray crystallography Inner filter effect

OsbZIP53 Negatively Regulates Immunity Response by Involving in Reactive Oxygen Species and Salicylic Acid Metabolism in Rice

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Rice science 2024年第2期31卷 190-202,I0022-I0028页

作者： WU Lijuan HAN Cong WANG Huimei He Yuchang LIN Hai WANG Lei CHeN Chen e Zhiguo State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Agricultural College of Yangzhou UniversityYangzhou 225009China

The basic region/leucine zipper(bZIP)transcription factors play important roles in plant development and responses to abiotic and biotic ***53 regulates resistance to Magnaporthe oryzae in rice by analyzing APIP5-RNAi transgenic *** further investigate the biological functions of OsbZIP53,we generated osbzip53 mutants using CRISPR/Cas9 editing and also constructed OsbZIP53 over-expression transgenic *** analysis of phenotypical,physiological,and transcriptional data showed that knocking-out OsbZIP53 not only improved disease resistance by inducing a hypersensitivity response in plants,but also regulated the immune response through the salicylic acid ***,disrupting OsbZIP53 increased H2O2 accumulation by promoting reactive oxygen species generation through up-regulation of several respiratory burst oxidase homologs(Osrboh genes)and weakened H2O2 degradation by directly targeting *** addition,the growth of osbzip53 mutants was seriously impaired,while OsbZIP53 over-expression lines displayed a similar phenotype to the wild type,suggesting that OsbZIP53 has a balancing effect on rice immune response and growth.

关键词： OsbZIP53 hypersensitive response reactive oxygen species metabolism rice immunity salicylic acid transcription factor

A systematic scoping review of study methodology for randomized controlled trials investigating probiotics in athletic and physically active populations

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Journal of Sport and Health Science 2024年第1期13卷 61-71页

作者： Alex e.Mohr David B.Pyne Geovana Silva Fogaça Leite Deborah Akins Jamie Pugh College of Health Solutions Arizona State UniversityPhoenixAZ 85004USA Research Institute for Sport and exercise University of CanberraCanberraACT 2617Australia Laboratory of Functional Fermented Food Faculty of Pharmaceutical SciencesUniversity of São PauloSão Paulo 05508-030Brazil Research Institute for Sport and exercise Sciences Liverpool John Moores UniversityLiverpool L33AFUK

Background:The purported ergogenic and health effects of probiotics have been a topic of great intrigue among researchers,practitioners,and the lay public *** has also been an increased research focus within the realm of sports science and exercise medicine on the athletic gut ***,compared to other ergogenic aids and dietary supplements,probiotics present unique study *** objectives of this systematic scoping review were to identify and characterize study methodologies of randomized controlled trials investigating supplementation with probiotics in athletes and physically active ***:Four databases(MeDLINe,CINAHL,Cochrane CeNTRAL,and Cochrane Database of Systematic Reviews)were searched for randomized controlled studies involving healthy athletes or physically active *** intervention with probiotics and inclusion of a control and/or placebo group were *** peer-reviewed articles in english were considered,and there were no date *** were extracted and presented in tabular form to detail study protocols,characteristics,and *** in randomized controlled trials was determined with the RoB 2.0 ***:A total of 45 studies were included in the review,with 35 using a parallel group design and 10 using a cross-over *** half the studies used a single probiotic and the other half a multi-strain *** probiotic dose ranged from 2×10^(8)to 1×10^(11)colony forming units daily,and the length of intervention was between 7 and 150 *** than half the studies directly assessed gastrointestinal symptoms,gut permeability,or the gut *** sex ratio of participants was heavily weighted toward males,and only 3 studies exclusively investigated ***-level adverse events were reported in only 2 studies,although the methodology of reporting varied *** risk of bias was generally low,although details on randomization were lacking in some s

关键词： exercise experimental methodology Gastrointestinal symptoms Gut microbiota Probiotic supplementation

Aging impairs the osteocytic regulation of collagen integrity and bone quality

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Bone Research 2024年第1期12卷 78-94页

作者： charles A.Schurman Serra Kaya Neha Dole Nadja M.Maldonado Luna Natalia Castillo Ryan Potter Jacob P.Rose Joanna Bons Christina D.King Jordan B.Burton Birgit Schilling Simon Melov Simon Tang eric Schaible Tamara Alliston Department of Orthopaedic Surgery University of CaliforniaSan FranciscoCA 94143USA UC Berkeley/UCSF Graduate Program in Bioengineering San FranciscoCA 94143USA Buck Institute for Research on Aging NovatoCA 94945USA Washington University in St Louis Department of OrthopedicsSt.LouisMO 63130USA Advanced Light Source Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA

Poor bone quality is a major factor in skeletal fragility in elderly *** molecular mechanisms that establish and maintain bone quality,independent of bone mass,are unknown but are thought to be primarily determined by *** hypothesize that the age-related decline in bone quality results from the suppression of osteocyte perilacunar/canalicular remodeling(PLR),which maintains bone material *** examined bones from young and aged mice with osteocyte-intrinsic repression of TGFβsignaling(TβRII^(ocy−/−))that suppresses *** control aged bone displayed decreased TGFβsignaling and PLR,but aging did not worsen the existing PLR suppression in male TβRII^(ocy−/−)*** relationship impacted the behavior of collagen material at the nanoscale and tissue scale in macromechanical *** effects of age on bone mass,density,and mineral material behavior were independent of osteocytic TGFβ.We determined that the decline in bone quality with age arises from the loss of osteocyte function and the loss of TGFβ-dependent maintenance of collagen integrity.

关键词： maintain primarily integrity

extractable and kerogen-bound hopanoids from typical eocene oil shales in China

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energy Geoscience 2024年第2期5卷 171-179页

作者： Changbo Zhai Zhirong Zhang John K.Volkman Hong Lu Yan'e Chen SINOPeC Key Laboratory of Petroleum Accumulation Mechanisms Wuxi Institute of Petroleum GeologySINOPEC2060 Lihu RoadWuxiJiangsu213126China SINOPeC Petroleum exploration and Production Research Institute 31 Xueyuan RoadBeijing100083China State Key Laboratory of Organic Geochemistry Guangzhou Institute of GeochemistryChinese Academy of ScienceGuangzhou510640GuangdongChina

This study conducted a comparative analysis of extractable hopanoid hydrocarbons and those released via stepwise pyrolysis of typical eocene immature oil shales in China,namely the Huadian,Maoming,and Fushun *** the Huadian and Maoming shales exhibit immature indicators in extractable and kerogen-bound hopanoids(notably,high abundance of C_(29)to C_(32)17β,21β-hopanes and unsaturated hopenes).In contrast,the Fushun oil shale's hopanoids from extracts and pyrolyzates suggest a higher maturity *** absence of neohopenes in the pyrolyzates of the shales underpins that the kerogenbound hopanoid skeletons resist ***,the Huadian oil shale's asymmetric distribution of C_(29)and C_(30)hopenes and neohopenes hints at the presence of an additional *** unsaturated hopenes,such as hop-20(21)-enes,identified in pyrolyzates of the three kerogens at various pyrolysis temperatures,reveal the occurrence of double bonds in kerogen-bound hopanoid skeletons without methyl *** absence of hop-20-(21)-ene in extracts suggests that it might act as an intermediate of these novel hopenes during the epimerization of hopanoid skeletons within *** extractable and pyrolytic hopanoids'stereochemical alignment indicates that epimerization may occur in both ring systems and alkyl side chains of kerogen-bound hopanoid *** stepwise pyrolysis proves to be a quick screening method for geological hopanoids without causing any significant alteration to the original skeletons even when cracking multiple covalent bonds is necessary.

关键词： Hopanoid Hopane Hopene Neohopene Stepwise pyrolysis Kerogen eocene oil shale