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Chinese Chemical letters 2015年第4期26卷 485-490页

作者： Shuai Wei charles l.brooks ⅲ Department of Chemistry University of Michigan Department of Biophysics University of Michigan

One of the main challenges of biosensor design is to understand the protein or peptide stability on the chip in high resolution structural detail. Since conventional experimental methods are limited by the resolution for their applications on surface tethered peptides/proteins, a recently developed coarse grained simulation method is employed to explore the peptide/surface interaction in residue-level resolution. This work shows how the coarse grained model successfully describes peptide-surface interactions by evaluating thermal stability of the peptide cecropin PI in bulk solution and on surfaces by physical adsorption and chemical tethering. The simulation also reproduces observations of peptide orientations on the self-assembled monolayer surface from earlier experimental work. Additionally, using knowledge obtained from the simulations, specific mutations are suggested and the desired structure and pose on the surface is obtained. In summary, this work sheds a light on the reasonable biosensor design that is ~uided by simulations.

关键词： Coarse grained simulationKB Go-like modelPeptide-surface interactionThermal stabilityOrientationMutation

Biomolecular hydration dynamics probed with 2D-IR spectroscopy:From dilute solution to a macromolecular crowd

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Chinese Chemical letters 2015年第4期26卷 435-438页

作者： John T.King Evan J.Arthur Derek G.Osborne charles l.brooks ⅲ Kevin J.Kubarych Department of Chemistry University of Michigan lSA Biophysics University of Michigan Department of Materials Science and Engineering University of Illinois

Although it is well known that water is essential for biological function, it has been a challenge to determine how water behaves near biomacromolecular interfaces, and what role water plays in influencing the dynamics of the biochemical machinery. By adopting a vibrational labeling strategy coupled with ultrafast two-dimensional infrared （2D-IR） spectroscopy, it has recently become possible to study hydration dynamics, site specifically at the surface of proteins and model membranes. We review our recent progress in measuring hydration dynamics in contexts ranging from small-molecule solutes to biomacromolecules in dilute, viscous, and crowded environments.

关键词： Protein hydration dynamicsMembrane dynamicsUltrafast infrared spectroscopy

Scalable Methods for Device Patterning as an Outstanding Challenge in Translating Paper-Based Microfluidics from the Academic Benchtop to the Point-of-Care

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Journal of Analysis and Testing 2019年第1期3卷 50-60页

作者： Jessica C.brooks charles R.Mace Department of Chemistry Tufts UniversityMedfordMA 02155USA

Paper-based microfluidic devices offer unparalleled adaptability for the development of low-cost,point-of-care analytical *** potential for these devices to drastically improve access to healthcare around the globe is obvious,but very few tests have found success in clinical ***,we identify manufacturing-specifically,methods to pattern paper devices at large scales-as a major barrier to translating prototype paper-based devices from the academic benchtop to the *** introduce current methods used to pattern papers and discuss their utility as means to prototype and manufacture paper-based devices.

关键词： Paper-based microfluidics μPAD Point-of-care Diagnostics Manufacturing

The skeleton gets a （reproductive） life

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Asian Journal of Andrology 2011年第5期13卷 651-652页

作者： charles M Allan David l Handelsman ANZAC Research Institute Concord Hospital Universityof Sydney Sydney NSW 2139 Australia

To most well-informed scientists who do not work on bone, the idea that bones determine anything other than your posture and location for the miseries of arthritis would come as quite a surprise. It would be like imagining that the walls of our home played something more than a passive role in the complexities of the lives conducted within. Yet something precisely as radical as that is what Karsenty and his colleagues boldly imagined,1 and then provocatively established in a series of studies over the past decade.20steocalcin （OCN） is a vitamin K- dependent peptide hormone secreted by the bone-formine osteoblasts.

关键词： Transcription

Advances in the management of infertility in men with spinal cord injury

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Asian Journal of Andrology 2016年第3期18卷 382-390,I0007,I0008页

作者： Emad Ibrahim Nancy l Brackett charles M lynne The Miami Project to Cure Paralysis University of Miami Miller School of Medicine Miami FL USA Department of Urology University of Miami Miller School of Medicine Miami FL USA.

Couples with a spinal cord injured male partner require assisted ejaculation techniques to collect semen that can then be further used in various assisted reproductive technology methods to achieve a pregnancy. The majority of men sustaining a spinal cord injury regardless of the cause or the level of injury cannot ejaculate during sexual intercourse. Only a small minority can ejaculate by masturbation. Penile vibratory stimulation and electroejaculation are the two most common methods used to retrieve sperm. Other techniques such as prostatic massage and the adjunct application of other medications can be used, but the results are inconsistent. Surgical sperm retrieval should be considered as a last resort if all other methods fail. Special attention must be paid to patients with T6 and rostral levels of injury due to the risk of autonomic dysreflexia resulting from stimulation below the level of injury. Bladder preparation should be performed before stimulation if retrograde ejaculation is anticipated. Erectile dysfunction is ubiquitous in the spinal cord injured population but is usually easily managed and does not pose a barrier to semen retrieval in these men. Semen analysis parameters of men with spinal cord injury are unique for this population regardless of the method of retrieval, generally presenting as normal sperm concentration but abnormally low sperm motility and viability. When sperm retrieval is desired in this population, emphasis should be placed on initially trying the simple methods of penile vibratory stimulation or electroejaculation before resorting to more advanced and invasive surgical procedures.

关键词： assisted reproductive technology ejaculation electroejaculation male infertility paraplegia penile vibratory stimulation semen sperm spinal cord injury surgical sperm retrieval

Analyzing neural degeneration of the retina with connectomics

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Neural Regeneration Research 2022年第1期17卷 113-114页

作者： charles l.Zucker John E.Dowling Department of Molecular and Cellular Biology Harvard UniversityCambridgeMAUSA

Electron microscopy(EM)provides a unique ability to visualize structural detail with a resolution orders of magnitude better than other imaging *** conventionally,its limitation is that each acquired image represents a small area with a section thickness significantly less than 100 ***,techniques have been developed that allow thousands of relatively large serial-sections to be collected and efficiently imaged at full EM resolution,with the images then being stitched back together to produce a 3D volume.

关键词： neural image Applied

Androgen deprivation therapy-associated vasomotor symptoms

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Asian Journal of Andrology 2012年第2期14卷 193-197页

作者： Jason M Jones Manish Kohli charles l loprinzi Department of Medical Oncology Mayo Clinic Rochester MN 55905 USA

Androgen deprivation therapy （ADT） is widely used as standard therapy in the treatment of locally advanced and metastatic prostate cancer. While efficacious, ADT is associated with multiple side effects, including decreased libido, erectile dysfunction, diabetes, loss of muscle tone and altered body composition, osteoporosis, lipid changes, memory loss, gynecomastia and hot flashes. The breadth of literature for the treatment of hot flashes is much smaller in men than that in women. While hormonal therapy of hot flashes has been shown to be effective, multiple non-hormonal medications and treatment methods have also been developed. This article reviews current options for the treatment of hot flashes in patients taking ADT.

关键词： androgen deprivation therapy hot flashes vasomotor symptoms

Displacement Current in Domain Walls of Bismuth Ferrite

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npj Computational Materials 2018年第1期4卷 597-605页

作者： Sergey Prosandeev Yurong Yang charles Paillard l.Bellaiche Physics Department and Institute for Nanoscience and Engineering University of ArkansasFayettevilleAR 72701USA Institute of Physics and Physics Department of Southern Federal UniversityRostov-na-Donu 344090Russia

In 1861,Maxwell conceived the idea of the displacement current,which then made laws of electrodynamics more complete and also resulted in the realization of devices exploiting such displacement ***,it is presently unknown if such displacement current can result in large intrinsic ac current in ferroic systems possessing domains,despite the flurry of recent activities that have been devoted to domains and their corresponding conductivity in these ***,we report firstprinciples-based atomistic simulations that predict that the transverse(polarization-related)displacement currents of 71°and 109°domains in the prototypical BiFeO_(3) multiferroic material are significant at the walls of such domains and in the GHz regime,and,in fact,result in currents that are at least of the same order of magnitude than previously reported dc currents(that are likely extrinsic in nature and due to electrons).Such large,localized and intrinsic ac currents are found to originate from low-frequency vibrations at the domain walls,and may open the door to the design of novel devices functioning in the GHz or THz range and in which currents would be confined within the domain wall.

关键词： currents regime magnitude

Contemporary Approaches for Identifying Rare Bone Disease Causing Genes

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Bone Research 2013年第4期1卷 301-310页

作者： charles R.Farber Thomas l.Clemens Center for Public Health Genomics University of Virginia Departments of Public Health Sciences and Biochemistry and Molecular Genetics University of Virginia Department of Orthopaedic Surgery Johns Hopkins School of Medicine Baltimore Veterans Administration Medical Center

Recent improvements in the speed and accuracy of DNA sequencing, together with increasingly sophisti- cated mathematical approaches for annotating gene networks, have revolutionized the field of human genetics and made these once time consuming approaches assessable to most investigators. In the field of bone research, a particularly active area of gene discovery has occurred in patients with rare bone disorders such as osteogenesis imperfecta （OI） that are caused by mutations in single genes. In this perspective, we highlight some of these technological advances and describe how they have been used to identify the genetic determinants underlying two previously unexplained cases of OI. The widespread availability of advanced methods for DNA sequencing and bioinformatics analysis can be expected to greatly facilitate identification of novel gene networks that normally function to control bone formation and maintenance.

关键词： exome sequencing genome sequencing bioinformatics osteogenesis imperfect pigment epithelium-derived factor (PEDF) bone restricted ifitm-like protein (Bril)

Strain control of layer-resolved negative capacitance in superlattices

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npj Computational Materials 2020年第1期6卷 127-130页

作者： Raymond Walter Sergey Prosandeev charles Paillard l.Bellaiche Department of Physics and Institute for Nanoscience and Engineering University of ArkansasFayettevilleAR 72701USA Department of Mathematical Sciences University of ArkansasFayettevilleAR 72701USA Identity Engineering Kinesso LLCConwayAR 72032USA Institute of Physics and Physics Department of Southern Federal University Rostov-na-Donu 344090Russia laboratoire Structures Propriétés et Modélisation des SolidesCentraleSupélecUMR CNRS 8580UniversitéParis-Saclay91190 Gif-sur-YvetteFrance

Negative capacitance in BaTiO_(3)/SrTiO_(3) superlattices is investigated by Monte Carlo simulations in an atomistic effective Hamiltonian model,using fluctuation formulas for responses to the local macroscopic field that incorporates depolarizing *** show that epitaxial strain can tune the negative capacitance of the BaTiO_(3) ferroelectric layer and the overall capacitance of the *** addition,we predict and explain an original switching of the negative capacitance from the BaTiO3 layer to the SrTiO_(3) layer at low temperatures for intermediate strains.

关键词： capacitance layer resolved